LOCUS       RR206177                8331 bp    DNA     circular  SYN 
DEFINITION  Slc12a6 (Myc-DDK-tagged ORF) - Rat solute carrier family 12, member 6 (Slc12a6), (10 ug)
ACCESSION   NM_001109630
VERSION     NM_001109630.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4488..4579 
                     /label="Myc-DDK"  
     misc_feature    4609..5194 
                     /label="PolyA signal"  
     misc_feature    4666..4685 
                     /label="Restriction Enzyme"  
     misc_feature    5470..6142 
                     /label="ColE1"  
     misc_feature    6572..7366 
                     /label="Kan/Neo"  
     misc_feature    7401..7703 
                     /label="SV40 ori"  
     misc_feature    7789..8266 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4470..4476 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4470 
                     /label="Slc12a6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCATCCTCCA GAAACCACCA CCAAGATGTC TTCAGTTCGG TTCATGGTGA 
     1081 CACCAACAAA GATTGATGAC ATTCCAGGTT TATCAGACAC CAGTCCGGAC CTCAGCTCTC 
     1141 GATCTAGTTC TCGAGTAAGA TTTAGCTCCC GAGAAAGTGT GCCAGAAACA AGCCGTAGTG 
     1201 AGCCTATGAG TGAACTGTCT GGGGCCACCA CTTCTCTGGC AACTGTTGCC CTAGATCCTT 
     1261 CCAGTGACCG GACTTCTAAC CCCCAGGATG TTACCGAGGA CCCGAATCAG AACTCCATCA 
     1321 CAGGGGAGCA CAGCCAGCTG TTAGATGATG GACATAAGAA AGCTCGAAAT GCTTATCTCA 
     1381 ATAATTCCAA CTATGAAGAA GGAGACGAAT ATTTTGATAA AAATCTGGCA CTCTTTGAGG 
     1441 AAGAAATGGA CACCAGACCA AAGGTGTCTT CTCTCCTCAA CCGCATGGCC AACTATACAA 
     1501 ATCTGACACA AGGAGCAAAG GAACATGAAG AGGCAGAGAA CATCACCGAA GGGAAAAAGA 
     1561 AGCCTACCAA GAGCCCCCAA ATGGGTACTT TCATGGGTGT CTACCTCCCA TGTCTACAAA 
     1621 ATATTTTTGG AGTGATCCTC TTTCTGCGTC TTACCTGGGT AGTGGGCACA GCTGGAGTCC 
     1681 TTCAGGCCTT TGCCATTGTC CTTATCTGCT GCTGCTGTAC AATGTTAACT GCCATCTCCA 
     1741 TGAGTGCCAT CGCCACTAAC GGAGTGGTGC CAGCTGGAGG CTCATACTTC ATGATTTCCC 
     1801 GGGCCCTGGG CCCAGAGTTT GGCGGGGCTG TGGGCCTCTG CTTTTATCTT GGCACCACAT 
     1861 TTGCAGCAGC CATGTATATT CTTGGTGCCA TTGAAATCTT TCTGGTGTAT ATTGTCCCCC 
     1921 GAGCTGCCAT CTTTCGGAGC GATGATGCAC TCAAGGAGTC AGCAGCCATG CTGAACAACA 
     1981 TGCGTGTCTA TGGTACAGCC TTCCTGGTCC TCATGGTCTT GGTGGTGTTC ATCGGCGTAC 
     2041 GCTATGTGAA TAAGTTTGCC TCACTTTTCC TGGCCTGTGT AATTGTGTCG ATCTTGGCTA 
     2101 TCTATGCTGG TGCCATCAAG TCTTCCTTTG CTCCACCACA CTTCCCGGTC TGTATGCTGG 
     2161 GCAACCGCAC CCTGTCATCA AGACACCTTG ACATTTGCTC TAAGACCAAG GAAGTTAACA 
     2221 ACATGACAAT ACCATCAAAG TTATGGGGAT TCTTCTGCAA CTCAAGTCAG TTTTTTAATG 
     2281 CCACCTGTGA TGAGTACTTT GTTCACAATA ATGTCACCTC AATCCAGGGC ATTCCAGGGT 
     2341 TGGCTAGTGG CATCATTACT GAAAACCTTT GGAGTAATTA TTTACCAAAA GGTGAGATAA 
     2401 TTGAAAAGCC ATCAGCCAAG TCATCTGACG TTTTGGGCAA CTTAAACCAT GAATATGTTC 
     2461 TTGCTGATAT TACCACCTCC TTCACTCTTC TGGTGGGGAT CTTCTTTCCT TCTGTCACAG 
     2521 GTATCATGGC TGGGTCAAAC AGATCTGGAG ATCTGAAAGA TGCTCAAAAG TCTATCCCCA 
     2581 TTGGGACCAT CCTTGCCATC CTGACCACAT CCTTCGTGTA TTTAAGCAAT GTTGTCCTTT 
     2641 TTGGTGCATG TATCGAAGGA GTTGTCCTCA GAGACAAATT TGGGGATGCT GTAAAAGGGA 
     2701 ATTTGGTTGT AGGCACCTTA TCCTGGCCAT CCCCATGGGT GATTGTTATT GGCTCCTTCT 
     2761 TTTCAACGTG TGGAGCTGGA CTTCAGAGCC TCACAGGTGC ACCTCGGCTG CTGCAGGCTA 
     2821 TTGCCAAGGA CAACATCATA CCTTTCCTTA GGGTTTTTGG ACACAGTAAA GCTAATGGGG 
     2881 AACCTACCTG GGCCTTACTT CTAACTGCTG CCATAGCAGA GCTGGGAATT CTCATCGCCT 
     2941 CCCTGGATCT CGTGGCCCCA ATTCTTTCCA TGTTTTTTCT CATGTGTTAC CTCTTTGTGA 
     3001 ACTTGGCTTG TGCCTTGCAA ACATTACTGC GAACCCCCCA CTGGAGGCCT CGATTCCGCT 
     3061 ACTATCACTG GGCCCTCTCT TTCATGGGAA TGAGTATCTG TCTAGCTCTG ATGTTCATTT 
     3121 CTTCTTGGTA TTATGCCATT GTAGCTATGG TAATAGCTGG CATGATCTAC AAGTACATTG 
     3181 AATACCAAGG GGCTGAAAAA GAATGGGGCG ATGGTATCCG TGGGCTGTCA CTCAGTGCAG 
     3241 CCCGCTTCGC TTTGCTCCGC CTAGAGGAAG GCCCTCCTCA CACTAAAAAC TGGAGGCCTC 
     3301 AGCTGCTTGT ACTGCTAAAG CTGGATGAAG ACTTACACGT CAAGCACCCT CGCCTCCTCA 
     3361 CCTTTGCCTC ACAGCTCAAG GCAGGAAAGG GACTCACCAT TGTGGGCTCT GTCATCGTGG 
     3421 GGAACTTCCT GGAGAACTAT GGTGACGCTC TAGCAGCAGA GCAGACCATT AAGCACCTCA 
     3481 TGGAGGCAGA AAAGGTAAAA GGGTTCTGCC AGCTGGTAGT GGCTGCCAAG CTGAAAGAGG 
     3541 GCATTTCACA CCTCATCCAG TCCTGTGGCC TTGGAGGCAT GAAGCACAAC ACAGTGGTGA 
     3601 TGGGCTGGCC CAATGGCTGG CGTCAGAGTG AAGATGCTCG TGCTTGGAAG ACTTTCATTG 
     3661 GCACAGTTCG AGTGACAACT GCTGCCCATC TAGCCCTGTT AGTGGCTAAA AATGTCTCCT 
     3721 TCTTTCCCAG CAATGTGGAA CAATTTTCTG AGGGCAACAT TGATGTGTGG TGGATTGTGC 
     3781 ATGATGGGGG CATGCTCATG CTATTACCAT TCCTACTGAA ACAGCACAAG GTTTGGCGAA 
