LOCUS       RR205417                9339 bp    DNA     circular  SYN 
DEFINITION  Eprs (Myc-DDK-tagged ORF) - Rat glutamyl-prolyl-tRNA synthetase (Eprs), (10 ug)
ACCESSION   NM_001024238
VERSION     NM_001024238.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5496..5587 
                     /label="Myc-DDK"  
     misc_feature    5617..6202 
                     /label="PolyA signal"  
     misc_feature    5674..5693 
                     /label="Restriction Enzyme"  
     misc_feature    6478..7150 
                     /label="ColE1"  
     misc_feature    7580..8374 
                     /label="Kan/Neo"  
     misc_feature    8409..8711 
                     /label="SV40 ori"  
     misc_feature    8797..9274 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5478..5484 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5478 
                     /label="Eprs"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGCTG TGCCTTACGG TGAACGCCGG AAACCCTCCA CTGGGTTGTC 
     1081 GAGTTAAAGT GAACAAGAGA AATAAATCCT TCATGTTGTC ATTTTTAGAA GCTCTGCTGG 
     1141 CAGTGGAGCA TGTGAAAGGT GATGTCAGCA TTTCTGTGGA AGAAGGGAAG GAGAATCTTC 
     1201 TTCGGGTTTC TGAGAGTGTG GTGTTCACTG ACACAAATTC AATCCTGCGC TACTTGGCTA 
     1261 GAGTTGCAGC TACATCTGGG CTGTACGGTT CTAACCTGAT GGAGCACACG GAGATTGATC 
     1321 ATTGGTTGGA GTTCAGCGCT ACAAAGTTGT CTTCGTGTGA TGTGCTGACT TCGGCAATCA 
     1381 AGGAGCTTAA CCATTGCCTG TCTCTGAGAA CATACCTGGT TGGGAACTCT TTGACTTTAG 
     1441 CAGATTTATG TGTTTGGGCC ACCCTAAAAG GAAATGCTGC ATGTCAGGAG CACTTGAGAC 
     1501 AGAGCAAAAC CCTAGTCCAC GTTAACCGCT GGTATGGCTT TCTTGAAGCC CAGCAGGCCT 
     1561 TCCGTTCAGT TGGTACCAAG TGGGATGCTT CAGCAAACAA AGCTACAGGG GCACCTGACA 
     1621 GAAAACAAGA TATTGGGAAA TTCGTCGAGC TTCCGGGTGC AGAGATGGGA AAGGTGACAG 
     1681 TCAGATTCCC CCCAGAGGCT AGTGGTTACT TACACATTGG GCATGCAAAG GCTGCTCTTC 
     1741 TGAACCAGCA CTACCAGGTT AACTTTAAAG GCAAACTGAT CATGAGATTT GATGACACAA 
     1801 ACCCAGAAAA GGAAAAGGAA GATTTTGAGA AGGTCATCTT GGAAGATGTT GCAATGCTGC 
     1861 ACATTAAACC AGATCAGTTT ACTTACACTT CAGATCATTT TGAAACTATA ATGAAGTATG 
     1921 CAGAGAAATT AATTGAAGAA GGGAAGGCCT ATGTGGACGA CACTCCCGCA GACGAGATGA 
     1981 AGGCGGAGCC TGTTGAGAAG AATCTACAAA TGTGGGAAGA AATGAAAAGA GGAAGCCAGT 
     2041 TTGGTCAGTC CTGTTGCCTG CGAGCAAAAA TTGACATGAG TAGTAACAAT GGGTGTATGA 
     2101 GGGACCCAAC CCTTTACCGC TGCAAAATCC AGCCCCACCC AAGAACTGGG AATAAATACA 
     2161 ATGTTTACCC AACGTATGAC TTTGCCTGTC CTATAGTGGA CAGCATTGAA GGTGTTACTC 
     2221 ATGCCTTGAG GACGACAGAG TACCACGACC GAGATGAGCA GTTTTACTGG ATTATTGAAG 
     2281 CCTTAGGCAT AAGGAAACCA TACATCTGGG AATACAGTCG CTTAAATCTC AACAACACAG 
     2341 TGCTGTCCAA GAGAAAGCTT ACGTGGTTTG TCAATGAAGG ACTGGGCTCC TCAAGGTCTG 
     2401 TTGTAAACAT GGAATGGGAC AAAATCTGGG CGTTTAACAA AAAGGTTATT GACCCTGTGG 
     2461 CTCCAAGGTA CGTTGCATTG CTGAAGAAGG AGGTGATCCC AGTGAACATC CTGGACGCGC 
     2521 AGGAGGAGAT GAAGGAAGTA GCCAGACATC CCAAGAATCC TGATGTTGGT TTGAAGCCTG 
     2581 TGTGGTATAG TCCCAAGGTT TTCATAGAAG GAGCTGATGC AGAGACTTTC TCAGAGGGTG 
     2641 AAATGGTCAC ATTTATAAAC TGGGGCAACA TCAACATTAC TAAAATACAC AAAAATCCAG 
     2701 ATGGAAAAAT TACATCTCTG GATGCAAAAT TGAATTTGGA AAACAAAGAC TACAAGAAAA 
     2761 CAACTAAGAT CACTTGGCTT GCGGAGACCA CACAGGCTCT CCCTGTCCCG GCAGTCTGTG 
     2821 TCACTTATGA GCACTTGATT ACAAAGCCAG TGCTAGGAAA AGACGAGGAC TTCAAGCAGT 
     2881 ACGTCAACAA AGACAGTAAG CATGAAGAAC TAATGCTAGG AGATCCCTGC CTTAAGGATT 
     2941 TGAAGAAAGG AGACATCATA CAATTGCAGA GGCGCGGATT CTTCATATGC GACCAGCCGT 
     3001 ACGAACCTGT CAGCCCATAT AGTTGCAAAG AAGCCCCCTG CATCTTGATA TATATACCCG 
