LOCUS       RR206228                8886 bp    DNA     circular  SYN 
DEFINITION  Rptor (Myc-DDK-tagged ORF) - Rat similar to p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats (RGD1311784), (10 ug)
ACCESSION   NM_001134499
VERSION     NM_001134499.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5043..5134 
                     /label="Myc-DDK"  
     misc_feature    5164..5749 
                     /label="PolyA signal"  
     misc_feature    5221..5240 
                     /label="Restriction Enzyme"  
     misc_feature    6025..6697 
                     /label="ColE1"  
     misc_feature    7127..7921 
                     /label="Kan/Neo"  
     misc_feature    7956..8258 
                     /label="SV40 ori"  
     misc_feature    8344..8821 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5025..5031 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5025 
                     /label="Rptor"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGTCGGAG ATGCTGCAGT CGCCTCTTAT GGGACTCGGG GAGGAAGATG 
     1081 AGGCAGACCT TACAGATTGG AACTTGCCTT TGGCGTTTAT GAAAAAGAGA CATTGTGAGA 
     1141 AAATTGAAGG CTCCAAATCT TTAGCACAGA GCTGGAGAAT GAAGGATCGG ATGAAGACCG 
     1201 TCAGTGTGGC CTTGGTCCTG TGCCTGAATG TGGGTGTGGA TCCTCCAGAT GTGGTGAAGA 
     1261 CCACACCTTG TGCTCGATTG GAATGCTGGA TTGATCCTCT GTCTATGGGT CCTCAGAAAG 
     1321 CTCTGGAAAC CATCGGTGCA AATCTACAGA AGCAGTATGA GAACTGGCAG CCAAGGGCTC 
     1381 GGTACAAGCA GAGCCTTGAC CCCACTGTGG ATGAAGTCAA GAAACTTTGC ACATCTCTGC 
     1441 GTCGGAACGC CAAGGAGGAA CGGGTCCTCT TCCACTATAA TGGCCATGGG GTGCCCAGGC 
     1501 CTACGGTGAA TGGAGAGGTC TGGGTCTTCA ACAAGAACTA CACTCAGTAC ATCCCACTGT 
     1561 CCATATATGA CCTGCAGACG TGGATGGGCA GCCCATCCAT CTTTGTCTAC GACTGCTCCA 
     1621 ATGCTGGCCT CATCGTCAAG TCCTTCAAAC AGTTCGCACT GCAGAGGGAG CAGGAACTAG 
     1681 AGGTAGCTGC AATTAACCCA AACCATCCAC TTGCCCAGAT GCCCTTGCCT CCCTCAATGA 
     1741 AAAACTGCAT CCAGCTGGCA GCCTGTGAGG CCCACGAGCT CCTGCCGATG ATCCCTGACC 
     1801 TCCCAGCCGA CCTGTTCACA TCCTGCCTCA CCACTCCCAT CAAGATTGCT TTGCGCTGGT 
     1861 TTTGCATGCA GAAGTGTGTT AGTCTGGTGC CTGGAGTCAC ACTGGATTTG ATAGAAAAGA 
     1921 TCCCTGGCCG GCTGAATGAC CGGAGAACCC CTCTGGGTGA ACTGAACTGG ATCTTCACAG 
     1981 CCATCACAGA CACCATCGCC TGGAATGTGC TCCCTCGGGA TCTCTTTCAA AAGCTTTTCC 
     2041 GACAGGACCT GCTGGTGGCA AGTTTGTTTA GAAATTTTTT ATTGGCAGAA AGAATCATGA 
     2101 GGTCGTATAA CTGCACCCCA GTAAGCAGCC CACGACTTCC CCCAACATAC ATGCATGCCA 
     2161 TGTGGCAGGC CTGGGACCTT GCTGTGGACA TTTGCCTCTC TCAACTGCCA ACCATCATCG 
     2221 AAGAGGGAAC TGCCTTCAGG CACAGCCCAT TCTTTGCTGA GCAGCTGACG GCATTCCAGG 
     2281 TGTGGCTCAC CATGGGTGTG GAAAACAGGA GCCCCCCCGA GCAGCTGCCC ATCGTCCTAC 
     2341 AGGTGCTGCT AAGCCAGGTG CACCGGCTGA GAGCCTTGGA CTTGCTGGGA CGATTTTTGG 
     2401 ACTTGGGCCC TTGGGCGGTG AGCTTGGCCC TGTCGGTGGG CATCTTCCCC TATGTACTGA 
     2461 AGCTGCTTCA GAGCTCGGCC CGAGAGCTGC GGCCACTCCT TGTCTTCATC TGGGCCAAAA 
     2521 TCCTCGCTGT GGACAGTTCA TGCCAGGCTG ACCTCGTGAA GGACAACGGT CACAAGTACT 
     2581 TCCTGTCCGT CTTGGCAGAC CCATACATGC CAGCTGAACA CAGGACCATG ACAGCCTTCA 
     2641 TTCTTGCTGT AATCGTCAAC AGCTACACAA CAGGGCAAGA AGCCTGCCTG CAGGGGAACC 
     2701 TGATTGCCAT CTGTTTGGAG CAGCTCAGTG ACCCACACCC CTTGCTACGC CAGTGGGTGG 
     2761 CCATTTGCCT GGGAAGGATC TGGCAGAACT TTGATTCTGC AAGGTGGTGT GGGGTGAGAG 
