LOCUS       RR205838                7533 bp    DNA     circular  SYN 
DEFINITION  Erap1 (Myc-DDK-tagged ORF) - Rat endoplasmic reticulum aminopeptidase 1 (Erap1), (10 ug)
ACCESSION   NM_030836
VERSION     NM_030836.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3690..3781 
                     /label="Myc-DDK"  
     misc_feature    3811..4396 
                     /label="PolyA signal"  
     misc_feature    3868..3887 
                     /label="Restriction Enzyme"  
     misc_feature    4672..5344 
                     /label="ColE1"  
     misc_feature    5774..6568 
                     /label="Kan/Neo"  
     misc_feature    6603..6905 
                     /label="SV40 ori"  
     misc_feature    6991..7468 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3672..3678 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3672 
                     /label="Erap1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCTCTCTT CTTTCCCTAG TATTGACCTT TTTAGCTGTG TCTTCTCCTT 
     1081 CTTGTTGTCA GAACAGTGAC ACAGCATCTC CAAAAGCTAG TAATGGGGCC TCATTCCCTT 
     1141 GGAATAATAT GCGACTTCCT GAGTATATCA CCCCAATTCA TTATGATCTC ATGATCCATG 
     1201 CAAACCTTAG CACTCTGACT TTCTGGGGGA AAACAGAAGT AGAAATCACA GTTAGCCAGC 
     1261 CCACCAGCAC CATTATCATG CATAGTCACC AGCTGCAAAT ATCTAAGGCC ACCCTCAGGA 
     1321 GAGGAGCTGA AGAGATGCTG CCTGAAGAAC CACTGAAGCT CATGGAATAT TCTGCTCATG 
     1381 AGCAAGTTGC ACTGCTGACT GCCCAGCCGC TCCTTGCTGG GTCCGTGTAC ACAGTTATCA 
     1441 TCACCTATGC TGCCAACCTG TCTGAGAATT TCCATGGATT CTATAAAAGC ACCTACAGAA 
     1501 CACAGGAGGG GGAAAGGAGA ATACTTGCAG CAACACAGTT TGAACCCACA GCTGCTAGAA 
     1561 TGGCTTTTCC CTGCTTTGAT GAGCCTGCCC TCAAGGCAAG TTTTTCCATC AAGATAAAGA 
     1621 GGGATCCAAG GCACCTGGCC ATCTCCAACA TGCCCTTGGT GAAATCTGTG ACTGTTGCTG 
     1681 AAGGACTCAT AGAAGACCAT TTTGACATCA CTGTGAAAAT GAGCACCTAC CTTGTGGCCT 
     1741 TCATTATTTC TGATTTTAAG TCTGTGAGCA AGATGACTAA GAGTGGAGTC AAGGTCTCTG 
     1801 TGTATGCTGT GCCAGACAAG ATAAATCAAG CAGATTATGC TCTGGATGCT GCAGTGACTC 
     1861 TTCTAGAGTT TTATGAGGAT TATTTCAGCA TTCCATATCC TTTACCCAAG CAAGATCTTG 
     1921 CTGCAATTCC AGACTTTCAG TCTGGTGCTA TGGAAAACTG GGGCCTGACC ACATACAGAG 
     1981 AATCTGCTTT GTTATATGAT AAAGAAAAGT CTTCTGCATC CAGTAAGCTT GGCATCACAA 
     2041 TGACTGTATC CCATGAACTG GCTCACCAGT GGTTCGGGAA CTTGGTCACA ATGGAATGGT 
     2101 GGAATGACCT TTGGCTCAAT GAAGGATTTG CCAAATTTAT GGAGTTTGTG TCTGTCACTG 
     2161 TGACCCATCC TGAATTAAAA GTTGAAGAAT ATTTTTTTGG CAAGTGTTTT AATGCAATGG 
     2221 AAGTTGATGC ATTAAACTCC TCTCACCCTG TATCCACACC TGTGGAGAAT CCTGCACAGA 
     2281 TTCGGGAGAT GTTTGATGAG GTTTCTTATG AAAAGGGTGC TTGTATTCTG AATATGCTAA 
     2341 GAGATTATCT GAGTGCAGAT ACATTTAAAA GAGGAATTGT ACAGTATCTT CAAAAGTATA 
     2401 GTTATAAAAA CACGAAAAAT GAGGACCTGT GGAATAGCAT GATGCATATT TGTCCCACAG 
     2461 ATGGCACACA AACAATGGAT GGCTTTTGCT CTAGGAACCA ACACTCATCA TCAACCTCAC 
     2521 ACTGGCGTCA GGAGGTTATA GATATAAAGA GCATGATGAA CACATGGACA CTGCAGAAGG 
     2581 GCTTTCCTCT GATAACCATC ACTGTAAGAG GGCGGAATGT GCACTTGAAG CAAGAACACT 
     2641 ACATGAAAGG TTCTGAATGC TTCCCAGAGA CTGGGTCTCT GTGGCATGTT CCGCTGACAT 
     2701 TCATTACCAG CAAATCAGAT TCAGTCCAGA GATTTTTGCT GAAGACAAAG ACAGATGTGA 
     2761 TCATCCTCCC AGAAGCAGTG GAATGGATCA AATTTAATGT AGGAATGAAT GGCTATTACA 
     2821 TTGTGCATTA CGGGGATGAT GGATGGGCTT CTCTGAATGG CCTCTTAAAA GAAGCACATA 
     2881 CAACAATCAG CAGTAATGAT CGGGCAAGTC TCATCAACAA TGCATTTCAG CTGGTCAGCA 
     2941 TCGGAAAGTT ATCAATTGAA AAAGCCCTGG ATTTAATCCT GTACTTGAAA AATGAAACTG 
     3001 AAATTATGCC CATATTCCAA GGTTTGAATG AACTGATACC TATGTATAAG TTAATGGAGA 
     3061 AAAGAGATAT GGTTGAGGTG GAAACTCAAT TCAAGGACTT TCTCCTCAGG CTGCTAAAGG 
     3121 ACCTTATTAA TAAGCAGACC TGGACAGATG AAGGCTCTGT CTCAGAGAGG ATGCTCAGGA 
     3181 GCCAGCTCTT ACTCCTAGCA TGTGTGCACA GGTATCAGCT CTGCGTGCAG AGGGCAGAGC 
     3241 GCTATTTCAG AGAGTGGAAG GCATCCAATG GAAACATGAG CCTCCCTATT GATGTGACCT 
     3301 TGGCGGTGTT TGCTGTTGGG GCCCAGAACA CAGAAGGCTG GGATTTTCTT TATAGTAAAT 
     3361 ATCAGTCTTC TTTGTCTAGT ACCGAGAAAA GCCAAATTGA ATTTTCCCTC TGCATAAGCC 
     3421 AAGACCCAGA AAAACTTCAG TGGCTTCTAG ACCAAAGTTT TAAGGGAGAG ATAATAAAAA 
     3481 CTCAGGAATT CCCACATATT CTCACGCTCA TTGGCAGAAA CCCAGTGGGT TATCCATTGG 
     3541 CCTGGAAGTT TCTGAAGGAA AATTGGAACA AGATTGTACA GAAATTTGAA CTTGGCTCAT 
     3601 CATCCATAGC TCACATGGTA ATGGGAACAA CAAACCAGTT TTCCACCAGA GCACGTCTTG 
     3661 AAGAGGTAAA GGGGTGTATG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3721 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3781 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3841 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3901 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3961 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4021 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4081 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4141 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4201 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4261 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4321 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4381 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4441 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4501 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4561 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4621 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4681 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4741 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4801 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4861 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4921 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4981 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5041 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5101 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5161 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5221 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5281 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5341 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5401 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5461 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5521 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5581 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5641 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5701 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5761 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5821 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5881 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5941 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6001 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6061 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6121 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6181 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6241 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6301 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6361 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6421 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6481 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6541 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6601 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6661 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6721 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6781 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6841 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6901 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6961 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7021 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7081 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7141 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7201 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7261 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7321 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7381 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7441 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7501 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//