LOCUS       RR206727                9474 bp    DNA     circular  SYN 
DEFINITION  Slit1 (Myc-DDK-tagged ORF) - Rat slit homolog 1 (Drosophila) (Slit1), (10 ug)
ACCESSION   NM_022953
VERSION     NM_022953.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5631..5722 
                     /label="Myc-DDK"  
     misc_feature    5752..6337 
                     /label="PolyA signal"  
     misc_feature    5809..5828 
                     /label="Restriction Enzyme"  
     misc_feature    6613..7285 
                     /label="ColE1"  
     misc_feature    7715..8509 
                     /label="Kan/Neo"  
     misc_feature    8544..8846 
                     /label="SV40 ori"  
     misc_feature    8932..9409 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5613..5619 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5613 
                     /label="Slit1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCTAACG CCCCAGCGCG GGTCCTCTTC GGGGTTGAGC CGACCAGAGC 
     1081 TGTGGCTGCT GCTATGGGCA GCCGCGTGGC GCCTGGGGGC CACGGCGTGC CCCGCCCTCT 
     1141 GCACCTGCAC CGGCACTACA GTGGACTGCC ACGGCACGGG TTTGCAGGCC ATCCCCAAGA 
     1201 ACATCCCACG GAACACCGAG CGCCTGGAAC TCAATGGTAA CAATATCACT CGGATCCATA 
     1261 AGAATGACTT TGCTGGGCTC AAGCAGCTTC GAGTGTTGCA GCTGATGGAG AACCAGATTG 
     1321 GAGCTGTGGA ACGGGGAGCT TTTGATGACA TGAAGGAGTT GGAGCGGCTA CGACTGAACC 
     1381 GCAACCAGCT GCAAGTGTTG CCTGAACTAC TGTTCCAGAA CAACCAGGCT CTGTCCAGAC 
     1441 TGGACCTGAG TGAGAATTCT CTCCAGGCTG TGCCCAGAAA GGCTTTCCGA GGAGCCACAG 
     1501 ACCTCAAAAA TCTACAGCTG GACAAGAACC AGATCAGCTG CATCGAGGAA GGGGCCTTCC 
     1561 GTGCCCTACG GGGACTGGAG GTTCTGACCC TGAACAACAA CAACATCACC ACCATCCCTG 
     1621 TGTCCAGTTT CAACCATATG CCCAAGCTTC GGACCTTCCG GCTGCACTCC AACCACCTGT 
     1681 TCTGTGACTG TCACCTGGCC TGGCTCTCGC AGTGGCTGAG ACAGAGGCCC ACCATCGGGC 
     1741 TCTTCACCCA GTGCTCTGGG CCCGCCAGCC TTCGGGGCCT CAATGTGGCA GAGGTGCAAA 
     1801 AGAGTGAGTT CAGCTGCTCA GGCCAGGGGG AGGCTGCACA AGTACCTGCC TGCACCCTCT 
     1861 CATCCGGTTC CTGCCCAGCC ATGTGCAGCT GTAGCAATGG CATCGTGGAC TGCCGTGGCA 
     1921 AAGGCCTCAC TGCCATCCCT GCTAACCTTC CGGAGACGAT GACCGAGATC CGCCTGGAGC 
     1981 TGAACGGTAT CAAATCCATC CCACCAGGAG CCTTCTCTCC CTACAGAAAG CTGCGCAGGA 
     2041 TAGACCTGAG TAACAACCAG ATTGCTGAGA TTGCACCCGA TGCCTTCCAA GGCCTCCGCT 
     2101 CCTTGAATTC CCTGGTCCTC TATGGCAACA AGATCACAGA CTTGCCCCGA GGTGTATTTG 
     2161 GAGGCCTGTA CACCCTCCAG CTGCTGCTTC TGAATGCCAA CAAGATCAAC TGCATCCGGC 
     2221 CTGATGCCTT CCAGGACCTG CAGAACCTCT CTCTGCTCTC CCTATATGAC AACAAGATCC 
     2281 AGAGTCTCGC CAAGGGCACC TTCACCTCCC TGCGGGCCAT CCAGACACTG CATCTGGCCC 
     2341 AGAACCCTTT CATCTGTGAC TGTAACCTCA AGTGGCTGGC AGACTTCTTA CGTACCAACC 
     2401 CCATTGAGAC AACTGGGGCC CGCTGTGCCA GCCCCCGGCG CCTTGCCAAC AAGCGTATCG 
     2461 GGCAGATCAA AAGCAAGAAG TTCCGGTGCT CAGCCAAAGA GCAGTACTTC ATTCCAGGGA 
     2521 CGGAGGATTA CCACCTGAAC AGCGAGTGTA CGAGTGACGT GGCCTGTCCC CACAAGTGCC 
     2581 GCTGTGAGGC CAGTGTGGTG GAGTGCTCTG GCCTGAAGCT CTCTAAGATC CCTGAGCGAA 
     2641 TCCCCCAGTC CACGACTGAG CTACGACTGA ACAACAATGA GATTTCAATC CTAGAGGCCA 
     2701 CTGGGTTGTT TAAGAAGCTC TCACATCTGA AGAAAATAAA CCTGAGCAAC AACAAGGTGT 
     2761 CAGAGATTGA AGACGGGACG TTTGAAGGCG CCACCTCCGT CAGCGAGCTG CACCTGACCG 
     2821 CCAACCAGCT GGAGTCTGTC AGAAGCGGCA TGTTCCGGGG TCTAGATGGC TTGCGGACCC 
     2881 TGATGCTGAG GAACAACCGT ATCAGCTGCA TCCACAATGA CAGCTTCACT GGGCTGCGTA 
     2941 ACGTGCGGCT CCTGTCTCTT TACGACAACC ACATCACCAC CATCTCCCCT GGAGCCTTCG 
     3001 ACACCCTCCA GGCCCTGTCC ACACTAAACC TCCTGGCCAA CCCATTCAAC TGCAACTGCC 
     3061 AGTTGGCCTG GCTGGGGGAC TGGCTTCGGA AGAGGAAAAT TGTGACAGGG AACCCTCGCT 
