LOCUS       RR206477                8364 bp    DNA     circular  SYN 
DEFINITION  Kdm2a (Myc-DDK-tagged ORF) - Rat F-box and leucine-rich repeat protein 11 (Fbxl11), (10 ug)
ACCESSION   NM_001108515
VERSION     NM_001108515.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4521..4612 
                     /label="Myc-DDK"  
     misc_feature    4642..5227 
                     /label="PolyA signal"  
     misc_feature    4699..4718 
                     /label="Restriction Enzyme"  
     misc_feature    5503..6175 
                     /label="ColE1"  
     misc_feature    6605..7399 
                     /label="Kan/Neo"  
     misc_feature    7434..7736 
                     /label="SV40 ori"  
     misc_feature    7822..8299 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4503..4509 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4503 
                     /label="Kdm2a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAACCTGAA GAAGAAAGGA TTCGGTACAG CCAGAGATTG CGTGGTACCA 
     1081 TGCGACGTCG CTATGAAGAT GATGGCATTT CAGATGATGA AATTGAAGGG AAAAGAACTT 
     1141 TTGACTTGGA AGAGAAGCTC CATACCAACA AATATAATGC CAATTTTGTT ACTTTTATGG 
     1201 AGGGAAAAGA TTTTAATGTA GAGTATATCC AGCGGGGTGG CTTGAGAGAC CCTCTCATTT 
     1261 TCAAGAATTC TGATGGACTT GGAATAAAGA TGCCGGATCC AGACTTCACA GTGAATGATG 
     1321 TCAAAATGTG TGTGGGGAGT CGTCGGATGG TGGATGTCAT GGACGTGAAC ACACAGAAGG 
     1381 GGATTGAAAT GACCATGGCG CAATGGACAC GATACTATGA GACTCCAGAG GAAGAGCGAG 
     1441 AGAAACTCTA TAATGTTATC AGCCTAGAGT TTAGCCACAC CAGGCTTGAG AATATGGTAC 
     1501 AGCGGCCTTC CACGGTGGAT TTCATTGACT GGGTAGATAA CATGTGGCCA AGGCACTTGA 
     1561 AAGAAAGTCA GACTGAATCA ACAAATGCCA TCTTAGAGAT GCAGTACCCT AAAGTGCAAA 
     1621 AGTACTGTCT AATGAGTGTT CGAGGCTGCT ATACTGACTT CCATGTGGAT TTTGGAGGTA 
     1681 CTTCTGTTTG GTATCACATC CACCAAGGTG GAAAGGTCTT CTGGCTCATC CCCCCTACAG 
     1741 CCCACAACCT GGAGCTGTAC GAGAATTGGC TGCTATCAGG GAAACAGGGA GACATCTTTC 
     1801 TGGGTGACCG GGTGTCAGAT TGCCAACGAA TTGAGCTCAA GCAGGGCTAT ACCTTCGTTA 
     1861 TTCCCTCAGG TTGGATTCAT GCTGTGTATA CTCCTACGGA CACATTAGTG TTTGGAGGCA 
     1921 ATTTTTTGCA TAGCTTTAAC ATCCCCATGC AATTAAAGAT ATATAGTATT GAAGATCGAA 
     1981 CACGGGTTCC AAATAAATTC CGTTACCCAT TTTACTATGA AATGTGTTGG TATGTGTTGG 
     2041 AGCGCTATGT ATACTGCATA ACCAACCGAT CTCACCTAAC TAAGGAATTT CAGAAAGAAT 
     2101 CCCTTAGCAT GGATATGGAA TTAAATGAGT TGGAGTCTGG AAATGGTGAT GAGGAAGGGG 
     2161 CGGACAAAGA AGCCCGACGC ATGAACAGTA AGCGATCTGT GCTTACCAGC CCTGTGGCTA 
     2221 ATGGAGTCAA CCTGGATTAT GATGGACTTG GCAAAGCCTG CCGAAGTCTT CCAAATCTGA 
     2281 AGAAAACTCT GTCTGGAGAC TCATCCTCAG ACTCTAGTCG GGGATCCCAC AATGGCCAAG 
     2341 TTTGGGATCC CCAATGTAGC CCTAGAAAGG ATAGGCAAGT GCATCTGACC CACTTTGAAC 
     2401 TTGAAGGTCT TCGATGTCTT GTAGATAAGT TAGAGTCACT GCCACTGCAC AAGAAGTGTG 
     2461 TCCCCACAGG GATAGAAGAC GAAGATGCTC TGATTGCTGA TGTAAAGATT TTGCTGGAAG 
     2521 AACTTGCCAG TAGTGATCCC AAGTTAGCCC TCACTGGAGT TCCCATAGTA CAGTGGCCAA 
     2581 AAAGGGATAA GCTTAAATTT CCGACCAGGC CAAAGGTGAG GGTTCCTACA ATTCCCATCA 
     2641 CAAAGCCTCA CACCATGAAG CCGGCTCCGC GCCTAACACC TGTAAGGCCT GCTGCAGCCT 
     2701 CCCCCATTGT GTCAGGAGCC AGGCGGAGAA GAGTGCGCTG CAGGAAATGC AAAGCCTGTG 
     2761 TGCAAGGAGA ATGTGGAGTC TGCCACTACT GCAGGGACAT GAAGAAATTT GGTGGGCCTG 
     2821 GACGCATGAA GCAGTCCTGT GTCCTCAGAC AGTGCTTAGC ACCTAGACTG CCTCATTCAG 
     2881 TTACATGTTC TCTCTGTGGA GAAGTAGATC AGAATGAAGA GACACAGGAC TTTGAAAAGA 
     2941 AACTCATGGA ATGCTGCATC TGCAACGAGA TAGTTCATCC TGGCTGCCTC CAGATGGATG 
     3001 GAGAGGGGTT GCTTAACGAG GAATTGCCAA ATTGCTGGGA GTGTCCAAAG TGTTATCAGG 
     3061 AAGACAGCTC GGAGAAAGCC CAGAAGCGGA AAATAGAAGA GAGTGATGAA GAAGCTGTAC 
     3121 AAGCCAAAGT CCTACGGCCC CTGAGGAGCT GCGAGGAGCC TCTCACACCC CCGCCTCACT 
     3181 CACCTACTTC CATGCTGCAG CTCATCCACG ACCCGGTTTC TCCCCGGGGT ATGGTGACTC 
     3241 GGTCATCCCC TGGGGCTGGC CCCAGCGACC ACCACAGTGC CAGCCGTGAT GAACGCTTCA 
     3301 AACGGCGGCA GTTGCTGCGG CTACAAGCCA CAGAGCGCAC CATGGTACGG GAAAAGGAGA 
     3361 ACAATCCCAG CGGCAAAAAG GAGCTGTCTG AAGTTGAGAA AGCCAAGATC CGGGGATCGT 
     3421 ACCTCACTGT CACGCTACAG AGGCCCACCA AAGAGCTCCA CGGGACATCC ATTGTGCCCA 
     3481 AGCTGCAGGC CATCACGGCC TCCTCTGCCA ACCTTCGCCC TAACCCCCGT GTGCTAATGC 
     3541 AGCACTGCCC AGCCCGGAAC CCCCAGCGTG GGGATGAGGA GGGGCTTGGG GGAGAGGAGG 
     3601 AGGAAGAGGA GGAGGAGGAG GAAGATGACA GTGCAGAGGA GGGGGGTGCA GCAAGGCTGA 
     3661 ATGGCCGGGG CAGTTGGGCT CAGGATGGAG ACGAAAGCTG GATGCAGCGG GAGGTCTGGA 
     3721 TGTCTGTCTT CCGCTACCTC AGCCGCAGAG AACTTTGTGA ATGTATGCGA GTGTGCAAGA 
     3781 CGTGGTATAA ATGGTGCTGT GACAAACGAC TTTGGACAAA AATTGACTTG AGTAGGTGTA 
