LOCUS       RR206428                7311 bp    DNA     circular  SYN 
DEFINITION  Ubc (Myc-DDK-tagged ORF) - Rat ubiquitin C (Ubc), (10 ug)
ACCESSION   NM_017314
VERSION     NM_017314.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3468..3559 
                     /label="Myc-DDK"  
     misc_feature    3589..4174 
                     /label="PolyA signal"  
     misc_feature    3646..3665 
                     /label="Restriction Enzyme"  
     misc_feature    4450..5122 
                     /label="ColE1"  
     misc_feature    5552..6346 
                     /label="Kan/Neo"  
     misc_feature    6381..6683 
                     /label="SV40 ori"  
     misc_feature    6769..7246 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3450..3456 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3450 
                     /label="Ubc"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGATCTTT GTGAAAACCT TAACTGGTAA GACCATCACC CTGGAGGTCG 
     1081 AGCCCAGTGA CACCATTGAA AATGTCAAGG CAAAGATCCA GGACAAGGAG GGCATCCCCC 
     1141 CTGACCAGCA GAGGCTGATC TTTGCAGGCA AGCAGCTGGA AGATGGCCGC ACCCTGTCAG 
     1201 ACTACAACAT CCAGAAGGAG TCCACCCTGC ACCTGGTCCT GCGCCTCAGA GGGGGCATGC 
     1261 AGATCTTTGT GAAGACCCTG ACAGGCAAGA CCATCACCCT GGAGGTCGAG CCCAGTGACA 
     1321 CCATTGAAAA TGTCAAGGCA AAGATCCAGG ACAAGGAGGG CATCCCCCCT GACCAGCAGA 
     1381 GGCTGATCTT TGCAGGCAAG CAGCTGGAAG ATGGCCGCAC CCTGTCAGAC TACAACATCC 
     1441 AGAAGGAGTC CACCTTGCAC CTGGTCCTTC GCCTCAGAGG GGGCATGCAG ATCTTTGTGA 
     1501 AGACCCTGAC AGGCAAGACC ATCACCCTGG AGGTCGAGCC CAGTGACACC ATTGAAAATG 
     1561 TCAAGGCAAA GATCCAGGAC AAGGAGGGCA TCCCCCCTGA CCAGCAGAGG CTGATCTTTG 
     1621 CAGGCAAGCA GCTGGAAGAT GGCCGCACCC TGTCAGACTA CAACATCCAG AAGGAGTCCA 
     1681 CCCTGCACCT GGTCCTTCGC CTCAGAGGGG GCATGCAGAT CTTTGTGAAG ACCCTGACAG 
     1741 GCAAGACCAT CACCCTGGAG GTCGAGCCCA GTGACACCAT TGAAAATGTC AAGGCAAAGA 
     1801 TCCAGGACAA GGAGGGCATC CCCCCTGACC AGCAGAGGCT GATCTTTGCA GGCAAGCAGC 
     1861 TGGAAGATGG CCGCACCCTG TCAGACTACA ACATCCAGAA GGAGTCCACC CTGCACCTGG 
     1921 TCCTGCGCCT CAGAGGGGGC ATGCAGATCT TTGTGAAGAC CCTGACAGGC AAGACCATCA 
     1981 CCCTGGAGGT CGAGCCCAGT GACACCATTG AAAATGTCAA GGCAAAGATC CAGGACAAGG 
     2041 AGGGCATCCC CCCTGACCAG CAGAGGCTGA TCTTTGCAGG CAAGCAGCTG GAAGATGGCC 
     2101 GCACCCTGTC AGACTACAAC ATCCAGAAGG AGTCCACCCT GCACCTGGTC CTTCGCCTCA 
     2161 GAGGGGGCAT GCAGATCTTT GTGAAGACCC TGACAGGCAA GACCATCACC CTGGAGGTCG 
     2221 AGCCCAGTGA CACCATTGAA AATGTCAAGG CAAAGATCCA GGACAAGGAG GGCATCCCCC 
     2281 CTGACCAGCA GAGGCTGATC TTTGCAGGCA AGCAGCTGGA AGATGGCCGC ACCCTGTCAG 
     2341 ACTACAACAT CCAGAAGGAG TCCACCCTGC ACCTGGTCCT TCGCCTCAGA GGGGGCATGC 
     2401 AGATCTTTGT GAAGACCCTG ACAGGCAAGA CCATCACCCT GGAGGTCGAG CCCAGTGACA 
     2461 CCATTGAAAA TGTCAAGGCA AAGATCCAGG ACAAGGAGGG CATCCCCCCT GACCAGCAGA 
     2521 GGCTGATCTT TGCAGGCAAG CAGCTGGAAG ATGGCCGCAC CCTGTCAGAC TACAACATCC 
     2581 AGAAGGAGTC CACCCTGCAC CTGGTCCTTC GCCTCAGAGG GGGCATGCAG ATCTTTGTGA 
     2641 AGACCCTGAC AGGCAAGACC ATCACCCTGG AGGTCGAGCC CAGTGACACC ATTGAAAATG 
     2701 TCAAGGCAAA GATCCAGGAC AAGGAGGGCA TCCCCCCTGA CCAGCAGAGG CTGATCTTTG 
     2761 CAGGCAAGCA GCTGGAAGAT GGCCGCACCC TGTCAGACTA CAACATCCAG AAGGAGTCCA 
     2821 CCCTGCACCT GGTCCTGCGC CTCAGAGGGG GCATGCAGAT CTTTGTGAAG ACCCTGACAG 
     2881 GCAAGACCAT CACCCTGGAG GTCGAGCCCA GTGACACCAT TGAAAATGTC AAGGCAAAGA 
     2941 TCCAGGACAA GGAGGGCATC CCCCCTGACC AGCAGAGGCT GATCTTTGCA GGCAAGCAGC 
     3001 TGGAAGATGG CCGCACCCTG TCAGACTACA ACATCCAGAA GGAGTCCACC CTGCACCTGG 
     3061 TCCTGCGCCT CAGAGGGGGC ATGCAGATCT TTGTGAAGAC CCTGACAGGC AAGACCATCA 
     3121 CCCTGGAGGT CGAGCCCAGT GACACCATTG AAAATGTCAA GGCAAAGATC CAGGACAAGG 
     3181 AGGGCATCCC CCCTGACCAG CAGAGGCTGA TCTTTGCAGG CAAGCAGCTG GAAGATGGCC 
     3241 GCACCCTGTC AGACTACAAC ATCCAGAAGG AGTCCACCCT GCACCTGGTC CTGCGCCTCA 
     3301 GAGGGGGCAT GCAGATCTTT GTGAAGACCC TGACAGGCAA GACCATCACT CTGGAGGTCG 
     3361 AGCCCAGTGT TAACACCAAG AAGGTCAAAC AGGAAGATAC TCGTACCTTT CTCACCACAG 
     3421 TATCTAGAAA GAGCCCTTCT TGTGCTTGTT CTTGGGTTAC GCGTACGCGG CCGCTCGAGC 
     3481 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     3541 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3601 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3661 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     3721 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     3781 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     3841 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     3901 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     3961 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4021 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4081 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4141 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4201 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4261 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4321 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4381 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4441 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4501 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     4561 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     4621 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     4681 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     4741 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     4801 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     4861 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     4921 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     4981 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5041 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5101 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5161 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5221 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5281 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     5341 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5401 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     5461 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     5521 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     5581 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     5641 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     5701 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     5761 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     5821 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     5881 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     5941 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6001 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6061 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6121 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6181 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6241 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6301 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6361 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6421 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6481 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     6541 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6601 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     6661 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     6721 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     6781 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     6841 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     6901 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     6961 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7021 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7081 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7141 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7201 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7261 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//