LOCUS       RR205561                9008 bp    DNA     circular  SYN 
DEFINITION  Flt4 (Myc-DDK-tagged ORF) - Rat fms-related tyrosine kinase 4 (Flt4), (10 ug)
ACCESSION   NM_053652
VERSION     NM_053652.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5163..5254 
                     /label="Myc-DDK"  
     misc_feature    5284..5869 
                     /label="PolyA signal"  
     misc_feature    5341..5360 
                     /label="Restriction Enzyme"  
     misc_feature    6145..6817 
                     /label="ColE1"  
     misc_feature    7247..8041 
                     /label="Kan/Neo"  
     misc_feature    8076..8378 
                     /label="SV40 ori"  
     misc_feature    8464..8941 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5138..5145 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5138 
                     /label="Flt4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGCCGGGC GCTGCGCTGA ACCGGCGCCT GTGGCTCTGC CTCGGACTCC 
     1081 TCCAAGGCCT GGCAAATGGC TACTCCATGA CCCCTCCAAC TCTGAACATC ACAGAGGATT 
     1141 CATATGTCAT TGACACCGGG GACAGCCTAT CCATATCCTG CAGGGGGCAG CACCCCCTCG 
     1201 AGTGGACCTG GCGAGGGGCC CAAGAGGTAC TGACCACAGG TGGGAAGGAC AGCGAGGACA 
     1261 CGCAGGTTGT GCAAGACTGT GAAGGCACAG AAGCTAGGCC CTACTGCAAG GTGCTGTCGC 
     1321 TGGCCCAGAC TCACGCCAAC AACACGGGCA GCTATTACTG CTACTACAAG TATATCAAGG 
     1381 CCCGGATTGA GGGCACCACA GCTGCCAGCA CCTATGTGTT TGTAAGAGAC TTTGAACAGC 
     1441 CTTTCATCAA CAAACCTGAC ACGCTCCTGG TCAACAGGAA GGACTCGATG TGGGTGCCCT 
     1501 GCTTGGTGTC CATTCCCGGC CTCAACATCA CACTGCGCTC GCAAAGTTCG GTGCTGCACC 
     1561 CTGATGGGCA GGAGGTGTTG TGGGACGACC GCCGGGGCAT GCGGGTGCCC ACTCTACTGT 
     1621 TGCGTGACGC CCTGTACCTG CAGTGCGAGA CCACCTGGGG AGACCAGGAC TTCCTTTCCA 
     1681 ATCCCTTCCT CGTGCACATC ACAGGCAATG AGCTCTATGA CATCCAGCTG TACCCCAAGA 
     1741 AGTCACTGGA GCTGTTGGTT GGAGAGAAGC TGGTTTTGAA CTGTACAGTA TGGGCTGAGT 
     1801 TCGACTCTGG TGTCACCTTC GACTGGGATT ACCCAGGAAA GCAGGCAGAG CGTGCTAAGT 
     1861 GGGTACCTGA GCGGCGTTCC CAGCAGACCC ACACGGAACT CTCCAGCATC CTGACCATCC 
     1921 ACAATGTCAG CCAGCACGAC CTGGGCCCCT ATGTGTGTGA GGCCAACAAT GGGATTCAGC 
     1981 AGTTCCGGGA AAGCACGGAG GTCATTGTGC ACGAAAAGCC CTTCATCAGT GTCGAGTGGC 
     2041 TCAAAGGACC TGTCCTGGAG GCCACAGCCG GTGACGAGAT GGTGAAGCTA CCCGTGAAGC 
     2101 TTGCAGCTTA TCCCCCACCG GAGTTCCAAT GGTACAAGGA CAGAAAGGCA GTGACTGGGC 
     2161 GCCACAATCC ACATGCTCTG GTGCTCAAAG AGGTGACCGA GGCCAGTGCA GGCGTCTACA 
     2221 CCCTCGCCCT GTGGAACTCT GCAGCCGGTC TGAGGCAGAA TATCAGTCTG GAGCTGGTGG 
     2281 TGAATGTGCC GCCCCACATC CATGAAAAGG AAGCTTCTTC ACCCAGCATC TACTCCCGCC 
     2341 ACAGCCGCCA GACCCTCACC TGCACCACCT ATGGGGTACC CCAACCTCTC AGTGTTCAGT 
     2401 GGCACTGGAG GCCCTGGACA CCCTGTAAGA CATTTGCCCA GCGCAGTCTC CGGAGGCGGC 
     2461 AGCCGCGGGA TGGCATGCCA CAGTGCCGAG ACTGGAAGGA GGTGACCACT CAGGACGCTG 
     2521 TGAACCCCAT CGAGAGCCTG GACACCTGGA CCGAGTCTGT GGAGGGCAAG AACAAGACGG 
     2581 TGAGCAAGCT GGTGATTCAG GATGCCAATG TGTCAGCCAT GTACAAATGC GTGGTCTTCA 
     2641 ACAAAGTGGG CCAGGACGAG CGGCTCATCT ACTTCTATGT GACCACCATC CCTGACGGCT 
     2701 TCAGTATCGA ATCAGAGCCA TCCGAGGATC CCTTAGAAGG CCAGTCCGTG CGCCTCAGCT 
     2761 GCCGGGCGGA CAACTACACG TATGAGCATC TGCGCTGGTA CCGGCTCAAC CTCTCCACGC 
     2821 TGCACGACGC TCAAGGGAAC CCCCTACTGC TCGACTGCAA GAACGTGCAC CTGTTTGCCA 
     2881 CGCCCCTAGA GGCCAACCTA GAGGAGGCGG AGCCTGGGGC CCGCCACGCC ACCCTCAGCT 
     2941 TGAATATCCC CCGAGTGGCG CCCGAGGACG AGGGTGACTA CGTGTGTGAA GTGCAGGATA 
     3001 GGCGCAGCCA GGACAAGCAC TGCCACAAGA AGTACCTGTC CGTGCAGGCC CTGGAAGCTC 
