LOCUS       RR205902                7962 bp    DNA     circular  SYN 
DEFINITION  Kif5a (Myc-DDK-tagged ORF) - Rat kinesin family member 5A (Kif5a), (10 ug)
ACCESSION   NM_212523
VERSION     NM_212523.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4119..4210 
                     /label="Myc-DDK"  
     misc_feature    4240..4825 
                     /label="PolyA signal"  
     misc_feature    4297..4316 
                     /label="Restriction Enzyme"  
     misc_feature    5101..5773 
                     /label="ColE1"  
     misc_feature    6203..6997 
                     /label="Kan/Neo"  
     misc_feature    7032..7334 
                     /label="SV40 ori"  
     misc_feature    7420..7897 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4101..4107 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4101 
                     /label="Kif5a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGACC AATAACGAAT GCAGCATCAA GGTGCTTTGC CGATTTCGGC 
     1081 CCCTGAACCA GGCCGAGATC CTGCGGGGGG ACAAGTTCAT CCCCATTTTC CAAGGGGACG 
     1141 ACAGCGTCAT TATTGGGGGA AAGCCATATG TCTTCGACCG TGTATTCCCC CCAAACACGA 
     1201 CTCAGGAGCA GGTTTACCAC GCCTGTGCCA TGCAGATCGT CAAAGACGTC CTTGCTGGCT 
     1261 ACAACGGCAC AATTTTTGCT TATGGACAGA CATCCTCAGG GAAAACGCAC ACCATGGAGG 
     1321 GAAAGCTGCA CGACCCTCAG CTCATGGGCA TCATTCCCCG GATCGCCCGA GACATCTTCA 
     1381 ACCACATCTA CTCCATGGAT GAGAACCTTG AGTTCCACAT TAAGGTGTCT TACTTTGAGA 
     1441 TTTACCTGGA TAAGATCCGT GACCTTTTGG ATGTGACCAA GACAAACCTG TCTGTGCATG 
     1501 AGGACAAAAA CCGGGTGCCG TTTGTCAGGG GTTGTACCGA ACGCTTTGTG TCCAGCCCAG 
     1561 AGGAGATTCT GGATGTGATT GATGAGGGGA AGTCGAACCG TCACGTTGCC GTCACCAACA 
     1621 TGAATGAGCA CAGCTCTCGG AGTCACAGCA TCTTCCTCAT CAACATCAAG CAGGAGAACA 
     1681 TAGAGACGGA GCAGAAGCTC AGCGGGAAAC TGTACCTTGC GGATCTGGCC GGAAGCGAGA 
     1741 AGGTCAGCAA GACAGGAGCA GAGGGAGCCG TTCTGGATGA GGCAAAGAAT ATCAATAAGT 
     1801 CGCTGTCGGC CCTGGGGAAT GTGATCTCTG CTCTGGCCGA GGGCACTAAA AGCTACGTGC 
     1861 CGTACCGTGA CAGCAAAATG ACGAGGATTC TCCAGGACTC TCTGGGAGGG AACTGCAGGA 
     1921 CCACCATGTT CATCTGCTGC TCACCATCCA GCTACAATGA CGCAGAGACC AAGTCCACAC 
     1981 TCATGTTTGG GCAGCGGGCA AAGACCATCA AGAACACTGC CTCAGTGAAT CTGGAGTTGA 
     2041 CTGCTGAGCA ATGGAAGAAG AAATATGAGA AGGAGAAGGA GAAGACCAAG GCCCAGAAGG 
     2101 AGACAATTGC GAAGCTGGAG GCTGAGCTTA GCCGGTGGCG CAATGGAGAG AATGTGCCTG 
     2161 AGACTGAGCG CCTGGCTGGA GAGGACTCAG CTCTGGCAGC CGAGATCTGT GAGGAGACCC 
     2221 CTGTGAATGA CAACTCATCC ATCGTGGTAC GCATCGCACC GGAGGAAAGG CAGAAATACG 
     2281 AGGAAGAGAT CCGCCGTCTC TACAAGCAGC TCGACGACAA GGATGATGAG ATCAACCAGC 
     2341 AGAGCCAACT CATTGAGAAG CTGAAGCAGC AGATGCTGGA CCAGGAAGAG CTGCTAGTGT 
     2401 CCACTCGGGG AGACAATGAG AAGGTCCAGC GGGAGCTGAG CCACCTGCAG TCTGAGAACG 
     2461 ATGCCGCGAA GGAGGAGGTG AAGGAAGTGT TGCAGGCCCT GGAGGAGCTG GCAGTCAACT 
     2521 ATGACCAGAA GTCCCAGGAG GTGGAGGAGA AGAGCCAGCA GAACCAGCTG CTGGTGGATG 
     2581 AGCTGTCCCA GAAAGTGGCC ACCATGCTGT CCCTGGAGTC TGAGCCACAG CGGCTCCAGG 
     2641 AAGTCAGTGG ACACCAGCGA AAACGGATCG CTGAGGTGCT GAATGGACTG ATGAAGGACC 
     2701 TGAGTGAGTT CAGTGTCATC GTGGGCAACG GCGAGATTAA GCTGCCTGTG GAGATCAGTG 
     2761 GGGCCATCGA AGAGGAGTTC ACGGTGGCCC GACTCTACAT CAGCAAGATC AAGTCAGAGG 
     2821 TGAAGTCCGT GGTTAAGCGA TGCCGGCAGC TGGAGAACCT CCAAGTGGAG TGTCATCGCA 
     2881 AGATGGAGGT GACCGGTAGG GAGCTGTCGT CTTGCCAGCT GCTCATCTCA CAGCACGAGG 
     2941 CCAAGATCCG TTCGCTCACG GAATACATGC AGACTGTGGA GTTGAAGAAG CGGCACCTGG 
     3001 AAGAGTCCTA TGACTCCCTG AGCGATGAGC TAGCCAAGCT CCAGGCGCAG GAAACTGTAC 
     3061 ACGAGGTAGC TCTGAAAGAC AAGGAGCCAG ATACGCAGGA TGCGGAGGAG GTGAAGAAGG 
     3121 CCCTGGAACT ACAGATGGAG AATCATCGTG AGGCCCATCA CCGGCAGCTG GCCCGCCTCC 
     3181 GAGATGAGAT CAATGAGAAA CAGAAGACCA TTGATGAGCT TAAAGACCTG GACCAGAAGC 
     3241 TCCAGTTAGA GCTGGAGAAG CTTCAGGCTG ATTACGAGAG ACTGAAGAAC GAAGAGAACG 
     3301 AGAAGAGTGC CAAGCTCCAG GAGCTGACAT TTCTGTATGA GCGGCATGAG CAGTCCAAGC 
     3361 AGGACCTCAA GGGGCTGGAG GAGACAGTTG CCCGTGAACT CCAGACCCTC CACAACCTGC 
     3421 GCAAGCTGTT CGTTCAAGAC GTCACGACTC GAGTCAAGAA AAGTGCAGAA ATGGAGCCCG 
     3481 AGGACAGTGG GGGGATTCAT TCCCAAAAGC AGAAGATCTC CTTTCTTGAG AACAACCTGG 
     3541 AACAGCTTAC AGAGGTTCAC AAACAGCTGG TACGTGACAA TGCAGATCTG CGTTGTGAGC 
     3601 TTCCTAAATT GGAAAAACGA CTTCGGGCTA CGGCTGAGAG AGTTAAGGCC CTGGAGGGTG 