     3121 GTCAGAACCC AGACTTCCTG CGGCAGATTC CTCTGCAAGA TGTGGCCTTC CCTGACTTCA 
     3181 GGTGTGAGGA AGGCCAGGAG GAGGTGGGCT GCCTGCCCCG CCCACAGTGC CCCCAAGAGT 
     3241 GCGCCTGCCT GGACACCGTG GTCCGGTGTA GCAACAAGCA CCTCCAGGCT CTGCCCAAGG 
     3301 GCATCCCCAA GAATGTCACA GAACTCTACT TGGATGGGAA CCAGTTCACA CTGGTTCCGG 
     3361 GACAGCTGTC TACCTTCAAG TATCTGCAGC TTGTCGACCT GAGCAACAAC AAGATCAGCT 
     3421 CCTTAAGCAA CTCTTCCTTC ACCAACATGA GTCAGCTGAC CACACTGATC CTCAGCTACA 
     3481 ACGCCCTCCA GTGCATCCCC CCTCTGGCCT TCCAAGGACT TCGTTCACTG CGCTTGCTGT 
     3541 CTTTGCATGG CAATGATGTC TCCACCCTCC AAGAGGGAAT CTTTGCAGAC GTGACGTCTC 
     3601 TGTCTCACCT GGCCATTGGT GCCAACCCCC TGTACTGCGA CTGCCACCTC CGATGGCTCT 
     3661 CCAGCTGGGT GAAGACTGGC TACAAGGAAC CTGGCATTGC CCGCTGTGCT GGACCCCCAG 
     3721 AGATGGAGGG CAAACTGTTG CTCACCACAC CTGCCAAGAA GTTTGAATGC CAAGGTCCCC 
     3781 CCAGCCTGGC GGTTCAGGCC AAGTGTGATC CCTGCTTGTC CAGCCCATGC CAGAATCAGG 
     3841 GCACTTGTCA CAATGACCCT CTTGAGGTAT ACAGGTGTAC CTGCCCCAGT GGCTACAAGG 
     3901 GCCGAAACTG TGAGGTGTCT CTGGACAGCT GTTCCAGCAA CCCATGTGGA AATGGGGGAA 
     3961 CCTGCCATGC CCAGGAGGGC GAGGATGCTG GCTTCACGTG CTCCTGTCCT TCCGGCTTTG 
     4021 AAGGGCTGAC GTGTGGAATG AACACAGATG ATTGTGTGAA GCACGACTGT GTCAATGGGG 
     4081 GTGTCTGTGT GGATGGCATA GGGAATTACA CATGTCAGTG CCCCCTGCAG TACACAGGAA 
     4141 GGGCCTGTGA ACAGCTAGTG GACTTCTGCT CTCCGGATCT GAACCCATGT CAGCATGAGG 
     4201 CCCAATGTGT TGGCACCCCA GAGGGGCCCA GGTGTGAGTG TGTGCCAGGC TACACGGGTG 
     4261 ACAATTGCAG CAAAAACCAG GACGACTGCA AGGACCACCA GTGTCAGAAC GGGGCTCAGT 
     4321 GTGTGGATGA GATCAACAGC TACGCCTGTC TCTGTGCTGA GGGCTACAGC GGACAGCTCT 
     4381 GTGAGATCCC ACCTGCCCCC AGGAACTCCT GTGAAGGGAC TGAGTGCCAG AATGGAGCCA 
     4441 ACTGTGTAGA CCAGGGCAGC CGGCCCGTAT GCCAGTGCCT ACCAGGCTTT GGAGGTCCCG 
     4501 AATGTGAGAA GTTGCTCAGT GTCAACTTTG TGGACCGGGA CACTTACCTG CAGTTCACTG 
     4561 ACCTGCAGAA CTGGCCTCGG GCCAACATCA CTCTTCAGGT CTCCACAGCA GAGGACAATG 
     4621 GGATCCTCCT CTACAATGGG GATAATGACC ACATTGCAGT TGAGCTGTAC CAGGGCCATG 
     4681 TCCGTGTTAG CTACGACCCA GGCAGCTACC CCAGCTCTGC TATCTACAGT GCTGAAACAA 
     4741 TCAACGATGG GCAGTTCCAC ACAGTTGAGC TGGTGACCTT TGACCAGATG GTGAACCTCT 
     4801 CCATTGATGG TGGCAGCCCC ATGACCATGG ACAACTTTGG AAAGCACTAC ACACTCAACA 
     4861 GTGAGGCCCC CCTCTATGTG GGAGGGATGC CCGTGGATGT GAACTCAGCT GCCTTCCGCC 
     4921 TGTGGCAGAT CCTCAATGGC ACCAGCTTCC ACGGTTGCAT CCGGAATCTA TACATCAACA 
     4981 ACGAACTGCA GGACTTCACC AAGACACAGA TGAAGCCGGG CGTGGTGCCC GGCTGCGAGC 
     5041 CCTGCCGAAA ACTCTACTGT CTACATGGCA TTTGCCAGCC CAACGCCACC CCAGGGCCCG 
     5101 TGTGCCACTG CGAGGCTGGC TGGGGGGGCC TGCACTGTGA CCAGCCAGTG GACGGCCCCT 
     5161 GCCATGGCCA CAAGTGTGTC CATGGGAAAT GCGTGCCCCT CGATGCACTT GCCTACAGCT 
     5221 GCCAATGCCA GGATGGATAC TCGGGGGCTC TGTGCAACCA GGTCGGGGCT GTGGCAGAGC 
     5281 CCTGTGGGGG CTTACAGTGC CTGCATGGTC ACTGCCAGGC CTCGGCCACC AGAGGGGCAC 
     5341 ACTGTGTGTG CAGCCCAGGT TTTTCAGGCG AGCTGTGTGA GCAAGAGTCC GAGTGCCGGG 
     5401 GGGACCCTGT CCGGGACTTT CACCGGGTCC AGAGGGGCTA TGCCATCTGC CAGACCACGC 
     5461 GCCCACTGTC ATGGGTGGAA TGCCGGGGCG CGTGCCCGGG CCAGGGCTGC TGCCAGGGCC 
     5521 TGCGGCTGAA GCGGAGGAAG CTCACCTTCG AGTGCAGCGA TGGGACCTCG TTTGCTGAGG 
     5581 AGGTGGAGAA GCCCACCAAG TGCGGCTGTG CCCCGTGTGC GACGCGTACG CGGCCGCTCG 
     5641 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5701 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5761 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5821 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5881 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5941 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     6001 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     6061 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     6121 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     6181 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     6241 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     6301 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     6361 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     6421 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     6481 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     6541 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     6601 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6661 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6721 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6781 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6841 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6901 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6961 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     7021 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     7081 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     7141 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     7201 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     7261 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     7321 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     7381 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     7441 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     7501 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     7561 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     7621 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7681 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7741 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7801 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7861 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7921 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7981 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     8041 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     8101 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     8161 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     8221 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     8281 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     8341 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     8401 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     8461 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     8521 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     8581 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     8641 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8701 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8761 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8821 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8881 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8941 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     9001 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     9061 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     9121 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     9181 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     9241 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     9301 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     9361 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     9421 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//