LOCUS       RR205835                8952 bp    DNA     circular  SYN 
DEFINITION  Usp19 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 19 (Usp19), (10 ug)
ACCESSION   NM_001001516
VERSION     NM_001001516.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5109..5200 
                     /label="Myc-DDK"  
     misc_feature    5230..5815 
                     /label="PolyA signal"  
     misc_feature    5287..5306 
                     /label="Restriction Enzyme"  
     misc_feature    6091..6763 
                     /label="ColE1"  
     misc_feature    7193..7987 
                     /label="Kan/Neo"  
     misc_feature    8022..8324 
                     /label="SV40 ori"  
     misc_feature    8410..8887 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5091..5097 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5091 
                     /label="Usp19"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTGCTGGC ACCAGTGCTA CAGGCCCAAG GAGGGGGCCT CCGGGATTGG 
     1081 AGGAGGCAAC TAGTAAGAAG AAACAGAAGG ATCGAGCAAA CCAGGAAAGT AAGGATGGAG 
     1141 ATCCTAGGAG AGTGTCCATG CCTCGAAAGG AGCCCACCAA AGATGAACTG TTGCTCGATT 
     1201 GGAGACAGAG TTCAGATAAG GTGGTTGTTA AGCTGCGCGT GGGAACAGGT CCCATATGTC 
     1261 TGGAGGAAGT AGATGCTGCT TTCACAGACA CGGACTGTGT GGTGAGGCTT CCAGATGGTC 
     1321 GGCAGTGGGG TGGTGTCTTC TTTGCTAAAA TACAAAGTTC TTGTACCAAA GTGCAGACTC 
     1381 GCAAGGGTGG TCTTCTACAG TTGGCACTAC CAAAGAAGGT GCCTCTTCTC ACGTGGCCCT 
     1441 CTCTCCTGAA GAAACCTCTA GGGACCCAAG AACTGGTGCC AGGATTGCGG TGCCAGGAGA 
     1501 ACGGACAAGA ACTGTCTCCC ATTGCCCTGG AGCCAGGTTC TGAGCCCCGC AGAGCTAAAC 
     1561 AGGAAGCTCG AAACCAGAAG AGGGCCCAGG GCCGTGGTGA GGTAGGCTCA GGGGCTAGCC 
     1621 CTGGGGCACA GGCAGGGCCC AGTGCCAAGA GGGCTGTTCA TCTCTGCAGA GGGCCAGAAG 
     1681 GTGAAGGGTC CATGGATGGG CCTGGCCCCC AGGGTGATGC CCCATCCTTC CTGTCCGACT 
     1741 CAGCTACGCA GGTTGAGGCT GAGGAGCAGC TCCATGTTCC ACCAGTGAAC CCTCAAACAA 
     1801 GTCTCTTGGG CTCAGAGAAG AATTTAGCCC TTTTGACAGT AGAGAAGACA GTGTCCCCCA 
     1861 GGAGTGACTC AGTCTCCCCA GTCATGATCC GGAACAGAGA CCCTGAGAAA GATGACCATT 
     1921 TCAAAGAGGA GATGGCAGTT GGAGCTGATC CTGCAGCCTT AGCGGATGAA CCAGAGTCTA 
     1981 TGGTGAACCT GGCATTTGTC AAGAATGATT CGTATGAGAA GGGGCCGGAT TCAGTGGTGG 
     2041 TGCACGTGTA TGTGAAGGAG AGCCGCAGGG ATACCTCTCG AGTACTTTTC CGAGAGCAGG 
     2101 ACTTCACGCT GATCTTCCAG ACCAGGGATG GAAATTTTCT GAGGCTGCAT CCGGGCTGTG 
     2161 GGCCCCACAC CATCTTCCGC TGGCAGGTGA AGCTCAGGAA CTTGATTGAG CCAGAGCAAT 
     2221 GTACGTTTTG TTTCACGGCC TCTCGAATCG ATATCTGCCT CCGGAAGCGG CAGAGTCAGC 
     2281 GCTGGGGGGG ACTGGAGGCC CCTGCTACAC GAGTGGGTGG TGCAAAGGTT GCCGTGCCGA 
     2341 CAGGTCCAAC CCCTTTGGAT TCAACCCCTC CAGGAGGTGG CCCCCTCCCT CTGACTGGCC 
     2401 AGGAGGAAGC CAGGGCTGTA GAGAAGGAAA AACCCAAGGC TCGGTCAGAG GACTCGGGGC 
     2461 TGGATGGTGT GGTGGCTCGC ACCCCCTTGG AGCATGTTAC CCCAAAGCCA GAACCGCACT 
     2521 TGGCCTCGCC CAAACCCACA TGTATGGTGC CTCCAATGCC TCATAGTCCA GTGAGTGGAG 
     2581 ATAGTGTGGA GGAGGATGAA GAGGAAGAGA AGAAGGTTTG TCTCCCAGGC TTTACTGGCC 
     2641 TCGTCAACTT AGGGAACACC TGCTTCATGA ATAGTGTCAT TCAGTCTCTG TCCAACACTC 
     2701 GGGAGCTTCG TGACTTCTTC CACGACCGAT CCTTTGAGGC TGAGATTAAC TACAATAACC 
     2761 CATTGGGGAC TGGTGGGCGT CTGGCTATTG GCTTCGCTGT GCTGCTCCGG GCCCTGTGGA 
     2821 AGGGCACTCA CCAAGCCTTT CAACCCTCCA AGCTAAAGGC CATTGTAGCA AGCAAGGCCA 
