LOCUS       RR205487                8133 bp    DNA     circular  SYN 
DEFINITION  Usp8 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 8 (Usp8), (10 ug)
ACCESSION   NM_001106502
VERSION     NM_001106502.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4288..4379 
                     /label="Myc-DDK"  
     misc_feature    4409..4994 
                     /label="PolyA signal"  
     misc_feature    4466..4485 
                     /label="Restriction Enzyme"  
     misc_feature    5270..5942 
                     /label="ColE1"  
     misc_feature    6372..7166 
                     /label="Kan/Neo"  
     misc_feature    7201..7503 
                     /label="SV40 ori"  
     misc_feature    7589..8066 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4263..4270 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4263 
                     /label="Usp8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTGCTGTA GCTTCAGTTC CTAAAGAACT CTACCTCAGT TCTTCACTCA 
     1081 AAGACCTCAA TAAGAAGACA GAAGTTAAAC CTGAGAAAAC CAGCACCAAG AATTATATAC 
     1141 ACAGCGCTCA GAAGATTTTC AAGGCAGCAG AAGAATGCAG ACTAGATCGT GATGAGGAAA 
     1201 GGGCCTATGT GCTTTATATG AAATATGTGG CGGTTTATAA TCTTATCAAA AAGAGACCTG 
     1261 ATTTCAAGCA ACAGCAGGAC TATTACCTTT CAATACTTGG ACCTGCAAAC ATCAAAAAGG 
     1321 CTATTGAAGA AGCTGAAAGA CTCTCTGAGA GCCTCAAACT GAGATACGAA GAGGCTGAAG 
     1381 TTCGGAAACA GCTTGAAGAA AAGGACAGAC GGGAGGAAGA ACAGCTGCAG CAACAGAAAA 
     1441 GGCAGGAGAT GGGAAGAGAG GATAGTGGTG CGGCGGCCAA ACGCTCCGTG GAAAATTTAC 
     1501 TGGATTCCAA AACCAAAATC CAAAGGGTCA ATGGAGAGAA GAGCGAAGGA GGTGCAGCAG 
     1561 CAGAGAGAGG AGCAGTCACA GCAAAGGAGC TATATACAAT GATGATGGAT AAAAACACAA 
     1621 GCCTGATTAT AATGGATGCT CGGAAAATAC AGGATTATCA GCATTCCTGT ATCTTGGGCT 
     1681 CTCTCAGTGT TCCTGAAGAA GCTATCAGTC CAGGAGTCAC TGCCAATTGG ATTGAAGCAA 
     1741 AGCTTTCAGA TGATTCTAAA GACACGTGGA AAAAAAGGGG GAGTGTGGAC TATGTGGTCC 
     1801 TGCTGGACTG GTTTAGTTCA GCGAAAGATT TGCTGCTCGG GACCACGCTA CGGAGTCTGA 
     1861 AAGATGCTCT TTTCAAGTGG GAAAGTAAAG CTGTCCTGCA CCATGAGCCC TTGGTGCTGG 
     1921 AGGGCGGCTA TGAGAACTGG CTCCTGTGCT ATCCACAGTT CACAACAAAT GCTAAGGTTA 
     1981 CTCCACCCCC TCGGAGCAGG CCTGAAGAGG TGTCTGTCTC ATTGGATTTT ACTTATCCCT 
     2041 CACTGGAAGA ACCAGTTCCT TCCAAACTTC CTGCCCAGAT GCCACCTCCT CCTTTAGAAG 
     2101 CAAATGAGAA GGCACAGTTG GTAACTGATC AAGATGGTAA ACCGAGACCG TTAGTCCAGT 
     2161 CGGCTCTAGC CGGACCCAGT GTGGCTCCCA AAGCTGAAGC TTCACCCATA ATTCAGCCAG 
     2221 TGCCTGCTAC AAAGAATGTT CCACAGGTTG ATCGTACAAA AAAACCAGCA GTTAAGTTGC 
     2281 CTGAAGATCA TAGAATGAAA TCTGAAAGTA CAGATCAGAG TGGAAGAGTT CTTTCTGACC 
     2341 GATCCACCAA GCCAGTATTT ACCTCTCCAG CCACCATGCT AACAGATGAA GAAAAGGCTC 
     2401 GCATTCATGA AGAAACAGCC CTTCTTATGG AAAAGAATAG ACAGGAGAAG GAACTGTGGG 
     2461 AAAGGCAGCA GAAGGAACAG AAAGAGAAGC TGAGAAGGGA GGAACAAGAG CGCAAAGCTG 
     2521 GAAAGACCCA GGATGCAGAA GAACGGGACT TCACTGAGAA TCAGCACAAA GCAAAGGATG 
     2581 GACAAGAGAA GAGAGACAGC AAACAGACCA AGGCAGAAGA CAGAGAGCCC CCAGCAGATG 
     2641 GGGCCCAGGA CGCCACAGGA ACGCAAAGAC AAAGTAAGAG TGAACACGAT GCTTCCGATG 
     2701 CTAAGGTATC TGTGGAAGGT AAAAGGTGTC CCATGTCAGA AGTGCAGAAG AGGCCAGCAG 
     2761 ATGTGTCGTC TGCATCCTCC ATGTCGGGAG AGCTGAGTGC AGGCAAGGCT CAGCGAGAAC 
     2821 CTTTGACAAG AGCAAGAAGT GAAGAAATGG GGAGAATTGT GCCAGGACTG CCTTTAGGCT 
     2881 GGGCCAAGTT TCTTGATCCA ATCACTGGGA CTTTTCGTTA CTACCATTCA CCCACAAACA 
     2941 CGGTTCACAT GTATCCGCCT GAGATGGCTC CTTCGTCTGT GCCTCCTTCC ACCCCTCCCA 
     3001 CTCACAAAGT CAAGCCCCAG ATCCCTGCTG AGCGGGACAG GGAGCCATCC AAACTGAAGC 
     3061 GCTCCTACTC CTCACCAGAC ATCACTCAGG CCCTGCAGGA GGAGGAGAAG AGGAGGCCAG 
     3121 CAGTGACCCC GACAGTCAAC CGGGAGAACA AGCCGCCGTG TTACCCTAAA GCTGAGATCT 
     3181 CGAGGCTTTC TGCTTCTCAG ATTCGGAACC TCAATCCCGT TTTTGGAGGA TCTGGACCAG 
     3241 CTCTTACTGG ACTTCGTAAT TTGGGAAATA CTTGTTACAT GAACTCAATA TTACAGTGCC 
     3301 TGTGCAATGC TCCACATTTG GCTGATTATT TCAACCGAAA CTGCTACCAG GACGATATCA 
     3361 ACAGGTCAAA TTTGTTGGGG CATAAAGGTG AAGTGGCAGA AGAATTTGGT ATAATCATGA 
     3421 AGGCACTGTG GACAGGACAG TATAGATACA TCAGTCCAAA AGACTTTAAA GTCACCATTG 
     3481 GTAAGATTAA TGACCAGTTT GCAGGATCCA GCCAGCAAGA TTCACAAGAG CTGCTGCTTT 
     3541 TCCTCATGGA TGGCCTCCAC GAGGATCTAA ATAAGGCTGA CAATCGGAAG AGGCATAAGG 
     3601 AAGAGAACAA TGACCACCTC GATGACTTTA AAGCGGCAGA ACATGCCTGG CAGAAGCACA 
     3661 AGCAGCTCAA TGAGTCCATT ATTGTCGCAC TCTTTCAGGG TCAGTTCAAA TCCACAGTGC 
     3721 AGTGCCTCAC CTGTCACAAG AAGTCTCGGA CATTCGAGGC TTTCATGTAT TTGTCTTTGC 
