LOCUS       RR205530                7809 bp    DNA     circular  SYN 
DEFINITION  Syne3 (Myc-DDK-tagged ORF) - Rat similar to RIKEN cDNA 4831426I19 (RGD1304563), (10 ug)
ACCESSION   NM_001106762
VERSION     NM_001106762.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3966..4057 
                     /label="Myc-DDK"  
     misc_feature    4087..4672 
                     /label="PolyA signal"  
     misc_feature    4144..4163 
                     /label="Restriction Enzyme"  
     misc_feature    4948..5620 
                     /label="ColE1"  
     misc_feature    6050..6844 
                     /label="Kan/Neo"  
     misc_feature    6879..7181 
                     /label="SV40 ori"  
     misc_feature    7267..7744 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3948..3954 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3948 
                     /label="Syne3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGCAGCAG CCCCAGGAGG ACTTCGACAG GAGTGTGGAG GATGCCCAGG 
     1081 CATGGATGAA GGCAGTACAG GAGCAGCTTC AGGTCAATGA CAACACGAAG GGGCCCCGAG 
     1141 CAGCCCTGGA GGCGAGGCTG CGAGAGACAG AGAAAATCTG TCAGCTGGAG CCTGAGGGAT 
     1201 GCATAAAGGT GGAACTGGTC CTGCGGGCAG CTGAGGCCCT CTTGGCAACT TGCCAGGAGG 
     1261 GCCAGAAACC TGAGATCCTG GCCCGGCTGA GGGATATCAA GTCTCAGTGG GAGGAGACGG 
     1321 TCACCTACAT GACTCACTGC CACAGTCGCA TCGAGTGGGT GTGGCTGCAC TGGAGCGAGT 
     1381 ACCTGCTGGC CCAGGACGAG TTTTACCGAT GGTTCCAGAA GATGGTGGTC ACGCTGGAGC 
     1441 CCCCCGTGGA GCTGCAGCTG GGCCTGAAGG AGAAGCAGTG GCAGCTGAGC CACGCCCAGG 
     1501 TGCTGCTGCA CAATGTGGAC AATCAGGCTG TGCTCCTGGA CAGGCTGCTG GAGGAGGCGG 
     1561 GCTCCCTGTT CAGCAGGATC GGGGACCCCA GCGTGGATGA AGAGGCCCAG AAGAGGATGA 
     1621 AGGCAGAGTA CGATGCCGTG AAGGCCAGAG CTCAGCACAG GGTGGAGCTC CTGGCCCAGG 
     1681 TGGCCCAGGA CCATGAGCAG TACCGGGAGG ACGTGAGCGA GTTCCAGCTG TGGCTGAAGG 
     1741 CGTTGGTGGA GAAGGTGCAC GGCTGCCTGG GGCGGAACTG CAAACTGGCC ACCGAGCCTC 
     1801 GCCTCTCTGT GCTGCAGGAC ATCGCCAAGG ATTTCCCTAG GGGTGAGGAG TCTTTGAACA 
     1861 GATTGGAGGA GCAGGCTGTG GGTGTCATTC GGAACACCTC TCCTTTGGGT GCAGAGAGGA 
     1921 TCTCAGGGGA GCTGGAGGAG ATGCGGATGG TCCTGGAGAA GCTGAGAGTC CTCTGGAAAG 
     1981 AGGAGGAAGG AAGGCTGCGG GGCCTGCTCC AGTCCAAGGG GGCCTGTGAG CAGCAGATCC 
     2041 AGCAGCTGGA GGTGGAACTG GGAGAGCTCA AGAAAGTTCT TCAGAGGCTG GCCCAGCAAG 
     2101 GCTTGGAGCC CACGGTGAAG ACGGCCACAG AGGATGAGCT AGTGGCCCAC TGGAGGCTGT 
     2161 TCTCGGGGAC TCGGGCTGCA CTGGCTTCAG AGGAACCCCG TGTAGACCGG TTACAAACTC 
     2221 AACTAAAGGA ACTTGTCATC TTCCCGGACC TGCAGTCACT CTCTGATAGC GTGGTGGCTG 
     2281 CCATTCAGGA ATACCAAAGT ATGAAGGGTA AGAATGCCAG GCTCCACAAT GCGACCAGGA 
     2341 CGGAGCTATG GCAGCGTTCC CAGCGGTCCC TCAATGACCT GCAGCTATGG AAGGCGCTGG 
     2401 CCCAGAGGCT CCTGGACATC ACCACCAGCC TGCCGGACCT GGCCTCCATT CACACTTTTC 
     2461 TACCCCAGAT TGAGGCAGCC CTAACAGAAA GCTCCCGCCT GAAAGAGCAG CTGTCAATGC 
     2521 TGCAGCTGAA GACAGACCTG CTGGGCAGCA TCTTTGGCCA GGAGAGAGCA GCCACCCTCC 
     2581 TGGAGCAGGT GACAGGCTCT GTGAGGGACA GAGACCTACT GCATAACAGC CTTCTTCAGC 
     2641 GGAAGAGCAA ACTTCAGAGC CTGCTTGTTC AGCACAAGGA CTTTGGGGTA GCTTTTGATC 
     2701 CTCTGCACAG GAAGCTCCTA GACCTCCAAG CCAGGATCCA AGCAGAGAAA GGGCTTCAGC 
     2761 GGGACCTTCC TGGAAAGCAG GTCCAGCTCC TAAGGTTGCA GGGGCTGCAG GAGGAGGGGC 
     2821 TGGATCTGGG GACACAGATC GAGGCTGTGA GATCTCTTGC CCAGGGGAAC TCTAAGTACC 
     2881 AGCACAAGGT AGACCAGATC TCCTCTGACC AGCAAGCCCT GCAGAGGTCC CTGGAGGACC 
     2941 TTGTGGACAG GTGCCAGCAG AATGTACGGG AACACTGTAC CTTCAGTCAC AGGCTGTTGG 
     3001 AGCTCCAGCT GTGGATCACC ATGGCCACAC AGACGTTGGA GTCACACCAA GGGGATATGC 
     3061 GTCTGTGGGA TGCAGAGTCC CAAGAGGCTG GACTCGAGAG GCTGCTGTCT GAAATCCCAG 
     3121 AGAAAGAGGT CCAGCTCTCC CTGCTCCAAG CACTGGGCCA GCTTGTGATG AAGAAGTCTT 
     3181 CTCCAGAAGG GGCAGCCGTG GTCCAGGAGG AGCTGAGGAA GCTGATGGAG TCTTGGCAGG 
     3241 CCTTGCGACT GCTAGAGGAG AGCATGCTGA GTCTCATGAG GAACGAGCAG CTGCAGAGGA 
     3301 CGGAGGTGGA CACGGGGAAG AAGCAGGTTT TCACCAACAA CATCCCAAAG GCTGGCTTTC 
     3361 TCATCAACCC TCAGGATCCC ATTCCCAGGA GACGGCATGG GGTGAACCCA CCAGAAGAAC 
     3421 ACGACCTCCT TGAAGATCAT CCCCAGCTCC TGAGGGAATT TGAACAGTGG CTACAGGCAG 
     3481 AAAACTCCAA GCTACTTAGA ATCACCACAA TGAGAACAGC CACAGCCAAG GACTTGAGGA 
     3541 CCAGAGAGCT TAAGCTGCAG GAGCTGGAGG CTCGGATCCC AGAAGGACAG ATCCTCTTTG 