     2881 GCCAGTTTAC AGGCTACGCA CAGCATGATG CCCAAGAGTT CATGGCTTTC TTGTTGGATG 
     2941 GGCTGCATGA AGACCTGAAT CGGATCCAAA ACAAACCCTA CACAGAGACT GTGGACTCAG 
     3001 ATGGGCGACC TGACGAGGTG GTGGCTGAAG AAGCATGGCA GCGGCACAAG ATGAGGAACG 
     3061 ACTCATTCAT TGTGGACCTG TTTCAGGGCC AGTACAAGTC AAAGCTGGTG TGCCCTGTGT 
     3121 GTGCCAAGGT CTCCATCACT TTTGACCCGT TCCTTTATCT GCCGGTACCC TTGCCACAAA 
     3181 AGCAAAAGGT TCTCCCCATA TATTATTTTG CCAGAGAGCC CCATAGCAAA CCCATCAAGT 
     3241 TCCTGGTGAG CGTCAGCAAG GAGAACTCCA GCGCAAGCGA AGTTTTGGAG TCCCTCTCTC 
     3301 AGAGTGTCCA CGTGAAGCCT GAGAGCCTGC GCCTGGCTGA GGTAATTAAG AATCGTTTCC 
     3361 ACCGTGTTTT TTTGCCCTCC CACTCACTGG ATGCTGTGTC GCCAACGGAC GTGCTCCTGT 
     3421 GCTTTGAGCT GCTATCCCCA GAATTGGCTA AGGAGAGGGT AGTGGTGCTA GAGGTGCAGC 
     3481 AGCGCCCCCA GGTACCCAGC ATCCCTATCT CCAAGTGTGC AGCCTGCCAG CGGAAGCAGC 
     3541 AATCAGAAGA TGAAAAACTG AAGCGTTGTA CCCGTTGCTA CCGTGTGGGC TACTGCAACC 
     3601 AGTTCTGTCA GAAAACCCAT TGGCCTGACC ACAAAGGCCT CTGCCGCCCC GAGAACATTG 
     3661 GCTACCCCTT CCTGGTCAGT GTACCTGCTT CACGCCTCAC TTATGCCCGT CTTGCTCAGC 
     3721 TACTAGAAGG TTATGCCCGG TACTCTGTGA GCGTATTCCA ACCACCCTTC CAGCCTGGCC 
     3781 GAATGGCTTT GGAATCACAG AGCCCTGGCT GTACCACGTT GCTTTCAACC AGCTCTCTGG 
     3841 AGGCTGGGGA TAGTGAAAGA GAACCCATTC AGCCTTCTGA GCTCCAGTTG GTGACTCCTG 
     3901 TGGCTGAGGG GGATACCGGG GCTCACCGCA TGTGGCCACC TGCTGATCGG GGTCCTGTGC 
     3961 CTAGCACCAG TGGAATTTCT TCCGAGATGC TGGCCAGTGG GCCTATGGAA GGGTGTTCCT 
     4021 TGCTTGCTGG TGAGAGGGTG TCTCGGCCTG AAGCTGCTGT GCCTGGGTAC CAGCACTCAC 
     4081 GTGAGTCTGT GAGCGCCCAC ACGCCCCAGT TCTTCATCTA TAAAATTGAT GCGTCAAGCC 
     4141 GTGAGCAGCG GCTGGAGGAC AAAGGGGACA CTCCACTGGA GCTAGGTGAT GACTGTAGCC 
     4201 TGGCTCTGGT GTGGCGGAAC AACGAACGCC TTCAGGAGTT TGTGCTGGTG GCCTCCAAGG 
     4261 AGCTGGAGTG TGCTGAAGAC CCAGGCTCCG CTGGTGAGGC TGCCCGTGCT GGCCACTTCA 
     4321 CCCTGGACCA GTGCCTCAAT CTCTTTACAC GGCCTGAAGT GCTGGCACCT GAGGAAGCCT 
     4381 GGTACTGCCC ACAGTGCAAA CAGCATCGAG AGGCCTCCAA GCAGCTGCTG CTGTGGCGCC 
     4441 TACCAAATGT GCTCATCGTG CAGCTCAAGC GTTTCTCCTT TCGTAGCTTC ATTTGGCGTG 
     4501 ACAAGATCAA TGACTTGGTG GAGTTTCCAG TTCGGAACCT GGACTTGAGC AAGTTCTGTA 
     4561 TTGGTCAGAA AGAGGAGCAG CTGCCTAGCT ATGACCTGTA TGCAGTCATC AACCACTACG 
     4621 GAGGCATGAT CGGCGGCCAC TACACTGCCT GTGCACGGCT GCCCAGTGAC CGTAGTAGCC 
     4681 AGCGCAGTGA CGTGGGCTGG CGCTTGTTTG ATGACAGCAC GGTGACAACA GTAGACGAGA 
     4741 GCCAGGTGGT GACACGCTAT GCCTATGTGC TCTTCTACCG TCGGCGGAAC TCTCCTGTGG 
     4801 AGAGGCCCCC CAGGGCAGCT CACGCTGAAC ACCACCCAGA CCTAGGCCCT GCAGCCGAGG 
     4861 CTGCTGCCAG CCAGGCTTCC CGGATTTGGC AGGAGCTCGA GGCCGAGGAG GAGATGGTGC 
     4921 CCGAGGGGCC TGGGCCTCTG GGTCCTTGGG GGCCCCAAGA CTGGGTGGGG CCCCCGCCAC 
     4981 GTGGCCCTAC CACATCAGAC GAGGGCTGCC TCCGATACTT TGTCCTGGGT ACCGTGGCGG 
     5041 CTTTGGTGGC CCTTGTGCTC AACGTATTCT ATCCTCTGGT ATCTCAGAGT CGCTGGAGAA 
     5101 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     5161 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     5221 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     5281 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5341 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5401 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5461 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5521 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5581 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5641 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5701 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5761 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5821 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5881 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5941 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     6001 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     6061 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     6121 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     6181 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     6241 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6301 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6361 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6421 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6481 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6541 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6601 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6661 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6721 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6781 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6841 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6901 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6961 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     7021 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     7081 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     7141 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     7201 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     7261 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7321 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7381 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7441 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7501 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7561 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7621 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7681 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7741 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7801 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7861 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7921 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7981 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     8041 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     8101 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     8161 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     8221 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     8281 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8341 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8401 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8461 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8521 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8581 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8641 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8701 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8761 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8821 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8881 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8941 AACGCGAATT TT
//