LOCUS       RR205465                7920 bp    DNA     circular  SYN 
DEFINITION  Gart (Myc-DDK-tagged ORF) - Rat phosphoribosylglycinamide formyltransferase (Gart), (10 ug)
ACCESSION   NM_001011899
VERSION     NM_001011899.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4075..4166 
                     /label="Myc-DDK"  
     misc_feature    4196..4781 
                     /label="PolyA signal"  
     misc_feature    4253..4272 
                     /label="Restriction Enzyme"  
     misc_feature    5057..5729 
                     /label="ColE1"  
     misc_feature    6159..6953 
                     /label="Kan/Neo"  
     misc_feature    6988..7290 
                     /label="SV40 ori"  
     misc_feature    7376..7853 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4050..4057 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4050 
                     /label="Gart"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGCCCGA GTTCTTGTAA TCGGTAGTGG AGGAAGGGAA CACACCCTGG 
     1081 CCTGGAAACT CGCACAATCC CCTCATGTCA AACAAGTGCT GGTTGCCCCA GGAAATGCAG 
     1141 GCACAGCTTC TGCTGGGAAG ATCTCCAATG CTGCTGTCTC CATCAATGAT CACACTGCTC 
     1201 TCGCTCGGTT CTGCAAAGAT GAAAAGATTG AACTCGTAGT TGTTGGACCA GAGGCCCCTC 
     1261 TGGCTGCAGG GATTGTTGGG GACCTGACTT CTGCAGGAGT GCGATGCTTT GGCCCCACGG 
     1321 CACAAGCAGC TCTACTGGAG TCCAGTAAAA AGTTTGCCAA AGAGTTTATG GACCGACATG 
     1381 GCGTCCCAAC TGCACAGTGG CGGGCATTCA CCAGCCCTGA AGACGCCTGC AGCTTTATTA 
     1441 TGAGCGCGGA CTTTCCTGCT TTGGTTGTGA AGGCCAGTGG CCTTGCAGCA GGGAAGGGGG 
     1501 TGATTGTGGC GAAGAGCAAA GCTGAAGCCT GCGAAGCTGT TCAGGAGATC ATGCAAGAAA 
     1561 AGTCATTTGG AGCAGCCGGT GAAACTGTTG TCGTGGAGGA GCTTCTGGAA GGGGAAGAAG 
     1621 TGTCTTGCCT GTGCTTCACT GATGGGAAGA CAGTGGCCCC AATGCCCCCA GCACAAGACC 
     1681 ATAAGCGATT GCTGGATGGA GATCGGGGAC CCAACACTGG AGGAATGGGA GCCTACTGCC 
     1741 CTGCACCTCA GGTTCCTAAG GATTTGTTAG TAAAAATTAA AAACACAATT CTTCAGAGAA 
     1801 CAGTAGATGG CATGCAGCAG GAGGGGGTGC CGTACACAGG AATTCTTTAT GCTGGTATAA 
     1861 TGCTGACCAA AGATGGCCCA AAAGTTTTGG AGTTTAATTG CCGTTTTGGT GATCCAGAAT 
     1921 GCCAGGTCAT CCTCCCACTT CTTAAAAGTG ACCTTTATGA AGTGATTCAG TCCACCTTTG 
     1981 ATGGCCTGCT GAGCGAGTCT CCGCCTGTTT GGCTAGAAAA CCACAGTGCC GTAACTGTTG 
     2041 TCATGGCGAG TGGAGGGTAT CCTGGAGCCT ACACCAAGGG TGTGGAGATT ACAGGGTTTC 
     2101 CTGAGGCCCA AGCTTTAGGA CTGCAGGTCT TTCATGCAGG CACTGCTCTT AAAGATGCCA 
     2161 AAGTGGTGAC CAGCGGGGGC AGAGTCCTCA CAGTGACAGC TGTCCGGGAA AATCTCATGT 
     2221 CAGCCCTTGA TGAGGCCAGG AAGGGACTCG CTGCTCTAAA GTTTGAGGGA GCAGTTTACC 
     2281 GAAAGGACAT TGGCTTCCGT GCTGTGGCCT TCCTCCAGCG GCCCAGGGGC CTGACCTACA 
     2341 AGGACTCTGG AGTAGACATT GCAGCTGGAA ATATGCTGGT TAAGAAAATT CAGCCTTTAG 
     2401 CCAAAGCTAC CTCCAGACCA GGCTGCAGTG TTGACCTTGG GGGCTTTGCT GGTCTTTTTG 
     2461 ATTTGAAAGC AGCTGGTTTC AAAGATCCTC TTCTGGCCTC TGGAACAGAC GGTGTTGGAA 
     2521 CTAAACTAAA GATTGCCCAG CTGTGCAATA AGCATGACTC CATCGGTCAG GATCTGGTTG 
     2581 CGATGTGCGT GAACGACATC CTCGCACAAG GAGCAGAGCC CCTCTTCTTC CTTGACTACT 
     2641 TTTCCTGTGG AAAACTTGAC CTTAGTACAA CTGAGGCTGT GGTTGCTGGA ATTGCTGCAG 
     2701 CTTGCCGACA GGCTGGGTGT GCTCTCCTAG GTGGGGAGAC AGCAGAGATG CCCGACATGT 
     2761 ACCCCCCTGG AGAGTATGAT CTTGCCGGAT TTGCTGTTGG TGCTATGGAG AGACACCAGA 
     2821 AGCTCCCTCA GCTAGAAAGG ATCGCCGAAG GAGATGCCGT TATTGGAGTG GCTTCATCTG 
     2881 GTCTTCACAG CAATGGCTTT AGCCTTGTGA GGAAAATTGT GGAGAGGTCT TCACTCCAGT 
     2941 ACTCCTCTCC AGCACCGGGT GGTTGTGGAG ATCAGACTTT AGGGGACTTA CTTCTAACTC 
     3001 CAACCAGGAT CTACAGCCAT TCACTGCTGC CTATCATACG TTCAGGACGT GTCAAAGCTT 
     3061 TTGCTCATAT TACTGGAGGT GGATTGCTGG AAAACATCCC CAGAGTCCTC CCTCGGAAGC 
     3121 TTGGAGTAGA TTTAGATGCC TATACCTGGA GGGTCCCCAA GGTCTTCTCA TGGCTACAGC 
     3181 AAGAAGGACA GCTCTCTGAA GAAGAAATGG CCAGGACATT CAACTGTGGG GTTGGAGCTG 
     3241 CCCTGGTGGT ATCAAAGGAC CAAACAGAGC AGATTCTCCA TGACATTCGG CAGCACCAGG 
     3301 AAGACGCCTG GGTGATAGGC AGTGTCGTTG AGTGTCCCGA AGATTCCCCT CGTGTCAGAG 
     3361 TTAAGAATCT GATTGAAACC ATGCAAACAA AGGGGTCAGT GGTGACAAAC GGCTTCCTGA 
     3421 GAAGTAATTT CCCTAGCCAG CAAAAGAAAG CAAGAGTGGC TGTCCTAATA TCTGGAACAG 
     3481 GGTCGAACCT CCAGGCACTC ATAGACAGCA CTCGGGATCC AAAAAGCTCC TCACACATCG 
     3541 TTCTTGTCAT CTCCAACAAA GCTGCAGTTG CTGGCTTAGA CCGAGCAGAG CGAGCTGGCA 
     3601 TTCCCACCAG GGTGATTAAT CATAAGTTAT ATAAAAGTCG TGTCGAGTTT GACAACGCAG 
