LOCUS       RR205559L3                8489 bp    DNA     circular  SYN 
DEFINITION  Samd8 (Myc-DDK-tagged ORF) - Rat sterile alpha motif domain containing 8 (Samd8), (10 ug)
ACCESSION   NM_001012040
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    6780..6960 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    7688..8347 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7008..7627 
                     /label="ColE1"  
     misc_feature    4820..4843 
                     /label="DDK"  
     misc_feature    5655..5675 
                     /label="LR50"  
     misc_feature    4772..4843 
                     /label="MYC"  
     misc_feature    4853..4918 
                     /label="P2A"  
     misc_feature    4919..5518 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    5664..6255 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    4754..4760 
                     /label="Restriction Enzyme"  
     misc_feature    3320..4754 
                     /label="Samd8"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GCCCGCGCGT AGCCGAGCCG GCTCCCGCCA 
     3361 GCGCTCCGGC TCCCTGCCCC GGCCTTCGCG TCTGCCTCTT GAGGCGCTGA GCTCCGCCTA 
     3421 CGCGCCGAGG ACCCCGGACT CCGACGGCGC CATAGACACC GGCTCCGAGC TGGGTCCCGG 
     3481 CAGCCCGGCT CCCACCGCGG AGGAAGTAGA AGAGGAAATG GCAGGCCCTA GTCAACTCTG 
     3541 CATTCGCCGC TGGACTCCCA GGCATGTGGC CGTGTGGCTG AAGGAGGAGG GGTTTTTTGA 
     3601 GTATGTGGAC ATCTTATGTA ACAAGCACCG TCTGGATGGA GTCACACTGC TCACGCTCAC 
     3661 CGAGTATGAT CTCCGCTCTC CTCCTCTGGA AATCAAAGTC CTAGGAGACA TTAAACGGCT 
     3721 AATGCTCTCA GTCCGAAAAC TGCAGAAAAC ACACATTGAT GTTTTGGAAG AGATGGGTTA 
     3781 CAACAGTGAC AGTCCCATAA GTCCCATGAC GCCATTCATC AGTGCCCTTC AGAGTGCAGA 
     3841 CTGGCTCTGT AATGGAGAGC CTGCACACAG CTGTGATGGG CCCATCCCTG ATCTGAGCTC 
     3901 TGATCAGTAC CAATACATGA ATGGCAAGAA CAAGCACTGT GCCCGAAGAC TGGACCCCGA 
     3961 GTACTGGAAG ACCGTCCTGA GCTGTGTATA TGTCTTTATA GTATTTGGGT TCACCTCTTT 
     4021 CATTATGGTC ATTGTTCACG AGCGAGTGCC TGACATGCAG ACCTACCCAC CACTCCCAGA 
     4081 TATATTCTTA GACAGTGTTC CTAGGATCCC GTGGGCCTTC TCCATGACTG AAGTGTGTGG 
     4141 CATGATCCTG TGCTACATCT GGATCCTGGT CCTCCTCCTT CACAAGCACA GGTCAATCCT 
     4201 TCTGCGAAGG CTCTGTAGTC TGATGGGCAC TGTATTCTTG CTTCGCTGCT TTACCATGTT 
     4261 TGTGACCTCC CTCTCCGTGC CAGGACAGCA CCTGCAGTGT ACTGGAAAGG TGTATGGAAG 
     4321 TGTGTGGGAG AAACTGCATC GGGCGTTTGC CATTTGGAGT GGCTTCGGCA TGACTCTGAC 
     4381 GGGCGTGCAC ACTTGTGGGG ATTACATGTT CAGTGGCCAC ACCGTTGTCC TAACCATGCT 
     4441 GAATTTCTTT GTCACAGAAT ATACGCCAAG AAGCTGGAAT TTCTTGCACA CTCTCTCGTG 
     4501 GGTTCTCAAC CTCTTTGGAA TCTTCTTCAT CTTGGCTGCC CATGAACATT ATTCCATTGA 
     4561 CGTGTTTATT GCCTTTTATA TCACAACAAG ACTCTTTTTG TACTACCACA CTCTGGCTAA 
     4621 TACCAGAGCG TATCACCAGA GCAGGAGAGC GAGAATTTGG TTCCCTATGT TTTCTTTCTT 
     4681 TGAATGCAAT GTTAATGGCA CAGTACCTAA TGAATATTGT TGGCCATTTT CTAAACCAGC 
     4741 AATAATGAAA AGACTAATTG GAACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA 
     4801 AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTGGGT 
     4861 AGGAAGCGGA GCTACTAACT TCAGCCTGCT GAAGCAGGCT GGAGACGTGG AGGAGAACCC 
     4921 TGGACCTATG ACCGAGTACA AGCCCACGGT GCGCCTCGCC ACCCGCGACG ACGTCCCCAG 
     4981 GGCCGTACGC ACCCTCGCCG CCGCGTTCGC CGACTACCCC GCCACGCGCC ACACCGTCGA 
     5041 TCCGGATCGC CACATCGAGC GGGTCACCGA GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG 
     5101 GCTCGACATC GGCAAGGTGT GGGTCGCGGA CGACGGCGCC GCGGTGGCGG TCTGGACCAC 
     5161 GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT CGCCGAGATC GGCCCGCGCA TGGCCGAGTT 
     5221 GAGCGGTTCC CGGCTGGCCG CGCAGCAACA GATGGAAGGC CTCCTGGCGC CGCACCGGCC 
     5281 CAAGGAGCCC GCGTGGTTCC TGGCCACCGT CGGCGTCTCG CCCGACCACC AGGGCAAGGG 
     5341 TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC 
