LOCUS       RR205675                7284 bp    DNA     circular  SYN 
DEFINITION  Tfr2 (Myc-DDK-tagged ORF) - Rat transferrin receptor 2 (Tfr2), (10 ug)
ACCESSION   NM_001105916
VERSION     NM_001105916.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3439..3530 
                     /label="Myc-DDK"  
     misc_feature    3560..4145 
                     /label="PolyA signal"  
     misc_feature    3617..3636 
                     /label="Restriction Enzyme"  
     misc_feature    4421..5093 
                     /label="ColE1"  
     misc_feature    5523..6317 
                     /label="Kan/Neo"  
     misc_feature    6352..6654 
                     /label="SV40 ori"  
     misc_feature    6740..7217 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3414..3421 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3414 
                     /label="Tfr2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAACAACGT TGGGGTCTAC TTCGGAAAGT GCAACAGTGG TCCCCAAGAC 
     1081 CCTCTCAGAC CATCTACAGA CGTGTGGAAG GCCCTCAACT GGAGAACCTA GAGGAGGAAG 
     1141 ATAGGGAGGA AGGGGAGGAG CTTCCTGCCC AGTTCTGCCC CATGGAACTC AAAGGCCCTG 
     1201 AGCGCTTAGG CTCCTGTCCT GGGAGGTCCA TTCCCATACC CTGGGCTGCA GCAGGTCGAA 
     1261 AGGCTGCTCC CTATCTGGTC CTGACCACCC TGCTAATCTT CACTGGGGCC TTCCTCCTGG 
     1321 GCTACGTGGC CTTTCGAGGG TCCTGCCAGG CATGTGGGGA CTCTGTGTTG GTGGTTGGTG 
     1381 AAGATGTCAA CTCTGAGGAC TCCAGCCGGG GCACGTTGTA CTGGAGTGAC CTCCAGGACA 
     1441 TGTTTCTCCG GTTCCTTGGG GAGGGACGCA TGGAGGACAC CATCAGGCTG ACCAGCCTCC 
     1501 GGGAACGCGT GGCCGGCTCA GCCAGAATGG CCACCCTGGT CCAAGACATC CTCGATAAGC 
     1561 TCTCGCGCCA GAAGCTGGAC CACGTGTGGA CTGACACGCA CTATGTGGGA CTTCAGTTCC 
     1621 CGGACCCGGC TCACCCTAAC ACCCTGCACT GGGTGGGTGC AGACGGGAGC GTCCAAGAGC 
     1681 AGCTACCGCT GGAGGATCCG GAAGTCTACT GTCCCTACAG CGCCACGGGC AACGCCACGG 
     1741 GCAAGCTTGT GTACGCCCAC TACGGGCGGC GGGAGGACCT GCAGGACCTG AAAGCCAAGG 
     1801 ACGTGGAGCT GGCCGGCAGC CTCCTGCTAG TGCGTGCTGG GATTACAAGC TTCGCCCAGA 
     1861 AGGTAGCCAT TGCCCAGGAC TTTGGGGCCC ACGGAGTGCT GATATACCCT GACCCAGCGG 
     1921 ACTTCTCCCA AGACCCCCAC AAGCCAGGCC TGTCTAGTGA CAGGGCTGTG TATGGACATG 
     1981 TGCACCTGGG AACTGGGGAC CCTTACACGC CTGGCTTCCC GTCCTTCAAT CAAACCCAGT 
     2041 TCCCTCCAGT AGAATCATCG GGGCTTCCCA ACATCCCTGC CCAGCCCATC AGTGCCGACG 
     2101 TTGCTGATCG CTTGCTCAGG AAACTCACAG GTCCCGTGGC TCCTCAGGAA TGGAAGGGTC 
     2161 GCCTCTCAGA CTCTCCGTAT CGCCTGGGAC CTGGGCCAGG CTTACGCCTT GTGGTCAACA 
     2221 ACCACAGAAC CTCTACTCCC ATCAGTAACA TCTTTGCGTG CATCGAGGGC TTCGCAGAGC 
     2281 CAGATCACTA TGTCGTTATC GGGGCCCAGA GGGATGCCTG GGGCCCAGGA GCAGCCAAGT 
     2341 CTGCAGTGGG GACTGCCATC CTCCTGGAGC TGGTTCGGAC CTTTTCCTCC ATGGTCAGCA 
     2401 GTGGCTTTAG ACCTCGAAGA AGTCTTTTGT TCATCAGCTG GGACGGAGGT GACTTTGGCA 
     2461 GCGTGGGAGC CACGGAGTGG TTGGAGGGCT ACCTCAGCGT GCTACACCTC AAAGCTGTCG 
     2521 TGTATGTGAG CCTGGACAAC TCCGTGTTGG GAGACGGCAA ATTCCATGCT AAGACCAGCC 
     2581 CCCTTCTCGT CAGCCTCATT GAGAATATCC TGAAGCAGGT GGATTCCCCT AACCACAGTG 
     2641 GACAGACACT CTACGATCAA GTGGCATTCA CCCACCCAAG CTGGGATGCT GAAGTGATCC 
     2701 AGCCCCTGCC CATGGACAGC AGCGCATATT CCTTCACAGC TTTTGCGGGC GTCCCAGCTG 
     2761 TGGAGTTCTC CTTCATGGAG GACGATAGGG TGTACCCATT CCTGCACACG AAGGAGGACA 
     2821 CGTATGAGAA TCTGCACAAG ATGCTGCGAG GTCGCCTGCC CGCCGTGGTC CTAGCAGTGG 
     2881 CTCAGCTCGC TGGTCAGCTC CTCATCCGAC TGAGCCACGA TCACCTACTG CCGCTGGACT 
     2941 TCGGCCGCTA CGGAGACGTG GTCCTCAGGC ACATCGGCAA CCTTAATGAG TTCTCTGGGG 
     3001 ACCTCAAGGC GCGCGGGCTG ACCCTGCAGT GGGTGTACTC TGCAAGGGGG GACTATATCC 
     3061 GGGCGGCGGA GAAGCTGCGG AAGGAGATTT ACAGCTCGGA GCAGAGCGAT GAGCGTCTGA 
     3121 TGCGCATGTA CAACGTGCGC ATCATGAGGG TGGAGTTCTA CTTCCTGTCC CAGTACGTGT 
     3181 CGCCGGCCGA CTCCCCATTC CGCCACATTT TCCTAGGCCA AGGCGACCAC ACTTTGGGTG 
     3241 CCCTGGTGGA ACACCTACGG ATGCTGCGCT CCGATGGCTC AGGAGCTGCC TCTTCTGGGT 
     3301 TGAGCCCAGG TCTGGGCTTC CAGGAGAGTC GCTTCCGGCG ACAGCTGGCG CTGCTCACGT 
     3361 GGACGCTACA GGGGGCAGCC AACGCACTCA GTGGCGACGT TTGGAACATC GACAATAACT 
     3421 TTAGCGGACC GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3481 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3541 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3601 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3661 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3721 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3781 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3841 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     3901 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     3961 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4021 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4081 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4141 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4201 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4261 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4321 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4381 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4441 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4501 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4561 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4621 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4681 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4741 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4801 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4861 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     4921 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     4981 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5041 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5101 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5161 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5221 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5281 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5341 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5401 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5461 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5521 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5581 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5641 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5701 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5761 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5821 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     5881 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     5941 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6001 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6061 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6121 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6181 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6241 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6301 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6361 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6421 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6481 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6541 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6601 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6661 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6721 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6781 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6841 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     6901 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     6961 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7021 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7081 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7141 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7201 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7261 TAACAAAAAT TTAACGCGAA TTTT
//