     3661 CACTGAAGGA GGCCAAGGAG GGCGCTATGA AGGACAAGCG CCGATACCAG CAGGAGGTGG 
     3721 ACCGCATCAA GGAAGCTGTG CGGTACAAGA GCTCTGGCAA GCGGGGCCAC TCTGCCCAGA 
     3781 TTGCTAAGCC TGTGAGGCCC GGCCACTATC CTGCCTCCTC ACCCACCAAC CCCTACGGTA 
     3841 CCCGGAGTCC CGAGTGTATC AGCTACACCA ACAACCTCTT CCAGAACTAC CAGAACCTGC 
     3901 ACCTGCAGGC TGCGCCTAGC TCCACCTCAG ACGTGTACTT TGCCAGCAAC GGAGCCACAT 
     3961 CTGTTGCCCC CTTGGCTTCC TACCAGAAGG CCAACACGGA CAATGGAAAC GCCACAGATA 
     4021 TCAACGACAA CAGGAGTGAC CTGCCCTGCG GCTATGAGGC TGAGGACCCA GCCAAGCTTT 
     4081 TCCCTCTCCA CCAAGAGACG GCAGCCAGCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4141 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4201 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4261 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4321 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4381 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4441 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4501 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4561 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4621 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4681 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4741 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4801 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4861 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4921 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4981 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5041 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5101 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5161 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5221 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5281 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5341 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5401 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5461 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5521 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5581 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5641 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5701 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5761 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5821 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5881 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5941 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6001 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6061 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6121 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6181 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6241 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6301 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6361 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6421 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6481 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6541 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6601 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6661 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6721 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6781 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6841 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6901 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6961 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7021 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7081 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7141 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7201 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7261 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7321 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7381 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7441 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7501 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7561 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7621 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7681 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7741 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7801 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7861 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7921 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//