LOCUS       RR206402                8790 bp    DNA     circular  SYN 
DEFINITION  Insrr (Myc-DDK-tagged ORF) - Rat insulin receptor-related receptor (Insrr), (10 ug)
ACCESSION   NM_022212
VERSION     NM_022212.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4945..5036 
                     /label="Myc-DDK"  
     misc_feature    5066..5651 
                     /label="PolyA signal"  
     misc_feature    5123..5142 
                     /label="Restriction Enzyme"  
     misc_feature    5927..6599 
                     /label="ColE1"  
     misc_feature    7029..7823 
                     /label="Kan/Neo"  
     misc_feature    7858..8160 
                     /label="SV40 ori"  
     misc_feature    8246..8723 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4920..4927 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4920 
                     /label="Insrr"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGTGCCT GCTCTGTGGC CCTGGGGAGT ATACTTGCTT ATGAGCCTGC 
     1081 TGTCCTTGGG ATCTTGCCTG GACACACTGG AGGTGTGCCC CAGTCTTGAC ATCCGCTCGG 
     1141 AGGTGACAGA GCTCCGTCGG CTGGAGAACT GCAGTGTGGT GGAGGGTCAC CTACAGATCC 
     1201 TCCTCATGTT TGCCGCCACG GGAGAGGACT TCCGAGGCCT CAGCTTTCCT CGCCTCACCC 
     1261 AGGTGACTGA CTACCTGCTG CTGTTCCGAG TCTATGGCCT AGAGAGCCTT CGAGACCTCT 
     1321 TCCCCAACCT TGCTGTGATC CGAGGTGCGC GCCTCTTCCT GGGCTATGCA CTTATAATCT 
     1381 TCGAGATGCC CCACTTGAGG GACATTGGGC TGCCGTCGCT TGGGGCCGTG CTGCGTGGGG 
     1441 CCGTTCGTGT GGAGAAGAAC CAGGAACTTT GCCATCTCTC CACCATTGAC TGGGGCCTGC 
     1501 TGCAGCCTGC ACCTGGTGCC AACCACATTG TTGGGAACAA GCTGGGTGAG GAGTGTGCTG 
     1561 ACGTGTGCCC TGGTGTGCTG GGGGCTGCTG GTGAGCCCTG TGTGAGAACC ACCTTCGGTG 
     1621 GGCACACCGA CTACAGGTGT TGGACCTCCA GCCACTGTCA GAGAGTGTGT CCCTGCCCCC 
     1681 GTGGGCTGGC CTGCACAGTA GGTGGCGAGT GCTGCCACAG TGAATGTCTC GGGGGCTGCA 
     1741 GTCAGCCTGA AGACCCTCGA GCCTGCGTAG CTTGCCGCCA CCTCTACTTC CAAGGAGTCT 
     1801 GCCTCCCCAC CTGCCCTCCG GGCACCTACC AGTATGAGTC TTGGCGGTGT GTCACTGCGG 
     1861 AGCTCTGTGG CCATCTGCGA GAGGTTCCTG GACATGCCAC CGCCTTTGGC ATCTACGAGG 
     1921 GCAGCTGCCT AGCTCAGTGC CCTCCAGGCT TCACCCGTAA TGGCAGCAGC ATTTTCTGCC 
     1981 ATAAGTGTGA GGGCCTGTGC CCCAAAGAGT GCAAGGTTGG GACAAAGACC ATCGACTCTG 
     2041 TCCAAGCCAC ACAGGACCTA GTGGGCTGCA CCCATGTGGA GGGGAGCCTC ATCCTCAACC 
     2101 TTCGACAAGG CTACAATCTG GAGCCAGAGC TTCAACGCAA CCTGGGGCTG GTGGAGACCA 
     2161 TCACCGGCTT CCTCAAAATC AAGCACTCTT TTGCACTCGT GACCCTGGGA TTTTTCAAGA 
     2221 ACCTCAAACT AATCCGGGGA GATTCCATGG TGGATGGGAA CTACACTCTG TACGTGTTAG 
     2281 ACAACCAGAA CCTGCAACAG TTGGGGTCTT GGGTGGCCGC AGGGCTCACC ATTCCCGTGG 
     2341 GCAAAATATA CTTCGCCTTC AACCCACGCC TCTGCCTGGA GCACATATAC CAACTGGAAG 
     2401 AGGTGACCGG AACCAGAGGC CGGCAAAGCA AGGCTGAAAT CAACCCCCGG ACCAATGGAG 
     2461 ATCGTGCTGC CTGCCAGACT CGCACTCTGC GCTTTGTGTT CAACCTGACC GAGGAGGACC 
     2521 GCATCCTCCT GCGCTGGGAG CGCTATGAGC CACTGGAGGC CCGTGACCTG CTCAGCTTCA 
     2581 TCGTCTATTA CAAGGAGTCC CCATTCCAGA ATGCCACAGA ACACGTGGGT CCAGACGCCT 
     2641 GTGGAACTCA GAGCTGGAAT CTGTTGGATG TGGAACTACC CCTCAGCCGC ACCCAGGAGC 
     2701 CCGGGGTGAC CCTAGCCCCC CTAAAGCCCT GGACGCAGTA TGCTGTGTTT GTGCGAGCCA 
     2761 TCACACTGAC CACGGCTGAG GACAGCCCCC ATCAGGGAGC CCAGAGCCCC ATTGTCTACC 
     2821 TGCGAACATT ACCTGCAGCA CCCACAGTAC CCCAGGACGT CATCTCCACC TCCAATTCCT 
     2881 CTTCACACCT CCTGGTGCGC TGGAAGCCGC CGGTCCAGCG CAATGGAAAC ATCACCTATT 
     2941 ACCTGGTGTT GTGGCAGCGG CTAGCAGAGG ACGGTGACCT CTACATCAAT GACTACTGCC 
     3001 ACCGCGGTCT GCGGCTGCCC ACCAGCAGCC ACGACACACG CTTCGACCGT GAAGACCCGG 
     3061 CGCTGGAGGC TGAGCCGGAG CAAGGCTGCT GTCCTTGCCA ACACTCGCCT CCTGGTCAGG 
     3121 CCCTGCCTGC ACTGGAGGCA CAAGAGGTCA CCTTCCAGAA AAAGTTTGAA AACTTCCTAC 
     3181 ACCATGCCAT CACCATCCCC AAGGCCCCGT GGAAAGTGAC ATCCATCAAC AAGAACCCCC 
     3241 AAAGGGATTC GGAGAGGCAC CGCCGGGAAA CTGGGCTTCT CAGACTAGGG AAAAACAACT 
     3301 CGGATTTTGA GATTCATGAG GACAAGGTTC CCCGGGAGCG AGCAGTATTG AGCGGCTTGC 
     3361 GACACTTCAC AGAATACAGG ATTGACATCC ATGCCTGCAA CCACGCGGCA CACACTGTGG 
     3421 GCTGCAGCGC TGCCACCTTC GTCTTTGCAC GCACCATGCC ACATAGAGAG GCCGATGGCA 
     3481 TCCCAGGGAA AGTGGTCTGG AAGGCGGTTG GCAAGAGCAG TGTCATCTTG CATTGGCTTG 
     3541 AGCCACCTGA CCCCAATGGG CTCATCCTCA AGTATGAAAT CAAGTACCGC CGCCTTGGAG 
     3601 AGGAGGCCAC AGTGCTTTGT GTGTCCCGTC TTCGATATGC CAAAGTTGGT GGGGTCCACC 
     3661 TGGCTCTGCT GCCTCCTGGA AACTACTCTG CTAAAGTTCG GGCCACCTCA TTGGCTGGCA 
     3721 ATGGCTCCTG GACTGACGGT GTTGCCTTCT ACATCACCGG TCCAGAGGAA GAGGATACTG 
     3781 GGGGACTGCG CATCCTCCTC ACTGTCACCC CTGTCGGCTT CATGCTGCTC GTGATGCTTG 
     3841 CTGCCCTTGG TTTCTTCTAC AGCAAAAAGA GAAACAGCAC GCTGTACACA TCCGTGAACC 
     3901 CGGAGTATTT TAGTGCATCC CACATGTATG TCCCCGACGA GTGGGAGGTC CCTCGGGAGC 
     3961 AGATAGCCAT CATCCGCGAG CTGGGCCAGG GCTCTTTTGG GATGGTATAT GAGGGACTGG 
     4021 CACGAGGACT TGAGGCTGGA GAGGAGTCTA CACCTGTAGC CCTGAAGACA GTTAATGAGC 
