LOCUS       RR206718                7800 bp    DNA     circular  SYN 
DEFINITION  Nlrx1 (Myc-DDK-tagged ORF) - Rat NLR family member X1 (Nlrx1), (10 ug)
ACCESSION   NM_001025010
VERSION     NM_001025010.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3957..4048 
                     /label="Myc-DDK"  
     misc_feature    4078..4663 
                     /label="PolyA signal"  
     misc_feature    4135..4154 
                     /label="Restriction Enzyme"  
     misc_feature    4939..5611 
                     /label="ColE1"  
     misc_feature    6041..6835 
                     /label="Kan/Neo"  
     misc_feature    6870..7172 
                     /label="SV40 ori"  
     misc_feature    7258..7735 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3939..3945 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3939 
                     /label="Nlrx1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGTGGGGC TGCCATTTGC CCAGGACCTC TTGGGGCTCT GGCCTGGGAA 
     1081 GAACAGTCCC GCTCCCAGAC GAGCTCCGGA TTCAGTCGAG CTGGCCCTTT AAAGGGGTGC 
     1141 ATCATCCCCT TAGGCCCCCT AGGGCCTTTA TCCGTCACCA TGGAAACTCG GCAGACAGTG 
     1201 CTCCCCCACC AGGGAGGCAT GGGCAGCTGT TCAGGAGCAT CTCTGCCACT GAAGCTATCC 
     1261 AAAGGCATCG TCGGAACCTC ACTGAGTGGT TTAGCCGGCT GCCCAGAGAG GAGCGCCAGT 
     1321 TTGGACCAAC CTTTGCTCTA GACACAGTTC ACGTTGACCC CGTGATCCGA GAGAGCACCC 
     1381 CAGATGACCT GCTTCGTCCA TCCACGGAGC TGGCCACAGG GCATCAGCGA ACCCAGGCAG 
     1441 AGCTCCCCCC ACTGGCCCTG TCTCAGCTCT TTGACCCAGA TGCCTGTGGG CGCCGCGTGC 
     1501 AGACAGTGGT ATTGTATGGG ACCGTGGGTA CTGGCAAGAG CACATTGGTG CGCAAGATGG 
     1561 TCTTAGACTG GTGTTATGGG AGACTGCCTG CCTTTGAGCT GCTCATCCCC TTCTCCTGTG 
     1621 AGGACCTGTC ATCCCTGGGC TCCACCCCAG CTTCCTTATG CCAACTTGTG ACCCAGCGTT 
     1681 ACACACCCCT GAAAGAGGTG TTGCCCCTGA TGAATGCTGC TGGATCCCGC CTGCTCTTTG 
     1741 TGCTCCATGG CTTGGAACGC CTCAACCTTG ACTTCCGGCT GGCAGGCACA GGGCTTTGCA 
     1801 GTGACCCGGA GGAACCCGGG GCACCAGCTG CCATCATGGT CAACCTGCTG CGCAAATACA 
     1861 TGCTTCCTGA GGCCAGCATT CTGGTAACCA CCCGGCCCTC CGCCATTGGC CGTATCCCTA 
     1921 GCAAGTACGT GGGCCGCTAT GGTGAAATCT GTGGCTTCTC GGATACCAAC CTGCAGAAGC 
     1981 TCTACTTCCA GCTCCGCCTT AACCAGCCTG ACTGTGGGTA TGGTGCTGGG GGTACAGGTG 
     2041 TCTCAGTTAC ACCAGCTCAG CGGGACAATC TCATTCAAAT GCTGTCCCGA AACCTGGAGG 
     2101 GGCACCACCA GATCGCAGCC GCCTGCTTTC TGCCCTCCTA CTGCTGGCTT GTCTGTGCTA 
     2161 CCTTGCACTT CCTGCATGCT CCCACATCTG CTGGTCAGAC TCTCACAAGC ATCTATACCA 
     2221 GCTTTCTACG TCTGAACTTC AATGGGGAAA CACTGGACAG CACCGACCCC TCCAACTTAT 
     2281 CCCTGATGTC CTATGCAGCC CGGACTATGG GTAAGTTGGC CTATGAGGGG GTGTCATCCC 
     2341 GAAAGACCTA CTTCTCTGAA GAGGATGTCC GTGGCTGCCT GGAAGCTGGC ATCAAGACAG 
     2401 AGGAGGAGTT TCAGCTGCTC CAGATCTTCC GCCGGGATGC CCTGAGGTTT TTCCTGGCCC 
     2461 CGTGTGTGGA ACCAGGGCAC CTGGGTACCT TCGTATTCAC CGTACCTGCC ATGCAGGAGT 
     2521 ATCTGGCTGC CCTCTACATC GTGCTTGGTT TGCGCAAGAC AGCCCTGCAG CGGGTGGGCA 
     2581 AAGAAGTGTT TGAATTTGTG GGCCGTGTTG GGGAAGATGT CAGCCTGGTA TTGGGCATTG 
     2641 TGGCCAAACT GTTGCCCCTG CGGATTCTGC CTCTGCTATT CAACTTGCTT AAGGTGGTTC 
     2701 CACGAGTGTT TGGGCGCATG GTGAGTAAGA GTCGCGAGGC GGTGGCTCAG GCCATGGTGC 
     2761 TGGAGATGTT CCGGGAGGAA GACTATTACA ATGACGATGT TCTGGATCAG ATGGGTGCCA 
     2821 GCATCCTGGG TGTGGAGGGC CCCCGGCGCC ACCCAGATGA ACCCCCTGAG GATGAAGTCT 
     2881 TTGAGCTCTT CCCCATGTTC ATGAGTGGAC TTCTCTCTGC CCACAATCGG GCGGTGTTGG 
     2941 CTCAGCTTGG CTGTCCCATT AAGAACCTGG ATGCCCTGGA GAATGCCCAG GCAATCAAGA 
     3001 AGAAACTGGG GAAGATGGGT CGGCAGGTGC TGCCCCCCTC GGAGCTTCTT GACCATCTCT 
     3061 TCTTTCACTA TGAGTTCCAG AACCAGCGCT TCTCAGCTGA GGTGCTGGGC TCCCTACGAC 
     3121 AGCTCAATTT AGCAGGTGTG CGAATGACAC CCCTCAAGTG CACGGTGGTA GCGTCTGTAC 
     3181 TGGGAAGTGG ACGGCACCCC CTGGATGAAG TGAACTTGGC CTCCTGCCAG CTGGACCCCG 
     3241 CTGGGCTACA CACTCTCATG CCTGTTCTCC TGCGGGCCCG GAAACTGGGG TTGCAACTCA 
     3301 ACAATCTGGG CCCTGAGGCC TGCAGAGACC TCCGAGACCT GCTGTTACAT GACCAATGCC 
     3361 AGATCACCAC TCTCCGGCTG TCCAACAACC CACTGACAGC AGCAGGGGTG GGTGTGCTGA 
     3421 TGGACGGGCT GGCAGGAAAC ACTTCGGTGA CACACCTGTC CCTGCTGCAC ACTGACCTTG 
     3481 GAGATGAGGG ACTGGAGTTG CTGGCTGCCC AGCTGGACCG TAACAAACAA CTACAGGAAC 
     3541 TGAATGTGGC CTACAATGGT GCTGGGGACA CGGTGGCTCT GGCCTTGGCT AAGGCTGCTC 
