LOCUS       RR205470                8502 bp    DNA     circular  SYN 
DEFINITION  Rbm20 (Myc-DDK-tagged ORF) - Rat RNA binding motif protein 20 (Rbm20), (10 ug)
ACCESSION   NM_001107611
VERSION     NM_001107611.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4659..4750 
                     /label="Myc-DDK"  
     misc_feature    4780..5365 
                     /label="PolyA signal"  
     misc_feature    4837..4856 
                     /label="Restriction Enzyme"  
     misc_feature    5641..6313 
                     /label="ColE1"  
     misc_feature    6743..7537 
                     /label="Kan/Neo"  
     misc_feature    7572..7874 
                     /label="SV40 ori"  
     misc_feature    7960..8437 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4641..4647 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4641 
                     /label="Rbm20"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGCTGGCT GCAGCCATGA GCCAAGACGC GGATCCCAGC GGTCCGGAGC 
     1081 AACCCGACAG AGATGCCTGC ATTGTGCCTG GTGTTCAAGG GCCCCCTGCG CCCCAAGGCC 
     1141 AGCAAGGGAT GCAGCCCCTG CCGCCACCGC TACCGCCACC GCCTCAGCCT CAATCCAGCC 
     1201 TGCCCCAGAT CATCCAAAAT GCTGCCAAGC TCCTGGACAA GAACCCTTTC TCCGTCAGTA 
     1261 GCCAGAACCC TCTGCTCACT TCGCCAGCCA GCGTCCAGCT GGCCCAGATA CAGGCCCAGC 
     1321 TCACCCTCCA TCGGCTGAAG ATGGCACAGA CCGCAGTCAC CAACAACACT GCAGCCGCCA 
     1381 CTGTCCTCAA CCAAGTCCTC TCCAAAGTGG CCATGTCCCA GCCTCTCTTC AACCAGCTTC 
     1441 GCCATCCGTC TGTGCTTGGC ACCACACATG GCCCTACTGG GGTCTCCCAG CATGCTGCCA 
     1501 CCGTTCCCAG TGCTCACTTT CCCTCAACTG CAATCGCCTT TTCGCCCCCA AGCCAGGCAG 
     1561 GAGGCCCGGG GCCTTCTGTG AGCCTCCCCA GCCAGCCCCC CAATGCTATG GTAGTGCATA 
     1621 CCTTCAGTGG GGTGGTGCCT CAGACCCCTG CCCAGCCAGC AGTCATCCTA AGCATTGGGA 
     1681 AGGCGGGGCC GACACCTGCT ACTACAGGGT TCTATGACTA TGGCAAAGCC AACCCTGGCC 
     1741 AGGCCTATGG TTCTGAAACG GAGGGCCAGC CGGGCTTCTT GCCAGCCTCG GCGTCAGCCG 
     1801 CAGCATCAGG CGGTGTGACC TATGAAGGAC ACTATAGCCA CACAGGGCAG GATGGCCAAG 
     1861 CCACCTTTTC TAAAGACTTC TATGGACCCA GTGCCCAAGG GTCACATGCA GCAGGTGGGT 
     1921 TCCCAGCTGA TCAGGCTGGG AGCATGAAAG GAGACGTCGG TGGGTTGTTG CAAGGTACCA 
     1981 ACAGCCAGTG GGAGAGGCCC TCTGGGTTCT CTGGCCAGAA CAAGGCCGAT ATTACAGCCG 
     2041 GGCCCGGTTT GTGGGCTCCA CCTGCCAGTC AGCCTTATGA ACTATATGAT CCTGAGGAGC 
     2101 CTACCTCAGA CAGGGCCCCT CCTGCCTTTG GATCTCGACT TAACAACAGC AAGCAGGGAT 
     2161 TCAACTGCTC CTGCCGGCGG ACAAAGGAGG GGCAGGCCAT GCTGTCCGTG AGGCCCCTGC 
     2221 AGGGTCATCA ACTGAATGAC TTCCGAGGCT TGGCTCCACT CCACCTTCCA CATATCTGCA 
     2281 GCATCTGTGA CAAGAAGGTG TTTGACCTGA AGGACTGGGA GCTACATGTG AAGGGGAAGT 
     2341 TGCATGCCCA GAAATGCCTG CTCTTCTCAG AAAGTGCTGG CCTCCGGAGT ATATGTGCTA 
     2401 CAGGAGAAGG GACGCTGTCT GCCTCTGCAA ACAGCACAGC TGTTTATAAC CCCACTGGAA 
     2461 ATGAGGATTA TACCTCAACT CTTGGAACAT CATATGCAGC CATTCCAACA AGGGCCTTTG 
     2521 CTCAATCAAA CCCCATGTTT CCTTCGGCTT CCTCTGGGAC AAATTTTGCA CAGAGGAAAG 
     2581 GTGCTGGACG AGTTGTGCAC ATCTGCAATC TCCCAGAGGG CAGCTGCACG GAGAATGACG 
     2641 TCATCAACCT GGGGCTGCCC TTTGGCAAGG TCACTAATTA CATCCTTATG AAGTCAACTA 
     2701 ATCAGGCTTT CTTGGAGATG GCTTACACAG AAGCTGCTCA AGCTATGGTC CAGTACTACC 
     2761 AAGAAAAGCC CGCGCTTATC AATGGCGAGA AGTTACTCAT TCGGATGTCC ACGAGATACA 
     2821 AGGAATTGCA GCTGAAGAAA CCTGGGAAAA ATGTGGCTGC TATCATCCAG GACATCCACT 
     2881 CCCAAAGGGA GAGGTGCATG CTCCGGGAAG CTGACAGATA TGGTCCAGAG CGACCACGTT 
     2941 CTCGAAGTCC AATGAGCCGA TCGCTCTCCC CGAGATCCCA CAGTCCTCCT GGCCCCTCTC 
     3001 GGGCTGATTG GGGCAATGGC CGTGACTCCT ACGCATGGAG GGACGAGGAT CGGGAGACGG 
     3061 TGCCCAGGAG GGAGAACGGG GAAGACAAGA GGGACAGGTT GGATGTTTGG GCACATGACC 
     3121 GGAAACACTA TCCCAGGCAG CTGGACAAAG CCGAGTTGGA TGAGCGACTC GAAGGAGGGA 
     3181 GGGGCTACCG GGAAAAGTAC TTGAAGTCAG GGTCCCCCGG CCCACTCCAT TCTGCGTCTG 
     3241 GCTACAAAGG CCGGGAAGAT GGCTACCATC GAAAAGAGAC TAAAGCTAAG TTGGACAAAC 
     3301 ACCCAAAGCA GCAGCAGCAG GATGTGCCAG GAAGATCCAG GAGGAAAGAA GAGGCGCGAC 
     3361 TACGGGAGCC CAGACACCCT CACCCAGAGG ACTCTGGCAA GGAAGAGGAT CTGGAGCCCA 
     3421 AGGTCACTCG GGCCCCCGAG GGTACCAAGT CCAAGCAAAG TGAGAAAAGT AAAACCAAGA 
     3481 GAGCCGACAG AGACCAAGAA GGAGCTGATG ACAAAAAAGA AGGCCGAGGG GCAGAGAATG 
     3541 AGGCTGGGAC TGAGGAACAG GAAGGCATGG AAGAGAGCCC CGCATCGGTG GGCACACAGC 
     3601 AGGAAGGGAC GGAGTCCTCC GATCCAGAAA ACACAAGGAC AAAGAAGGGA CAAGACTGTG 
     3661 ACAGTGGAAG TGAGCCTGAG GGGGACAACT GGTACCCCAC CAACATGGAG GAGCTGGTCA 
     3721 CAGTGGACGA AGTAGGGGAG GAAGACTTCA TCATGGAGCC AGACATACCG GAGCTGGAAG 
     3781 AAATTGTACC CATCGACCAG AAAGACAAAA TCCTCCCCGA AATATGTCCC TGTGTAACAG 
     3841 CCACCTTAGG TTTGGACTTG GCCAAAGACT TCACCAAGCA GGGAGAGACC CTAGGGAACG 
     3901 GAGACGCAGA ACTCAGCCCG AAGCTGCCCG GACAAGTGCC GTCTACTTCC ACAAGCTGTC 
