LOCUS       RR206192                7980 bp    DNA     circular  SYN 
DEFINITION  Pear1 (Myc-DDK-tagged ORF) - Rat platelet endothelial aggregation receptor 1 (Pear1), (10 ug)
ACCESSION   NM_001134959
VERSION     NM_001134959.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4137..4228 
                     /label="Myc-DDK"  
     misc_feature    4258..4843 
                     /label="PolyA signal"  
     misc_feature    4315..4334 
                     /label="Restriction Enzyme"  
     misc_feature    5119..5791 
                     /label="ColE1"  
     misc_feature    6221..7015 
                     /label="Kan/Neo"  
     misc_feature    7050..7352 
                     /label="SV40 ori"  
     misc_feature    7438..7915 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4119..4125 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4119 
                     /label="Pear1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCACTCTGT CCCCTCCTCC TCCTGGCCCT AGGCCTGCGT CTGGCTGGAA 
     1081 CACTCAACTC CAATGATCCC AATGTCTGTA CCTTCTGGGA AAGCTTCACC ACGACCACTA 
     1141 AGGAGTCCCA CCTTCGCCCC TTCAGCCTGC CCCCAGCCGA GTCCTGCGAC AGGCCCTGGG 
     1201 AAGACCCCCA CACCTGCGCT CAGCCTATGG TTGTCTACCG GACTGTGTAC CGTCAGGTGG 
     1261 TGAAGATGGA CTCCCGCCCA CGCCTGCAGT GCTGTGGGGG TTACTACGAG AGCAGTGGAG 
     1321 CCTGTGTCCC ACTCTGTGCC CAGGAGTGTG TCCACGGTCG CTGTGTGGCT CCTAATCGGT 
     1381 GCCAGTGTGC ACCAGGCTGG CGGGGTGACG ACTGTTCCAG TGAGTGTGCT CCTGGAATGT 
     1441 GGGGACCACA GTGTGACAGG CTCTGCCTCT GTGGCAACAG CAGTTCCTGT GATCCCAGGA 
     1501 GTGGGGTGTG TTTTTGCCCC TCTGGCCTGC AGCCCCCCGA CTGCCTTCAG CCTTGCCCCG 
     1561 ATGGCCACTA TGGTCCTGCC TGCCAGTTTG ATTGCCATTG CTATGGGGCA TCCTGTGACC 
     1621 CCCGGGATGG AGCCTGCTTC TGCCCCCCAG GGAGAACAGG ACCCAGCTGT AATGTGCCCT 
     1681 GTTCACAGGG CACTGATGGC TTCTTCTGCC CCAGAACTTA TCCTTGCCAA AATGGAGGTG 
     1741 TTCCTCAGGG CTCTCAAGGC TCCTGCAGCT GCCCACCGGG CTGGATGGGT GTCATCTGTT 
     1801 CCCTGCCATG CCCAGAGGGT TTCCACGGAC CCAACTGTAC TCAGGAATGT CGTTGCCACA 
     1861 ATGGTGGCCT TTGTGACAGG TTTACTGGGC AGTGCCACTG TGCTCCTGGC TATATCGGGG 
     1921 ATCGGTGCCG TGAAGAGTGC CCTGTGGGCC GCTTCGGTCA AGACTGTGCT GAGACCTGTG 
     1981 ACTGTGCTCC TGGCGCTCGT TGCTTTCCTG CCAATGGCGC GTGTCTGTGC GAACATGGCT 
     2041 TCACAGGCGA CCGCTGCACT GAGCGACTCT GTCCAGATGG CCGCTATGGT CTGAGCTGCC 
     2101 AAGATCCCTG CACCTGCGAC CCAGAACACA GTCTCAGCTG CCACCCAATG CACGGCGAGT 
     2161 GCTCCTGCCA GCCAGGTTGG GCGGGCCTCC ACTGCAACGA GAGCTGCCCT CAGGACACGC 
     2221 ACGGAGCCGG TTGCCAGGAG CACTGCCTCT GTCTGCACGG CGGTGTTTGC CTCGCCGACA 
     2281 GCGGCCTCTG CCGGTGTGCA CCTGGCTACA CGGGACCTCA CTGCGCTAAT CTTTGTCCAC 
     2341 CTAACACTTA TGGGATCAAC TGTTCCTCCC ACTGCTCCTG TGAAAATGCC ATTGCCTGCT 
     2401 CTCCTGTCGA CGGCACGTGC ATCTGCAAGG AAGGTTGGCA GCGTGGTAAC TGCTCTGTGC 
     2461 CCTGTCCCCC TGGCACCTGG GGCTTCAGTT GCAATGCCAG TTGCCAGTGT GCCCACGAGG 
     2521 GAGTCTGCAG CCCCCAAACT GGAGCCTGTA CTTGCACCCC TGGGTGGCGT GGGGTTCACT 
     2581 GCCAACTTCC GTGCCCGAAG GGACAGTTTG GTGAAGGTTG TGCCAGTGTC TGTGACTGTG 
     2641 ACCACTCCGA TGGCTGTGAC CCTGTTCATG GACACTGCCG ATGTCAGGCT GGCTGGATGG 
     2701 GCACACGTTG CCACCTGCCT TGCCCAGAGG GCTTTTGGGG AGCCAACTGC AGCAATGCCT 
     2761 GTACCTGCAA GAATGGTGGC ACTTGTGTAC CTGAGAACGG CAACTGTGTG TGCGCACCAG 
     2821 GGTTCAGAGG CCCCTCCTGC CAGAGGCCCT GCCCGCCTGG TCGCTATGGC AAACGCTGTG 
     2881 TGCCCTGCAA GTGCAACAAC CATTCTTCCT GCCACCCGTC GGATGGGACC TGCTCCTGCC 
     2941 TGGCAGGCTG GACAGGCCCT GACTGCTCTG AATCATGTCC CCCAGGCCAC TGGGGACTCA 
     3001 AATGCTCCCA ACCCTGCCAG TGTCATCATG GTGCCACCTG CCACCCCCAG GATGGGAGCT 
     3061 GTGTCTGCAT CCCAGGCCGG ACTGGACCCA ACTGCTCGGA AGGCTGCCCA TCAAGAATGT 
     3121 TTGGTGTCAA CTGCTCCCAG CTATGTCAGT GTGATCCTGG AGAGATGTGC CACCCAGAGA 
     3181 CTGGGGCTTG CGTCTGTCCC CCAGGACACA GTGGTGCGCA CTGCAAAGTG GGCAGCCAGG 
     3241 AGTCCTTCAC CATAATGCCC ACCTCTCCTG TGATCCATAA CTCACTGGGT GCCGTGATTG 
     3301 GCATTGCAGT GCTGGGGACC CTTGTGGTGG CCCTGGTAGC ACTGTTTATT GGCTACCGAC 
     3361 ACTGGCAAAA GGGCAAGGAA CATGAGCACT TGGCAGTGGC TTACAGCACT GGGCGACTGG 
     3421 ATGGCTCCGA TTACGTCATG CCAGATGTCT CTCCGAGCTA CAGTCACTAC TATTCCAACC 
     3481 CTAGCTACCA CACACTGTCT CAGTGTTCTC CTAACCCTCC ACCCCCTAAC AAGATTCCAG 
     3541 GCAGTCAGCT GTTTGTCAGC TCCCAGGCAT CTGAGCGGCC AAACAGAAAC CATGGGCGAG 
     3601 ATAACCACGC CACACTGCCC GCTGACTGGA AGCACCGACG GGAGTCCCAT GACAGAGGCA 