     3601 GGAAGCACCC GTCCCTGGAG CTGCTGCACC TCTACTTCAA TGAGCTGAGT TCAGAGGGTC 
     3661 GCCAGGTCCT GCGGGATTTG GGCGGCTCTG GCGAAGGTGG TGCCCGGGTT GTAGCCTCGC 
     3721 TGACAGAGGG GACAGCGGTG TCTGAGTACT GGTCAGTGAT CCTCAGTGAA GTCCAACGCA 
     3781 ACCTCAACAG CTGGGACCCT GTCCGGGTCC AGCGCCATCT CAAGCTGCTG CTCCGAGACT 
     3841 TGGAAGACAG CAGAGGTGCC ACCCTTAATC CTTGGCGCAA GGCTCAGCTT CTGCGGGTGG 
     3901 AGAGCGAGGT CAAGACTCTT CTGGAGCAGC TGGGAGGCTC TGGACACACG CGTACGCGGC 
     3961 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4021 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4081 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4141 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4201 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4261 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4321 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4381 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4441 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4501 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4561 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4621 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4681 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4741 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4801 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4861 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4921 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4981 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5041 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5101 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5161 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5221 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5281 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5341 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5401 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5461 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5521 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5581 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5641 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5701 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5761 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5821 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5881 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5941 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6001 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6061 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6121 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6181 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6241 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6301 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6361 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6421 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6481 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6541 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6601 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6661 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6721 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6781 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6841 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6901 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6961 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7021 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7081 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7141 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7201 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7261 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7321 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7381 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7441 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7501 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7561 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7621 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7681 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7741 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//