LOCUS       RR206199                8781 bp    DNA     circular  SYN 
DEFINITION  Ambra1 (Myc-DDK-tagged ORF) - Rat ischemia related factor NYW-1 (Nyw1), (10 ug)
ACCESSION   NM_001134341
VERSION     NM_001134341.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4938..5029 
                     /label="Myc-DDK"  
     misc_feature    5059..5644 
                     /label="PolyA signal"  
     misc_feature    5116..5135 
                     /label="Restriction Enzyme"  
     misc_feature    5920..6592 
                     /label="ColE1"  
     misc_feature    7022..7816 
                     /label="Kan/Neo"  
     misc_feature    7851..8153 
                     /label="SV40 ori"  
     misc_feature    8239..8716 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4920..4926 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4920 
                     /label="Ambra1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAGTTGTC CCAGAGAAGA ATGCTGTACG AATCCTCTGG GGCCGAGAGC 
     1081 GGGGCACTCG TGCCATGGGT GCCCAGCGGC TTCTGCAGGA GCTGGTGGAG GATAAGACTC 
     1141 GATGGATGAA ATGGGAGGGC AAGAGAGTAG AACTTCCTGA TAGTCCACGC TCGACCTTCT 
     1201 TACTGGCCTT CAGCCCAGAC AGGACTCTCT TGGCGTCCAC ACATGTGAAC CATAACATCT 
     1261 ATATTACAGA GGTTAAGACT GGCAAGTGTG TTCACTCTCT GATTGGACAC CGCCGTACTC 
     1321 CATGGTGTGT CACTTTTCAT CCCACTATCT CAGGCCTTAT TGCTTCTGGC TGCCTAGATG 
     1381 GGGAAGTTAG GATTTGGGAC TTACATGGTG GCAGTGAAAG CTGGTTCACA GATAGCAACA 
     1441 ATGCCATAGC TTCTTTGGCT TTCCACCCTA CGGCTCAGCT CCTGCTGATC GCCACTGCAA 
     1501 ATGAGATCCA TTTCTGGGAT TGGAGTCGTC GGGAACCCTT TGCTGTGGTG AAAACAGCTA 
     1561 GTGAGATGGA GCGTGTTCGT CTGGTGAGAT TTGATCCACT TGGACACTAC TTACTTACAG 
     1621 CTATTGTTAA CCCCTCTAAT CAGCAGGGTG ATGATGAACC AGAAATCCCT ATAGATGGAA 
     1681 CAGAATTATC TCATTACCGT CAGCGTGCCC TCCTGCAATC ACAGCCAGTT CGCCGGACAC 
     1741 CCCTCCTCCA CAATTTCCTG CATATGCTGT CCTCCCGCTC CTCTGGCATC CAGGTGGGAG 
     1801 AGCAAAGCAC AGTACAAGAT TCTGCTACCC CCTCACCCCC ACCGCCTCCC CCTCAGCCCT 
     1861 CCACGGAGCG TCCCAGGACT TCCGCTTACA TCAGACTCCG ACAGCGGGTC AGTTACCCCA 
     1921 CCACAGTTGA GTGCTGCCAG CACCCTGGGA TCCTGTGCCT TTGCAGCCGT TGTGCTGGCA 
     1981 CTCGAGTTCC TTCCCTCTTG CCACACCAGG ACAGTGTCCC CCCTGCTTCT GCCAGAGCTA 
     2041 CTACCCCTTC CTTTTCTTTT GTACAGACCG AGCCCTTCCA TCCCCCGGAG CAGGCTTCAT 
     2101 CAACGCAGCA GGACCAAGGC CTCCTGAACC GGCCGTCTGC CTTCAGTACA GTCCAGAGCA 
     2161 GCACTGCGGG CAACACGCTC CGCAACCTCA GTCTGGGTCC TACCCGTCGC TCTTTGGGGG 
     2221 GCCCACTGTC CAGCCACCCA TCTAGGTATC ACAGAGAACT AGCCCCTGGG CTGACAGGTT 
     2281 CTGAGTGGAC CCGGACAGTA CTCACTCTGA ACTCACGTTC TGAGGTAGAA TCTATGCCCC 
     2341 CACCGAGGAC CAGTGCCTCC TCAGTGAGTT TGCTTTCTGT ACTGAGACAG CAGGAAGGTG 
     2401 GCTCTCAGGC ATCTGTGTAT ACTTCAGCCA CAGAAGGGAG GGGTTTTCCA TCATCAGGGT 
     2461 TGGCAACTGA GTCAGATGGA GGGAATGGTT CCAGCCAAAA CAACTCAGGC AACATTCGCC 
     2521 ATGAGCTTCA GTGTGACCTG AGACGCTTTT TTTTGGAGTA TGACAGGCTG CAGGAGCTGG 
     2581 ATCAGAGCCT GAGTGGGGAA ACTCCCCAGA CCCAACAGGC CCAGGAAATG CTCAACAATA 
     2641 ATATTGAATC TGAGAGACCA GGTCCTTCCC ATCAGCCTAC CCCACACAGC AGTGAGAACA 
     2701 ACTCCAACCT TTCTCGGGGC CACCTTAATC GATGCCGTGC TTGCCATAAT CTTCTAACCT 
     2761 TCAACAATGA TACCCTTCGT TGGGAAAGAA CCACACCTAA CTACTCATCC GGTGAGGCTA 
     2821 GCTCTTCCTG GCATGTCTCC ACTACTTTTG AGGGCATGCC ACCGAGTGGC AACCAACTAC 
     2881 CACCTCTTGA GCGGACTGAG GGCCAAATGC CTAACTCCAG CAGGCTGGAG TTGAGCAGTT 
     2941 CTGCTAGTTC ACAAGAGGAG AGGACTGTGG GGGTGGCCTT TAACCAGGAG ACCGGCCATT 
     3001 GGGAACGAAT TTATACCCAG TCCAGCAGAT CTGGAACTGT ATCACAGGAG GCCTTACATC 
     3061 AAGATATGCC TGAGGAAAGC TCTGAGGAAG ACTCCCTGAG GAGGAGGCTG CTGGAGTCTT 
     3121 CCCTCATTTC ATTATCCCGT TATGATGGAG CAGGATCCAG AGAGCACCCA ATTTACCCAG 
     3181 ACCCAGCGAG ATTATCTCCT GCTGCATACT ACGCCCAGAG GATGATCCAG TATCTCTCAA 
     3241 GGAGAGACAG TATTCGCCAG CGCTCCATGC GCTACCAACA GAATCGGCTT CGTTCTTCCA 
     3301 CCTCCTCTTC TTCCTCAGAC AACCAGGGTC CATCAGTAGA GGGAACCGAC TTGGAATTTG 
     3361 AGGACTTTGA GGACAATGGT GACAGATCTA GGCACCGAGC TCCCCGAAAT GCCCGAATGT 
     3421 CTGCACCATC ACTTGGACGC TTTGTCCCAA GGCGTTTTTT GCTGCCTGAG TACTTGCCTT 
     3481 ATGCTGGGAT TTTTCATGAG CGTGGACAGC CTGGCTTGGC TACTCATTCT TCTGTTAACA 
     3541 GGGTCCTGGC AGGGGCAGTA ATTGGAGATG GACAGTCTGC TGTGGCCAGT AACATTGCCA 
     3601 ATACTACCTA CAGGCTCCAG TGGTGGGACT TCACCAAGTT TGACCTTCCA GAAATCAGTA 
     3661 ATGCCTCCGT GAATGTCCTG GTACAGAACT GCAAGATCTA CAATGATGCC AGCTGTGACA 
     3721 TTTCTGCAGA TGGCCAGCTC CTGGCAGCTT TCATCCCGAG TAGCCAGAGG GGCTTTCCTG 
     3781 ATGAAGGCAT CCTGGCAGTG TATTCCCTGG CCCCCCATAA CTTGGGGGAA ATGCTCTACA 
     3841 CCAAGCGATT TGGTCCCAAT GCCATTTCTG TAAGCCTGTC CCCAATGGGC CGATACGTTA 
     3901 TGGTGGGCTT GGCCTCACGA AGGATCCTGC TGCACCCCTC TACAGAGCAC ATGGTAGCCC 
     3961 AGGTCTTCAG GCTGCAGCAG GCCCATGGTG GTGAGACTTC CATGAGGAGA GTTTTCAACG 
     4021 TCCTTTACCC CATGCCTGCG GACCAGCGGA GACACGTCAG CATCAACTCT GCCCGCTGGC 
