LOCUS       RR206302                7938 bp    DNA     circular  SYN 
DEFINITION  Fuk (Myc-DDK-tagged ORF) - Rat fucokinase (Fuk), (10 ug)
ACCESSION   NM_001107429
VERSION     NM_001107429.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4095..4186 
                     /label="Myc-DDK"  
     misc_feature    4216..4801 
                     /label="PolyA signal"  
     misc_feature    4273..4292 
                     /label="Restriction Enzyme"  
     misc_feature    5077..5749 
                     /label="ColE1"  
     misc_feature    6179..6973 
                     /label="Kan/Neo"  
     misc_feature    7008..7310 
                     /label="SV40 ori"  
     misc_feature    7396..7873 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4077..4083 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4077 
                     /label="Fuk"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACCAGCCA AAGGGGGTCA ATTGGACGGT CATTATCCTG ACATGCCAGT 
     1081 ACAAGGACAG TGTCCAGGTC TTTCAGAGAG AGCTGGAGGT AAGGCAGAAG CGGGAGCAGA 
     1141 TCCCTGCCGG GACGATGTTA CTGGCTGTGG AGGACCCCCA GACCCGAGTA GGCAGTGGAG 
     1201 GAGCTACTCT CAATGCACTG CTGGTGGCTG CTGAGCACCT GAGTGCCCGA GCTGGCTTCA 
     1261 CCGTGGTCAC GTCAGATGTC CTGCACTCGG CTTGGATTCT CATCTTGCAC ATGGGCCGAG 
     1321 ACTTCCCCTT TGATGACTGT GGCAGGGCCT TCACTTGCCT CCCTGTGGAG AATCCACAGG 
     1381 CCCCTGTGGA GGCCTTGGTA TGCAACCTGG ACTGCCTGTT GGATATCATG ACCCACCGGC 
     1441 TGGGTCCAGG ATCCCCACCA GGTGTGTGGG TCTGCAGCAC CGACATGCTT CTGTCTGTTC 
     1501 CTCCAAACCC TGGGATCAGT TGGGATGGCT TCCGGGGAAC CAGAGTGATC GCCTTTCCTG 
     1561 GGAGCCTGGC CTACGCTCTA AACCACGGGG TCTACCTCAC TGACTCGCAG GGCGTGGTTT 
     1621 TGGACATTTA CTACCAGGGC ACTAAGGCAG AGATACAACG GTGTGTCAGG CCTGATGGAC 
     1681 TGGTACCACT GGTCTCTGGG GTTGTCTTCT TCTCTGTGGA GACTGCTGAG CACCTCCTAG 
     1741 CCACCCACGT GAGCCCACCG CTGGACGCCT GCACCTATAT GGGCTTGGAC TCTGGAGCCC 
     1801 AGCCTGTGCA GCTGTCTCTG TTTTTCGACA TCCTGCTCTG CATGGCTCGG AATATGAGCA 
     1861 GGGAGAACTT CGTGGCTGGG CGGCCCCCGG AGATGGGGCA AGGTGACCCG GATGTAGCAC 
     1921 GTTACCTGAA GGGAGCCCGG GCCCAGCTGT GGAGGGAGCT TCGAGATCAG CCCCTCACTA 
     1981 TGGTGTATGT CCCTGATGGC GGTTACAGTT ACATGACAAC TGATGCCACG GAGTTCCTGC 
     2041 ACAGACTCAC GATGCCTGGA GTAGCTGTGG CCCAGATTGT TCACTCTCAG GTGGAGGAGC 
     2101 CACAGCTGCT AGAGGCTACG TGCTCCGTGG TCAGCTGCCT GCTGGAGGGT CCCGTGCACC 
     2161 TGGGGCCTCG AAGTGTCCTG CAGCACTGTC ACCTGAGGGG CCCCATTCAT ATTGGCGCTG 
     2221 GCTGCTTTGT GAGTGGCCTG GATACCGCCC ACTCCGAGGC ACTGCATGGC CTGGAGCTTC 
     2281 ATGACCTCAT CCTTCAGGGA CACCACATAC GGCTGCATGG CTCCCAGAGT CGTGTATTCA 
     2341 CTCTTGCTGG CCGTCTGGAC AGCTGGGAAA GACAGGGGGC AGGCATGTAT CTCAACATGT 
     2401 CCTGGAATGA GTTCTTCAAG AAGACAGGCA TTCGAGACTG GGACCTGTGG GACCCAGATA 
     2461 CACCCCTCTC AGATCGATGC CTTCTCAGTG CCCGCCTTTT CCCTGTGCTC CACCCCACGA 
     2521 GGGCTCTGGG GCCCCAGGAT GTGCTGTGGA TGCTGCATCC TCATAAGGAC AGAGGCGAGG 
     2581 CCCTGCGTGC CTGGAGAGCC TCCTGGCGTC TGTCCTGGGA GCAGCTGCAA CCTCGCCTGG 
     2641 ACCGGGCTGC CACACTGGAC TTCCGTCGGG ATCTGTTCTT CCGCCAGGCC TTGCAGAAGG 
     2701 CGAGGCATGT GTTAGAGGCC CGGCAGGACC TCTGCCTACA TCCACTGATC CGGGCTGCTG 
     2761 TCGGTGAAGG TTGCTCTGGG CCCCTGCTGG CCACACTTGA CAAGGTTGCA GCAGGGGCAG 
     2821 AAGATCCTGG TGTGGCAGCC CGGGCTCTGG CTTGTGTGGC AGATGTACTC GGCTGCATGG 
     2881 CAGAGGGCCA AGGAGGCTTG CGCAGTGGGC CAGCTGCCAA CCCTGAGTGG ATTCAGCCTT 
     2941 TCTCATACTT GGAACGTGGA GACCTCATGA GGGGTGTGGA GGCACTTGCC CAGGAAAGAG 
     3001 AGAAGTGGCT GACCAGGCCT GCCTTGTTGG TTCGAGCTGC CCGCCATTAT GAGGGGGCTG 
     3061 AGCAGATCCT GATCCGACAG GCTGTGATGA CAGCCCGGCA CTTCGTCTCC ACCCAGCCAG 
     3121 TGGAATTGCC AGCACCTGGG CAGTGGGTGG TGACTGAGTG CCCAGCCCGT GTGGATTTCT 
     3181 CTGGGGGCTG GAGTGACACA CCACCCATTG CCTATGAGCT TGGTGGAGCA GTATTGGGCC 
     3241 TGGCTGTTCG GGTGGATGGC CGCCGGCCCA TCGGGGCCAA GGCACGCCGC ATCCTAGAGC 
     3301 CTGAGCTCTG GCTGGCAGTG GGACCTCGAC AGGATGAGAT GACCGTGAAG ATAGTGTGCC 
     3361 GGAGCCTTGA TGACCTGCAG GATTACTGCC AGCCTCATGC CCCAGGTGCC TTGCTGAAGG 
     3421 CAGCCTTTAT CTGTGCGGAT ATTGTGCATG TCAACTCAGA GGTCCCTCTG CATGAACAGT 
     3481 TGCTACGCTC GTTTAATGGT GGCTTTGAGC TGCACACATG GTCAGAGCTG CCACACGGCT 
     3541 CTGGTCTTGG CACTAGCAGC ATCTTGGCAG GGGCTGCCCT GGCTGCTTTG CAGCGGGCTG 
     3601 CAGGCCGGAC AGTGGGCACA GAGGCTCTCA TCCATGCAGT GTTGCACCTG GAGCAGGTGC 
