LOCUS       RR205534                8415 bp    DNA     circular  SYN 
DEFINITION  Pc (Myc-DDK-tagged ORF) - Rat pyruvate carboxylase (Pc), nuclear gene encoding mitochondrial protein, (10 ug)
ACCESSION   NM_012744
VERSION     NM_012744.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4572..4663 
                     /label="Myc-DDK"  
     misc_feature    4693..5278 
                     /label="PolyA signal"  
     misc_feature    4750..4769 
                     /label="Restriction Enzyme"  
     misc_feature    5554..6226 
                     /label="ColE1"  
     misc_feature    6656..7450 
                     /label="Kan/Neo"  
     misc_feature    7485..7787 
                     /label="SV40 ori"  
     misc_feature    7873..8350 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4554..4560 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4554 
                     /label="Pc"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGAAGTTC CAAACAGTTC GAGGGGGCCT GAGGCTCCTG GGTGTCCGCC 
     1081 GATCCTCCAC AGCCCCCGTT GCCTCCCCAA ATGTCCGGCG TCTGGAGTAC AAGCCCATCA 
     1141 AGAAAGTAAT GGTGGCCAAC AGAGGTGAGA TTGCCATCCG AGTGTTTCGT GCCTGCACAG 
     1201 AGCTGGGTAT CCGCACAGTG GCTGTCTACT CGGAGCAGGA CACAGGGCAG ATGCACCGGC 
     1261 AGAAAGCTGA TGAAGCCTAC CTTATTGGCC GTGGGCTGGC TCCTGTGCAA GCCTACCTGC 
     1321 ACATTCCAGA CATCATTAAG GTGGCCAAGG AGAATGGTGT AGATGCTGTG CACCCTGGCT 
     1381 ATGGGTTCCT CTCAGAGAGA GCAGACTTTG CCCAGGCCTG CCAAGATGCT GGAGTCCGAT 
     1441 TCATTGGTCC AAGCCCAGAG GTGGTCCGCA AGATGGGAGA CAAGGTGGAA GCCCGGGCCA 
     1501 TTGCCATTGC TGCAGGCGTT CCAGTGGTCC CTGGCACTAA TTCCCCCATC AATTCCCTGC 
     1561 ATGAGGCACA CGAGTTCTCT AACACCTATG GTTTCCCTAT TATCTTCAAG GCTGCCTATG 
     1621 GAGGTGGGGG CCGTGGCATG AGGGTTGTGC ATAGCTACGA GGAGCTGGAA GAGAATTACA 
     1681 CCCGGGCCTA CTCTGAGGCC TTGGCAGCCT TTGGGAATGG GGCATTGTTT GTGGAGAAAT 
     1741 TCATTGAGAA GCCAAGACAC ATTGAGGTGC AGATCCTAGG GGACCAATAT GGGAACATCT 
     1801 TGCACTTGTA TGAGCGGGAC TGCTCCATCC AGCGGCGGCA CCAGAAGGTC GTCGAGATTG 
     1861 CCCCTGCTAC CCACCTGGAC CCCCAACTTC GGTCACGCCT CACCAGTGAC TCTGTCAAAC 
     1921 TTGCCAAGCA GGTTGGCTAT GAGAATGCAG GCACTGTCGA GTTCCTGGTG GACAAGCATG 
     1981 GCAAGCACTA CTTCATCGAG GTCAATTCCC GCCTGCAGGT GGAGCACACG GTCACTGAGG 
     2041 AGATTACAGA TGTGGACCTG GTCCATGCTC AGATCCATGT CTCCGAAGGC CGGAGCCTGC 
     2101 CTGACCTGGG GCTGCGGCAG GAAAACATCC GAATCAATGG TTGTGCCATT CAGTGTCGGG 
     2161 TCACCACTGA GGACCCTGCA CGCAGCTTCC AGCCAGACAC TGGCCGCATT GAGGTTTTCC 
     2221 GGAGTGGTGA GGGCATGGGC ATCCGCCTGG ACAATGCCTC AGCATTCCAG GGAGCTGTCA 
     2281 TATCCCCCCA CTATGACTCC CTGCTGGTCA AAGTCATTGC CCATGGCAAA GACCACCCTA 
     2341 CAGCTGCCAC CAAGATGAGC AGAGCCCTGG CGGAGTTCCG TGTCCGAGGT GTAAAGACCA 
     2401 ACATCCCCTT CCTGCAGAAT GTTCTCAACA ACCAGCAGTT CCTAGCGGGC ATTGTGGACA 
     2461 CCCAGTTCAT CGATGAGAAC CCCGAGCTGT TCCAGCTGCG GCCTGCACAG AACCGGGCCC 
     2521 AGAAGTTGCT ACATTACCTT GGACACGTCA TGGTCAATGG CCCTACCACT CCAATCCCCG 
     2581 TCAAGGTCAG TCCCAGCCCT GTGGACCCCA TTGTTCCTGT GGTGCCCATA GGCCCACCCC 
     2641 CAGCTGGTTT CAGAGACATC CTTCTGCGAG AGGGGCCAGA GGGCTTTGCC AGAGCTGTGC 
     2701 GGAATCACCA GGGGCTGCTG CTAATGGACA CAACCTTCCG GGATGCCCAC CAGTCACTAC 
     2761 TTGCCACTAG AGTGCGCACA CACGATCTCA AAAAGATTGC ACCCTACGTT GCCCACAACT 
     2821 TCAACAACCT CTTCAGCATA GAGAACTGGG GAGGAGCCAC ATTTGACGTG GCCATGCGCT 
     2881 TCTTGTATGA GTGCCCCTGG CGGCGGCTCC AGGAGCTCCG GGAGCTCATC CCCAACATCC 
     2941 CATTCCAGAT GCTACTGAGG GGGGCCAATG CTGTGGGCTA CACCAACTAC CCTGACAACG 
     3001 TGGTCTTCAA GTTCTGTGAG GTGGCCAAAG AGAATGGCAT GGACGTCTTC CGGATCTTTG 
     3061 ACTCCCTTAA CTACCTGCCA AACATGCTGC TGGGCATGGA AGCAGCTGGC AGTGCTGGGG 
     3121 GTGTGGTGGA AGCTGCCATC TCCTACACGG GTGACGTGGC TGACCCCAGT CGCACTAAAT 
     3181 ACTCACTGGA GTACTACATG GGCTTAGCTG AAGAACTGGT GCGAGCCGGC ACTCACATCC 
     3241 TCTGCATTAA GGACATGGCA GGCCTGCTGA AGCCTGCAGC ATGCACCATG CTGGTCAGCT 
     3301 CCCTCCGGGA CCGGTTCCCC GACCTCCCAC TGCACATCCA TACCCATGAC ACATCAGGGT 
     3361 CAGGTGTGGC AGCCATGTTG GCCTGTGCAC AAGCTGGGGC TGATGTTGTG GATGTGGCAG 
     3421 TCGACTCTAT GTCTGGGATG ACCTCACAGC CCAGCATGGG GGCCCTGGTG GCCTGTACCA 
     3481 AAGGGACTCC TCTGGACACA GAGGTACCCC TGGAGCGTGT GTTTGACTAC AGTGAGTATT 
     3541 GGGAAGGGGC TCGGGGGCTG TATGCAGCCT TTGATTGCAC GGCTACCATG AAGTCTGGCA 
     3601 ACTCAGACGT GTATGAGAAT GAGATCCCAG GGGGCCAGTA CACCAACCTA CACTTCCAGG 
     3661 CCCACAGCAT GGGACTTGGC TCCAAGTTCA AGGAGGTCAA GAAGGCCTAT GTGGAGGCTA 
     3721 ACCAGATGCT GGGGGACCTC ATCAAGGTGA CACCATCCTC CAAGATTGTG GGGGATCTGG 
     3781 CCCAGTTCAT GGTGCAGAAC GGGTTGAGCC GGGCAGAGGC AGAAGCTCAG GCAGAAGAGC 
     3841 TGTCCTTCCC CCGCTCTGTG GTGGAGTTCC TCCAGGGCTA CATTGGCATT CCCCATGGGG 