     3061 CTCGGCTCAC GCAGAACTTA ACCGACCTCC TGGTGAACGT GAGAACCTCC CTGGAGATGC 
     3121 GATGTCCGGT AGCCGGAGCA CATGTGCCCA GTATTGTGTG GTACAAAGAT GAAAGGCTCC 
     3181 TGGAGAAAGA GTCGGGAATC GACCTGGCAG ACTCCAATCA GAGGCTGAGC ATCCAACGCG 
     3241 TGCGCGAGGA GGACGCAGGT CGTTACCTGT GCAGTGTGTG CAATGCCAAG GGCTGCGTAA 
     3301 ACTCCTCCGC CAGCGTGGCC GTAGAAGGCT CGGAAGATAA AGGCAGCATG GAGATTGTGA 
     3361 TACTCATTGG CACTGGCGTC ATCGCAGTCT TCTTCTGGGT CCTCCTCCTG CTCATCTTCT 
     3421 GTAACATGAA AAGGCCTGCC CATGCAGACA TCAAGACGGG CTACCTGTCC ATCATCATGG 
     3481 ACCCCGGGGA GGTGCCTTTG GAGGAGCAGT GTGAATACCT GTCCTATGAC GTCAGCCAGT 
     3541 GGGAGTTCCC CAGGGAAAGG TTGCACCTCG GGAGAGTCCT AGGCCATGGG GCTTTTGGGA 
     3601 AGGTGGTGGA AGCCTCAGCC TTTGGCATCA ATAAAGGCAG CAGCTGTGAT ACCGTGGCGG 
     3661 TGAAGATGCT GAAAGAGGGC GCTACTGCCA GCGAACACCG TGCCCTGATG TCCGAGCTCA 
     3721 AGATCCTAAT TCACATCGGT AACCACCTCA ATGTGGTCAA CCTCCTAGGG GCGTGCACCA 
     3781 AGCCCAATGG CCCTCTCATG GTGATCGTGG AGTTTTGCAA ATACGGCAAC CTCTCCAACT 
     3841 TCTTGCGTGT CAAGCGGGAG ACGTTCGACC CCTACGCAGA GAAGTCTCCC GAGCAACGCA 
     3901 GGCGCTTCCG CGCGATGGTA GAAGGCGCCA AGGCTGATAG GAGGAGACTT GGAAGCACCG 
     3961 ACAGAGCCCT GTTTACAAGG TTCCTGATGG GCAAAGGAAG TGCACGGCGA GCCCCATTTG 
     4021 TCCAAGAAGC TGAGGACCTG TGGTTGAGCC CGCTGACCAT GGAGGACCTT GTCTGCTACA 
     4081 GTTTCCAAGT GGCCCGGGGA ATGGAGTTCC TGGCTTCCCG CAAGTGCATT CACAGAGACC 
     4141 TGGCTGCTCG GAACATTTTA CTGTCAGAAA GTGACATAGT GAAGATCTGT GACTTTGGCC 
     4201 TCGCTCGGGA CATCTACAAA GACCCTGACT ATGTCCGAAA GGGCAGCGCC CGACTGCCTC 
     4261 TGAAATGGAT GGCCCCCGAG AGCATCTTTG ATAAGGTGTA CACCACGCAG AGTGATGTGT 
     4321 GGTCCTTCGG CGTGCTGCTG TGGGAGATCT TCTCATTGGG GGCCTCTCCA TACCCTGGGG 
     4381 TACAGATCAA TGAGGAATTC TGCCAGCGGC TGAAAGACGG CACACGAATG AGGGCCCCAG 
     4441 AACTGGCCAC TCCTGCCATA CGCCACATCA TGCAGAGTTG CTGGTCTGGA GACCCTAAAG 
     4501 CGAGGCCTGC TTTCTCTGAC CTAGTGGAGA TCCTGGGGGA CCTGCTTCAG GGTGGAGGCT 
     4561 GGCAGGAGGA GGAGGAGGAA TGCATGGCCC TGCACAGTTC TCAGAGCTCA GAGGAGGATG 
     4621 GCTTCATGCA GGCATCCACC ACAGCTCTAC ATATCACGGA GGCTGACGCT GAGAGCAGTC 
     4681 CACCCAGCAT GCATTGCCAC AGCCTGGCAG CCAGATATTA CAACTGTGTG TCCTTTCCTG 
     4741 GGCGCCTGGT CAGGGGGACT AAGGCTCCAG GTTCCTCCAG GATGAAGACG TTTGAAGAAT 
     4801 TGCCCATGAC CCCTACAACC TACAAAGCCT CTGTGGATAA CCAAACAGAC AGCGGGATGG 
     4861 TGCTGGCCTC AGAAGAGTTT GAGCAGATAG AAAGCAGGCA TAGACAAGAA GGCAGCTTCA 
     4921 GCCGTAAAGA TCCTGGCCAG CACATGGATA TTTCCAGAGG ACACCCGGAC CTCCAGGGGA 
     4981 GGCGGCGACG GCCCACCCAG GGGGCACAAG GAGGCAAGGT GTTTTACAAC AACGAGTATG 
     5041 GGGAGGTCTC CCAGCCGTGT ACAGAAGGTG ACTGCTGCCC GTCTGCTGGC TCCACCTTCT 
     5101 TCGCAGACAG CAACTACGCT CCACCTTCTT CGCAGACAGC AACTACAGCG GACCGACGCG 
     5161 TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT 
     5221 GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT 
     5281 GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG 
     5341 TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG 
     5401 ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG 
     5461 TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG 
     5521 CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC 
     5581 CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT 
     5641 GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA 
     5701 TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC 
     5761 CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC 
     5821 TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT 
     5881 GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA 
     5941 TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG 
     6001 AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG 
     6061 TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG 
     6121 TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG 
     6181 CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA 
     6241 AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC 
     6301 TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT 
     6361 AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT 
     6421 GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG 
     6481 CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT 
     6541 ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT 
     6601 GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT 
     6661 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG 
     6721 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT 
     6781 AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG 
     6841 TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA 
     6901 ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG 
     6961 GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT 
     7021 ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC 
     7081 CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG 
     7141 GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA 
     7201 TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC 
     7261 AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC 
     7321 CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG 
     7381 TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC 
     7441 CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA 
     7501 CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA 
     7561 GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC 
     7621 GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG 
     7681 TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG 
     7741 ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG 
     7801 TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG 
     7861 CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG 
     7921 GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG 
     7981 CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT 
     8041 CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT 
     8101 AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG 
     8161 GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC 
     8221 GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA 
     8281 TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT 
     8341 GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA 
     8401 CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC 
     8461 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 
     8521 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 
     8581 CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA 
     8641 CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT 
     8701 TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG 
     8761 CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT 
     8821 CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC 
     8881 TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG 
     8941 GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG 
     9001 CGAATTTT
//