LOCUS       RR206288                8394 bp    DNA     circular  SYN 
DEFINITION  Glg1 (Myc-DDK-tagged ORF) - Rat golgi apparatus protein 1 (Glg1), (10 ug)
ACCESSION   NM_017211
VERSION     NM_017211.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4551..4642 
                     /label="Myc-DDK"  
     misc_feature    4672..5257 
                     /label="PolyA signal"  
     misc_feature    4729..4748 
                     /label="Restriction Enzyme"  
     misc_feature    5533..6205 
                     /label="ColE1"  
     misc_feature    6635..7429 
                     /label="Kan/Neo"  
     misc_feature    7464..7766 
                     /label="SV40 ori"  
     misc_feature    7852..8329 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4533..4539 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4533 
                     /label="Glg1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGTGTGT GGACGTGTAC GGAGGATGTT CCGCTTGTCG GCGGCGTTGC 
     1081 AGCTGCTGCT GCTAGTGGCA GCAGGGGTCC AGAATAGCCA CGGTCAGGGC CAGGGTCTGG 
     1141 GGGTCAACTT TGGGCCCTTC GCAGGGCAAG CTGGAGGCGG CAACCCGGTC GGGCAGCAGC 
     1201 CACCTCAGCT GCCTCAGTTA TCGCAGCAGC AGCAGCAGCA GCAGCCGCCG CCTCAGCAGC 
     1261 AGCAGCCTTT CCCGGCGGGC GGGCTCCCGG CCCGGCGGGG CGGAGCAGGG CCCGGTGGGA 
     1321 CTGGCGGAGG CTGGAAGCTG GCGGAGGAAG AGTCCTGCAG AGAGGACGTG ACCCGTGTGT 
     1381 GCCCCAAACA CACCTGGAGC AACAACCTGG CGGTGCTCGA GTGCCTGCAG GACGTGAGGG 
     1441 AGCCTGAGAA TGAGATTTCT TCAGACTGCA ATCATTTGTT GTGGAATTAC AAGCTGAACC 
     1501 TGACTACTGA TCCCAAATTT GAATCTGTGG CCAGGGAAGT TTGCAAGTCA ACTATTTCAG 
     1561 AGATTAAAGA GTGTGCTGAA GAACCAGTTG GAAAAGGTTA CATGGTTTCC TGCTTGGTGG 
     1621 ATCACCGAGG CAACATCACT GAATACCAGT GTCATCAATA CATTACCAAA ATGACAGCCA 
     1681 TCATTTTTAG TGATTATCGT TTGATCTGTG GCTTCATGGA TGACTGCAAA AATGACATCA 
     1741 ACCTCTTGAA ATGTGGCAGC ATTCGCCTTG GGGAAAAGGA TGCACATTCA CAAGGTGAGG 
     1801 TGGTATCATG TTTGGAAAAA GGCCTGGTGA AAGAGGCAGA AGAGAAAGAG CCCAAGATTC 
     1861 AAGTCTCAGA ACTCTGCAAG AAAGCCATTC TTAGGGTGGC TGAGCTGTCC TCGGATGACT 
     1921 TTCATTTAGA CCGGCATTTG TATTTTGCTT GCAGAGATGA TCGGGAGCGC TTTTGTGAGA 
     1981 ATACACAAGC TGGTGAAGGA AGAGTGTATA AATGCCTTTT TAACCATAAA TTTGAAGAAT 
     2041 CCATGAGTGA AAAGTGTAGA GAAGCACTAA CTACACGGCA GAAGCTCATT GCCCAGGATT 
     2101 ATAAAGTCAG CTATTCATTG GCCAAGTCCT GTAAGAGTGA CTTGAAAAAA TACCGATGTA 
     2161 ATGTGGAAAA CCTTCCTCGC TCCCGAGAGG CCAGGCTCTC CTACCTTCTG ATGTGCCTGG 
     2221 AGTCAGCCGT GCACAGAGGG CGACAAGTGA GCAGTGAGTG CCAAGGTGAG ATGCTGGATT 
     2281 ACCGACGCAT GCTGATGGAA GACTTCTCTC TGAGCCCCGA GATCATCCTG AGCTGTCGAG 
     2341 GGGAGATCGA ACACCACTGT TCTGGATTAC ATCGGAAAGG ACGCACCCTC CACTGTCTGA 
     2401 TGAAAGTAGT CCGGGGTGAA AAGGGGAGCC TTGGAATGAA CTGCCAGCAG GCGCTTCAGA 
     2461 CACTGATTCA GGAGACTGAC CCTGGCGCAG ACTACCGCAT TGATCGAGCT TTGAATGAAG 
     2521 CTTGTGAATC TGTAATACAG ACAGCCTGCA AACATATACG ATCCGGAGAC CCAATGATTC 
     2581 TTTCATGTCT GATGGAACAT TTATACACAG AGAAAATGGT GGAAGACTGT GAACACCGTC 
     2641 TCTTAGAGCT ACAGTATTTT ATCTCTCGGG ATTGGAAGTT GGACCCTGTT TTATACCGGA 
     2701 AATGCCAGGG AGATGCTTCC CGTCTTTGCC ACACCCATGG TTGGAATGAG ACCAGTGAAC 
     2761 TTATGCCTCC CGGAGCTGTG TTTTCTTGCC TGTATAGACA TGCCTACCGC ACAGAAGAGC 
     2821 AAGGACGGAG GCTCTCACGA GAATGCCGAG CTGAAGTCCA GAGGATCCTA CACCAGCGTG 
     2881 CCATGGATGT TAAGCTGGAT CCTGCCCTCC AGGACAAGTG CCTGATAGAT CTAGGAAAGT 
     2941 GGTGCAGTGA GAAGACAGAG ACTGGTCAGG AGCTTGAGTG CCTTCAGGAC CATCTGGATG 
     3001 ACTTAGCTGT GGAATGCAGA GACATAGTGG GCAACCTCAC GGAGTTAGAG TCTGAGGACA 
     3061 TACAAATAGA AGCTTTGCTG ATGAGAGCCT GTGAGCCTAT AATTCACAAC TTTTGCCACG 
     3121 ATGTGGCAGA CAACCAGATT GACTCTGGGG ACCTAATGGA GTGTCTGATC CAGAACAAGC 
     3181 ACCAGAAGGA CATGAATGAG AAGTGCGCCA TTGGCGTCAC TCACTTCCAG CTGGTCCAAA 
     3241 TGAAGGATTT TCGATTCTCT TACAAGTTCA AAATGGCCTG CAAGGAGGAT GTGTTAAAAC 
     3301 TTTGCCCCAA CATAAAAAAG AAGGTGGATG TGGTGATCTG CCTGAGCACC ACCGTGCGCA 
     3361 ACGACACTCT GCAGGAGGCC AAGGAGCACC GGGTGTCTCT CAAGTGCCGC AAGCAGCTCC 
     3421 GTGTGGAGGA GCTGGAGATG ACAGAGGACA TCCGTTTGGA ACCAGATCTG TATGAAGCCT 
     3481 GCAAGAGTGA CATCAAGAAC TACTGTTCTA CAGTGCAATA TGGAAATGCT CAGATTATTG 
     3541 AATGTCTAAA AGAAAACAAG AAGCAACTGA GTACCCGTTG TCACCAGAGA GTATTTAAGC 
     3601 TGCAGGAGAC AGAGATGATG GACCCAGAAC TAGACTATAC ACTCATGAGA GTCTGCAAGC 
     3661 AGATGATTAA GCGGTTCTGT CCAGAAGCAG ATTCTAAAAC TATGTTACAG TGTTTGAAAC 
     3721 AAAACAAGAA CAGTGAATTG ATGGACCCCA AGTGCAAACA GATGATCACC AAGCGTCAGA 
     3781 TCACCCAGAA CACAGATTAC CGTTTAAACC CCGTGCTAAG GAAGGCCTGT AAAGCTGACA 
     3841 TTCCTAAATT CTGTCATGGC ATCCTGACCA AGGCCAAGGA TGATTCAGAG CTAGAAGGCC 
