LOCUS       RR205912                9357 bp    DNA     circular  SYN 
DEFINITION  Atp7a (Myc-DDK-tagged ORF) - Rat ATPase, Cu++ transporting, alpha polypeptide (Atp7a), (10 ug)
ACCESSION   NM_052803
VERSION     NM_052803.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5514..5605 
                     /label="Myc-DDK"  
     misc_feature    5635..6220 
                     /label="PolyA signal"  
     misc_feature    5692..5711 
                     /label="Restriction Enzyme"  
     misc_feature    6496..7168 
                     /label="ColE1"  
     misc_feature    7598..8392 
                     /label="Kan/Neo"  
     misc_feature    8427..8729 
                     /label="SV40 ori"  
     misc_feature    8815..9292 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5496..5502 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5496 
                     /label="Atp7a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAACCAAAT ATGGATGCAA ATTCAATTAC TATCACTGTT GAGGGAATGA 
     1081 CATGTATTTC CTGTGTCCGG ACCATTGAGC AGCAGATTGG GAAAGTGAAT GGTGTCCATC 
     1141 ACATTAAAGT TTCCCTAGAA GAAAAAAGTG CAACTGTTAT TTATAACCCT AAACTTCAGA 
     1201 CTCCAAAGAC CCTCCAAGAA GCTATCGATG ACATGGGCTT TGATGCTCTT CTTCACAATG 
     1261 CTAACCCTCT TCCTGTCTTA ACCAATACTG TGTTTCTGAC TGTTACTGCT CCACTGGCTC 
     1321 TGCCATGGGA CCATATCCAA AGTACATTGC TCAAGACCAA GGGTGTGACT GGGGTTAAGA 
     1381 TTTCCCCTCA GCAAAGAAGT GCAGTGGTTA CCATAATCCC ATCTGTGGTG AGTGCTAATC 
     1441 AGATCGTGGA GCTGGTCCCA GACCTCAGTT TAGACATGGG AACTCAGGAG AAAAAGTCAG 
     1501 GAACTTCTGA AGAGCATAGC ACACCTCAGG CAGGGGAAGT CCTGCTGAAG ATGAGAGTGG 
     1561 AAGGGATGAC CTGCCATTCA TGCACTAGCA CCATTGAAGG AAAAGTTGGA AAGCTGCAAG 
     1621 GTGTACAACG CATTAAAGTG TCCCTAGACA ACCAAGAAGC TACTATTGTG TATCAACCTC 
     1681 ATCTGATCAC AGCAGAGGAA ATAAAGAAGC AGATTGAAGC TGTGGGTTTT CCAGCCTTCA 
     1741 TAAAAAAACA GCCAAAGTAC CTCAAATTGG GAGCCATTGA CGTTGAGCGC CTGAAGAGTA 
     1801 CACCAGTCAA ATCTTCAGAA GGATCTCAGC AAAAGAGCCC AGCGTATCCC AGTGACTCAG 
     1861 CAATCACATT TACCATAGAC GGCATGCATT GTAAATCATG TGTGTCAAAT ATCGAAAGTG 
     1921 CTTTATCTAC ACTCCAGTAT GTAAGCAGCA TAGTAGTTTC TTTAGAGAAT AGGTCAGCCA 
     1981 TTGTAAAGTA CAATGCAAGC TTAGTCACTC CAGAAATACT GAGAAAAGCG ATAGAGGCCG 
     2041 TTTCTCCAGG GCAATACAGA GTTAGTATTT CAAGTGAAGT TGAAAGTCCC ACCAGCTCTC 
     2101 CCTCCAGCTC ATCTCTTCAG AAGATGCCTT TGAACTTAGT GAGCCAGCCT CTGACCCAAG 
     2161 AAGTTGTAAT CAACATTAAT GGCATGACTT GTAATTCTTG TGTGCAGTCG ATAGAGGGTG 
     2221 TAATATCGAA GAAGCCAGGT GTAAAATCCA TCCACGTTTC CCTCACAAAT AGCACCGGGA 
     2281 CTATTGAATA TGATCCTCTA CTAACCTCTC CAGAACCCTT GCGAGAAGCA ATTGAAGACA 
     2341 TGGGATTTGA TGCTGTCCTT CCAGCAGACA TGAAAGAGCC ACTGGTCGTG ATAGCTCAGC 
     2401 CCTCACTGGA AACACCTCTT TTGCCCTCAA CTACTGAGCC AGAAAATGTG ATGACACCAG 
     2461 TTCAGAACAA GTGTTACATA CAGGTCTCGG GGATGACGTG TGCTTCTTGT GTAGCAAACA 
     2521 TTGAACGGAA CTTAAGACGA GAAGAAGGAA TATATTCTGT ACTTGTAGCC CTAATGGCTG 
     2581 GCAAGGCGGA AGTAAGATAT AACCCAGCTG TTATCCAACC CCGAGTGATA GCAGAGCTTA 
     2641 TTCGAGAGCT TGGCTTTGGA GCTGTGGTGA TGGAAAATGC TGGAGAAGGC AATGGCATTT 
     2701 TGGAACTTGT TGTGAGAGGA ATGACGTGTG CCTCCTGTGT CCATAAAATT GAATCAACGC 
     2761 TCACAAAACA CAAAGGGATC TTCTACTGCT CTGTGGCCCT GGCAACGAAC AAAGCACATA 
     2821 TTAAATATGA TCCAGAAATT ATTGGTCCCA GAGATATTAT CCATACCATT GGAAACTTAG 
     2881 GTTTTGAAGC TTCTTTGGTC AAGAAAGATC GATCAGCCAA CCACTTAGAT CATAAACGAG 
     2941 AAATAAAACA ATGGAGAGGG TCTTTCCTTG TGAGCCTGTT TTTCTGTATC CCTGTAATGG 
     3001 GACTGATGAT CTATATGATG GTTATGGACC ACCACCTTGC AACTCTAAAC CATAATCAGA 