     3781 CACTAGCATC CACAAGTAAA TGTACTTTAC AGGACTGCCT TAGATTATTT TCCAAAGAAG 
     3841 AAAAGCTTAC AGATAACAAC AGATTTTACT GCAGCCATTG CCGAGCTCGG CGGGATTCTC 
     3901 TAAAGAAAAT AGAAATCTGG AAATTGCCTC CTGTGCTGTT AGTACACCTG AAACGATTCT 
     3961 CCTATGACGG CAGGTGGAAG CAGAAACTGC AAACGTCCGT GGATTTCCCA TTAGAAAATC 
     4021 TTGACCTGTC ACAGTATGTT ATTGGCCCAA AAAGCAGTTT GAAGAAATAT AACTTGTTTT 
     4081 CTGTTTCAAA CCACTACGGC GGGCTCGATG GAGGCCACTA CACGGCCTAT TGTAAAAACG 
     4141 CGGCGAGGCA GCGCTGGTTT AAGTTTGATG ACCACGAGGT TTCGGACATC TCCGTGTCTT 
     4201 CTGTGAGGTC GTCAGCAGCT TATATCCTGT TTTACACCTC CCTGGGGCCA CGCGTGACTG 
     4261 AGGCAGCCAC AAGCGGACCG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4321 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4381 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4441 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4501 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4561 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4621 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4681 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4741 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4801 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4861 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4921 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4981 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5041 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5101 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5161 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5221 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5281 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5341 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5401 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5461 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5521 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5581 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5641 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5701 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5761 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5821 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5881 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5941 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6001 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6061 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6121 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6181 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6241 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6301 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6361 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6421 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6481 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6541 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6601 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6661 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6721 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6781 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6841 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6901 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6961 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7021 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7081 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7141 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7201 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7261 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7321 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7381 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7441 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7501 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7561 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7621 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7681 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7741 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7801 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7861 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7921 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7981 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8041 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8101 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//