LOCUS       RR205699                8289 bp    DNA     circular  SYN 
DEFINITION  Srebf2 (Myc-DDK-tagged ORF) - Rat sterol regulatory element binding transcription factor 2 (Srebf2), (10 ug)
ACCESSION   NM_001033694
VERSION     NM_001033694.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4444..4535 
                     /label="Myc-DDK"  
     misc_feature    4565..5150 
                     /label="PolyA signal"  
     misc_feature    4622..4641 
                     /label="Restriction Enzyme"  
     misc_feature    5426..6098 
                     /label="ColE1"  
     misc_feature    6528..7322 
                     /label="Kan/Neo"  
     misc_feature    7357..7659 
                     /label="SV40 ori"  
     misc_feature    7745..8222 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4419..4426 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4419 
                     /label="Srebf2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGAGAAC AGCGAGCTGG GCGGTCTGGA GACCATGGAG ACCCTCACGG 
     1081 AGCTGGGCGA TGAGCTGACT CTCGGGGACA TCGACGAGAT GCTACAGTTT GTCAGCAATC 
     1141 AAGTGGGAGA GTTCTCTGAC TTGTTTTCAG AGCAACTATG TAGCTCCTTC CCTGGAGGTG 
     1201 GTGGCGGCAG TGGCAGTGGT GGCACCAGCA ACAACAGCAG TGGCAGAGGC ACCAGTGGTG 
     1261 GAGCTGCAGA TCCCGCAGTA CAGCGGTCAT TCAGCCAGGT CCCATTGTCT ACCTTCTCTC 
     1321 CCTCATCCAC CTCCCCTCAG GCTCCAGCCC TGCAGGTCAA AGTCTCTCCT ACCCCACCAA 
     1381 GGGCAACCCC TGTTCTCCAG CCCCGACCCC AGCCCCAGCC TCAACCTCCA GCTCAGCTGC 
     1441 AGCAGCAGAC GGTGATGATC ACCCCGACCT TCAGCACCGC TCCACAGACC AGGATCATCC 
     1501 AGCAGCCTTT GATATACCAG AATGCCGCAA CCAGCTTTCA AGTCCTGCAG CCTCAAGTGC 
     1561 AAAGCCTCGT GACATCCTCG CAGGTACAGC CAGTTACCAT CCAGCAGCAG GTGCAGACGG 
     1621 TACAGGCCCA GCGGGTGTTG ACTCAGACAG CCAATGGCAC ACTGCAGACC CTTGCCCCAG 
     1681 CCACAGTGCA GACAGTCGCT ACACCACAGG TGCAGCAGGT CCCGGTCCTG GTTCAGCCTC 
     1741 AGATTATTAA GACAGATTCC CTTGTTTTGA CCACGCTGAA AACAGATGGC AGCCCTGTCA 
     1801 TGGCCGCCGT CCAGAACCCG GCCCTCACTG CCCTCACCGC CCCCATCCAG ACGGCCGCCC 
     1861 TTCAAGTACC AACCCTGGTG GGCAGCAACG GGGCCATTCT GACCACAATG CCGGTAATGA 
     1921 TGGGCCAAGA GAAAGTCCCC ATTAAGCAAG TGCCTGGGGG TGTAAAGCAG CTCGAGCCTC 
     1981 CCAAAGAAGG AGAGAGGCGG ACAACACACA ATATCATTGA GAAGCGGTAC CGGTCCTCCA 
     2041 TCAACGACAA AATCATAGAG TTGAAAGACT TGGTCATGGG GACAGATGCC AAGATGCACA 
     2101 AGTCTGGCGT TCTGAGGAAG GCCATTGATT ACATCAAGTA CCTGCAGCAG GTCAACCACA 
     2161 AGCTGCGCCA GGAGAACATG GTGCTGAAGC TGGCAAATCA GAAAAACAAG CTCCTGAAGG 
     2221 GCATCGACTT GGGCAGTCTG GTGGACAGTG ATGTGGACTT GAAGATCGAT GACTTCAATC 
     2281 AGAATGTCCT TCTGATGTCT CCCCCGGCCT CGGACTCCGG GTCCCAGGCT GGCTTCTCCC 
     2341 CCTATTCCAT TGACTCTGAG CCGGGCAGCC CTCTGCTGGA TGATGCAAAG GTCAAGGATG 
     2401 AACCGGACTC TCCCCCTGTG GCACTGGGCA TGGTGGACCG ATCTCGAATT CTCTTATGTG 
     2461 TCCTCACCTT CCTGGGTCTC TCCTTTAACC CCTTGACTTC ATTGCTGCAG TGGGGAGGGG 
     2521 CCCACAACCC TGACCAACAC CCGTACTCAG GCTCTGGCCG CAATGTACTG TCACTGGAGT 
     2581 CAGGTTCTGG AGGCTGGTTT GACTGGATGA TGCCGACTCT ACTGCTGTGG CTGCTAAACG 
     2641 GTGTGATTGT CTTGAGCGTC TTCGTGAAGC TGTTGGTCCA TGGGGAGCCA GTGATCCGCC 
     2701 CACACTCACG CTCCTCGGTC ACCTTCTGGA GACACCGGAA GCAGGCAGAC CTAGACCTCG 
     2761 CCAAAGGTGA CTTTGCAGCT GCTGCTGCCA ACCTACAGAC CTGCTTATCC GTTTTGGGCC 
     2821 GGGCGCTGCC CACCTCCCGC CTGGACTTGG CCTGCAGCCT CTCCTGGAAC GTGATCCGCT 
     2881 ACAGCCTGCA GAAGCTGCGC CTGGTACGCT GGTTACTCAA AAAGGTCTTC CAGCGCTGGC 
     2941 GGGCTACCCC CGCCACTGCA GCCGGCTTTG AGGATGAAGC TAAGAGCAGT GCGAGGGATG 
     3001 CGGCCCTGGC CTACCACAGG CTGCACCAGC TGCACATCAC AGGGAAGCTT CCAGCAGGAT 
     3061 CTGCCTGTTC CGATGTGCAC ATGGCTCTGT GTGCTGTGAA CCTCGCCGAG TGTGCAGAGG 
     3121 AGAAAATCCC ACCAAGCACA CTTGTCGAGA TCCACCTGAC CGCTGCCATG GGGCTCAAGA 
     3181 CCCGATGCGG GGGCAAGCTG GGCTTCTTGG CTAGCTACTT CTTAAATCGT GCCCAGAGTC 
     3241 TGTGTGGCCC AGAGCATAGC GCTGTCCCCG ACTCCCTGCG CTGGCTGTGC CATCCCCTGG 
     3301 GCCAGAAGTT TTTCATGGAG CGAAGCTGGT CGATCAAGTC AGCAGCCAAG GACAGCCTGT 
     3361 ACTGCGCCCA GAGGAACCCA GCTGACCCCA TTGCCCAGGT ACACCAGGCT TTCTGCAAAC 
     3421 ACCTGCTGGA AAGAGCTGTG GAGGCCTTGG TGAAGCCTCA GGCCAAGAAG AAGGCAGGTG 
     3481 ACCGGGAAGA AGAGAGCTGT GAATTTTCCA GTGCTCTAGA GTTTTTGAAA TTACTTCACT 
     3541 CTTTTGTGGA CTCTGTGGGA TTTGTGGCCT CACCATTCTC CAGCAGTTCT GTGCTCAGGT 
     3601 CAGCCCTTGG TCCCGATGTC GTCTGTCGGT GGTGGACGTC CGCGATCACC GTGGCCATCA 
     3661 GCTGGCTCCA GGGCGATGAT GCAGCTGTGC GCTCGCATTT CACTGAAGTA GAGCGGGTCC 
     3721 CCAAGGCCCT GGAAGTGACT GAGAGTCCCT TGGTGAAGGC CGTCTTCTAT GCCTGTAGAG 
     3781 CCATGCATGC CTCACTCTCT GGAAAGGCCG ATGGGCAACA GAACTCCTTC TGTCATTGTG 