     3061 ATGGGCACAC CAAGGAGATG CCAACATCTG GATCAAAGGA AAAGACTAAA GTGGAAATCT 
     3121 CAAAAAAGGA GACCGGCTCT GCTCCTAAGG AGAGACCCGC AGCTGCTGTG AGTGCTCCCT 
     3181 GTGCTGCAGC TGAGGACGCT GCCATCCTCT ACAGCAGAGT GGCGGTCCAG GGTGATGCGG 
     3241 TTCGCGACCT GAAAGCGAAG AAAGCACCAA AGGAGGATAT TGACGCAGCT GTGAAACAGC 
     3301 TTTTGACTTT GAAGGCTGAG TATAAAGAGA AAACTGGGCA GGAGTATAGA CCTGGGAACC 
     3361 CCCCTACTGC AGCAGTGCAG ACTGTTTCTA CAAAGTCCCC ATCCAACACC GGGGAATACA 
     3421 CATCTTTATA CAATAAAGTT GCTGCCCAAG GGGAGCTGGT CCGGAAGCTG AAAGCTGAAA 
     3481 AGGCTCCTAA GGCTAAAATA AATGATGCTG TAGAATGCTT ACTGTCCCTG AAAGCTGAGT 
     3541 ATAAAGAAAA AACTGGGAAG GAGTATGTAC CTGGCCAACT CCCAGCATCT CAGAACTCAC 
     3601 ATTCAAGTCC TGTCAGCAAT GCCAAGCCTG TTGGATCAGA GACACCGGAA GCCAAACTGC 
     3661 TGTTTGACAG AGTGGCTTGT CAAGGAGAAG TAGTTCGGAA ACTTAAAGCT GAGAAGGCCT 
     3721 CTAAGGATCA AGTGGATTCA GCCGTACAAG AACTTCTTCA GCTGAAGGCA CAGTACAAGT 
     3781 CACTGACAGG AATTGAGTAT AAGCCTGTGT CTGCTACTGG GGCAGAAGAC AAAGATAAGA 
     3841 AGAAGAAAGA AAAAGAAAAT AAGTCTGAAA AGCAGAATAA GCCTCAGAAA CAAAATGATG 
     3901 GCCAAGGGAA AGACTCTTCT AAAAGCCAAG GCAGCGGGCT GTCGTCCGGT GGAGCAGGAG 
     3961 AAGGGCAAGG GCCCAAGAAA CAGACCAGAT TGGGTCTTGA AGCAAAAAAA GAAGAAAATC 
     4021 TTGCCGAATG GTATTCCCAA GTCATCACGA AGTCAGAGAT GATCGAATAC TACGATGTGA 
     4081 GTGGCTGCTA TATTCTCCGA CCCTGGTCGT ACTCCATCTG GGAATCCATT AAGGACTTCT 
     4141 TTGACGCGGA GATCAAGAAG CTCGGCGTTG AGAACTGCTA CTTCCCCATA TTCGTGTCCC 
     4201 AGGGCGCACT AGAGAAGGAG AAGAACCACA TTGCTGACTT TGCTCCCGAG GTTGCTTGGG 
     4261 TGACAAGATC TGGAAAGACA GAGCTGGCAG AGCCGATTGC CATCCGTCCT ACTAGTGAAA 
     4321 CAGTAATGTA TCCTGCATAT GCAAAATGGG TGCAGTCCCA CAGAGACCTG CCCATCAGGC 
     4381 TCAACCAGTG GTGCAACGTG GTGCGCTGGG AGTTCAAACA CCCACAACCT TTCCTCCGCA 
     4441 CTCGGGAGTT CCTATGGCAG GAGGGGCACA GCGCCTTTGC CACCTTTGAG GAGGCAGCTG 
     4501 ACGAGGTGTT ACAGATCCTC GAGTTATATG CTCGAGTGTA TGAGGAGCTC TTGGCAATCC 
     4561 CCGTCGTGAG AGGAAGGAAG ACTGAGAAGG AAAAGTTTGC AGGAGGAGAC TATACAACCA 
     4621 CACTAGAAGC CTTCATATCT GCCAGCGGAC GCGCTATCCA GGGAGCAACA TCACACCATT 
     4681 TAGGCCAGAA TTTCTCCAAA ATGTGTGAAA TAGTTTTTGA AGATCCCAAG ACACCAGGAG 
     4741 AAAAGCAGTT TGCATACCAG TGCTCCTGGG GCCTGACAAC TCGCACTATC GGTGTGATGA 
     4801 TCATGGTCCA TGGGGACAAC ATGGGCTTAG TGTTACCTCC CCGTGTAGCG TCTGTCCAGG 
     4861 TGGTAGTCAT TCCCTGTGGA ATCACGAATA CCTTGTCTGA AGAAGACAGA GAGGCCCTGA 
     4921 TGGCAAAATG CAATGAGTAC AGGAGACGGC TGCTCGGCGC TGACATCCGA GCTCGAGTCG 
     4981 ATCTACGAGA CAACTACTCA CCAGGCTGGA AGTTCAACCA CTGGGAGCTC AAGGGTGTTC 
     5041 CAGTCAGACT TGAGGTGGGG CCACGTGATA TGAAGAGCTG TCAGTTTGTA GCAGTCAGAC 
     5101 GAGATACTGG AGAGAAGCTG ACAATTGCTG AAAAGGAGGC GGAGTCTAAA CTGAAAGGGG 
     5161 TTTTGGAAGA CATCCAGCTT AACCTTTTCA CAAGGGCTTC TGAAGACCTT AAATCTCACA 
     5221 TGGTTGTATC GGATACACTG GAAGACTTTC AGAAGGTGCT AGATTCTGGG AAGGTTGCAC 
     5281 AGATTCCATT CTGTGGGGAA ATTGACTGTG AGGACTGGAT CAAAAAGACA ACGGCCAGGG 
     5341 ATCAGGATGT GGAACCTGGT GCTCCATCCA TGGGAGCCAA AAGCCTTTGC ATTCCTTTCA 
     5401 ACCCTCTGTG TGAGCTGCAG CCAGGAGCCA TGTGTGTCTG TGGCAAGAAT CCTGCCAAGT 
     5461 TCTACACCTT GTTTGGTCGG AGTTACACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     5521 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     5581 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     5641 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     5701 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5761 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5821 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5881 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5941 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     6001 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     6061 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     6121 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     6181 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     6241 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     6301 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     6361 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     6421 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     6481 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     6541 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     6601 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     6661 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6721 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6781 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6841 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6901 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6961 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     7021 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     7081 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     7141 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     7201 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     7261 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     7321 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     7381 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     7441 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     7501 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     7561 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     7621 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     7681 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7741 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7801 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7861 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7921 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7981 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     8041 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     8101 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     8161 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     8221 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     8281 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     8341 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     8401 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     8461 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     8521 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     8581 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8641 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8701 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8761 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8821 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8881 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8941 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     9001 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     9061 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     9121 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     9181 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     9241 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     9301 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//