LOCUS       RR206274                6325 bp    DNA     circular  SYN 
DEFINITION  Tnfrsf1b (Myc-DDK-tagged ORF) - Rat tumor necrosis factor receptor superfamily, member 1b (Tnfrsf1b), (10 ug)
ACCESSION   NM_130426
VERSION     NM_130426.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2482..2573 
                     /label="Myc-DDK"  
     misc_feature    2603..3188 
                     /label="PolyA signal"  
     misc_feature    2660..2679 
                     /label="Restriction Enzyme"  
     misc_feature    3464..4136 
                     /label="ColE1"  
     misc_feature    4566..5360 
                     /label="Kan/Neo"  
     misc_feature    5395..5697 
                     /label="SV40 ori"  
     misc_feature    5783..6260 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2464..2470 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2464 
                     /label="Tnfrsf1b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCCCGCC GCCCTCTGGG TCGCGCTGGT CGTCGAACTG CAGCTGTGGG 
     1081 CCACCGGGCA CACAGTGCCC GCCAAGGTTG TCTTGACACC CTACAAGCCA GAACCTGGGA 
     1141 ACCAGTGCCA GATCTCACAG GAGTACTATG ACAAGAAGGC TCAGATGTGC TGTGCTAAGT 
     1201 GTCCCCCTGG CCAGTATGCA AAACACTTCT GCAACAAGAC TTCAGACACC GTGTGTGCGG 
     1261 ACTGTGCGGC AGGCATGTTT ACCCAGGTCT GGAACCATCT GCATACATGC CTGAGCTGCA 
     1321 GTTCTTCCTG TAGTGATGAC CAGGTGGAGA CCCACAACTG CACTAAAAAA CAGAACCGAG 
     1381 TGTGTGCTTG CAACGCTGAC AGTTACTGTG CCTTGAAATT GCATTCTGGG AACTGTCGAC 
     1441 AGTGCATGAA GCTGAGCAAG TGTGGCCCTG GCTTCGGAGT GGCCCGTTCA AGAACCTCAA 
     1501 ATGGAAACGT GATATGCAGT GCCTGTGCCC CAGGGACGTT CTCTGACACC ACATCATCCA 
     1561 CAGATGTGTG CAGGCCCCAC CGCATTTGTA GCATCCTGGC TATTCCTGGA AATGCAAGCA 
     1621 CAGATGCAGT CTGTGCATCC GAGTCCCCAA CTCCAAGCGC TGTTCCAAGG ACAATCTACG 
     1681 TATCTCAGCC AGAGCCCACA AGATCCCAGC CCATGGATCA AGAGCCAGGG CCTAGCCAAA 
     1741 CTCCACACAT CCCTGTGTCC TTGGGTTCAA CCCCCATCAT TGAACCAAGC ATCACGGGTG 
     1801 GCATCTCTCT TCCAATTGGT CTGATCGTTG GACTGACAAC TCTGGGTCTG CTGATGTTAG 
     1861 GACTGGCGAA CTGCTTCATC CTGGTGCAGA GGAAAAAGAA GCCCTCCTGC CTACAACGAG 
     1921 AAACCATGGT GCCTCATCTG CCTGATGACA AATCCCAGGA TGCAATAGGC CTTGAACAGC 
     1981 AGCACCTATT GACCACAGCA CCCAGTTCCA GCAGCAGCTC CCTGGAGAGC TCAGCCAGCG 
     2041 CTGGGGACAG GAGAGCGCCC CCTGGGGGTC ATCCCCAAGC AAGAGTCACA GCGGAGGCCC 
     2101 AAGGGTCTCA GGAAGCCTGT GCCGGCTCCA GGAGTTCAGA TTCTTCCCAT GGCAGCCACG 
     2161 GGACCCATGT CAACGTCACC TGCATCGTGA ACGTCTGTAG CAGCTCTGAC CACAGCTCTC 
     2221 AGTGTTCTTC CCAAGCCAGC ACCACGGTGG GAGACCCAGA TGCTAACCCT TCAGGGTCTC 
     2281 CAAAGGATGA GCAGGTCCCC TTTTCCCAGG AGGAGTGTCC CTCTCAGTCC CAGTGGGAGA 
     2341 CCACAGAGAC ACTGCAGAAC CATGACAAGC CCTTTCCCCT TGGTGTGCCT GATGTGGGTA 
     2401 TGAAGCCCAA CCAACCAGGC TGGTATGACC AGATTGCTGT CAAAGTGCCT CCAGATTGCT 
     2461 GTCAAAGTGC CTACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG 
     2521 GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC 
     2581 GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC 
     2641 TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG 
     2701 TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT 
     2761 ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC 
     2821 AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC 
     2881 GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG 
     2941 CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA 
     3001 CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG 
     3061 AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT 
     3121 CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT 
     3181 TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG 
     3241 GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 
     3301 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 
     3361 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC 
     3421 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC 
     3481 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG 
     3541 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 
     3601 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 
     3661 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC 
     3721 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG 
     3781 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG 
     3841 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 
     3901 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG 
     3961 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT 
     4021 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA 
     4081 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG 
     4141 GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG 
     4201 GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA 
     4261 CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG 
     4321 TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC 
     4381 GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT 
     4441 GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG 
     4501 CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA 
     4561 ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA 
     4621 ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC 
     4681 TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG 
     4741 GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC 
     4801 ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA 
     4861 CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC 
     4921 GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA 
     4981 GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC 
     5041 GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA 
     5101 GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC 
     5161 CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT 
     5221 CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA 
     5281 GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA 
     5341 ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC 
     5401 GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG 
     5461 AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG 
     5521 AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG 
     5581 TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT 
     5641 TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG 
     5701 AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC 
     5761 AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG 
     5821 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG 
     5881 AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC 
     5941 GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC 
     6001 TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT 
     6061 AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG 
     6121 TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC 
     6181 GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA 
     6241 TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT 
     6301 TTAACAAAAA TTTAACGCGA ATTTT
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