     3661 TTGACCATGT CCTGGAAGAG TTCTCAGTAG ACATAGTCTG TCTCGCAGGA TTCATGAGGA 
     3721 TCCTCTCTGG CCCGTTTGTC AGAAAATGGG ATGGGAAAAT GCTCAACATC CACCCGTCCT 
     3781 TGCTCCCTTC TTTTAAGGGT TCAAATGCTC ACGAGCAGGT TCTGGAGGCT GGAGTCACCA 
     3841 TTACTGGGTG CACCGTACAC TTTGTAGCTG AAGACGTAGA TGCCGGGCAA ATCATCCTAC 
     3901 AGGAAGCTGT CCCTGTGCAG AGGGACGACA CTGTTGCAAC ACTGTCTGAA CGCGTCAAGG 
     3961 CAGCAGAACA TAAGATATTT CCTGCAGCCC TCCAACTAGT GGCCAGTGGG GCTGTGCAGC 
     4021 TGGGAGAAGA CGGCAAGATC CACTGGGCCA GAGAGCAGAG CGGACCGACG CGTACGCGGC 
     4081 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4141 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4201 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4261 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4321 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4381 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4441 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4501 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4561 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4621 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4681 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4741 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4801 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4861 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4921 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4981 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5041 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5101 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5161 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5221 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5281 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5341 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5401 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5461 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5521 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5581 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5641 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5701 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5761 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5821 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5881 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5941 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6001 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6061 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6121 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6181 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6241 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6301 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6361 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6421 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6481 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6541 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6601 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6661 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6721 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6781 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6841 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6901 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6961 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7021 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7081 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7141 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7201 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7261 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7321 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7381 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7441 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7501 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7561 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7621 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7681 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7741 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7801 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7861 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//