LOCUS       RR206583                7164 bp    DNA     circular  SYN 
DEFINITION  Fap (Myc-DDK-tagged ORF) - Rat fibroblast activation protein, alpha (Fap), (10 ug)
ACCESSION   NM_138850
VERSION     NM_138850.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3321..3412 
                     /label="Myc-DDK"  
     misc_feature    3442..4027 
                     /label="PolyA signal"  
     misc_feature    3499..3518 
                     /label="Restriction Enzyme"  
     misc_feature    4303..4975 
                     /label="ColE1"  
     misc_feature    5405..6199 
                     /label="Kan/Neo"  
     misc_feature    6234..6536 
                     /label="SV40 ori"  
     misc_feature    6622..7099 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3303..3309 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3303 
                     /label="Fap"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGACATGG CTGAAAACCG TCTTTGGAGT CACTACTTTG GTTGCGCTTG 
     1081 CTCTAGTGGT GATATGCATT GTCTTACGTC CCTCAAGAGT TTACAGCCCT GAAGGCAACA 
     1141 CAGGAAGATC TCTTACCCTG AAGGATATTT TAAATGGAAC ATTCTCCTAT AAAACATATT 
     1201 TTCCAAACTG GATTTCAGAG CAAGAATATC TTCATCAATC TGAGGATGAT AACATAGTCT 
     1261 TTTATAATAT TGAAACAAGA GAATCCTATA TCATTTTGAG TAATAGCACC ATGAAAAGTG 
     1321 TGAATGCTAC AGACTATGGC CTGTCACCTG ATCGGCAGTT TATATATCTT GAAAGTGATT 
     1381 ATTCAAAGCT CTGGCGATAT TCATACACAG CGACATACTA CATCTACGAC CTTCAGAATG 
     1441 GGGAATTTGT AAGAGGATAC GAGCTCCCTC GTCCAATTCA GTATCTGTGC TGGTCTCCCG 
     1501 TTGGGAGTAA ATTAGCATAT GTCTATCAAA ACAATATTTA TTTAAAACAA AGACCAGGAG 
     1561 ATCCACCTTT TCAAATAACT TACACTGGAA GAGAAAATAG AATATTTAAT GGAATTCCAG 
     1621 ACTGGGTTTA TGAAGAGGAA ATGCTTGCTA CAAAATATGC CCTTTGGTGG TCCCCAGATG 
     1681 GAAAATATTT GGCATATGTA GAATTTAATG ATTCGGACAT ACCAATTATT GCCTACTCGT 
     1741 ATTATGGCGA TGGACAGTAT CCTAGAACTA TAAATATTCC ATACCCGAAG GCCGGAGCTA 
     1801 AGAATCCCAT TGTTCGTGTC TTTATTGTTG ACACTATCTA CCCACACCAT GTGGGTCCTA 
     1861 TAGAAGTACC AGTTCCAGAG ATGATAGCCT CGAGTGACTA CTATTTCACC TGGCTCACAT 
     1921 GGGTGACCAA CGAACGAGTA TGTTTGCAGT GGCTAAAGAG AGTGCAGAAT GTCTCAGTCT 
     1981 TGTCTATATG TGATTTCAGA GAAGACTGGC ATGCGTGGGA TTGTCCAAAG AACCAGGAGC 
     2041 ATATTGAAGA AAGCAGAACA GGATGGGCTG GTGGATTCTT TGTTTCCACA CCAGCTTTTA 
     2101 GCCAGGATGC CGCTTCATAC TACAAAATAT TTAGCGACAA GGATGGTTAC AAACATATTC 
     2161 ACTACATCAA AGACACTGTG GAAAATGCTA TTCAAATTAC AAGTGGCAAG TGGGAGGCCA 
     2221 TATATATATT CCGAGTAACA CAGGATTCAC TGTTTTATTC TAGCAATGAA TTTGAAGGTT 
     2281 ACCCTGGAAG AAGAAACATC TACAGAATTA GCATTGGAAA CTCTCCTCCG AGTAAGAAGT 
     2341 GTGTTACTTG CCATCTAAGG AAAGAAAGGT GCCAATATTA CACAGCAAGT TTCAGCTACA 
     2401 AAGCCAAATA CTATGCACTC ATCTGCTACG GCCCTGGCCT CCCGATTTCC ACCCTCCATG 
     2461 ATGGCCGCAC AGACCAAGAA ATACAAGTAT TAGAAGAGAA CAAAGAACTG GAGAACGCTC 
     2521 TGAGAAACAT CCAGCTGCCT GCAGTGGAAA TTAAGAAGCT TGAAGACGGT GGAATGACTT 
     2581 TCTGGTACAA GATGATTCTT CCTCCTCAGT TTGACAGATC AAAGAAGTAC CCTCTGCTAA 
     2641 TTCAAGTGTA TGGTGGTCCC TGCAGCCAGA GTGTAAAGTC TGTATTCTCT GTTAATTGGA 
     2701 TAACTTATCT TGCAAGTAAA GAGGGGATAG TCGTTGCCTT GGTAGATGGC CGGGGCACTG 
     2761 CTTTCCAAGG TGACAAATTC CTGCATGCCG TGTATCGAAA GCTGGGTGTG TATGAAGTTG 
     2821 AGGACCAGCT CACAGCTGTC AGAAAATTCA TAGAAATGGG TTTCATTGAT GAAGGAAGAA 
     2881 TAGCCATATG GGGATGGTCC TACGGAGGTT ATGTTTCATC CCTAGCCCTT GCGTCCGGAA 
     2941 CTGGCCTTTT CAAATGTGGT ATAGCAGTGG CTCCAGTCTC CAGCTGGGAA TACTACGCCT 
     3001 CTATCTACAC GGAGAGATTC ATGGGCCTCC CAACAAAGGA TGATAATCTT GAACACTATA 
     3061 AAAATTCAAC TGTGATGGCA AGAGCAGAAT ATTTCAGAAA TGTAGACTAT CTTCTCATCC 
     3121 ACGGAACAGC AGATGATAAT GTGCACTTTC AGAACTCAGC ACAGATTGCT AAAGCTTTGG 
     3181 TTAACGCACA AGTGGATTTC CAGGCGATGT GGTACTCTGA CCAGAACCAT GGCATATCAT 
     3241 CTGGGCGCTC CCAGAACCAC TTATACACTC ACATGACGCA CTTCCTCAAG CAATGCTTTT 
     3301 CTTTATCAGA CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3361 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3421 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3481 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3541 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3601 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3661 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3721 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     3781 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     3841 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     3901 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     3961 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4021 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4081 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4141 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4201 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4261 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4321 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4381 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4441 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4501 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4561 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4621 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4681 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4741 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     4801 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     4861 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     4921 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     4981 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5041 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5101 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5161 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5221 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5281 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5341 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5401 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5461 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5521 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5581 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5641 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5701 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     5761 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     5821 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     5881 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     5941 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6001 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6061 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6121 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6181 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6241 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6301 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6361 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6421 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6481 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6541 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6601 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6661 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6721 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     6781 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     6841 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     6901 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     6961 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7021 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7081 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7141 TAACAAAAAT TTAACGCGAA TTTT
//