LOCUS       RR206203                7602 bp    DNA     circular  SYN 
DEFINITION  Fig4 (Myc-DDK-tagged ORF) - Rat FIG4 homolog (S. cerevisiae) (Fig4), (10 ug)
ACCESSION   NM_001047096
VERSION     NM_001047096.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3759..3850 
                     /label="Myc-DDK"  
     misc_feature    3880..4465 
                     /label="PolyA signal"  
     misc_feature    3937..3956 
                     /label="Restriction Enzyme"  
     misc_feature    4741..5413 
                     /label="ColE1"  
     misc_feature    5843..6637 
                     /label="Kan/Neo"  
     misc_feature    6672..6974 
                     /label="SV40 ori"  
     misc_feature    7060..7537 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3741..3747 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3741 
                     /label="Fig4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCACGGCC GCTGCCCCCA TCATCAGTTC GGTCCAGAAG CTGGTGCTCT 
     1081 ATGAGACTAG AGCTAGATAC TTTCTAGTCG GGAGCAATCA TGCGGAAACA AAGTTCCGAG 
     1141 TCCTGAAGAT TGATAGAACA GAACCAAAGG ATTTGGTGGT GATTGACGAT AGGCACGTGT 
     1201 ACACGCAACA GGAAGTGCGG GAACTTCTTG GCCGCCTGGA TCTGGGAAAC AGAACAAAGC 
     1261 TGAGCCAGAA AGGATCCTCC GGGTTGTTTC GAGCCGTCTC CGCGTTTGGT GTCGTAGGTT 
     1321 TTGTCAGGTT CTTAGAAGGC TACTATATCG TATTAATAAC TAAGCGGAGG AAGATGGCGG 
     1381 ATATCGGAGG CCATGCAATA TATAAGATTG AAGATACAAG TATGATTTAT ATCCCCAATG 
     1441 ACTCTGTTCG GATTACTCAC CCCGACGAAG CTAGGTACCT AAGGATATTC CAAAATGTGG 
     1501 ATCTGTCCAG CAATTTTTAC TTTAGTTACA GCTATGATCT GTCCCACTCC CTTCAGTACA 
     1561 ATCTTACCGT CCTGCGAATG CCCCTGGAGA TGTTAAAGTC GGAAACATCC AAGACCTGCC 
     1621 AGGAGAGCTT CGACATCTTT GAAGATGAAG GGTTAACTAC GCAGGGCGGC AGTGGCGTAT 
     1681 TTGGGATCTC TAGTGAGCCT TACATGAAGT ATGTCTGGAA CGGCGAACTT CTGGATATAA 
     1741 TTAAAAACAG CGTGCATCGC GACTGGCTAC TGTATATCAT TCATGGGTTC TGCGGGCAGT 
     1801 CAAAGCTTCT GATCTATGGA CGACCAGTGT ATGTGACCCT AATAGCCAGA AGATCCAGTA 
     1861 GGTTTGCTGG GACGCGCTTT CTCAAGAGAG GTGCAAACTG TGAGGGAGAT GTTGCCAACG 
     1921 AGGTGGAGAC GGAGCAGATC CTCTGTGATG CCTCGGTGAT GTCTTTTACC GCAGGCAGTT 
     1981 ACTCTTCTTA CGTACAAGTC AGAGGCTCTG TTCCCTTATT CTGGTCCCAA GACATCTCAA 
     2041 CCATGATGCC GAAACCACCC ATTACACTGG ACCAGGCAGA TCCCTTCGCA CACATAGCCG 
     2101 CTCTTCACTT TGACCAGATG CTGCAGAGGT TTGGCTCCCC CATCATCATC CTGAACTTAG 
     2161 TGAAGGAGAG AGAGAAGAGA AAGCACGAAA GGATTCTGAG CGAGGAGCTG GTGGCCGCGG 
     2221 TTACTTACCT TAACCAGTTC CTGCCCCCGG AGCACAGCAT TGTATACATT CCCTGGGATA 
     2281 TGGCCAAGTA CACCAAGAGT AAGCTGTGTA ACGTTCTCGA TCGTCTGAAC GTGATTGCAG 
     2341 AAAGCGTGGT AAAGAAGACA GGCTTCTTCG TCAACCGCCC TGATTCTTAC TGCAGCGTTT 
     2401 TACGGCCAGA TGAGAAGTGG AACGAACTAG GAGGTCGTGT GATTCCCACT GGTCGGCTCC 
     2461 AGACCGGCAT CCTTCGAACC AACTGCGTGG ACTGTTTGGA TCGTACCAAC ACGGCGCAGT 
     2521 TCATGGTGGG CAAGTGCGCC CTGGCTTACC AGCTGTACTC CTTAGGGCTG ATTGACAAGC 
     2581 CCAACCTGCA GTTCGATACG GACGCAGTGA GGTTATTTGA GGAACTCTAT GAAGACCATG 
     2641 GAGACACCCT TTCCCTGCAG TACGGAGGCT CTCAGCTGGT TCATCGGGTG AAGACCTACA 
     2701 GAAAGATAGC GCCGTGGACC CAGCACTCCA AGGACATCAT GCAGACGCTG TCTCGGTACT 
     2761 ACAGCAATGC CTTTTCAGAT GCGGACAGAC AAGATTCCAT CAACCTGTTC TTGGGTGTTT 
     2821 TCCACCCCAC AGAAGGGAAA CCTCATCTCT GGGAACTTCC CACGGACTTT TACCTGCATC 
     2881 ACAAAAACAC CATGAGCCTT TTACCCCCCA AAAGGAGTTA TACTCACTGG TGGACACCAG 
     2941 AGGTGGTGAA GCATTTGCCG CTGCCTTACG ACGAAGTTAT CTGTGCTGCA AACTTAAAGA 
     3001 AGTTGACGGT GAAGAAGTTT CACAGATGGG AAGAGGAGAT TGATATTCAC AATGAGTTCT 
     3061 TCCGGCCGTA TGAATTGAGT AGTTTTGATG ACACCTTTTG CCTGGCCATG ACAAGTTCAG 
     3121 CACGTGATTT TATGCCTAAA ACTATTGGCA TTGACCCAAG TCCATTCACC GTGCGAAAAC 
     3181 CAGACGAAAC CGGGAAATCT GTACTGGGGA ACAAAAGCAA CAGAGAGGAG GCCGTCCTGC 
     3241 AACGGAAAAC GGCAGCCAGC GCCCCGCCAC CCCCCAGCGA GGAGGCCGTG TCCAGCAGCT 
     3301 CCGAGGACGA CTCTGGTACT GACCGGGAAG ACGAAGGTTC CATCTCGCAG CGATCCACTC 
     3361 CTGTGAAGAT GACTGACACC GGAGACAGCG CCAAAGTGAC TGAGAGTGTG GCCCAACCCA 
     3421 TGAAGGAAGT GTACGGAGTT AGCCTCTCAC GTAGCCTGTC CGAGGAGGAT CACTCCATTT 
