LOCUS       RR205616                8820 bp    DNA     circular  SYN 
DEFINITION  Ace (Myc-DDK-tagged ORF) - Rat angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 (Ace), (10 ug)
ACCESSION   NM_012544
VERSION     NM_012544.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4977..5068 
                     /label="Myc-DDK"  
     misc_feature    5098..5683 
                     /label="PolyA signal"  
     misc_feature    5155..5174 
                     /label="Restriction Enzyme"  
     misc_feature    5959..6631 
                     /label="ColE1"  
     misc_feature    7061..7855 
                     /label="Kan/Neo"  
     misc_feature    7890..8192 
                     /label="SV40 ori"  
     misc_feature    8278..8755 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4959..4965 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4959 
                     /label="Ace"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGGCCGCG TCCGGCCAGC GGGGGCGGTG GCCGTTGTCA CCGCCGCTCT 
     1081 TGATGCTGTC GCTGCTGCTG CTGCTGCTGC TGCCGCCGTC GCCCGCCCCG GCGCTTGACC 
     1141 CTGGATTGCA GCCGGGCAAC TTTTCCGCGG ACGAGGCAGG GGCGCAGCTC TTCGCTGACA 
     1201 GCTATAACTC GAGTGCCGAG GTGGTGATGT TCCAGAGCAC CGCAGCCAGC TGGGCGCACG 
     1261 ACACCAACAT CACGGAGGAG AATGCGCGGC TCCAGGAGGA AGCGGCCCTG ATCAACCAGG 
     1321 AGTTTGCAGA GGTCTGGGGC AAGAAGGCCA AGGAGCTGTA TGAGTCCATC TGGCAGAACT 
     1381 TCACTGACCA AAAGCTGCGA AGGATCATCG GATCCGTACA GACCCTAGGA CCTGCCAACC 
     1441 TGCCCCTGAC CCAGCGGCTG CAGTACAACT CTCTGCTAAG CAACATGAGC AGAATCTACT 
     1501 CCACCGGCAA GGTCTGCTTC CCCAACAAGA CTGCCACCTG CTGGTCCCTG GACCCAGAGC 
     1561 TCACCAACAT CCTGGCTTCC TCACGAAACT ATGCCAAGGT GCTGTTTGCC TGGGAAGGCT 
     1621 GGCATGATGC TGTGGGTATC CCACTGAAGC CCCTCTATCA GGACTTTACT GCCCTCAGTA 
     1681 ATGAAGCCTA CAGACAAGAT GGCTTCTCAG ACACAGGAGC CTACTGGCGC TCCTGGTATG 
     1741 AGTCCCCCTC CTTTGAAGAG AGTTTGGAGC ATCTCTACCA CCAAGTCGAG CCCCTCTACC 
     1801 TGAACCTCCA TGCCTTTGTC CGTCGCGCAC TGCACCGCCG CTATGGGGAC AAATACATCA 
     1861 ATCTCAGAGG TCCTATTCCC GCTCATCTGC TGGGAGACAT GTGGGCGCAG AGCTGGGAGA 
     1921 ACATTTACGA CATGGTAGTG CCTTTCCCGG ACAAACCCAA CCTCGATGTC ACCAGTACAA 
     1981 TGGTACAGAA GGGCTGGAAT GCCACGCACA TGTTCCGGGT CGCAGAGGAA TTCTTTACCT 
     2041 CGCTGGGGCT CTCCCCCATG CCTCCAGAGT TCTGGGCGGA GTCGATGCTG GAGAAACCAG 
     2101 CTGATGGACG GGAGGTGGTG TGCCATGCCT CTGCGTGGGA CTTCTACAAC AGGAAGGACT 
     2161 TCAGGATTAA GCAGTGCACG CGGGTCACGA TGGACCAGCT GTCCACAGTA CACCACGAGA 
     2221 TGGGCCACGT GCAGTACTAT CTCCAGTACA AGGACCTGCA CGTCTCTCTG CGTCGAGGTG 
     2281 CCAACCCTGG CTTCCACGAG GCCATCGGGG ATGTACTCGC TCTCTCTGTC TCTACCCCAG 
     2341 CACATCTGCA CAAAATTGGC CTGCTAGACC GTGTTGCCAA TGACATAGAA AGTGACATCA 
     2401 ATTACTTGCT AAAGATGGCC CTAGAGAAAA TTGCCTTCTT GCCCTTTGGT TACCTGGTGG 
     2461 ACCAGTGGCG CTGGGGGGTC TTCAGTGGAC GTACCCCACC CTCTCGCTAC AACTACGACT 
     2521 GGTGGTATCT TCGAACCAAG TATCAGGGGA TCTGCCCACC AGTTGCTCGG AATGAAACCC 
     2581 ATTTTGACGC TGGGGCCAAG TTTCACATCC CAAGCGTGAC ACCATACATC AGGTACTTTG 
     2641 TGAGTTTCGT GCTACAGTTC CAGTTCCATC AAGCGCTGTG CAAGGAGGCA GGCCACCAGG 
     2701 GTCCACTACA CCAGTGTGAC ATCTACCAGT CCACCAAGGC AGGGGCCAAG CTCCAACAGG 
     2761 TGCTGCAGGC TGGCTGCTCC AGGCCCTGGC AGGAGGTGCT GAAGGACCTG GTGGGTTCAG 
     2821 ATGCGCTGGA TGCCAGTGCG CTAATGGAGT ACTTCCAACC AGTAAGCCAG TGGCTGCAGG 
     2881 AGCAGAATCA GCGGAATGGC GAGGTCCTAG GCTGGCCGGA GTATCAGTGG CGTCCACCGT 
     2941 TACCAGACAA CTATCCAGAG GGAATTGACC TAGAGACTGA TGAAGCCAAG GCTAACAGGT 
     3001 TCGTGGAGGA GTATGACCGG ACAGCCAAGG TGTTGTGGAA CGAATACGCA GAGGCCAACT 
     3061 GGCATTATAA CACCAACATT ACCATAGAGG GCAGCAAGAT CCTGCTTCAG AAAAACAAGG 
     3121 AAGTGTCCAA CCATACCTTG AAATATGGCA CCTGGGCCAA GACATTTGAC GTGAGCAACT 
     3181 TCCAGAACTC TACCATCAAG CGGATCATAA AGAAGGTTCA GAACGTGGAC CGGGCAGTGC 
     3241 TGCCTCCCAA CGAGTTAGAA GAGTACAACC AGATCCTGCT AGACATGGAG ACGACTTACA 
     3301 GTGTAGCCAA TGTTTGCTAC ACAAATGGCA CTTGTCTGTC ACTGGAGCCT GATCTGACAA 
     3361 ATATAATGGC CACGTCCCGG AAATACGAAG AATTGCTTTG GGTGTGGAAG AGCTGGCGAG 
     3421 ACAAGGTGGG GAGAGCCATC CTTCCCTTTT TCCCAAAGTA CGTGGACTTC TCCAACAAGA 
     3481 TCGCCAAGCT CAACGGCTAC TCTGATGCAG GGGATTCCTG GAGATCCTCA TATGAGTCCG 
     3541 ACGACTTGGA GCAAGACCTG GAAAAACTAT ACCAGGAGCT GCAGCCGCTC TACCTGAACC 
     3601 TGCATGCCTA TGTGCGCCGC TCCCTGCACC GCCATTATGG GTCTGAGTAC ATCAACCTGG 
     3661 ATGGTCCCAT TCCTGCTCAC CTGCTAGGGA ACATGTGGGC ACAGACTTGG TCCAACATCT 
     3721 ATGACTTGGT GGCACCCTTC CCTTCCGCCC CCAGTATAGA TGCCACGGAG GCCATGATAA 
     3781 AGCAGGGATG GACACCCAGA AGGATATTTA AGGAAGCTGA CAATTTTTTT ACCTCCCTGG 
     3841 GGCTGTTACC TGTGCCCCCT GAGTTCTGGA ACAAGTCAAT GTTAGAGAAG CCAACCGATG 
     3901 GGAGGGAGGT GGTGTGCCAT GCCTCAGCCT GGGACTTCTA CAACGGCAAG GACTTCAGGA 
     3961 TCAAGCAGTG TACCTCTGTG AACATGGAGG AATTGGTGAT AGCCCACCAC GAAATGGGCC 
     4021 ACATCCAGTA TTTCATGCAG TACAAAGACT TGCCTGTGAC CTTTCGGGAG GGCGCCAACC 
