LOCUS       RR206357                9231 bp    DNA     circular  SYN 
DEFINITION  Plb1 (Myc-DDK-tagged ORF) - Rat phospholipase B (Phlpb), (10 ug)
ACCESSION   NM_138898
VERSION     NM_138898.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5388..5479 
                     /label="Myc-DDK"  
     misc_feature    5509..6094 
                     /label="PolyA signal"  
     misc_feature    5566..5585 
                     /label="Restriction Enzyme"  
     misc_feature    6370..7042 
                     /label="ColE1"  
     misc_feature    7472..8266 
                     /label="Kan/Neo"  
     misc_feature    8301..8603 
                     /label="SV40 ori"  
     misc_feature    8689..9166 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5370..5376 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5370 
                     /label="Plb1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGTCATGG CCAGGCGTTT CCCTGGTGGG ACTGCTGCTG CTGCTACTGC 
     1081 TGGGACAAGG GCCCTCCCAA ATCCACGGCT CTTCTGGAGA AAACACATCG CAGCCCCAGC 
     1141 AAGTGTTTCG GACCCTGAAG AATTTTTCAT TCCCTTGCAA GCCAAAGAAG TTAGAACTGA 
     1201 GTGTGCTTTC TAAGTCAGTT CACTCTCTGA GACCCTCAGA CATTAAACTC GTGGCAGCCA 
     1261 TCGGCAACCT AGAAACTCCT CCAGCCCCTG GCTCAGGCGT GGTCAACATG GAGAAACCTC 
     1321 AAAGCCTCGA GAGCGAACTA CAGAATGTGT GCATAGGAAT CATGACAGCC CTTTCAGATA 
     1381 TCATCAGACA TTTCAACCCT TCTGTTCTGA TGCCCACGTG TTCTCCTGGG AAGGGTACTG 
     1441 CAGGCCACAC TACTATTGCC GAAGACTTGT GGATTCAGGC TAAAGAGCTG GTGAGGCATC 
     1501 TGAAAGACAA CCCGGAACTT GATTTTGAGA AGGACTGGAA ACTCATCACT GTGCTCTTCA 
     1561 GTAACACAAG CCAGTGCCAC CTGTGTTCCT CTGATCAGCA GAAAAGGCAC TTGATGAAGC 
     1621 ACATGGAGAT GCTGTCAGGG GTGCTGGATT ACCTGCATCG TGAGGTCCCC AGAGCGTTTG 
     1681 TGAATTTGGT GGATCTCTCT GAGGTTTTGA CCATGGCTCA GCAGCATCAA GAGACTGGTT 
     1741 TCAGCCCTGC ACCAGAGATT TGCAAATGCT CAGAGGAAAT AACGAAGTTA TCCAAGGCTG 
     1801 TCATGCAGTG GTCCTATCAG GAAGCCTGGG AAGATCTCCT GGCCTCCAGC AAGTTCAATA 
     1861 AGCATGAGAC CTTCGCTGTG GTTTTCCAGT CTTTCTTCTC TGAGGTAGAA CTACCCTTGG 
     1921 AGAGGCCCTC GCCCCAGGAT TCCACCACGC TCGCCCTCAG GATCTGGAAT AGCATGATGG 
     1981 AACCAGTGGG TCGAAAGGAT GGGACACTCA ATGAAGCAGA GAGAAAAACA ATGAAATGTC 
     2041 CCTCTCAGGA GAGCCCCTAT CTGTTCACCT ACAGAAATAG CAACTACCAG GCCAGACAGC 
     2101 TGAAACCCAT AGGAAAGTTT CAGATGAAAG AAGGAACAAA ATTCACCTGT CCTGACAAAG 
     2161 ACCCCTCGGA CTCCATCCCC ACAACAGTTC ACAGGCTGAG GCCGGCTGAC ATCAAGGTCA 
     2221 TCGGAGCCAT GGGTGACTCG CTCACGGCAG GCAACGGGGC AGGGTCCAGC CCTGGGAATG 
     2281 TCTTGGATGT CTTAACTCAA TACCGAGGCC TGTCGTGGAG TGTGGGCGGA GATGAGACCA 
     2341 TCGAGACCGT GACCACCCTA GCCAACATCC TCCGGGAATT CAACCCCTCC TTGAAGGGCT 
     2401 TCTCTGTTGG CACTGGGAAA GAAAACACTC CCCGAGCATC CTTCAACCAG GCCGTAGCAG 
     2461 GAGCCAAATC TGATGGCTTA GCTGCCCAGG CCAAAAAGCT GGTGAGCCTG ATGAAGGATG 
     2521 ACAAGACAAT AAACTTTCAG GAAGACTGGA AGATAATCAC TGTGTTTATA GGAGGCAACG 
     2581 ACCTCTGTGG CTCCTGCAAT AACCTGGCTC GCTTTTCTCC CCAAACCTTC ACAGACAACA 
     2641 TCAAGACGGC CCTGGACATC CTCCATGCAG AGGTTCCCCG GGCCTTTGTG AACATGGTCT 
     2701 CGGTGATTGA GATCACCCCC TTGAGAGAAC TGTTCAATGA ACCTAAAGTC AGCTGCCCAC 
     2761 GGATGATCCT CAGGAGCCTG TGTCCTTGTG TCTTGAACCT TGGTGAGAAC TCAGCAGAAC 
     2821 TTGCCCAACT TGTGGAAAGG AACAGGCAGT ATCAGGAAGA AACTGGAAAA CTGATTGAGA 
     2881 GTGGCCGATA CGACACCAGG GATGACTTCA CCGTGGTCCT CCAGCCCATG TTTGAAAATG 
     2941 TCGTCATGCC ACGGACCCTG GAGGGCTTGC CCGACAGCTC TTTCTTTGCC CCTGACTGTT 