     3601 AGAACCTGCT TCGTCACAGA CCAGCAAGGG ACCCCTCCAA TGAGCTGGAA GATCTGCGAT 
     3661 ACCGGTGGAT GCTCTACAAG TCCAAGCTCA AGGGCTCCGG CGACCTGCTG ACGGAGAGTT 
     3721 CTCCAGGGGA GCTGACTGCA TTCCAGAAGA GTCGGCAGCA GAAGCGGTGG CGGAGTCCCT 
     3781 GCTCTCTCCT ACAGAAAGCA TGCCGCGTGG CACTGCCGTT GCAGCTTCTG CTCCTGCTCT 
     3841 TTCTGCTGCT GCTGTTCCTG TTGCCAGCGG GCGAGGAGGA GCGCAGCTGC GCCCTGGCCA 
     3901 ACAACTTCGC TCGCTCTTTT GCGCTCATGC TGCGGTACAA CGGCCCTCCG CCCACCACGC 
     3961 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4021 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4081 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4141 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4201 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4261 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4321 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4381 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4441 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4501 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4561 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4621 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4681 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4741 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4801 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4861 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4921 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4981 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5041 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5101 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5161 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5221 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5281 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5341 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5401 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5461 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5521 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5581 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5641 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5701 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5761 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5821 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5881 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5941 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6001 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6061 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6121 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6181 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6241 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6301 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6361 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6421 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6481 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6541 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6601 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6661 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6721 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6781 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6841 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6901 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6961 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7021 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7081 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7141 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7201 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7261 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7321 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7381 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7441 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7501 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7561 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7621 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7681 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7741 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7801 GCGAATTTT
//