     4081 TGGCCAGCGC TCGGGAACGC GTTGAATTCC TCAAGGAAGC ATCTGTCATG AAGGCCTTCA 
     4141 AGTGTCACCA TGTGGTCCGT CTCCTGGGTG TGGTGTCTCA GGGCCAGCCA ACTTTGGTCA 
     4201 TCATGGAGTT AATGACCCGT GGAGACCTCA AGAGCCATCT CCGATCTCTG AGACCTGAGG 
     4261 CAGAGAACAA CCCTGGCCTC CCACAGCCAG CACTCAGTGA CATGATTCAG ATGGCTGGTG 
     4321 AGATTGCAGA TGGCATGGCC TATCTTGCTG CCAAGAAGTT TGTGCACCGG GACCTGGCAG 
     4381 CCCGCAACTG CATGGTGTCT CAGGATTTTA CCGTCAAAAT TGGTGACTTC GGAATGACTC 
     4441 GGGATGTGTA CGAGACAGAC TATTACCGCA AGGGTGGAAA GGGGCTACTG CCTGTGCGCT 
     4501 GGATGGCCCC TGAGTCCCTC AAAGACGGAA TCTTCACCAC TCACTCAGAC GTCTGGTCCT 
     4561 TTGGTGTGGT GCTCTGGGAG ATTGTGACCC TAGCTGAACA ACCGTACCAG GGCCTATCAA 
     4621 ACGAGCAGGT GCTGAAGTTT GTCATGGACG GTGGAGTTCT AGAGGAGCAA GAGGATTGTC 
     4681 CAATTCAGCT ACAGGAGCTG ATGAGACGCT GCTGGCAGCA TACTCCACGC CTGCGCCCGA 
     4741 CTTTCGTCCA CATCCTGGAT CGCATACAGG ATGAACTTCG TCCCTCTTTC CGGCTCTGCT 
     4801 CCTTCTACTA CAGCCCCGAG TGTCAGCGGG GCCAGGCCTC CTTGCTGCCT ACTGAGGCAG 
     4861 AGCCTGACTC CCCACCAACC TTAAATGGAG CTTCAGACTA TGGTCCCCCA AATGGGGACC 
     4921 CAGGACACAG CGGACCGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4981 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5041 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5101 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5161 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5221 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5281 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5341 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5401 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5461 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5521 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5581 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5641 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5701 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5761 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5821 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5881 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5941 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6001 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6061 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6121 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6181 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6241 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6301 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6361 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6421 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6481 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6541 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6601 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6661 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6721 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6781 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6841 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6901 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6961 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7021 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7081 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7141 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7201 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7261 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7321 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7381 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7441 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7501 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7561 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7621 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7681 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7741 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7801 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7861 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7921 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7981 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8041 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8101 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8161 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8221 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8281 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8341 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8401 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8461 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8521 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8581 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8641 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8701 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8761 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//