     3841 AGGCCATCGT GCCCCAGGCT CTCAGTGGCA TCATCAAGCG GCAGCCAGTA AGCCTGGACC 
     3901 TTAGTTGGAC TAACATCTCC AAAAAGCAGC TGACATGGCT GGTCAATAGG CTGCCAGGAT 
     3961 TAAAAGACCT CCTCCTAGCA GGCTGTTCCT GGTCTGCAGT ATCTGCCCTC AGCACCTCCA 
     4021 GCTGCCCCCT TCTCAGGACC CTTGATCTTC GGTGGGCAGT AGGAATTAAA GACCCTCAAA 
     4081 TTCGGGACTT GCTGACTCCA CCCACAGATA AGCCAGGTCA AGACAATCGA AGCAAACTCC 
     4141 GGAACATGAC TGACTTCCGG CTGGCAGGCC TTGACATCAC CGATGCCACT CTCCGACTCA 
     4201 TTATTCGCCA CATGCCCCTT TTGTCTCGAC TTGACCTCAG TCACTGCAGC CACCTTACAG 
     4261 ATCAGTCCTC CAACCTGCTC ACTGCTGTCG GGTCTTCCAC TCGATACTCC CTTACAGAGC 
     4321 TCAACATGGC GGGTTGCAAT AAACTGACAG ACCAGACCCT GTTCTTCCTA AGGAGAATTG 
     4381 CTAATGTCAC CTTGATTGAC CTTCGAGGAT GCAAACAGAT CACAAGAAAA GCCTGTGAGC 
     4441 ACTTCATCTC AGACTTGTCC ATCAACAGCC TCTACTGCCT GTCTGATGAG AAACTGATAC 
     4501 AGAAGATTAG CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4561 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4621 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4681 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4741 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4801 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4861 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4921 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4981 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5041 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5101 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5161 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5221 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5281 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5341 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5401 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5461 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5521 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5581 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5641 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5701 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5761 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5821 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5881 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5941 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6001 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6061 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6121 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6181 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6241 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6301 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6361 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6421 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6481 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6541 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6601 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6661 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6721 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6781 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6841 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6901 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6961 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7021 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7081 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7141 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7201 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7261 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7321 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7381 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7441 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7501 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7561 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7621 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7681 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7741 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7801 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7861 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7921 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7981 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8041 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8101 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8161 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8221 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8281 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8341 TAACAAAAAT TTAACGCGAA TTTT
//