     5401 CTTCCTGGAG ACCTCCGCGC CCCGCAACCT CCCCTTCTAC GAGCGGCTCG GCTTCACCGT 
     5461 CACCGCCGAC GTCGAGGTGC CCGAAGGACC GCGCACCTGG TGCATGACCC GCAAGCCCGG 
     5521 TGCCTGATGT ACACCCAAAC GGCCGGCCGC GGTCTGTACA AGTAGGATTC GTCGAGGGAC 
     5581 CTAATAACTT CGTATAGCAT ACATTATACG AAGTTATACA TGTTTAAGGG TTCCGGTTCC 
     5641 ACTAGGTACA ATTCGATATC AAGCTTATCG ATAATCAACC TCTGGATTAC AAAATTTGTG 
     5701 AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT 
     5761 TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA 
     5821 AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG 
     5881 TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC 
     5941 TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT 
     6001 GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT 
     6061 CGGGGAAATC ATCGTCCTTT CCTTGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG 
     6121 GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC 
     6181 TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT 
     6241 CCCTTTGGGC CGCCTCCCCG CATCGATACC GTCGACCTCG ATCGAGACCT AGAAAAACAT 
     6301 GGAGCAATCA CAAGTAGCAA TACAGCAGCT ACCAATGCTG ATTGTGCCTG GCTAGAAGCA 
     6361 CAAGAGGAGG AGGAGGTGGG TTTTCCAGTC ACACCTCAGG TACCTTTAAG ACCAATGACT 
     6421 TACAAGGCAG CTGTAGATCT TAGCCACTTT TTAAAAGAAA AGGGGGGACT GGAAGGGCTA 
     6481 ATTCACTCCC AACGAAGACA AGATATCCTT GATCTGTGGA TCTACCACAC ACAAGGCTAC 
     6541 TTCCCTGATT GGCAGAACTA CACACCAGGG CCAGGGATCA GATATCCACT GACCTTTGGA 
     6601 TGGTGCTACA AGCTAGTACC AGTTGAGCAA GAGAAGGTAG AAGAAGCCAA TGAAGGAGAG 
     6661 AACACCCGCT TGTTACACCC TGTGAGCCTG CATGGGATGG ATGACCCGGA GAGAGAAGTA 
     6721 TTAGAGTGGA GGTTTGACAG CCGCCTAGCA TTTCATCACA TGGCCCGAGA GCTGCATCCG 
     6781 GACTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC TGGCTAACTA 
     6841 GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC 
     6901 CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA 
     6961 ATCTCTAGCA GCATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG 
     7021 TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA 
     7081 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC 
     7141 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC 
     7201 CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG 
     7261 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC 
     7321 TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA 
     7381 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG 
     7441 AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG 
     7501 AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT 
     7561 GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA 
     7621 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA 
     7681 GGGATTTTGG TCATGATTAC GCCCCGCCCT GCCACTCATC GCAGTACTGT TGTAATTCAT 
     7741 TAAGCATTCT GCCGACATGG AAGCCATCAC AAACGGCATG ATGAACCTGA ATCGCCAGCG 
     7801 GCATCAGCAC CTTGTCGCCT TGCGTATAAT ATTTGCCCAT GGTGAAAACG GGGGCGAAGA 
     7861 AGTTGTCCAT ATTGGCCACG TTTAAATCAA AACTGGTGAA ACTCACCCAG GGATTGGCTG 
     7921 AGACGAAAAA CATATTCTCA ATAAACCCTT TAGGGAAATA GGCCAGGTTT TCACCGTAAC 
     7981 ACGCCACATC TTGCGAATAT ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG TATTCACTCC 
     8041 AGAGCGATGA AAACGTTTCA GTTTGCTCAT GGAAAACGGT GTAACAAGGG TGAACACTAT 
     8101 CCCATATCAC CAGCTCACCG TCTTTCATTG CCATACGGAA CTCCGGATGA GCATTCATCA 
     8161 GGCGGGCAAG AATGTGAATA AAGGCCGGAT AAAACTTGTG CTTATTTTTC TTTACGGTCT 
     8221 TTAAAAAGGC CGTAATATCC AGCTGAACGG TCTGGTTATA GGTACATTGA GCAACTGACT 
     8281 GAAATGCCTC AAAATGTTCT TTACGATGCC ATTGGGATAT ATCAACGGTG GTATATCCAG 
     8341 TGATTTTTTT CTCCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 
     8401 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 
     8461 GCACATTTCC CCGAAAAGTG CCACCTGAC
//