LOCUS       RR205786                9069 bp    DNA     circular  SYN 
DEFINITION  Nid2 (Myc-DDK-tagged ORF) - Rat nidogen 2 (Nid2), (10 ug)
ACCESSION   NM_001012005
VERSION     NM_001012005.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5226..5317 
                     /label="Myc-DDK"  
     misc_feature    5347..5932 
                     /label="PolyA signal"  
     misc_feature    5404..5423 
                     /label="Restriction Enzyme"  
     misc_feature    6208..6880 
                     /label="ColE1"  
     misc_feature    7310..8104 
                     /label="Kan/Neo"  
     misc_feature    8139..8441 
                     /label="SV40 ori"  
     misc_feature    8527..9004 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5208..5214 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5208 
                     /label="Nid2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTTGGGAC CCGACAGCCG GGCGGCTGGC ACCGCCATCA CCGCTATCGC 
     1081 TGCTCCTGGT GCTGGTGCTG CTGTCACGAG TCGGGGCTCT GCGTCCTGAA GAGCTCTTTC 
     1141 CTTACGGGGA GTCGTGGGGA GACCGACTGC TGCCGGAGGG TGACGATGAA AGCTCAGCGG 
     1201 CTGTGAAGCT AGCAGTACCT CTACGCTTCT ACGATGCCCA GTTCAGCAAC CTCTACGTGG 
     1261 GCACCAACGG CATCATCTCC ACCCAGGACT TCCCCAGGGA GACTCAATAT GTAGATGATG 
     1321 ATTTTCCTAC TGACTTCCCA GCCATCGCCC CATTCCTGGC TGACATCGAC ACTAGTCACA 
     1381 GCAGGGGCCG GATCCTGTAC CGCGAGGACA CCTCCCAGGC TGTGCTGAGT CTGGCTGCCC 
     1441 GCTACGTGCG CACAGGCTTC CCGCTGACCG GGTCCAGCTT CACCCCTACC CACGCCTTCC 
     1501 TGGCCACCTG GGAGCACGTG GGCGCCTATG AGGAGGTCAG GCGCGGGGCT GCGCCATCCG 
     1561 GAGAGCTGAA CACTTTCCAG GCCGTTCTGG CATCCGATGA GTCTGATACC TACGCCCTCT 
     1621 TTCTCTATCC TGCCAATGGT CTTCAGTTCT TTGGAACTCG CCCCAAAGAG TCCTACAATG 
     1681 TCCAGCTGCA GCTTCCTGCC AGGGTGGGCT TCTGCCGAGG GGAAGCAGAC GACCTGAAGA 
     1741 GAGAAGCGCT GTATTTCAGC CTGACTAACA CTGAGCAGTC GGTGAAGAAT CTCTACCAAC 
     1801 TAAGCAACCT GGGGATTCCT GGAGCGTGGG CCTTTCACAT CGGCAGCAGG CTGGCCCTGG 
     1861 ACAATGTCCG GCCAGCCACA GTTGGAGGTG ACCATTCCAC AGCCCGCTCC TCAGCGCTGG 
     1921 AGCACTCCTT CAACCATGCT GCAGCCCTGG AGAGCTATAC TGAGGACAGT TTCGATTACT 
     1981 ACAATGAGAA TGAAGAGGAT GTAGAATACC CACCCATTGA ACCAGGGGAG GCCCTGGAAG 
     2041 GCCACAGCAG AATCGATGTG TCTTTCAATT CAGAGGTTAA TCCCACGTCT CCAGATTCTG 
     2101 ATCACGCTTC TCCTTTGCCA CACCCAGCAC CTGGTAACTG GCCATCCTAC CGGGAAACAG 
     2161 AATCGGCTTC TTTGGACCCT CAAACCAAAC AGGGGCCACC TGTGGGAGAG GTAGAGGTCC 
     2221 TGGATTTCAA GGACCCAGCA GAACTTTTGG ATCAGACGGG CACCAGAACC CCAGCTCCTC 
     2281 CAGGGGCAGA TGCAGCTTTC CTAACTCCAG GCAGGGAAGA CCTTGGGAAC AGAGACACCC 
     2341 AGTCCTATCC AGAGGCACGG CCAGTGCCTT CAGAGCCAGA TGTTCCTGTC GCTCCTCTGG 
     2401 AAAGAGAAAT CCTTCCTAAT TACCCCGAGT CTGGTCACGT GCCACCCCTG AGTGGAGGGA 
     2461 GGTATGTGGT AGGACTGGAG GACCACGTGA GTTCTAAAGA TCAAGTCTTC ACTTACAATG 
     2521 GCGCCAACCG GGAAACCTGT GAACACAGCC ACGGCCAGTG CTCCCGGCAT GCCTTCTGCA 
     2581 CCGACTACAC CACTGGCTTT TGCTGCCACT GCCAGTCTAG GTTTTATGGA AACGGGAGGC 
     2641 ACTGCGTGCC AGAAGGGGCG CCTCACCGAG TGAATGGGAA GGTGAGTGGC CATCTCCAAG 
     2701 TGGGCCACAT GCCTGTGCAC TTCACGGATG TGGATCTACA CGCTTACATC GTGAGCAATG 
     2761 ACGGCAGAGC CTACACTGCC ATCAGTCAGA TCCCGCAGCC CGCAGCCCAG GCCCTCCTCC 
     2821 CTGTCCTGCC CATCGGAGGC CTGTTTGGCT GGCTCTTTGC TTTGGAGAAG CCAGGCTCTG 
     2881 AGAATGGCTT CAGCCTCACA GGAGCTACCT TTGTCCATGA TGTGGAAGTC ACTTTTCACC 