     4081 CCGGTTTTCA TGAGGCTATT GGAGATGTTT TGGCTCTGTC TGTGTCTACA CCCAAGCATC 
     4141 TACACAGTCT CAACCTGCTC AGCAGTGAGG GCAGTGGCTA CGAGCATGAC ATCAACTTTC 
     4201 TAATGAAGAT GGCCCTTGAC AAGATCGCCT TCATCCCCTT CAGCTACCTC ATTGACCAGT 
     4261 GGCGCTGGAG GGTCTTTGAC GGAAGCATCA CCAAGGAGAA CTACAACCAG GAGTGGTGGA 
     4321 GTCTCAGACT GAAGTACCAG GGTCTCTGCC CTCCAGTGCC TAGATCCCAA GGTGACTTTG 
     4381 ACCCAGGGTC CAAGTTCCAC GTTCCTGCGA ATGTGCCATA CATCAGGTAC TTTATCAGCT 
     4441 TCATCATCCA GTTCCAGTTC CACGAGGCAC TATGTCGCGC AGCCGGGCAC ACCGGCCCCC 
     4501 TGTACAAGTG TGATATCTAC CAATCCAAGG AAGCAGGGAA GCTGCTGGCA GATGCCATGA 
     4561 AGTTGGGCTA CAGTAAGCAG TGGCCAGAAG CCATGAAGAT AATCACAGGC CAACCTAACA 
     4621 TGTCAGCCTC TGCCATTATG AATTACTTCA AGCCACTGAC TGAATGGCTC GTCACAGAGA 
     4681 ACAGGAGACA TGGAGAGACA CTGGGCTGGC CGGAGTACAC CTGGACACCA AACACGGCTC 
     4741 GTGCAGAAGG CTCCCTCCCA GAGTCCAGTC GCGTCAACTT CCTGGGTATG TACCTGGAAC 
     4801 CACAGCAGGC CCGTGTGGGC CAGTGGGTGC TGCTCTTCCT AGGCGTCGCC CTGCTGGTGG 
     4861 CCACCGTGGG TCTCGCCCAC CGACTCTACA ACATCCATAA CCATCACAGC CTCCGCCGGC 
     4921 CCCACCGTGG GCCCCAGTTT GGGTCCGAGG TGGAGCTCAG ACACTCCACG CGTACGCGGC 
     4981 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     5041 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     5101 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     5161 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5221 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5281 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5341 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5401 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5461 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5521 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5581 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5641 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5701 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5761 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5821 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5881 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5941 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     6001 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     6061 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     6121 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6181 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6241 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6301 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6361 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6421 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6481 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6541 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6601 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6661 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6721 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6781 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6841 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6901 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6961 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     7021 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     7081 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     7141 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7201 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7261 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7321 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7381 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7441 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7501 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7561 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7621 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7681 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7741 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7801 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7861 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7921 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7981 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     8041 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     8101 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     8161 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8221 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8281 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8341 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8401 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8461 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8521 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8581 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8641 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8701 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8761 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//