     3661 CCAGCCACCT GGACCGAAGG TATAGCTGTA GCTATGGCCA CAGGAATGGC CCGGGGCCAT 
     3721 TCTGTCATAA AGGTCCCATC TCTGAAGAAG GACTAGGGGC AAGCGTTATG TCCCTGAGCA 
     3781 GTGAGAACCC CTATGCGACC ATCCGAGACC TGCCCGGCCT GCCTGGGGAA CCCCGAGAAA 
     3841 GCAGCTATGT GGAGATGAAA GGCCCTCCAT CAGTGTCTCC CCCCAGGCAG CCTCTTCATC 
     3901 TCCGGGACAG GCAGCAGCAG CAACTGCAGT CTCAGAGAGA CAGCGGCACC TATGAGCAGC 
     3961 CCACTCCCTT GAGCCGTAAT GAAGAGTCTG TGGGCTCCAT GCCCCCTCTT CCTCCGGGCC 
     4021 TGCCACCCGG CCACTATGAC TCGCCCAAAA ACAGCCACAT CCCTGGACAC TATGACTTGC 
     4081 CTCCAGTACG GCATCCTCCA TCACCTCCAT CCCGGCGCCA GGACCGCACG CGTACGCGGC 
     4141 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4201 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4261 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4321 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4381 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4441 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4501 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4561 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4621 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4681 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4741 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4801 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4861 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4921 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4981 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5041 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5101 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5161 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5221 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5281 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5341 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5401 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5461 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5521 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5581 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5641 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5701 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5761 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5821 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5881 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5941 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6001 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6061 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6121 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6181 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6241 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6301 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6361 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6421 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6481 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6541 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6601 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6661 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6721 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6781 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6841 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6901 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6961 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7021 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7081 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7141 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7201 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7261 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7321 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7381 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7441 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7501 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7561 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7621 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7681 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7741 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7801 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7861 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7921 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//