     3901 AAGTTATCTC ATGCCTCAAG CTGAGATACG CTGACCAGCG CCTGTCTTCA GACTGTGAGG 
     3961 ATCAGATCCG TATCATCACC CAAGAGTCTG CGCTAGATTA CCGCCTAGAC CCTCAGCTCC 
     4021 AGCTGCACTG CTCAGACGAG ATTGCTAATT TATGTGCTGA AGAAGCAGCA GCCCAGGAAC 
     4081 AGACAGGCCA AGTGGAAGAG TGCCTAAAGG TCAACCTGCT CAAGATCAGG ACAGAGCTGT 
     4141 GTAAAAAGGA AGTGTTGAAC ATGTTGAAGG AAAGCAAAGC AGACATCTTT GTGGATCCAG 
     4201 TTCTTCACAC AGCGTGTGCC TTGGACATTA AACACCACTG TGCAGCCATC ACCCCCGGCC 
     4261 GTGGGCGTCA GATGTCCTGC CTGATGGAGG CCTTGGAAGA CAAGCGCGTG AGGTTGCAGC 
     4321 CCGAGTGCAA AAAGCGCCTG AATGACCGAA TCGAGATGTG GAGTTATGCA GCCAAGGTGG 
     4381 CCCCAGCGGA CGGCTTCTCT GATCTTGCCA TGCAAGTGAT GACATCCCCA TCAAAGAATT 
     4441 ACATCCTGTC TGTGATCAGC GGGAGCATCT GCATATTGTT CCTGATTGGC CTGATGTGTG 
     4501 GACGGATCAC TAAGAGGGTG ACACGAGAGC TGAAGGACAG GACGCGTACG CGGCCGCTCG 
     4561 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4621 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4681 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4741 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4801 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4861 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4921 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4981 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5041 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5101 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5161 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5221 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5281 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5341 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5401 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5461 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5521 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5581 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5641 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5701 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5761 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5821 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5881 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5941 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6001 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6061 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6121 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6181 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6241 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6301 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6361 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6421 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6481 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6541 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6601 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6661 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6721 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6781 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6841 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6901 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6961 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7021 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7081 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7141 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7201 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7261 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7321 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7381 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7441 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7501 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7561 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7621 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7681 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7741 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7801 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7861 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7921 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7981 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8041 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8101 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8161 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8221 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8281 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8341 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//