     3901 GTTTCCCTGA ACCCTTCCGT TCTAAGGTGC TAAAGGACCT GCCAAGAATA GAAGGGAGGC 
     3961 CTGGAGCCTC CCTCCCTCCC TTGAACCTGA AGGAGCTGGA GAAGGACCTG ATTGATAGGC 
     4021 ATGGAGAGGA GGTGACCCCA GAGGACGTTC TCTCTGCAGC CATGTACCCT GATGTCTTTG 
     4081 CTCAGTTCAA AGACTTCACG GCTACCTTTG GCCCCCTGGA TAGCCTGAAT ACTCGTCTCT 
     4141 TTCTTCAAGG ACCCAAAATT GCAGAGGAGT TTGAGGTTGA GCTGGAACGG GGCAAGACCT 
     4201 TGCACATCAA AGCCCTGGCT GTAAGCGACC TGAACCGTGC TGGCCAGAGG CAGGTGTTCT 
     4261 TTGAACTCAA TGGGCAGCTT CGATCCATTC TGGTTAAAGA CACCCAGGCC ATGAAGGAGA 
     4321 TGCACTTCCA TCCCAAGGCC TTGAAGGATG TGAAGGGCCA AATTGGGGCC CCTATGCCTG 
     4381 GGAAGGTCAT AGACGTCAAG GTGGCAGCAG GAGCCAAGGT GGTTAAGGGC CAGCCCCTCT 
     4441 GTGTGCTCAG CGCCATGAAG ATGGAGACTG TGGTGACTTC GCCCATGGAG GGCACTATCC 
     4501 GAAAGGTTCA CGTGACCAAG GACATGACTC TGGAAGGCGA TGACCTCATC CTAGAGATTG 
     4561 AGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4621 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4681 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4741 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4801 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4861 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4921 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4981 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5041 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5101 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5161 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5221 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5281 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5341 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5401 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5461 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5521 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5581 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5641 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5701 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5761 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5821 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5881 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5941 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6001 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6061 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6121 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6181 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6241 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6301 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6361 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6421 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6481 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6541 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6601 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6661 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6721 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6781 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6841 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6901 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6961 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7021 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7081 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7141 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7201 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7261 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7321 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7381 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7441 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7501 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7561 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7621 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7681 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7741 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7801 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7861 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7921 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7981 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8041 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8101 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8161 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8221 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8281 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8341 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8401 TTTAACGCGA ATTTT
//