LOCUS       RR205833                8658 bp    DNA     circular  SYN 
DEFINITION  Erbb2 (Myc-DDK-tagged ORF) - Rat v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (Erbb2), (10 ug)
ACCESSION   NM_017003
VERSION     NM_017003.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4815..4906 
                     /label="Myc-DDK"  
     misc_feature    4936..5521 
                     /label="PolyA signal"  
     misc_feature    4993..5012 
                     /label="Restriction Enzyme"  
     misc_feature    5797..6469 
                     /label="ColE1"  
     misc_feature    6899..7693 
                     /label="Kan/Neo"  
     misc_feature    7728..8030 
                     /label="SV40 ori"  
     misc_feature    8116..8593 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4797..4803 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4797 
                     /label="Erbb2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATCATCATG GAGCTGGCGG CCTGGTGCCG CTGGGGGTTC CTCCTCGCCC 
     1081 TCCTGCCCCC CGGAATCGCG GGCACCCAAG TGTGTACCGG CACAGACATG AAGTTGCGGC 
     1141 TCCCTGCCAG TCCTGAGACC CACCTGGACA TGCTCCGCCA CCTGTACCAG GGCTGTCAGG 
     1201 TAGTGCAGGG CAACTTGGAG CTTACCTACG TGCCTGCCAA TGCCAGCCTC TCATTCCTGC 
     1261 AGGACATCCA GGAAGTTCAG GGTTACATGC TCATCGCTCA CAACCAGGTG AAGCGCGTCC 
     1321 CACTGCAAAG GCTGCGCATC GTGAGAGGGA CCCAGCTCTT TGAGGACAAG TATGCCCTGG 
     1381 CTGTGCTAGA CAACCGAGAT CCTCAGGACA ATGTCGCCGC CTCCACCCCA GGCAGAACCC 
     1441 CAGAGGGGCT GCGGGAGCTG CAGCTTCGAA GTCTCACAGA GATCCTGAAG GGAGGAGTTT 
     1501 TGATCCGTGG GAACCCTCAG CTCTGCTACC AGGACATGGT TTTGTGGAAG GACGTCTTCC 
     1561 GCAAGAATAA CCAACTGGCT CCTGTCGATA TAGACACCAA TCGTTCCCGG GCCTGTCCAC 
     1621 CTTGTGCCCC CGCCTGCAAA GACAATCACT GTTGGGGTGA GAGTCCGGAA GACTGTCAGA 
     1681 TCTTGACTGG CACCATCTGT ACCAGTGGTT GTGCCCGGTG CAAGGGCCGG CTGCCCACTG 
     1741 ACTGCTGCCA TGAGCAGTGT GCCGCAGGCT GCACGGGCCC CAAGCATTCT GACTGCCTGG 
     1801 CCTGCCTCCA CTTCAATCAT AGTGGTATCT GTGAGCTGCA CTGCCCAGCC CTCGTCACCT 
     1861 ACAACACAGA CACCTTTGAG TCCATGCACA ACCCTGAGGG TCGCTACACC TTTGGTGCCA 
     1921 GCTGCGTGAC CACCTGCCCC TACAACTACC TGTCTACGGA AGTGGGATCC TGCACTCTGG 
     1981 TGTGTCCCCC GAATAACCAA GAGGTCACAG CTGAGGACGG AACACAGCGT TGTGAGAAAT 
     2041 GCAGCAAGCC CTGTGCTCGA GTGTGCTATG GTCTGGGCAT GGAGCACCTT CGAGGGGCGA 
     2101 GGGCCATCAC CAGTGACAAT GTCCAGGAGT TTGATGGCTG CAAGAAGATC TTTGGGAGCC 
     2161 TGGCATTTTT GCCGGAGAGC TTTGATGGGG ACCCCTCCTC CGGCATTGCT CCGCTGAGGC 
     2221 CTGAGCAGCT CCAAGTGTTC GAAACCCTGG AGGAGATCAC AGGTTACCTG TACATCTCAG 
     2281 CATGGCCAGA CAGTCTCCGT GACCTCAGTG TCTTCCAGAA CCTTCGAATC ATTCGGGGAC 
     2341 GGATTCTCCA CGATGGCGCG TACTCATTGA CACTGCAAGG CCTGGGGATC CACTCGCTGG 
     2401 GGCTGCGCTC ACTGCGGGAG CTGGGCAGTG GATTGGCTCT GATTCACCGC AACGCCCATC 
     2461 TCTGCTTTGT ACACACTGTA CCTTGGGACC AGCTCTTCCG GAACCCACAT CAGGCCCTGC 
     2521 TCCACAGTGG GAACCGGCCG GAAGAGGATT GTGGTCTCGA GGGCTTGGTC TGTAACTCAC 
     2581 TGTGTGCCCA CGGGCACTGC TGGGGGCCAG GGCCCACCCA GTGTGTCAAC TGCAGTCATT 
     2641 TCCTTCGGGG CCAGGAGTGT GTGGAGGAGT GCCGAGTATG GAAGGGGCTC CCCCGGGAGT 
     2701 ATGTGAGTGA CAAGCGCTGT CTGCCGTGTC ACCCCGAGTG TCAGCCTCAA AACAGCTCAG 
     2761 AGACCTGCTT TGGATCGGAG GCTGATCAGT GTGCAGCCTG CGCCCACTAC AAGGACTCGT 
     2821 CCTCCTGTGT GGCTCGCTGC CCCAGTGGTG TGAAACCGGA CCTCTCCTAC ATGCCCATCT 
     2881 GGAAGTACCC GGATGAGGAG GGCATATGCC AGCCGTGCCC CATCAACTGC ACCCACTCCT 
     2941 GTGTGGATCT GGATGAACGA GGCTGCCCAG CAGAGCAGAG AGCCAGCCCG GTGACATTCA 
     3001 TCATTGCAAC TGTAGTGGGC GTCCTGCTGT TCCTGATCTT AGTGGTGGTC GTTGGAATCC 
     3061 TAATCAAACG AAGGAGACAG AAGATCCGGA AGTATACGAT GCGTAGGCTG CTGCAGGAAA 
     3121 CTGAGTTAGT GGAGCCGCTG ACGCCCAGCG GAGCAATGCC CAACCAGGCT CAGATGCGGA 
     3181 TCCTAAAAGA GACGGAGCTA AGGAAGGTGA AGGTGCTTGG ATCAGGAGCT TTTGGCACTG 
     3241 TCTACAAGGG CATCTGGATC CCAGATGGGG AGAATGTGAA AATCCCCGTG GCTATCAAGG 
     3301 TGTTGAGAGA AAACACATCT CCTAAAGCCA ACAAAGAAAT TCTAGATGAA GCGTATGTGA 
     3361 TGGCTGGTGT GGGTTCTCCG TATGTGTCCC GCCTCCTGGG CATCTGCCTG ACATCCACAG 
     3421 TACAGCTGGT GACACAGCTT ATGCCCTACG GCTGCCTTCT GGACCATGTC CGAGAACACC 
     3481 GAGGTCGCCT AGGCTCCCAG GACCTGCTCA ACTGGTGTGT TCAGATTGCC AAGGGGATGA 
     3541 GCTACCTGGA GGACGTGCGG CTTGTACACA GGGACCTGGC TGCCCGGAAT GTGCTAGTCA 
     3601 AGAGTCCCAA CCACGTCAAG ATTACAGATT TCGGGCTGGC TCGGCTGCTG GACATTGATG 
     3661 AGACAGAGTA CCATGCAGAT GGGGGCAAGG TGCCCATCAA ATGGATGGCA TTGGAATCTA 
     3721 TTCTCAGACG CCGGTTCACC CATCAGAGTG ATGTGTGGAG CTATGGAGTG ACTGTGTGGG 
     3781 AGCTGATGAC TTTTGGGGCC AAACCTTACG ATGGAATCCC AGCCCGGGAG ATCCCTGATT 
     3841 TGCTGGAGAA GGGAGAACGC CTACCTCAGC CTCCAATCTG CACCATTGAT GTCTACATGA 
     3901 TTATGGTCAA ATGTTGGATG ATTGACTCTG AATGTCGCCC GAGATTCCGG GAGTTGGTGT 
     3961 CAGAATTTTC ACGTATGGCG AGGGACCCCC AGCGTTTTGT GGTCATCCAG AACGAGGACT 
