LOCUS       RR205764                8748 bp    DNA     circular  SYN 
DEFINITION  Ptprj (Myc-DDK-tagged ORF) - Rat protein tyrosine phosphatase, receptor type, J (Ptprj), (10 ug)
ACCESSION   NM_017269
VERSION     NM_017269.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4905..4996 
                     /label="Myc-DDK"  
     misc_feature    5026..5611 
                     /label="PolyA signal"  
     misc_feature    5083..5102 
                     /label="Restriction Enzyme"  
     misc_feature    5887..6559 
                     /label="ColE1"  
     misc_feature    6989..7783 
                     /label="Kan/Neo"  
     misc_feature    7818..8120 
                     /label="SV40 ori"  
     misc_feature    8206..8683 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4887..4893 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4887 
                     /label="Ptprj"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCCCGGGG AAGCCCGGGG CGGGCGGAGC GGGGACTAGG CGGACCGGCT 
     1081 GGCGGAGAAG GAGGCGGAGG CGTCGGCTGG AGGCGGAGAC CAGGGCGCCC GGCTTCGGGC 
     1141 ACACGGCGGG GCGCGTCCCG GGCACGTTCC AGGGCGCGCA GGGCATGAAG CCCGCGGCGC 
     1201 GGGAGACGCG GACACCCCCG CGCTCGCCCG GGCTCCGCTG GGCGCTGCTG CCGCTGCTGC 
     1261 TGTTGCTACG CCAGGGCCAG GTTGTGTGCG CAGGTGCGGC GCCCAGTCCT GTATTTGACG 
     1321 TTGAAGCTGT CACCAGCCCT ACCAGTGTGG TATTAACGTG GAAACACAAT GATTCAGCCA 
     1381 CTTCAGAATA TAAGATAAAT GAGGGAAACA CGCTGCGGTA TACTGTTAAA AACCAGACAT 
     1441 CATTTAACAT CACAGGCTTA AGCCCAGCTA CTTCCTACAA ATTCTCCATC ACCTTGGGAA 
     1501 CAGTCAATGA GACCTCAGGA AAGCCCACAT ACAAAAATAT CACAACAGAG CCCTGGCCAG 
     1561 TGTCGGATCT CCAAGTCGCC TACATCGGTG TGACACAGGC TCTTCTCGCC TGGAGCAATG 
     1621 CAAACGGCAC CGCTTCTTAC CGGATGCAGA TTGTAGAGCT GACCACAAAT TCATCAGGTG 
     1681 GTATTTCAGA TCTGAAGCCG GGGACCCATA AGAGCTTGGC TGTCCAAGGA TCGAATGAGA 
     1741 CCCAGCATGA CTTATGGGTC ACAGAAGGCG TCTCGGATGC CAATGGCACC GAGGGAAGCC 
     1801 CAGTGGCCAA CATATCCCAA CTACATAAGA ATTCTCTTGC CTCTGCAGAC CCACCCTCTG 
     1861 CCCGGGATCC CTCCCTCACA GAGGTCTTGC TCACTGAGCT AAAGCCTGAT ACTCAGTACA 
     1921 AAGTCACCAT TTATTCTCAA GCAGCAGATG GCACAGAAGG ACAGCCTGGG AACAAAGTGT 
     1981 TTAAAACAAA TCCCATCCAA GTTTCTGACA TCCGAGCTGT GAACATCAGT GACTCGAACA 
     2041 TGACCCTGAC CTGGAAAAGC AACAATAATG AGTCCCATGC TTCATTTACC TACAAAATAT 
     2101 ACGTGGCTGG GGGTTCCGAC TCCATCAACG AAACTGTCAA TGAGACTCAG GCCGTCATCC 
     2161 GTGGACTCAG CTCCAGTACC TTGTACAACA TCACAGTGCT TCCTTTCCTG GGTCAGACCG 
     2221 CGGGTATACC AGGCTTCCTC CAAGTGTACA CTTCCCCCCG TCCTGTTTCT GACTTCCGAG 
     2281 TGACAAATGT CAGCCTGAGG GAAATCGGTT TGGCTTGGAG AAGCAATGAC TCGGAGTCAT 
     2341 TCGAGATTTT CATCACGCAG GAGGGAAGTG AGAAACGTTG GAATGCTTCG ACGGGAGACC 
     2401 TGAGCTATAT CGTTGAAAAT TTAAAGCCTG GGACCAGTTA CCAATTTGAA ATATTCCCAC 
     2461 GAGGACCAAA TGGGACTGAA GGGCCATCCC AGACAGTTGT TGGTAGAACT GACTGCAGTG 
     2521 CTGTGACTGA CATCCGCGTG GTCAGCGTTA GCACCACTGA AATACAGCTG GAGTGGCAGA 
     2581 ATACAGACAG TGCTTCTGGA TACACTTACC ATTTAGTTCT AGAGTCTGAG AATGGCTCCA 
     2641 TCAAGACCAA CAGTTCTCAG AAATGGATCA CAGTGGGGGG CCTTACCCCA GGCACCTTAT 
     2701 ACAATGTTAC AATCTTTCCA GAAGTGGACC AGATGGAGGG CAACTCCAGC TCTATTACCC 
     2761 AGTACACAAG GCCCAGCAAT GTGTCCTACA TTGAAGTAAA CACCAACACC ACCGTGGCAG 
     2821 CCATCCAGTG GAAGAACCTG GACGCAGCCT CTGCTTCCTA CTCCTACTCC GTTCTTATCT 
     2881 TGAAGGCTGG AGATGGCAGC AATGTAACCA GCAGGGTCAG AGACATCCCT TCTGTCACCA 
     2941 TCCCTGGGCT AATCCCCGGC GTCTCTTACG AAGTGAAGAT CTTTACAAAG ATTAGGAATA 
     3001 CAGAGGTTGG GAATGAGGTA CCTGGTCAGA AGCTGTTCTG TATGGAACCT GCACAAGTGG 
     3061 ATTCCTTACA CTGTGAAGTG GTCCCGAAGG AGCCAGCGCT GGTTCTGAAG TGGGCCTGCC 
     3121 CCCCTGGCAT GAACTCAGGC TTTGAACTGG GGGTCAGGAG TGATGCTTGG GACAATATGA 
     3181 CACACTTGGA GAACTGCACT TTGGACAATG ACACAGAGTG CAGGACAGAA GTCACCTATT 
     3241 TGAATTTTTC TACCTCGTAC AACATCAGCA TTGCCACCTT GTCATGTGGA AAGATGGCAT 
     3301 TGCCCACCCA GAGCACCTGC ACCACTGGCA TCACAGACCC ACCTCCTCCA GATGGATCCC 
     3361 CTAATATTAC ATCGGTCAGT CACAATTCAG TAAAGGTGAA GTTCAGTGGT TTTGAAGCCA 
     3421 GCCATGGACC TATCAAAGCC TATGCTGTCC TTCTCACCAC AGGGGAAGCT GGCCAACCCT 
     3481 CCACAGATGT TTTGAAGTAC ACATATGAAG ATTTCAAAAA GGGAGCCTCC GATACTTATG 
     3541 TGACATACCT CATAAGGATA GAAGAGAAGG GACAGTCTCA GGGCTTGTCT GAAGCATTGA 
     3601 ACTATGAGAT TGATGTGGGG AACCAATCCA CTACCCTCGG CTACTACAAC GGGAGGCTAG 
     3661 AGCCTCTGGG CTCCTACCGG GCTTGCGTTG CTGGCTTTAC CAATATTACC TACAACCTTC 
     3721 AGAACGATGG CCTTATTAAT GGGGATGAGA GCTATGTATC TTTCAGTCCG TATTCAGAGG 
     3781 CCGTGTCCTT GCCTCAAGAT CCAGGTGTCA TCTGCGGAGC GGTGTTCGGA TGTATCTTTG 
     3841 GTGCCCTGGC CATTGTGGCT GTGGGAGGCT TCATCTTCTG GAGAAAGAAA AGGAAAGATG 
     3901 CCAAGAATAA TGAAGTGTCC TTTTCTCAAA TTAAATCCAA GTTAATCCGA GTGGAGAATT 
     3961 TTGAGGCCTA CTTTAAGAAA CAGCAAGCCG ACTCCAACTG TGGGTTTGCA GAGGAATATG 
     4021 AGGATCTGAA GCTGATTGGA ATAAGTTTAC CTAAATATGC AGCGGAAATA GCTGAAAACA 
