LOCUS       RR206110                8022 bp    DNA     circular  SYN 
DEFINITION  Mical1 (Myc-DDK-tagged ORF) - Rat microtubule associated monoxygenase, calponin and LIM domain containing 1 (Mical1), (10 ug)
ACCESSION   NM_001106397
VERSION     NM_001106397.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4179..4270 
                     /label="Myc-DDK"  
     misc_feature    4300..4885 
                     /label="PolyA signal"  
     misc_feature    4357..4376 
                     /label="Restriction Enzyme"  
     misc_feature    5161..5833 
                     /label="ColE1"  
     misc_feature    6263..7057 
                     /label="Kan/Neo"  
     misc_feature    7092..7394 
                     /label="SV40 ori"  
     misc_feature    7480..7957 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4161..4167 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4161 
                     /label="Mical1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTCACCA ACCTCCACCA ACCCAGCACA TGACCACTTT GAGACCTTTG 
     1081 TGCAGGCACA GCTGTGCCAA GATGTACTGA GCAGTTTTCA GGGGCTCTGT AGAGCCCTGG 
     1141 GAGTGGAGTC TGGTGGGGGA TTACCCCAGT ACCACAAGAT CAAGGCCCAG CTCAACTACT 
     1201 GGAGTGCCAA GTCACTGTGG GCCAAGTTGG ACAAGAGAGC AAGCCAGCCT GCGTACCAGC 
     1261 AAGGCCAGGC CTGTACCAAC ACCAAGTGTC TCGTGGTAGG TGCTGGGCCT TGTGGACTTC 
     1321 GGGCTGCAGT GGAGCTTGCA CTGTTAGGTG CCCGAGTGGT GCTTGTGGAA AAGCGTACCA 
     1381 AGTTCTCTAG GCACAATGTA CTCCATCTCT GGCCCTTCAC CATCCATGAT CTCCGGGCAC 
     1441 TTGGGGCCAA GAAGTTCTAC GGGCGTTTCT GTACTGGGAC CCTGGACCAT ATCAGCATCC 
     1501 GACAGCTCCA GCTGCTTCTG CTGAAGGTGG CGTTACTGTT GGGGGTGGAG ATTCACTGGG 
     1561 GCTTCACTTT CACTGGCCTG CAGCCCCCTC CCAAAAAAGG GAGTGGCTGG CGTGCCCGGA 
     1621 TCCAGCCCAG TCCCCCAGCC CAACTGGCCA GCTACGAATT TGATGTCCTC ATCTCAGCTG 
     1681 GAGGAGGCAA ATTTGTCCCT GAAGGCTTCA CCATCAGAGA GATGCGCGGC AAACTGGCCA 
     1741 TCGGCATCAC AGCCAACTTT GTGAATGGGC GCACGGTGGA GGAGACACAG GTGCCAGAAA 
     1801 TCAGTGGCGT AGCTCGGATC TACAACCAAA AATTCTTCCA GAGCCTGCTC AAAGCCACAG 
     1861 GTATTGATCT GGAGAACATT GTGTACTACA AGGACGACAC CCACTACTTT GTCATGACAG 
     1921 CCAAGAAGCA GTGTCTGCTG AGGCTGGGGG TATTGCGCCA GGACTTGCCA GAGACTGATC 
     1981 AGCTGCTGGG CAAAGCTAAT GTGGTGCCTG AGGCTCTGCA GCAGTTTGCC AGAGCAGCGG 
     2041 CCGACTTCGC CACCCAAGGC AAGCTTGGGA AACTGGAGTT CGCTCAGGAT GCACGTGGGC 
     2101 GGCCCGATGT GGCAGCCTTC GACTTCACAA GCATGATGCG GTCAGAGAGT TCTGCTCGTA 
     2161 TCCAAGAAAA GCATGGTGCC CGCCTACTGC TGGGGCTGGT GGGGGACTGC CTTGTGGAGC 
     2221 CCTTCTGGCC TTTGGGCACT GGAGTGGCCC GAGGCTTCTT GGCAGCCTTC GATGCAGCCT 
     2281 GGATGGTGAA GCGGTGGGCG GAGGGTACTG GGCCTCTAGA GCTGTTGGCT GAACGTGAGA 
     2341 GCTTGTACCA GCTTCTGTCA CAAACATCCC CAGAGAATAT GCATCGAAAT GTAGCCCAGT 
     2401 ATGGGTTGGA CCCTGCCACC CGATACCCCA ACCTGAACCT CCGGGCTGTA ACGCCCAACC 
     2461 AGGTACAAGA CCTCTATGAC ATAATGGACA AGGAGCATGC TCGGAAGAAG AGTGACGAGA 
     2521 CGGATGCCCG GAAGACAACC ACAGGGTCTG CAGGCACTGA GGAGCTTCTG CACTGGTGCC 
     2581 AGGAGCAGAC AGCTGGCTTT CCTGGAGTCT CTGTCACTGA CTTCTCTTCC TCGTGGGCTG 
     2641 ATGGGCGAGC TCTGTGTGCC CTGGTACACC GCCTACAGCC TGGCCTTCTG GAACCCTCAG 
     2701 AGCTGCAGGG CATGAGCGCT CTGGAAGCCA CTGCCTGGGC ACTGAGGGTG GCAGAATATG 
     2761 AGTTGGGCAT CATACCAGTG TTGTCTGCAC AGGCCGTAGT GGCAGGCAGT GACCCACTGG 
     2821 GCCTCATTGC CTACCTCAGC CACTTCCACA GCGCCTTCAA GAACACACCC CACAGCTCAG 
     2881 GCCTTGTCAG CCAGCCTCAC GGGACCCCTA GTGCCATACT TTTCCTTGGC AAACTCCAGA 
     2941 GGAGCCTACA ACGGACCCGG ACCAAGGTAG AGGAGGAGAC TCCGTGTACT GAGGAGCCTC 
     3001 CTGTCTCTGA GCCCAGTGTG CCCCCAGCTC TGCCCTCCGA ACATGAGGAG GCTGGGGCAG 
     3061 AGGATGTGTG TGAGCTCTGT GGGAAACGCC TCTACATCCT GGAACGCTTC TGTGTGGATG 
     3121 GCCATTTCTT CCACCGCGGC TGCTTCTGCT GCCGTACCTG TGAGGCCACA TTGCGGCCAG 
     3181 GTGGCTATGG GCAATATCCA GGAGATGGAT ATTTCTACTG TCTCCAGCAC CTACCCCAGG 
     3241 AGGATCAGAA GGAGGCAGAC AACAATGGAA GTCCAGAGAA CCAGGAGCTC CCAACACCAG 
     3301 GAGACAGTAC CACACAGTCA GGCCCCTCCT CTCCTGTACC TCCTGTGACG GAGGCCAGCC 
     3361 CTGTCCCAAG CCCCAGCCAA CCTGCACGTC GGCTGATCCG CCTCTCCAGC GTAGAACGCC 
     3421 TACGGCTATC CTCCCTGAAC ATCATCCCTG ACTCAGGAGT GGAGCCTCCA CCCAAGCCCC 
     3481 CACGGAGCTG CTTGGACTTG GCCCAAGAGT CCCTCAAGAG CAGCTTTATG GGCTGGGGTG 
     3541 TGCTCCGGGC ACCACAGGTT CCTGAGGCCA TAGAGAAGGG GGAGGAGGAA GAAGAAGAGG 
     3601 AGGAGGAAGA AGAAGAGGAG GAGGAGGAGC TTCCGCCACC TTTGGCGCTA GAAGTGGAAC 
     3661 AGTCACTGCT GACCTTGGCC AAGAACTCAG GCGACATGAC TAAGTACCCA ACGTGGCGTC 