     3061 ACATGAGTAA TGAAGAAATG ATCAACATGC ATTCTTCTAT GTTCCTGGAG CGTCAGATCC 
     3121 TGCCGGGACT GTCCATTATG AATTTGTTGT CCCTTTTATT GTGTCTACCT GTACAGTTTT 
     3181 GTGGAGGCTG GTACTTTTAC ATTCAGGCTT ACAAAGCGCT GAGGCATAAG ACAGCAAATA 
     3241 TGGATGTGCT GATTGTGTTG GCAACCACCA TTGCATTTGC CTACTCTCTG GTCATTCTTC 
     3301 TGGTTGCAAT GTATGAGAGA GCCAAAGTGA ACCCTATTAC CTTCTTTGAT ACACCTCCTA 
     3361 TGCTGTTTGT GTTTATCGCA CTAGGCCGAT GGCTGGAACA CATAGCGAAG GGCAAAACTT 
     3421 CCGAGGCTCT TGCAAAGCTA ATTTCCTTAC AAGCAACAGA AGCAACTATT GTAACTCTTA 
     3481 ACTCTGAAAA TCTCCTCCTG AGTGAAGAAC AAGTGGATGT GGAACTTGTA CAACGTGGAG 
     3541 ATATCATTAA GGTCGTTCCA GGAGGCAAAT TTCCAGTGGA TGGCCGTGTT ATTGAAGGGC 
     3601 ATTCTATGGT AGACGAGTCC CTCATCACAG GGGAAGCAAT GCCTGTGGCT AAGAAACCTG 
     3661 GCAGCACGGT GATTGCAGGT TCCATTAACC AGAATGGATC GCTCCTCATC CGGGCAACAC 
     3721 ACGTAGGGGC AGATACAACA CTTTCTCAAA TTGTCAAACT TGTAGAGGAG GCACAGACAT 
     3781 CAAAGGCTCC TATCCAGCAG TTTGCAGACA AACTCAGTGG CTACTTTGTT CCTTTTATCG 
     3841 TCTTGGTTTC CATTGTTACC CTCTTGGTGT GGATTATAAT TGGATTTCAA AATTTTGAAA 
     3901 TTGTGGAAGC CTACTTTCCC GGCTACAACA GAAGCATCTC CCGAACAGAA ACCATAATCC 
     3961 GCTTTGCTTT CCAAGCGTCT ATCACAGTTC TGTGTATCGC ATGTCCCTGT TCACTGGGGC 
     4021 TAGCCACTCC AACTGCTGTG ATGGTGGGCA CAGGAGTAGG TGCTCAGAAT GGCATACTTA 
     4081 TCAAAGGTGG GGAGCCACTG GAGATGGCTC ATAAGGTAAA GGTAGTGGTG TTTGACAAGA 
     4141 CTGGAACCAT TACCCATGGA ACCCCAGTAG TGAACCAAGT AAAGGTTCTG GTGGAAAGTA 
     4201 ACAAGATATC ACGCAATAAG ATCCTGGCCA TTGTGGGAAC TGCAGAAAGT AACAGTGAAC 
     4261 ATCCTTTAGG AGCAGCTGTA ACCAAATATT GCAAGCAGGA GCTGGACACT GAAACCTTGG 
     4321 GTACCTGCAC AGATTTCCAG GTTGTACCCG GCTGTGGAAT TAGCTGTAAA GTCACCAATA 
     4381 TTGAAGGTTT GCTACATAAG AGTAACTTGA AGATAGAAGA AAATAACATT AAAAATGCAT 
     4441 CCCTGGTTCA AATTGATGCA ATTAATGAAC AGTCATCACC TTCATCGTCT ATGATTATTG 
     4501 ATGCTCATCT CTCAAATGCT GTTAATACTC AGCAGTACAA AGTCCTCATT GGTAACCGGG 
     4561 AATGGATGAT TAGAAATGGT CTTGTCATAA GTAATGATGT GGACGAGTCT ATGATTGAAC 
     4621 ATGAAAGAAG AGGCCGGACT GCTGTGTTGG TGACAATCGA TGATGAACTG TGTGGCTTGA 
     4681 TCGCTATTGC TGATACTGTG AAACCTGAGG CAGAGCTGGC TGTGCACATT CTGAAATCTA 
     4741 TGGGTTTGGA AGTAGTTCTG ATGACTGGAG ACAACAGTAA AACAGCTCGG TCTATTGCTT 
     4801 CTCAGGTTGG CATTACTAAG GTGTTTGCTG AAGTTCTGCC TTCCCACAAA GTTGCTAAGG 
     4861 TGAAGCAGCT TCAGGAGGAG GGAAAGCGCG TAGCCATGGT AGGAGATGGA ATCAATGACT 
     4921 CTCCAGCTCT GGCAATGGCA AGCGTCGGAA TTGCCATCGG CACAGGCACA GATGTAGCCA 
     4981 TCGAAGCAGC TGACGTGGTT TTGATAAGGA ATGACCTTCT GGATGTTGTG GCAAGTATTG 
     5041 ACTTGTCAAG GAAAACAGTC AAGAGGATTC GAATCAATTT TGTCTTTGCT CTGATTTATA 
     5101 ATCTGATTGG AATTCCCATC GCTGCTGGAG TTTTTCTGCC CATCGGCTTG GTTTTACAAC 
     5161 CCTGGATGGG ATCCGCAGCC ATGGCCGCTT CATCTGTCTC TGTGGTACTT TCTTCCCTTT 
     5221 TCCTCAAGCT TTACAGGAAG CCAACATATG ACAATTATGA GTTGCGTCCC CGGAGCCACA 
     5281 CAGGACAGAG GAGTCCTTCA GAAATCAGCG TTCACGTTGG AATAGATGAT ACCTCCAGAA 
     5341 ATTCTCCAAG ACTGGGTTTA CTGGACCGGA TTGTCAATTA CAGCAGAGCC TCCATAAATT 
     5401 CACTGCTGTC TGACAAACGC TCCCTCAACA GCGTCGTCAC TAGTGAGCCT GATAAGCACT 
     5461 CACTTCTGGT GGGAGACTTC CGGGAAGATG ACGACACCAC ACTGACGCGT ACGCGGCCGC 
     5521 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5581 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5641 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5701 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5761 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5821 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5881 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5941 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     6001 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     6061 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     6121 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     6181 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     6241 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     6301 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     6361 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     6421 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     6481 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6541 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6601 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6661 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6721 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6781 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6841 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6901 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6961 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     7021 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     7081 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     7141 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     7201 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     7261 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     7321 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     7381 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     7441 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     7501 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7561 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7621 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7681 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7741 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7801 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7861 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7921 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7981 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     8041 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     8101 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     8161 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     8221 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     8281 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     8341 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     8401 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     8461 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     8521 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8581 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8641 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8701 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8761 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8821 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8881 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8941 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     9001 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     9061 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     9121 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     9181 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     9241 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     9301 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//