LOCUS       RR205715                8988 bp    DNA     circular  SYN 
DEFINITION  Rock1 (Myc-DDK-tagged ORF) - Rat Rho-associated coiled-coil containing protein kinase 1 (Rock1), (10 ug)
ACCESSION   NM_031098
VERSION     NM_031098.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5145..5236 
                     /label="Myc-DDK"  
     misc_feature    5266..5851 
                     /label="PolyA signal"  
     misc_feature    5323..5342 
                     /label="Restriction Enzyme"  
     misc_feature    6127..6799 
                     /label="ColE1"  
     misc_feature    7229..8023 
                     /label="Kan/Neo"  
     misc_feature    8058..8360 
                     /label="SV40 ori"  
     misc_feature    8446..8923 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5127..5133 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5127 
                     /label="Rock1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGACTGGG GACAGTTTTG AGACTCGGTT TGAAAAAATC GACAACCTGC 
     1081 TGCGGGATCC CAAATCGGAA GTGAATTCAG ATTGTTTGCT GGATGGATTG GATGCTTTGG 
     1141 TTTATGATTT GGATTTTCCT GCCTTAAGAA AAAACAAAAA TATTGACAAC TTTCTAAGCA 
     1201 GATATAAAGA CACAATAAAT AAAATTAGAG ATTTACGGAT GAAAGCTGAA GATTATGAAG 
     1261 TAGTAAAGGT AATCGGCAGA GGTGCATTTG GAGAAGTTCA ATTGGTAAGG CATAAATCCA 
     1321 CCAGGAAGGT TTATGCTATG AAGCTTCTCA GCAAATTTGA AATGATTAAG AGATCTGATT 
     1381 CTGCTTTTTT CTGGGAAGAA AGGGACATCA TGGCATTTGC CAATAGTCCT TGGGTTGTTC 
     1441 AGCTTTTTTA TGCATTCCAA GATGACCGAT ATCTCTATAT GGTGATGGAG TACATGCCTG 
     1501 GTGGAGACCT TGTAAACCTG ATGAGCAACT ACGATGTGCC TGAAAAGTGG GCACGATTCT 
     1561 ATACTGCAGA AGTAGTTCTC GCATTGGATG CRATCCATTC CATGGGTTTT ATTCATAGAG 
     1621 ATGTGAAGCC TGACAACATG CTGCTGGATA AGTCTGGACA TTTGAAGTTA GCCGACTTTG 
     1681 GTACTTGTAT GAAGATGAAT AAGGAAGGTA TGGTACGATG TGATACAGCA GTTGGAACAC 
     1741 CTGATTATAT TTCACCTGAA GTATTAAAAT CACAAGGCGG TGATGGCTAT TATGGACGAG 
     1801 AGTGTGACTG GTGGTCAGTT GGTGTATTTT TATATGAAAT GCTTGTAGGT GATACACCTT 
     1861 TTTATGCAGA TTCTTTGGTT GGGACGTACA GTAAAATTAT GAACCATAAA AATTCACTTA 
     1921 CTTTCCCTGA TGATAATGAC ATATCAAAAG AGGCGAAAAA TCTCATTTGT GCCTTCCTTA 
     1981 CGGACAGAGA AGTGAGATTG GGCAGAAATG GTGTAGAGGA AATCAAACGT CATCTCTTCT 
     2041 TCAAAAATGA TCAGTGGGCT TGGGAAACGC TCCGAGACAC TGTAGCACCA GTTGTGCCTG 
     2101 ATTTAAGTAG TGACATTGAT ACTAGTAATT TTGATGACTT AGAAGAAGAT AAAGGAGATG 
     2161 AAGAAACATT TCCTATTCCA AAAGCTTTTG TTGGCAATCA GCTACCTTTT GTAGGATTTA 
     2221 CATATTATAG CAATCGTAGA TACTTACCTT CAGCAAATCC CAGTGAGAAC AGATCTAGCT 
     2281 CCAATGTGGA TAAAAACGTG CAGGAAAGTT TACAGAAAAC AATCTACAAG CTTGAAGAAC 
     2341 AACTGCATAA TGAAATGCAA TTAAAAGATG AAATGGAGCA AAAGTGCAGA ACTTCAAACA 
     2401 TAAAACTAGA CAAGATAATG AAAGAATTGG ATGAAGAGGG GAATCAAAGA AGAAATCTAG 
     2461 AATCTGCGGT GTCTCAAATT GAGAAAGAAA AGATGTTGCT ACAACATAGA ATTAATGAGT 
     2521 ACCAAAGAAA AGTGGAACAG GAAAATGAGA AAAGAAGAAA TGTAGAAAAT GAAGTTTCTA 
     2581 CACTAAAGGA CCAGTTGGAA GACTTACGGA AAGCAAGTCA GAGCTCACAG CTTGCTAATG 
     2641 AGAAGCTGAC ACAGTTACAG AAGCAGTTAG AAGAAGCGAA TGACTTACTT AGGACAGAAT 
     2701 CAGACACAGC TGTAAGATTA AGGAAGAGTC ACACAGAGAT GAGCAAGTCA GTTAGTCAGC 
     2761 TGGAGTCTCT GAACAGAGAG TTACAGGAGA GAAACAGAAT GTTAGAGAAT TCCAAGTCAC 
     2821 AAGCAGACAA GGATTACTAC CAGCTGCAAG CTGTATTGGA AGCTGAGCGA AGAGACAGAG 