     3001 TCCACTTCAA TGTCAAGACT CACGCTCGCT CAGCCATCGC CCTCTGGAAG AACATGCTAG 
     3061 AACCTGTGGG CCGCAAGACA AGACATCAGA ATTTTGAAAT CAAGGTCCCT ATCATGTGTC 
     3121 CAAACCAGAC CTCACCGTTT CTGAGCACCA CCAAGAACAG CAACCTGGGA CATGGAACTT 
     3181 CGATGTCTTG TGAGGAGAAA GCCCCCTCTG CCTCACCACC AACCTCAGTG CATACCCTGA 
     3241 GACCTGCAGA CATCCAAGTT GTGGCAGCTC TGGGAGACTC TGTGACTGCT GGCAATGGAA 
     3301 TCAGCTCCCA AGAAGGTGAT CTCGCTGATG TTACCACACA GTATCGAGGA CTGTCCTACA 
     3361 GTGCTGGTGG GGACAAGTTC CTGGAGAATG TGACCACCTT GCCCAACATC CTCCGGGAAT 
     3421 TTAATGGAAA TCTCACAGGC TACTCAGTCG GAACCGGTGA CGTCAACTCT GCAAGCGCGT 
     3481 TCCTTAACCA GGCTGTCCCT GGGGCAAAGG CTGAGAACCT TGCAAGTCAA GTCCAGACTC 
     3541 TGATTCAGAA GATGAAGAAT GACACCAGAG TGAACTTTCA CCAAGACTGG AAGGTCATCA 
     3601 CTGTGATGAT TGGGGCCAGC GACTTGTGTG ACTTCTGCAA GGATTCGAAT CGTTACTCTG 
     3661 CAGCCAATTT TTCTGACCAT CTCCGCAATG CCTTGGACAT CCTGCATAAG GAGGTACCCA 
     3721 GAGCCCTGGT CAACCTTGTG GACTTCATGA ACCCCAGTAT CATTCGGCAA GTGTTCCTGA 
     3781 AGAACCCAGA CAAGTGCCCT GTGAATCAGA CCAGTGTCCT GTGCAACTGT GTTCTGACCC 
     3841 CAGGGGAGGA TTCCCATGAG CTGGCAAGGT TGGAGGCCTT CACCAAATCC TACCAGAGTA 
     3901 GCATGCTTCA ACTGGTTGAG TCAGGCCGCT ACGACACCCG GGAGGATTTC TCTGTGGTAC 
     3961 TGCAGCCCTT TCTCTTCAAC ATCAGGCTCC CCATCCTAGA GAATGGGAAT CCAGATACAT 
     4021 CCTTCTTTGC CCCAGACTGC ATCCTCCTAA GCCAGAAGTT CCACACTCAG CTCGCGAGAG 
     4081 CCCTTTGGGC CAATATGCTT GAACCCCTGG GAAAGAAAAT GGATACTTTG GACCCGAAAG 
     4141 AACTCATAGC TTTGGCCTGC CCCACCAAGG ACAAGCCCTT CCTGAGAACC TTCCGGAACA 
     4201 GTAACTACAC GTACCCTATC AAGCCAGCCA TTGAGAATTG GGGCAGTGAC TTCCTGTGCA 
     4261 CAGAGCAGAG TCCTTCCAGC AAGGTACCCA CCTCAGTTCA TGAGCTCCGA CCATCAGACA 
     4321 TCAAGGTGGT GGCAGCAATG GGTGACTTTC TGACGACAGC CACCGGAGCT CGACCAAGTG 
     4381 AGTCCAGCAG TCTAGACACC CCCTGGAGGG GGCTGTCTTG GAGCATTGGA GGAGATGGAA 
     4441 CGTTGGAGAC CCATACCACA CTGCCCAACA TCCTGAAGAA GTTCAACCCT TCCATCCTTG 
     4501 GATTCTCCAC CGGTACCCTG GAGAACACGG CAGGATTAAA TGTGGCAGAA GAAGGCGCCA 
     4561 GAGCTCAGGA CATGCCGGCC CAGGCTCAGG CCCTGGTGAA GAAGATGAAA AGCACCCCTA 
     4621 CAATCAACAT ACAGGAAGAC TGGAAGCTGA TTACACTCCT CATCGGGAAC AACGACCTGT 
     4681 GTCTTTACTG TGAGGATCCG GAGAACTACT CAACCAGGGA GTATGTCAAG TACATCCAGC 
     4741 ATGCCTTGGA CATCTTCTAT GAGGAGCTTC CCAGGGTTTT CATCAACGTG GTGGAAGTCA 
     4801 TGGAGCTGTC CGGTCTGCTC CACGACCAGG GCGGGAAATG TGCCATGCCG TTGGCTGTCC 
     4861 AGAAAAACTG CAGTTGCCTT AAACGCTCTC AAAACCTCAT GGCAATGCAG GAGCTGAAGA 
     4921 AAGTCAACGG GAACCTCCAG AGTGCCCTCT CGGAGCTCTC CTACTGGCAT CGGTACATGC 
     4981 AGCGTGAGGA CTTTGCAGTC ACTGTCCAGC CTTTCTTCCG GAATACCTTT GTCCCACTGG 
     5041 ATGAGCGTGG GGGCCTCGAC CTCACTTTCT TCTCTGAAGA CTGTTTCCAC TTCTCAGTCC 
     5101 GTGGGCATGC TGAGATGGCC ATTGCCCTCT GGAACAACAT GCTGGAACCA GTGGGCAAGA 
     5161 AGACAACCTC CAATAACTTC ACATACAACC GGACCAAACT CAAGTGTCCC TCGCCTGAAA 
     5221 ACCCTTTCCT CTACACTGTC CGGAACAGTC AGATTCTTCT AGACAAGGCT AAAGAAAACT 
     5281 CCAATACACT CTACTGGGCA GTGCCAGTGG CTGCAGTAGG TGGCCTGGTA GTTGGCATCC 
     5341 TTGGAATGAT GTTGTGGAGA ACTGTGAGAC TCGTCCAAAC GCGTACGCGG CCGCTCGAGC 
     5401 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5461 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5521 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5581 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5641 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5701 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5761 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5821 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5881 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5941 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     6001 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     6061 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     6121 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     6181 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     6241 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     6301 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     6361 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     6421 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6481 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6541 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6601 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6661 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6721 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6781 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6841 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6901 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6961 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     7021 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     7081 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     7141 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     7201 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     7261 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     7321 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     7381 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     7441 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7501 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7561 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7621 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7681 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7741 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7801 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7861 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7921 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7981 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     8041 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     8101 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     8161 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     8221 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     8281 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     8341 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     8401 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     8461 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8521 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8581 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8641 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8701 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8761 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8821 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8881 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8941 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     9001 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     9061 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     9121 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     9181 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//