     3661 TCACCACAGG AGGTGGCTGG CAGGACCAAG TGAGTGGCCT AATGCCTGGC ATCAAGGTGG 
     3721 GGCGCTCTCG GGCACAGCTG CCCCTAAAGG TGGAGGTGGA GGAAATCACT GTGCCTGAGA 
     3781 ACTTTGTCCA GAGGAAGCTT ATGGCCCCAG GCTGTGAGCC GCTGGCTGTG CATCGGATGA 
     3841 TGGATGTCCT GGCCCCTTAT GCCTTCGGCC AAAGTCTGGC AGGGGCAGGC GGTGGGGGCT 
     3901 TTCTCTATCT GTTGACCAAG GAACCCCGGC AGAAAGAGGT CCTAGAAGCT GTGCTGGCCA 
     3961 AGGTGGAGGG CCTCGGCAAC TACAGCGTCC ACCTGGTGCA AGTGGACACT CAGGGCCTGA 
     4021 GCCTGCAGCT GCTAGGACAT GACGCCCATC TTTGCGGGGC TGGGCCCTCT GAAGTGGGCA 
     4081 ACACCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4141 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4201 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4261 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4321 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4381 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4441 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4501 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4561 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4621 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4681 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4741 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4801 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4861 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4921 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4981 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5041 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5101 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5161 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5221 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5281 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5341 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5401 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5461 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5521 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5581 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5641 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5701 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5761 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5821 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5881 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5941 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6001 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6061 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6121 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6181 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6241 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6301 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6361 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6421 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6481 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6541 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6601 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6661 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6721 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6781 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6841 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6901 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6961 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7021 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7081 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7141 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7201 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7261 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7321 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7381 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7441 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7501 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7561 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7621 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7681 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7741 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7801 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7861 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7921 AAATTTAACG CGAATTTT
//