     3841 AGAGGGCCAG TGGTCACCTG TGGAGCAGTC TCAATGTCAG CGGCACTACT TCTGACCCTT 
     3901 CACTTAACCA TGTGGTCCAG CTGCTCACCT GTGATCTGCT ACTGTCACTG AGGACAACAC 
     3961 TCTGGCAAAA GCAGGCCAGC GCCAGCCAGC TCCTGGGCGA GACCTACCAC GCGTCAGGCA 
     4021 CTGAGCTGGC CGGCTTCCAG CGGGACCTGG GCAGCCTTCG AAGGCTGGCC CACAGTTTCC 
     4081 GCCCAGCATA CCGCAAGGTG TTCCTGCATG AAGCAACTGT TCGACTAATG GCGGGAGCCA 
     4141 GCCCCACCCG TACACACCAG CTGCTGGAGC ACAGCCTACG GCGCCGCACC ACTCAGAACA 
     4201 CCAAACACGG AGAAGTAGAC ACCTGGCCTG GCCAGCGAGA ACGAGCCACT GCCATCCTGC 
     4261 TGGCCTGCCG CCATCTTCCC CTCTCCTTCC TATCTTCCCC GGGCCAGCGG GCAGTGCTGC 
     4321 TGGCTGAAGC TGCCCGCACC CTGGAGAAGG TGGGTGACCG CCGTTCCTGC AGTGACTGCC 
     4381 AGCAGATGAT TGTCAAGCTG GGCGGTGGCA CTGCCATCGC TGCTTCCAGC GGACCGACGC 
     4441 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4501 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4561 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4621 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4681 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4741 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4801 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4861 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4921 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4981 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5041 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5101 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5161 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5221 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5281 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5341 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5401 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5461 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5521 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5581 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5641 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5701 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5761 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5821 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5881 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5941 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6001 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6061 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6121 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6181 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6241 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6301 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6361 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6421 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6481 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6541 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6601 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6661 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6721 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6781 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6841 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6901 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6961 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7021 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7081 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7141 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7201 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7261 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7321 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7381 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7441 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7501 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7561 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7621 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7681 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7741 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7801 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7861 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7921 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7981 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8041 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8101 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8161 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8221 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8281 GCGAATTTT
//