LOCUS       RR206263                9249 bp    DNA     circular  SYN 
DEFINITION  Depdc5 (Myc-DDK-tagged ORF) - Rat DEP domain containing 5 (Depdc5), (10 ug)
ACCESSION   NM_001107229
VERSION     NM_001107229.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5406..5497 
                     /label="Myc-DDK"  
     misc_feature    5527..6112 
                     /label="PolyA signal"  
     misc_feature    5584..5603 
                     /label="Restriction Enzyme"  
     misc_feature    6388..7060 
                     /label="ColE1"  
     misc_feature    7490..8284 
                     /label="Kan/Neo"  
     misc_feature    8319..8621 
                     /label="SV40 ori"  
     misc_feature    8707..9184 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5388..5394 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5388 
                     /label="Depdc5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGCGACTA AAGAAAAGTT TGGTGAGCAC CTGTGCCTAT ATCACCCAGA 
     1081 AAGTGGAATT TGCTGGCATC AGGGCACAGG CTGGTGAGCT GTGGGTCAAG AATGAGAAGG 
     1141 TCATGTGTGG TTACATTAGT GAAGAGACCA GGGTGGTGTT CCGTTCTACG TCGGCTATGG 
     1201 TTTACATATT TATTCAGATG AGCTGTGAAA TGTGGGATTT TGATATTTAT GGGGATCTGT 
     1261 ACTTTGAGAA AGCTGTGAAT GGTTTCCTTG CCGACCTGTT TACTAAGTGG AAGGAGAAGA 
     1321 ACTGTAGCCA TGAAGTGACT GTGGTTCTGT TTTCTAGAAC TTTTTATGAT GCAAAATCTA 
     1381 TTGATGAATT TCCTGAAGTA AACCGAGCTT CAATTCAACA GGATCATAAA GGGAGATTCT 
     1441 ACGAGGACTT TTACAAAGTG GTGGTGCAGA ACGAGAGAAG GGAAGAGTGG ACGTCACTCC 
     1501 TTGTGACCAT TAAGAAACTC TTCATCCAGT ATCCAGTGTT GGTTCGACTG GAACAGGCAG 
     1561 GGGGCTTTCC TCAAGGAGAC AATTCTACCT CAGCACAAGG AAACTACCTA GAGGCCATCA 
     1621 ACTTGTCATT CAATGTGTTT GACAAGCACT ACATCAACCG AAACTTTGAC CGAACTGGGC 
     1681 AGATGTCCGT GGTGATCACG CCGGGGGTGG GTGTCTTTGA AGTGGACCGC CTCCTCATGA 
     1741 TCTTGACCAA GCAGCGGATG ATCGATAATG GAATTGGTGT GGACTTAGTG TGCATGGGAG 
     1801 AGCAGCCATT ACACGCTGTG CCATTATTCA AGCTGCACAA CCGGAGTGTG CCACGGGATT 
     1861 CTCGGCTGGG CGATGACTAT AATATTCCTC ACTGGATAAA CCACAGTTTC TACACATCCA 
     1921 AAAGCCAGCT GTTTTGTAAC AGTTTCACTC CAAGGATAAA ACTGGCAGGA AAGAAGTCTG 
     1981 CCACTGAGAA AACCAAAAAT GGCCGTGATA CATCTCTTGG GGCTCCAAAG GAATCTGAAA 
     2041 ACACCCTTCC CATCCAAGTA GATTATGATG CCTACGATGC CCAAGTATTC CGGCTGCCTG 
     2101 GTCCATCCCG GGCCCAGCGA CTCGCCACCT GCAGGTCTGT GAGAGAGCAG GAAAATCACA 
     2161 ATCGCAAAAG TGCCAGCTCC TGTGACGTCT CCTCCAGCCC TTCCTTGCCA AGCCGTGCAC 
     2221 TGCCCACTGA GGAAGTGAGG AGCCAGGCTT CAGACGACAG CTCCCTGGGC AAGAGCACCA 
     2281 ACATCCTGAT GATCCCTAAC CCCCATCTGC ACCAGTATGA AGTCAGCAGC TCACTGGGCT 
     2341 ACACCAGCAC CCGAGATGTC CTGGAGAACA TGATAGAACC ACCACAGCGG GACTCTAGTG 
     2401 CACCTGGAAG GTTCCACGTG GGCAGTGCAG AGTCCATGCT ACATGTTCGA CCTGGAGGCT 
     2461 ACACCCCTCA GAGAGCACTG ATTAACCCCT TCGCCCCCTC AAGAATGCCC ATGAAGCTCA 
     2521 CCTCCAACAG AAGGCGCTGG ATGCATACTT TTCCTGTAGG ACCATCTGGA GAGGCCATCC 
     2581 AGATCCATCA TCAAACCCGG CAAAACATGG CAGAGCTGCA GGGCAGCAGG CAGCGGGACC 
     2641 CCACCCACTC CTCTGCAGAA CTGTTGGAGC TGGCATATCA TGAAGCCGCA GGAAGGCACA 
     2701 GCACTTCCCG ACAGCCTGGT GACAGCATGT CCTTGAACTT CAGTGGAACG GAAGAGCTTT 
     2761 CTGTCAGCCT GCTTAGCAAC AGCGGCACAG GTATGAATCC TAGGAACCAG AATAAGGACT 
     2821 CTCTAGAGGA TGGTGTTTCT ACCTCTCCAG ACCCAATGCC AGGCTTCTGT TGCACAGTTG 
     2881 GAGTGGATTG GAAGTCTCTT ACTACTCCTG CATGCCTTCC CCTCACCACT GACTACTTCC 
     2941 CGGACCGTCA GGGCCTGCAG AATGACTACA CAGAGGGCTG CTATGATCTC CTCCCAGAAG 