     3961 CCAATGACAC GGACATGGAG ATGGCTGGCC TAAATCTGGA TGCTGAGCGG AAGCCAGCTG 
     4021 AAAGCGAGAC AGGCCTCTCA CCGGAGGTCT CAAATTGCTA CGAGAAGGAG GCGAGAGGAG 
     4081 CGGAGGGCTC AGATGTGCGT CTGGCCCCTG CAGCCCAGCG AATGTCCTCC CCTCAGCCAG 
     4141 CAGATGAGAG GGCCCGGCAG TCCAGCCCTT TTCTCGATGA CTGCAAGGCC AGGGGGTCCC 
     4201 CAGAAGATGG GCCTCATGAA GTCAGCCCCC TGGAAGAAAA AGCCAGCCCC ACTACTGAAA 
     4261 GCGACCTCCA AAGCCAGGCT TGCCAAGAAA ACTCCAGGTA CACGGAGACG AGATCTCTGA 
     4321 ACTCGAGATC ACCAGAATTC ACCGAAGCGG AGCTGAAAGA GCCCCTTTCT TTGCCTTCCT 
     4381 GGGAACCAGA GGTGTTCAGT GAACTTAGCA TTCCTCTAGG AGTGGAGTTC GTGGTTCCCA 
     4441 GGACTGGCTT TTACTGCAAG CTGTGTGGCC TGTTCTACAC AAGCGAGGAG GCGGCCAAAG 
     4501 TGAGCCACTG CCGCAGCACT GTACACTACA GGAACTTACA GAAGTACCTG TCCCAGCTGG 
     4561 CAGAGGAGGG ACTGAAGGAG ACAGAGGGGG TAGACAGCCC AAGCCCCGAG CGCAGCGGGA 
     4621 TTGGTCCACA CTTGGAAAGG AAGAAGCTAA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4681 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4741 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4801 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4861 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4921 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4981 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5041 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5101 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5161 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5221 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5281 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5341 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5401 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5461 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5521 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5581 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5641 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5701 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5761 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5821 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5881 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5941 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6001 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6061 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6121 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6181 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6241 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6301 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6361 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6421 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6481 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6541 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6601 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6661 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6721 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6781 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6841 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6901 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6961 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7021 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7081 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7141 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7201 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7261 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7321 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7381 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7441 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7501 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7561 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7621 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7681 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7741 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7801 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7861 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7921 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7981 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8041 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8101 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8161 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8221 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8281 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8341 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8401 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8461 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//