     3481 ATGCCAGGTT TGTTCAACTG GGGCAGAGTC AGCATAGACA GGACTGGAGC AGCCCGCAGA 
     3541 TGTGTGCCCG GTGCTCAGAC GGAGTTATCA AACTAACCCC CATCTCAGCT TTCTCGCAAG 
     3601 ACAACATTTA TGAAGTTCAG CCCCCGAGAG TAGACAGAAA GTCCACAGAG ATCTTCCAGG 
     3661 CCCACATCCA GGCCAGCCAA GGTATCATGC AGCCCCTTGG CAAAGAGGAC ACTGCCATAT 
     3721 ACCGGGAGTA CATCAGAAAC CGCTACTTAA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3781 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3841 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3901 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3961 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4021 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4081 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4141 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4201 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4261 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4321 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4381 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4441 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4501 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4561 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4621 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4681 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4741 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4801 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4861 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4921 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4981 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5041 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5101 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5161 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5221 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5281 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5341 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5401 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5461 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5521 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5581 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5641 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5701 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5761 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5821 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5881 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5941 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6001 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6061 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6121 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6181 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6241 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6301 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6361 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6421 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6481 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6541 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6601 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6661 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6721 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6781 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6841 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6901 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6961 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7021 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7081 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7141 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7201 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7261 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7321 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7381 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7441 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7501 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7561 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//