     2881 GCCATGATTC TGAGATGATT GGAGACCTTC AAGCACGAAT TACGTCTTTA CAAGAAGAGG 
     2941 TGAAGCATCT CAAACATAAT CTTGAAAGAG TAGAAGGAGA AAGAAAAGAG GCTCAAGACA 
     3001 TGCTCAATCA TTCAGAAAAG GAAAAAAATA ATTTGGAGAT TGATTTGAAC TACAAGCTCA 
     3061 AGTCTATTCA GCAGAGGTTA GAACAAGAAG TAAATGAACA CAAAGTAACC AAAGCGCGGC 
     3121 TAACTGACAA GCATCAGTCT ATTGAAGAGG CAAAGTCTGT TGCCATGTGT GAAATGGAAA 
     3181 AAAAGCTGAA GGAAGAGAGA GAAGCTCGAG AGAAGGCTGA AAATAGAGTT GTTGAGACTG 
     3241 AGAAGCAATG TTCTATGCTA GATGTTGACC TGAAACAGTC TCAGCAGAAG CTAGAACATT 
     3301 TGACCGAAAA TAAAGAAAGG CTGGAGGATG CAGTTAAGAG TCTCACCCTG CAGCTGGAAC 
     3361 AGGAATCCAA TAAGCGGATC TTGCTACAGA GTGAACTGAA GACTCAAGCG TTTGAAGCAG 
     3421 ACAATTTAAA AGGGTTAGAA AAACAGATGA AACAGGAAAT AAATACTTTA TTGGAAGCTA 
     3481 AAAGATTGTT AGAATTTGAG TTAGCTCAGC TTACAAAGCA ATACAGAGGA AATGAAGGGC 
     3541 AAATGCGGGA GTTACAAGAT CAGCTTGAAG CTGAGCAATA TTTCTCTACA CTTTATAAAA 
     3601 CCCAGGTAAA GGAACTTAAA GAAGAAATTG AAGAAAAAAA CAGAGAAAAT TTAAGGAAAA 
     3661 TACAGGAGCT GCAAAGTGAA AAAGAAACTC TTTCGACTCA GTTGGATTTA GCAGAAACAA 
     3721 AAGCTGAGTC TGAGCAGTTG GCTCGAGGAA TTCTGGAAGA ACAGTATTTT GAGTTGACTC 
     3781 AAGAAAGCAA GAAAGCTGCT TCAAGAAATA GACAAGAGAT TACAGATAAA GATCATACCG 
     3841 TTAGTCGGCT TGAAGAAGCA AACAATGCGC TAACCAAAGA TATTGAACTA CTAAGAAAAG 
     3901 AAAATGAAGA GCTAAATGAA AGGATGAGAA CAGCAGAAGA AGAATATAAA TTGAAGAAGG 
     3961 AGGAAGAAAT AAGTAATCTT AAGGCTGCCT TTGAAAAGAA TATCAGTACC GAACGGACCC 
     4021 TTAAAACACA GGCTGTTAAC AAGTTGGCAG AAATAATGAA TCGCAAAGAT TTTAAAATTG 
     4081 ATAGGAAGAA AGCTAATACT CAAGATTTGA GAAAAAAAGA AAAGGAAAAT AGAAAGCTCC 
     4141 AACTAGAACT TAACCAAGAA AGAGAAAAAT TTAACCAAAT GGTGGTGAAA CACCAGAAGG 
     4201 AGCTGAATGA CATGCAAGCG CAATTGGTAG AAGAATGTAC ACATAGAAAT GAACTTCAAA 
     4261 TGCAGTTGGC TAGCAAAGAG AGTGATATTG AGCAGTTGCG TGCAAAACTT TTGGACCTTT 
     4321 CGGATTCAAC TAGTGTTGCT AGTTTTCCCA GTGCTGATGA GACTGACGGA AATCTTCCAG 
     4381 TTGGTTCTGC CTGCATTCCT TATTTATTTA TCTTCTACTC TTCTAGTTCA AGAATTGAAG 
     4441 GTTGGCTTTC TGTACCAAAT AGAGGAAATA TCAAAAGATA TGGCTGGAAG AAACAGTATG 
     4501 TTGTGGTGAG CAGCAAAAAA ATGTTGTTCT ATAATGATGA GCAAGATAAG GAACAATCCA 
     4561 GTCCATCTAT GGTGTTGGAC ATAGATAAAT TGTTCCATGT CCGACCTGTA ACCCAAGGAG 
     4621 ATGTGTACAG AGCTGAAACT GAAGAAATTC CTAAAATATT CCAGATACTG TATGCAAATG 
     4681 AAGGTGAATG TAGAAAAGAT ATAGAAGTGG AACCAGTTCA ACAAGGTGAA AAAACTAATT 
     4741 TCCAGAATCA CAAAGGCCAT GAATTCATTC CTACCCTCTA CCACTTTCCA GCCAACTGCG 
     4801 AGGCCTGTGC CAAACCTTTG TGGCATGTTT TTAAGCCACC GCCTGCCCTA GAGTGTCGAA 
     4861 GATGCCATGT TAAGTCCCAC AGGGACCATC TAGACAAGAA AGAGGACTTG ATTCCCCCAT 
     4921 GCAAAGTAAG TTATGATGTA ACATCAGCAA GAGATATGTT GCTGTTAGCA TGTCCTCAGG 
     4981 ATGAACAAAA GAAATGGGTA ACTCATCTGG TAAAGAAGAT TCCTAAGAAG GCACCTTCTG 
     5041 GCTTTGTCCG TGCCTCTCCT CGAACGCTGT CGACAAGATC TACTGCAAAT CAGTCTTTCC 
     5101 GGAAAGTAGT CAAAAATACA TCTGGAAAAA CTAGTACGCG TACGCGGCCG CTCGAGCAGA 
     5161 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     5221 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     5281 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     5341 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5401 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5461 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5521 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5581 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5641 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5701 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5761 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5821 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5881 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5941 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     6001 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     6061 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     6121 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     6181 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     6241 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     6301 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6361 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6421 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6481 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6541 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6601 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6661 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6721 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6781 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6841 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6901 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6961 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     7021 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     7081 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     7141 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     7201 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     7261 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     7321 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7381 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7441 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7501 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7561 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7621 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7681 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7741 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7801 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7861 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7921 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7981 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     8041 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     8101 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     8161 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     8221 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8281 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     8341 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8401 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8461 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8521 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8581 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8641 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8701 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8761 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8821 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8881 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8941 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//