     3001 CAGACATGGA CAGGAGGGAC GAGGAGGGCG TTCAAATGAC AGCCCAGCAA GTGTTTGAAG 
     3061 AGTTCATCTG CCAGCGTCTC ATGCAGGGTT ACCAAATCAT AGTACAGCCA AAGACCCAGA 
     3121 AGCCCAGCAC TACAGTCCCA CCCCCGCTGA GCAGTAGCCC ACTCTATAGC AGAGGCCTTG 
     3181 TGTCCCGAAA CCGCCCTGAA GAGGAGGGCC AGTATTGGCT GAGTATGGGC AGAACCTTCC 
     3241 ATAAGGTGAC ACTTAAGGAC AAGATGATCA CAGTGACTCG TTACCTTCCC AAGTACCCTT 
     3301 ATGAATCTGC CCAGATCCAT TACACCTACA GCCTCTGCCC TTCCCACTCT GACTCAGAGT 
     3361 TTGTCTCCTG TTGGGTGGAA TTCTGCCATG AACGGCTAGA GGAATACAAG TGGAATTACT 
     3421 TAGATCAGTA CATTTGTTCT GCCGGCTCCG AAGATTTCAG CTTAATCGAG TCTCTGAAGT 
     3481 TCTGGAGAAC CCGATTTCTA CTACTGCCAG CCTGTGTCAC TGCCACCAAG CGCATCACAG 
     3541 AAGGGGAAGT GCACTGTGAC ATCTACGGGG ACAAACCCCG TGCAGATGAA GATGAGTGGC 
     3601 AGCTTCTAGA TGGCTTTATT CGCTTTGTAG AGGGCTTGAA CCGCATCCGC AGGCGCCACC 
     3661 GGTCAGACCG CATGATTCGG AAAGGGACTG CCATGAAAGG CTTGCAGATG ACCGGGCCCA 
     3721 TCTCCGCACA CTCTCTCGAG TCAACGGGCC CCCCAGTCGG GAAGAAAGGA ACCTCAGCTC 
     3781 TGTCTGCCCT GCTGGAGATG GAGGCTAGTC AGAAGAGCCT AGGAGAACAG CAGACAAGTG 
     3841 TGCATGGGAA AAGCTCTACT CAGCCAGCTG AGAACAGCAG TGTTGCCATG ACTCCCACCT 
     3901 ATGTGGACAG CCCACGCAAG GCATCTGTGG ACCAAACAGC TCCTCTGGTG TTGGACAGCA 
     3961 CCAGTTTAGG GGTCAGCACG TGCCAGTCCG TGGATAGAGG CAATAACCAG ACCTTTGGGA 
     4021 ACTCCCAGAA CATAGAACAG GCCTTTCCCT CTACAGGCTC TGGAGACGGC AGCTCTCAGC 
     4081 AGCACATAGC CAGCTCTCTG ACATCGTCCT CTACCCTGGT GGAGATCCTG GAAGCCATGA 
     4141 AGCATCCCTC GACAGGAGTC CAGCTGCTGT CTGAGCAGAA GGGCCTTTCG CCGTGCTGCT 
     4201 TCATCAGTGC GGAGGTCGTA CACTGGCTGA TGAACAATGT GGAGGGGGTC CAGACGCAGG 
     4261 CGATGGGCAT CGACATCATG CAGAAAATGC TGGAAGAGCA GCTCATAGCG CATGCATCTG 
     4321 GAGAAGCATG GCGAACCTTC GTCTACGGCT TCTATTTCTA CAAGATAGTC ACGGACAGAG 
     4381 AGCCCGAACG AGTGGCTATG CAGCAGCCCT CTGCCCCATG GCACACAGCA GGAGTGGATG 
     4441 ACTTTGCCAG CTTCCAACGC AAGTGGTTTG AGGTGGCCTT TGTGGCAGAA GAGCTTGTGC 
     4501 ACTCCGAGAT TCCTGCCTTC CTCCTGCCCT GGCTACCTAG CCGGCCAGCC TCTTACGCAA 
     4561 GCAGGCACAG CTCCTTTAGC CGAAGTTTTG GAGGACGGAG CCAGGCAGCT GCACTGTTAG 
     4621 CTGCCACTGT CCCAGAGCAG AGGACTGTGA CCCTGGATGT CGATGTGAAC AACCGAACGG 
     4681 ACCGGCTGGA GTGGTGCAGC TGTTACTACC ACGGAAACTT CTCTCTCAAC GCAGCCTTTG 
     4741 AAATCAAGCT ACACTGGATG GCAGTGACTG CCACAGTGCT GTTTGAGATG GTCCAAGGTT 
     4801 GGCATCGAAA AGCTACATCC TGCGGCTTCT TGTTAGTCCC GGTTTTGGAG GGTCCTTTTG 
     4861 CACTGCCCAG TTACCTGTAC GGTGACCCCT TGAGGGCCCA GCTCTTCATC CCGCTCAACC 
     4921 TCGGCTGCTT GCTCAAGGAG GGCAGCGAGC ACCTGTTCGA TAGCTTTGAA CCAGAGACAT 
     4981 ATTGGGATCG AATGCACCTT TTCCAGGAAG CCATTGCACA CAGGTTTGGA TTTGTGCAAG 
     5041 ATAAATATTC TGCCTCCGCG TTTAACTTCC CTGCTGAGAA TAAACCACAG TATATCCACG 
     5101 TCACAGGAAC AGTGTTTCTT CAGCTGCCGT ACTCCAAACG CAAGTTTTCT GGGCAGCAGC 
     5161 GGCGGCGACG GAACTCTACC AGCTCCACCA ACCAAAACAT GTTCTGTGAG GAGCGGGTTG 
     5221 GCTACAACTG GGCCTATAAC ACCATGCTGA CCAAGACATG GCGCTCTAGT GCCACGGGGG 
     5281 ATGAGAAGTT TGCCGATCGG CTGCTGAAGG ACTTCACGGA TTTCTGTATC AACCGTGACA 
     5341 ACCGCCTGGT CACATTCTGG ACAAACTGCC TGGAGAAGAT GCACGCCAGC GCTCCCACGC 
     5401 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5461 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5521 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5581 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5641 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5701 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5761 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5821 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5881 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5941 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     6001 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     6061 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     6121 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     6181 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     6241 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     6301 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6361 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6421 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6481 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6541 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6601 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6661 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6721 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6781 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6841 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6901 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6961 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     7021 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     7081 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     7141 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     7201 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     7261 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     7321 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7381 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7441 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7501 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7561 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7621 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7681 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7741 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7801 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7861 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7921 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7981 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     8041 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     8101 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     8161 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     8221 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     8281 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     8341 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8401 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8461 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8521 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8581 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8641 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8701 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8761 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8821 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8881 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8941 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     9001 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     9061 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     9121 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     9181 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     9241 GCGAATTTT
//