     2941 CTGGAGAGGA GAGAGTTCGT ATCACTCAAA CCGCCGAGGG GCTTGACCCA GAGAACTATC 
     3001 TGAGCCTTAA CACCAACATC GAAGGCCAGG TGCCCTTTAT CCCAGCAAAT TTCACAGCCC 
     3061 ACATTGCTCC GTACCAAGAG TTCTATCATC ACAGAGACTC AGTCGTGACA TCCTCCAGCT 
     3121 CCCGAAGTTT CTCCCTCATT TCTGGTTCTA TCAACCAAAC TCGGTCCTAC CGCATCGACC 
     3181 AGAACATCAC TTACAAGGCG TGCAGTCATG CCCCCAGACA CCTGGCCGTC CCTGCCACCC 
     3241 AGCAGCTGAC TGTGGACCGG GCCTTTGCTT TGTACAGTGA GGAGGAGGGC GTGCTGAGGT 
     3301 TTGCCGTGAC CAATCAGATT GGCCCTGTCG AAGTGGACTC GGCCCCTACT GCAGTGAATC 
     3361 CTTGCTATGA CGGGAGCCAC ACCTGTGACA CAACAGCAAG GTGCCACCCG GGGACGGGTG 
     3421 TGGACTACAC CTGCGAGTGC ACGCCCGGGT TCCAGGGTGA CGGACGGAGC TGTGTGGATG 
     3481 TTAATGAGTG TGCCACTGGC TTCCATCGCT GCGGCCCCAA TTCTGTGTGT GTCAACTTGG 
     3541 TGGGGAGCTA CAGGTGCGAG TGCCGCAGCG GCTATGAATT CGCAGATGAC CGGCACACTT 
     3601 GTGTCTTGAT CGCCCCACCT CCCAACCCTT GCCTGGATGG CAGTCACACC TGTGCCCCTG 
     3661 AGGGGCAGGC CCGGTGCATT CACCATGGAG GCAGTTCGTT CAGCTGTGCC TGCCTGCCCG 
     3721 GCTTTGTTGG CACCGGGCAC CAGTGTTCTG ATGTTGACGA ATGTGCGGAA AACAGATGTC 
     3781 ATGGGGCAGC CATCTGTTAC AATACCCCCG GGTCCTTCTC CTGCCGGTGC CAGCCTGGGT 
     3841 ACCACGGGGA CGGGTTTCAC TGCGCCTCTG ACACAGTTCC AGAAGATTCC ATCTCAGGAC 
     3901 TGAAACCCTG TGAATACCAG CAGCGCTATG CCCAGGCACA GCATGCCCAC GCTGGGTCAC 
     3961 GGATCCACAT CCCCCAGTGC GACGACCAGG GAAACTTTGT GCCGCTGCAG TGCCATGGCA 
     4021 GCACTGGCTT CTGCTGGTGT GTGGACCAAA ATGGCCATGA AGTCCCTGGC ACCCAGACTC 
     4081 CACCTGGCTC CACTCCGCCC CACTGTGGAC CACCTCCAGA GCCCACCCAG AGGCCTCGGA 
     4141 CAGTCTGTGA GCGCTGGAGG GAAAGCCTGC TGGAACACTA CGGGGGCACA CCCAGGGACG 
     4201 ACCAGTATGT GCCCCAGTGT GACGACCTGG GCCACTTCAT CCCCCTGCAG TGTCACGGGA 
     4261 AGAGTGATTT CTGTTGGTGT GTGGACAAGG ATGGCAGAGA ACTTCCAGGC ACCCGCTCCC 
     4321 AGCCAGGCAC CATGCCTGCA TGCATACCCA CTGTCGCCCC ACCCGTGGTC CGGCCCACAC 
     4381 CCCGGCCTGA TGTGACTCCT CCGGCTGTGG GCACCTTCCT GCTCTATGCC CAGGGCCAGC 
     4441 AGATCGGTCA CTTGCCCCTC AATGGCAGCA GGCTTCAGAA GGATGCAGCC AGGACCCTGC 
     4501 TGTCACTACA TGGCTCCATA GTCGTGGGGA TTGACTATGA CTGCCGGGAG AGGATGGTCT 
     4561 ACTGGACAGA CGTTGCCGGG AGGACCATCA GCCGTGCCAG CTTGGAGGCA GGAGCCGAAC 
     4621 CCGAGACCAT CATTACCTCA GGTCTGATAA GCCCAGAAGG ACTTGCCATC GACCACTTCC 
     4681 GGAGAACGAT GTACTGGACA GACAGCGGCC TGGATAAGAT AGAGCGGGCT GGACTGGACG 
     4741 GTTCCGAGCG TAAGGTCCTC TTCCACACAG ATCTGGTGAA TCCACGAGCT ATCACTGTGG 
     4801 ATCCAATCCG AGGCAACTTG TACTGGACAG ACTGGAATAG AGAAGCTCCT AAAATTGAAA 
     4861 CATCATCTTT AGATGGTGAA AACAGAAGAA TTCTGATCAA CAAAGATATT GGATTACCCA 
     4921 ATGGATTGAC CTTTGACCCC TTCTCCAAAC TTCTCTGCTG GGCAGATGCA GGAACCAAAA 
     4981 AACTGGAGTG TACACTACCT GATGGAACTG GACGCCGCGT CATCCAAAAC CACCTCAATT 
     5041 ACCCCTTCAG TGTTGTCAGT TACGCGGATC ACTTCTACCA CACGGACTGG CGGAGGGATG 
     5101 GTGTTATATC AGTGAGTAAA GACAGTGGCC AGCTTACTGA TGAGTATCTT CCTGAACAGC 
     5161 GCTCTCACCT CTATGGCATC ACTGCAGTCT ATCCCTACTG TCCAACAGGA AGAAAGACGC 
     5221 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5281 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5341 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5401 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5461 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5521 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5581 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5641 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5701 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5761 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5821 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5881 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5941 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     6001 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     6061 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     6121 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6181 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6241 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6301 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6361 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6421 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6481 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6541 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6601 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6661 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6721 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6781 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6841 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6901 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6961 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     7021 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     7081 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     7141 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7201 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7261 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7321 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7381 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7441 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7501 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7561 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7621 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7681 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7741 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7801 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7861 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7921 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7981 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     8041 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     8101 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     8161 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8221 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8281 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8341 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8401 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8461 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8521 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8581 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8641 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8701 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8761 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8821 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8881 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8941 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     9001 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     9061 GCGAATTTT
//