LOCUS       RR206671                6624 bp    DNA     circular  SYN 
DEFINITION  Spast (Myc-DDK-tagged ORF) - Rat spastin (Spast), (10 ug)
ACCESSION   NM_001108702
VERSION     NM_001108702.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2781..2872 
                     /label="Myc-DDK"  
     misc_feature    2902..3487 
                     /label="PolyA signal"  
     misc_feature    2959..2978 
                     /label="Restriction Enzyme"  
     misc_feature    3763..4435 
                     /label="ColE1"  
     misc_feature    4865..5659 
                     /label="Kan/Neo"  
     misc_feature    5694..5996 
                     /label="SV40 ori"  
     misc_feature    6082..6559 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2763..2769 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2763 
                     /label="Spast"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTTCTCCG GCCGGACGAC GGAAGAAGAA AGGCTCAGGC GGCGCGAGCC 
     1081 CCGCGCCCGC CAGGCCTCCG CCCCCCGCCG CGGTCCCCGC CCCTGCCGCC GGCCCGGCCC 
     1141 CTGCGCCCGG CTCGCCGCAT AAGCGGAACC TGTATTACTT CTCGTATCCG CTGGTCGTCG 
     1201 GCTTCGCCCT GCTGCGCCTG CTGGCCTGCC ACCTGGGGCT CCTCTTCGTG TGGCTCTGCC 
     1261 AGCGCTTCTC CCGCGCCCTC ATGGCCGCCA AGAGGAGCTC CGGGACCGCG CCGGCGCCCG 
     1321 CCTCGCCCTC GACTCCAGCC CCCGGACCGG GTGGCGAGGC CGAGAGCGTC CGCGTCTTCC 
     1381 ACAAGCAGGC CTTTGAGTAC ATCTCCATTG CCCTGCGCAT CGACGAGGAA GAGAAAGGAC 
     1441 AGAAGGAACA AGCTGTGGAA TGGTATAAGA AAGGCATCGA AGAACTAGAG AAAGGAATCG 
     1501 CGGTTATAGT TACGGGCCAA GGTGAACAGT ACGAAAGAGC TAGACGCCTT CAAGCCAAAA 
     1561 TGATGACGAA TTTGGTTATG GCTAAGGACC GTTTACAACT CCTAGAAAGT GGAGCAGTTC 
     1621 CGAAGAAGAA AGACCCCTTA ACACATGCTA GTAATTCATT GCCTCGATCA AAAACTGTCA 
     1681 TGAAAAGTGG ATCCACAGGG CTCTCCGGTC ACCACAGGGC GCCTAGTTGC AGTGGTTTAT 
     1741 CCATGGTTTC TGGAGCAAGA CCGGGATCTG GTCCTGCAGC TACCACACAT AAGGGTACTT 
     1801 CAAAACCAAA TAGAACCAAC AAACCTTCTA CACCCACAAC TGCAGTTCGG AAAAAGAAAG 
     1861 ACTTGAAAAA TTTTAGGAAT GTGGACAGCA ATCTTGCTAA CCTTATAATG AATGAAATTG 
     1921 TTGACAATGG GACAGCTGTT AAATTTGATG ATATAGCTGG TCAGGAACTG GCAAAACAAG 
     1981 CATTGCAAGA GATTGTCATT CTCCCTTCTC TGCGGCCTGA GTTGTTCACA GGGCTCAGAG 
     2041 CTCCCGCCAG AGGCTTGTTA CTCTTCGGTC CACCAGGAAA CGGAAAAACC ATGCTGGCTA 
     2101 AAGCAGTAGC TGCAGAATCT AATGCGACTT TTTTCAATAT AAGTGCTGCA AGTTTAACTT 
     2161 CAAAATATGT AGGAGAGGGA GAGAAATTGG TGAGAGCTCT TTTTGCTGTG GCTCGAGAAC 
     2221 TTCAACCCTC TATAATTTTT ATAGATGAAG TTGATAGTCT TTTGTGTGAG AGAAGAGAAG 
     2281 GGGAGCACGA TGCTAGTAGA CGACTAAAAA CTGAATTTTT AATAGAATTT GATGGTGTGC 
     2341 AGTCTGCTGG AGATGACAGA GTCCTCGTAA TGGGTGCAAC CAACAGGCCC CAAGAGCTGG 
     2401 ATGAAGCTGT TCTCAGGCGT TTCATTAAAC GGGTGTATGT GTCATTACCC AATGAGGAGA 
     2461 CACGACTACT TCTACTTAAA AACCTGTTAT GTAAACAAGG AAGTCCACTG ACCCAAAAAG 
     2521 AACTTGCTCA ACTGGCGAGA ATGACTGATG GATACTCTGG AAGTGATCTG ACTGCTTTGG 
     2581 CAAAGGATGC AGCACTGGGT CCTATCCGAG AACTGAAACC AGAGCAGGTG AAGAACATGT 
     2641 CTGCCAGTGA GATGAGAAAT ATTCGATTAT CTGACTTCAC AGAATCCTTA AAAAAGATCA 
     2701 AACGCAGTGT GAGTCCTCAG ACTTTAGAAG CATACATACG CTGGAACAAG GACTTTGGAG 
     2761 ACACCACTGT TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     2821 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     2881 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     2941 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3001 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3061 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3121 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3181 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     3241 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     3301 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     3361 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     3421 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     3481 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     3541 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     3601 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     3661 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     3721 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     3781 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     3841 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     3901 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     3961 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4021 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4081 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4141 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4201 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     4261 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     4321 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     4381 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     4441 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     4501 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     4561 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     4621 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     4681 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     4741 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     4801 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     4861 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     4921 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     4981 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5041 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5101 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5161 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     5221 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     5281 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     5341 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     5401 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     5461 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     5521 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     5581 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     5641 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     5701 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     5761 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     5821 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     5881 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     5941 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6001 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6061 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6121 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6181 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     6241 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     6301 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     6361 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     6421 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     6481 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     6541 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     6601 TAACAAAAAT TTAACGCGAA TTTT
//