     4081 GGGGGAAGAA CCGCTACAAC AACGTCCTGC CCTATGATAT TTCTCGAGTC AAACTTTCAG 
     4141 TCCAGACCCA TTCGACAGAC GACTACATCA ATGCCAACTA TATGCCTGGC TACCATTCCA 
     4201 AGAAAGATTT CATTGCCACA CAAGGACCTT TACCCAACAC TTTGAAAGAT TTTTGGCGTA 
     4261 TGGTTTGGGA GAAAAACGTA TATGCCATTG TTATGCTGAC CAAATGTGTG GAGCAGGGAA 
     4321 GGACCAAATG TGAAGAGTAC TGGCCTTCCA AGCAGGCTCA GGACTACGGG GACATAACTG 
     4381 TGGCAATGAC ATCAGAAGTT GTTCTTCCGG AATGGACCAT CAGAGATTTT GTGGTGAAAA 
     4441 ATATGCAGAG TAGTGAGAGT CATCCTCTGC GGCAGTTCCA TTTCACCTCC TGGCCTGACC 
     4501 ATGGTGTTCC TGACACCACC GACCTGCTCA TCAACTTTCG GTACCTGGTC CGGGATTACA 
     4561 TGAAGCAGAT CCCCCCTGAG TCACCAATCC TGGTGCATTG CAGTGCTGGG GTTGGAAGGA 
     4621 CGGGCACTTT CATTGCTATT GATCGCCTGA TCTATCAGAT AGAGAACGAG AACACCGTGG 
     4681 ATGTGTATGG CATTGTCTAC GATCTTCGGA TGCACAGGCC TCTGATGGTA CAGACAGAGG 
     4741 ACCAGTATGT TTTCCTCAAT CAGTGTGTGT TGGATATTAT CAGAGCCCAG AAAGACTCAA 
     4801 AAGTTGACCT CATCTATCAG AACACAACCG CAATGACAAT CTATGAAAAC CTCGAGCCAG 
     4861 TGAGCATGTT TGGAAAGACT AATGGTTACA TCGCCACGCG TACGCGGCCG CTCGAGCAGA 
     4921 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4981 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     5041 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     5101 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5161 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5221 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5281 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5341 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5401 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5461 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5521 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5581 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5641 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5701 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5761 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5821 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5881 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5941 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     6001 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     6061 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6121 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6181 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6241 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6301 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6361 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6421 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6481 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6541 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6601 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6661 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6721 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6781 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6841 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6901 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6961 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     7021 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     7081 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7141 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7201 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7261 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7321 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7381 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7441 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7501 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7561 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7621 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7681 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7741 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7801 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7861 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7921 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7981 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8041 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     8101 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8161 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8221 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8281 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8341 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8401 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8461 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8521 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8581 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8641 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8701 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//