     4021 TGGGCCCATC CAGCCCCATG GACAGTACCT TCTACCGTTC ACTGCTGGAA GATGATGACA 
     4081 TGGGTGACCT GGTAGACGCT GAAGAGTATC TGGTGCCCCA GCAGGGATTC TTCTCCCCGG 
     4141 ACCCTACCCC AGGCACTGGG AGCACAGCCC ATAGAAGGCA CCGCAGCTCG TCCACCAGGA 
     4201 GTGGAGGTGG TGAGCTGACA CTGGGCCTGG AGCCCTCGGA AGAAGGGCCC CCCAGATCTC 
     4261 CACTGGCTCC CTCGGAAGGG GCTGGCTCCG ATGTGTTTGA TGGTGACCTG GCAATGGGGG 
     4321 TAACCAAAGG GCTGCAGAGC CTCTCTCCAC ATGACCTCAG CCCTCTACAG CGGTACAGCG 
     4381 AGGACCCCAC ATTACCTCTG CCCCCCGAGA CTGATGGCTA TGTTGCTCCC CTGGCCTGCA 
     4441 GCCCCCAGCC CGAGTATGTG AACCAATCAG AGGTTCAGCC TCAGCCTCCT TTAACCCCAG 
     4501 AGGGTCCTCT GCCTCCTGTC CGGCCTGCTG GTGCTACTCT AGAAAGACCC AAGACTCTCT 
     4561 CTCCTGGGAA GAATGGGGTT GTCAAAGACG TTTTTGCCTT CGGGGGTGCT GTGGAGAACC 
     4621 CTGAATACTT AGTACCGAGA GAAGGCACTG CCTCTCCGCC CCACCCTTCT CCTGCCTTCA 
     4681 GCCCAGCCTT TGACAACCTC TATTACTGGG ACCAGAACTC ATCGGAGCAG GGGCCTCCAC 
     4741 CAAGTAACTT TGAAGGGACC CCCACTGCAG AGAACCCTGA GTACCTAGGC CTGGATGTAC 
     4801 CTGTAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4861 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4921 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4981 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     5041 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     5101 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5161 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5221 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5281 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5341 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5401 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5461 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5521 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5581 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5641 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5701 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5761 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5821 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5881 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5941 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     6001 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     6061 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6121 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6181 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6241 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6301 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6361 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6421 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6481 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6541 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6601 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6661 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6721 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6781 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6841 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6901 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6961 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     7021 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     7081 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7141 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7201 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7261 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7321 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7381 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7441 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7501 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7561 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7621 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7681 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7741 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7801 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7861 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7921 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7981 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8041 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     8101 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8161 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8221 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8281 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8341 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8401 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8461 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8521 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8581 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8641 AAATTTAACG CGAATTTT
//