     3721 GAACCCTCAT GCGCCGTGCC AAAGAGGAGG AGATGAAGAG GTTCTGCAAG GCCCAGGCCA 
     3781 TCCAGCGGAG ACTAAACGAG ATTGAGGCTG CTATGAGGGA ACTGGAGACC GAAGGCATGA 
     3841 AGCTGGAGGT GGCCTTGAGG AAAGAGAGTA GCTCTCCAGA AAAGCAGAAG AAACTCTGGC 
     3901 TAGAGCAGCT GCTACAGCTC ATTCAGAAGA AGAACAGCCT AGTGACTGAG GAAGCCGAAC 
     3961 TCATGATCAC GGTGCAGGAG CTGGACCTGG AAGAGAAGCA GCGGCAACTG GACCACGAGT 
     4021 TTCGGGGCAT CAACCGGGAA GAAACTCTGA AGACCCAGGC CGACCGACTG TCTGAGGACC 
     4081 GGGTCCTAAG GAAGCTGTTG GATGTGGTGA ACCAGCGGGA TGCTCTGATC CAATTCCAGG 
     4141 AGGAGCGAAG GCTCAGAGAG ATGCCCGTAA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4201 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4261 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4321 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4381 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4441 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4501 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4561 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4621 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4681 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4741 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4801 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4861 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4921 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4981 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5041 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5101 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5161 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5221 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5281 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5341 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5401 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5461 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5521 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5581 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5641 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5701 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5761 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5821 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5881 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5941 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6001 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6061 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6121 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6181 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6241 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6301 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6361 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6421 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6481 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6541 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6601 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6661 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6721 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6781 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6841 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6901 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6961 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7021 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7081 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7141 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7201 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7261 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7321 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7381 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7441 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7501 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7561 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7621 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7681 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7741 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7801 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7861 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7921 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7981 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
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