     4081 TACCTGAGCC AGGGCTCGGT TTGGCCTATG GCACCAACAA AGGAGACCTG GTGATCTGCC 
     4141 GACCAGAGGC CTTAAACTCT GGCATTGAGT ACTACTGGGA CCAGCTAAAT GAAACGGTCT 
     4201 TCACTGTCCA CTCAAGTAGC AGGAGCAGCG AGCGGCCTGG AACCAGCAGA GCCACATGGC 
     4261 GGACAGACAG AGACATGGGC TTGATGAATG CAATTGGGCT GCAACCTCGG AACCCTACCA 
     4321 CCTCTGTGAC ATCTCAGGGC ACCCAGACTC TGGCCCTCCA GTTGCAGAAT GCTGAAACAC 
     4381 AGACTGAGAG GGAGGAAGAG GAACCAGGGA CAGCATCCTC AGGTCCTGGT GAAGGCGAGG 
     4441 GCTCGGAATA TGGTGGCAGT GGGGAAGATG CCCTCAGCCG GATCCAGAGG CTAATGGCCG 
     4501 AGGGTGGCAT GACAGCTGTG GTACAGCGAG AACAGAGTAC CACCATGGCC TCCATGGGTG 
     4561 GCTTCGGCAA CAACATCATC GTTAGCCACC GCATTCACCG CAGCTCCCAG ACAGGCACAG 
     4621 AGTCAGGGGC TGCTCGTACC TCCTCACCCC AGCCCTCCAC CTCTCGAGGA CTACTCTCAG 
     4681 AACCTGGGCA ACTGGCAGAG CGAGGACTAA GCCCCCGGAC AGCCTCCTGG GACCAGCCTA 
     4741 GTACCTCTGG AAGGGAACTA CCCCAGCCAG CCCTGTCCTC TTCCTCCCCT GTCCCCATTC 
     4801 CAGTCCCCCT TGCCAGCAAT GAGGGACCAA CCATGCACTG CAATGTGACC AATAACAGTC 
     4861 ACCTTCCTGA GGGTGATAGT AGCAATGTGG GGGAAGCTGC AGGCCCTAGT GGGGAGCCAC 
     4921 GGAACCGAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4981 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5041 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5101 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5161 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5221 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5281 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5341 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5401 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5461 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5521 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5581 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5641 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5701 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5761 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5821 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5881 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5941 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6001 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6061 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6121 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6181 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6241 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6301 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6361 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6421 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6481 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6541 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6601 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6661 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6721 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6781 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6841 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6901 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6961 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7021 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7081 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7141 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7201 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7261 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7321 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7381 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7441 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7501 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7561 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7621 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7681 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7741 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7801 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7861 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7921 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7981 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8041 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8101 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8161 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8221 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8281 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8341 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8401 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8461 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8521 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8581 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8641 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8701 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8761 CAAAAATTTA ACGCGAATTT T
//