     2821 ACAGCGCCCA CGAAAAACTC TATAGCCTCC TCTCCGACCC CATCCCTGAG GTACGATGTG 
     2881 CAGCTGTGTT TGCCCTTGGT ACCTTTGTGG GAAACTCTGC TGAGAGGACA GACCACTCTA 
     2941 CTACCATTGA CCACAATGTG GCCATGATGC TGGCTCAGCT GATTAATGAT GGAAGCCCCA 
     3001 TGGTCCGGAA GGAGCTGGTG GTGGCTCTGA GTCACCTTGT AGTCCAATAT GAGAGCAATT 
     3061 TCTGCACTGT GGCCCTGCAG TTCATGGAAG AGGAAAAGAA CTACCCCTTG CCTTCTCCAG 
     3121 CAGCCACAGA GGGAGGGAGC TTGACTCCAG TTCGAGACAG CCCGTGCACC CCCAGACTCC 
     3181 GGTCAGTGAG CTCCTATGGG AACATCCGAG CCGTCACCAC AGCTAGGAAC CTGAACAAAT 
     3241 CTTTGCAGAA CCTGAGCTTG ACGGAAGAAT CGGGCAGCTC AGTGGCCTTC TCTCCCGGGA 
     3301 ACCTCAGTAC AAGTAGTAGT GCCAGCAGCA CCTTGGGAAG CCCTGAGAAC GAGGAGTATA 
     3361 TCCTGTCCTT CGAGACCATC GACAAGATGC GGCGTGTGAG CTCCTATTCA GCGCTCAACT 
     3421 CCCTCATAGG AGTCTCCTTT AATAGTGTCT ACACTCAGAT TTGGAGAGTC TTACTGCATT 
     3481 TGGCCGCTGA TCCCTACCCA GATGTTTCTG ACTTAGCCAT GAAAGTCCTC AACAGCATTG 
     3541 CTTACAAGGC CACAGTCAAC GCCCGGCCCC AGCGAATCCT CGACACATCC TCTCTCACAC 
     3601 AGTCGGCCCC AGCCAGCCCA ACCAACAAGG GCATGCACAT CCACCAGGTG GGAGGCTCCC 
     3661 CGCCAGCATC TAGCACCAGC AGCTGCAGCC TGACCAATGA TGTGGCCAAG CAGACGGTCA 
     3721 GCCGTGACCT GCCCTCCAGC CGCCCGGGTA CTGCCGGCCC CACAGGGGCA CAGTACACCC 
     3781 CTCACTCCCA CCAGTTCCCT CGTACACGAA AGATGTTTGA CAAAGGCCCT GATCAGACCA 
     3841 CAGACGATGC AGATGATGCT GCCGGACACA AGAGCTTCAT TTGTGCCACT ATGCAGACGG 
     3901 GGTTCTGTGA CTGGAGTGCC CGCTACTTTG CCCAGCCGGT CATGAAGATC CCTGAAGAGC 
     3961 ATGACCTAGA GAGTCAGATC CGCAAGGAGC GTGAGTGGCG GTTCCTGAGG AACACTCGAG 
     4021 TCAGGAAGCA GGCACAGCAG GTCATCCAGA AGGGCATCAC CAGACTGGAT GATCAGATAT 
     4081 TTCTGAACAG GAACCCCGGC GTTCCCTCTG TGGTCAAATT CCACCCCTTT ACACCATGCA 
     4141 TAGCTGTTGC TGACAAGGAC AGCATCTGTT TTTGGGACTG GGAGAAAGGA GAGAAACTGG 
     4201 ACTACTTCCA CAATGGTAAC CCTCGGTACA CCAGAGTCAC TGCTATGGAG TACCTGAATG 
     4261 GGCAGGACTG CTCCTTGCTG CTGACAGCCA CAGACGATGG AGCCATCAGG GTCTGGAAAA 
     4321 ATTTTGCTGA TTTGGAAAAG AATCCAGAGA TGGTGACTGC GTGGCAAGGG CTCTCAGACA 
     4381 TGCTTCCAAC AACACGAGGA GCTGGGATGG TGGTGGACTG GGAACAAGAA ACTGGCCTCC 
     4441 TCATGAGCTC AGGGGACGTG CGCATTGTCC GGATCTGGGA TACCGACCGG GAGACGAAGG 
     4501 TCCAGGACAT CCCCACGGGG GCCGACAGCT GTGTGACAAG TTTATCTTGT GATTCGCACC 
     4561 GCTCACTCAT TGTAGCTGGC CTGGGTGATG GCTCGATCCG TGTCTACGAC AGGAGGATGG 
     4621 CACTCAGTGA ATGCCGGGTC ATGACTTACC GAGAGCACAC AGCATGGGTG GTAAAGGCCT 
     4681 ACCTGCAGAA GCACCCTGAG GGCCACATCG TGAGTGTGAG TGTCAATGGA GATGTGCGAT 
     4741 TCTTCGATCC TCGGATGCCT GAGTCTGTGA ATGTAATGCA GATTGTGAAG GGGCTGACAG 
     4801 CCCTCGACAT CCACCCCCAG GCAAACCTGA TCGCCTGTGG CTCCATGAAC CAGTTCACAG 
     4861 CCATCTACAA CGGCAATGGG GAGCTGATCA ACAACATCAA ATACTACGAT GGCTTCATGG 
     4921 GCCAGCGAGT CGGGGCCATC AGCTGCCTGG CTTTCCACCC ACACTGGCCT CATCTGGCCG 
     4981 TCGGAAGCAA TGACTACTAC ATTTCTGTAT ATTCGGTGGA GAAGCGTGTC AGAACGCGTA 
     5041 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     5101 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     5161 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     5221 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5281 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5341 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5401 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5461 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5521 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5581 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5641 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5701 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5761 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5821 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5881 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5941 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     6001 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     6061 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     6121 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     6181 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6241 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6301 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6361 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6421 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6481 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6541 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6601 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6661 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6721 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6781 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6841 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6901 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6961 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     7021 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     7081 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     7141 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     7201 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7261 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7321 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7381 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7441 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7501 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7561 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7621 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7681 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7741 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7801 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7861 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7921 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7981 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     8041 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     8101 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     8161 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     8221 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8281 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8341 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8401 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8461 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8521 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8581 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8641 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8701 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8761 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8821 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8881 AATTTT
//