     3841 AATGCAGCAT ACGGATCTTC ACAGTAGCCC AACTAGAAGA CAACAGTATC CAGATGAAGA 
     3901 AGGACCTGGC CACCTTCCTG TACCATCTGC GCATCGAGGC CGAAGTGGAA GTGGTGGAGA 
     3961 TGCATGACAG TGACATATCT GCCTATACTT ATGAGCGCAC CCTGATGATG GAGCAGAGGT 
     4021 CCCAGATGCT TCGGCACATG CGGCTGTCCA AAACAGAACG AGATAGAGAG GCACAACTGG 
     4081 TGAAAGATCG AAACTCAATG CTTCGCTTAA CTAGCATTGG CTCTGACGAG GATGAAGAAA 
     4141 CAGAAACTTA CCAAGAGAAG GTGCACATGA CTTGGACCAA GGATAAATAC ATGGCATCCC 
     4201 GGGGGCAAAA GGTCAAATCA ATGGAAGGAT TCCAAGACCT ACTTAATATG CGTCCGGACC 
     4261 AGTCCAACGT GAGACGGATG CATACAGCAG TGAAGCTCAA TGAAGTTATA GTTAACAAGT 
     4321 CCCACGAAGC AAAGCTGGTT TTGTTGAATA TGCCAGGACC ACCCCGAAAC CCTGAAGGTG 
     4381 ATGAGAACTA CATGGAATTT CTAGAAGTGC TCACTGAGGG ATTAGAGCGA GTCCTCCTCG 
     4441 TCCGGGGTGG TGGCAGTGAA GTCATCACCA TATACTCAAC GCGTACGCGG CCGCTCGAGC 
     4501 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4561 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4621 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4681 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4741 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4801 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4861 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4921 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4981 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5041 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5101 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5161 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5221 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5281 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5341 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5401 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5461 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5521 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5581 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5641 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5701 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5761 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5821 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5881 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5941 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6001 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6061 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6121 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6181 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6241 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6301 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6361 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6421 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6481 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6541 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6601 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6661 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6721 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6781 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6841 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6901 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6961 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7021 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7081 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7141 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7201 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7261 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7321 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7381 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7441 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7501 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7561 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7621 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7681 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7741 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7801 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7861 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7921 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7981 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8041 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8101 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8161 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8221 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8281 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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