LOCUS       RR205866                7254 bp    DNA     circular  SYN 
DEFINITION  Sf3a1 (Myc-DDK-tagged ORF) - Rat splicing factor 3a, subunit 1 (Sf3a1), (10 ug)
ACCESSION   NM_001107235
VERSION     NM_001107235.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3411..3502 
                     /label="Myc-DDK"  
     misc_feature    3532..4117 
                     /label="PolyA signal"  
     misc_feature    3589..3608 
                     /label="Restriction Enzyme"  
     misc_feature    4393..5065 
                     /label="ColE1"  
     misc_feature    5495..6289 
                     /label="Kan/Neo"  
     misc_feature    6324..6626 
                     /label="SV40 ori"  
     misc_feature    6712..7189 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3393..3399 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3393 
                     /label="Sf3a1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGGCCGGA CCCGTGCAGG CGGTACCCCC GCCGCCGCCA GTAGTCACTG 
     1081 AGCCCAAACA GCCAAATGAA GAAGAAGCAT CATCAAAGGA GGATTCCACC CCTTCCAAGC 
     1141 CAGTGGTGGG GATTATTTAC CCTCCTCCAG AGGTCAGGAA TATCGTCGAC AAGACCGCCA 
     1201 GTTTTGTGGC CAGAAATGGG CCTGAGTTTG AAGCTAGGAT TCGACAGAAT GAGATCAACA 
     1261 ACCCCAAGTT CAATTTCTTA AATCCCAATG ACCCTTACCA TGCCTACTAC CGCCACAAGG 
     1321 TCAGCGAGTT CAAGGAGGGG AAGGCTCAGG AACCCTCAGC TGCCATCCCC AAGGTTATGC 
     1381 AGCAGCAGCA GCAAGCCACA CAACAACAGC TGCCCCAGAA GGTCCAAGCC CAGGTGATCC 
     1441 AAGAGACCAT AGTACCGAAG GAGCCCCCTC CTGAGTTCGA GTTCATCGCT GACCCCCCCT 
     1501 CCATCTCAGC CTTCGACCTG GATGTAGTGA AGCTGACAGC TCAGTTTGTG GCCAGGAATG 
     1561 GGCGTCAGTT TCTGACCCAG CTGATGCAGA AAGAACAGCG CAACTACCAG TTTGACTTTC 
     1621 TCCGACCCCA GCACAGCCTC TTCAATTACT TCACAAAGCT GGTGGAGCAG TATACCAAGA 
     1681 TCTTGATTCC ACCCAAAGGC TTATTTGCAA AGCTCAAGAA AGAGGCTGAA AATCCCCGAG 
     1741 AAGTTTTGGA CCAGGTTTGT TACCGAGTAG AATGGGCCAA ATTTCAGGAA CGTGAGAGGA 
     1801 AGAAGGAGGA AGAAGAAAAA GAGAAGGAGC GAGTAGCCTA TGCTCAGATC GACTGGCATG 
     1861 ACTTTGTGGT AGTGGAGACA GTGGATTTTC AACCCAATGA GCAAGGGAAC TTTCCACCCC 
     1921 CAACAACACC GGAGGAACTG GGAGCTCGGA TCCTCATTCA GGAACGCTAC GAGAAGTTTG 
     1981 GGGAAAGTGA GGAGGTGGAG ATGGAGGTTG AGTCTGATGA GGAAGATCAA GAAAAGACTG 
     2041 AGGAGACTCC TTCCCAGCTG GACCAGGATA CTCAAGTACA GGACATGGAT GAGGGTTCAG 
     2101 ACGATGAAGA AGAAGGACAG AAAGTGCCTC CACCCCCAGA GACACCCATG CCTCCACCTC 
     2161 TGCCCCCAAC ACCAGACCAA GTCATTGTGC GAAAGGACTA TGATCCCAAA GCTTCCAAGC 
     2221 CCCTTCCTCC AGCTCCTGCT CCTGATGAGT ATCTTGTGTC CCCCATAACT GGGGAGAAGA 
     2281 TTCCTGCCAG CAAAATGCAA GAACACATGC GCATCGGGCT TCTTGACCCC CGCTGGCTGG 
     2341 AGCAACGGGA TCGCTCCATC CGAGAGAAGC AGAGCGATGA TGAGGTGTAC GCACCAGGTC 
     2401 TGGATATTGA GAGCAGCCTA AAGCAGTTGG CTGAGCGACG TACTGACATC TTTGGGGTAG 
     2461 AAGAGACAGC CATTGGTAAG AAGATTGGTG AAGAGGAGAT CCAGAAGCCA GAGGAAAAGG 
     2521 TGACTTGGGA TGGCCATTCA GGCAGCATGG CCCGGACCCA GCAGGCTGCC CAGGCCAACA 
     2581 TCACCCTCCA GGAGCAGATT GAGGCCATCC ATAAGGCCAA GGGCCTGGTG CCAGAAGATG 
     2641 ACACCAAAGA GAAGATTGGC CCTAGCAAGC CCAATGAAAT CCCTCAGCAG CCACCGCCTC 
     2701 CATCTTCAGC CACCAATATC CCCAGCTCAG CGCCCCCCAT CACGTCAGTT CCCCGGCCAC 
     2761 CTGCGATGCC ACCTCCAGTC CGCACCACTG TCGTATCTGC AGTGCCTGTC ATGCCTCGGC 
     2821 CCCCCATGGC ATCTGTGGTC CGGCTGCCCC CAGGCTCAGT GATTGCCCCG ATGCCACCCA 
     2881 TCATCCACGC ACCTAGGATC AACGTGGTAC CCATGCCTCC TGCAGCACCT CCCATCATGG 
     2941 CACCCCGCCC ACCTCCCATG ATTGTGCCAA CAGCCTTTGT GCCTGCTCCC CCTGTGGCTC 
     3001 CAGTGCCAGC CCCAGCTCCA ATGCCTCCCG TACACCCCCC ACCTCCTATG GAAGATGAGC 
     3061 CTCCATCCAA GAAACTAAAG ACCGAGGACA GCCTCATGCC TGAGGAAGAG TTCCTGCGCA 
     3121 GGAACAAGGG TCCAGTATCC ATCAAGGTGC AGGTACCCAA CATGCAGGAT AAGACAGAAT 
     3181 GGAAGCTGAA TGGGCAGGGG CTGGTCTTCA CTCTCCCGCT CACAGACCAG GTCTCTGTCA 
     3241 TCAAAGTGAA GATTCATGAA GCCACGGGCA TGCCTGCAGG GAAGCAGAAA CTACAGTATG 
     3301 AAGGCATCTT CATCAAGGAC TCCAACTCCC TGGCTTACTA CAACATGGCC AGTGGTGCTG 
     3361 TCATCCACTT GGCCCTCAAG GAAAGAGGCG GCCGGAAGAA GACGCGTACG CGGCCGCTCG 
     3421 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3481 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3541 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3601 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     3661 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     3721 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     3781 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     3841 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     3901 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     3961 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4021 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4081 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4141 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4201 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4261 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4321 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4381 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4441 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4501 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4561 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4621 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     4681 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     4741 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     4801 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     4861 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     4921 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     4981 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5041 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5101 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5161 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5221 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5281 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5341 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5401 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5461 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5521 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     5581 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     5641 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     5701 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     5761 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     5821 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     5881 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     5941 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6001 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6061 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6121 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6181 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6241 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6301 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6361 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6421 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6481 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6541 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6601 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     6661 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     6721 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     6781 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     6841 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     6901 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     6961 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7021 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7081 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7141 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7201 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//