LOCUS       RR205416                8607 bp    DNA     circular  SYN 
DEFINITION  Pitpnm1 (Myc-DDK-tagged ORF) - Rat phosphatidylinositol transfer protein, membrane-associated 1 (Pitpnm1), (10 ug)
ACCESSION   NM_001008369
VERSION     NM_001008369.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4764..4855 
                     /label="Myc-DDK"  
     misc_feature    4885..5470 
                     /label="PolyA signal"  
     misc_feature    4942..4961 
                     /label="Restriction Enzyme"  
     misc_feature    5746..6418 
                     /label="ColE1"  
     misc_feature    6848..7642 
                     /label="Kan/Neo"  
     misc_feature    7677..7979 
                     /label="SV40 ori"  
     misc_feature    8065..8542 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4746..4752 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4746 
                     /label="Pitpnm1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTTATCAAG GAGTACCACA TCTTGCTGCC CATGAGCCTG GACGAGTACC 
     1081 AAGTGGCCCA GCTCTACATG ATCCAGAAAA AGAGCCGGGA GGAATCTAGT GGTGAGGGCA 
     1141 GTGGTGTGGA GATCCTGGCC AACCGGCCCT ACACAGATGG ACCCGGAGGC AATGGGCAGT 
     1201 ACACACACAA GGTGTACCAC GTGGGCTCCC ACATCCCAGG CTGGTTCCGG GCACTGTTGC 
     1261 CCAAGGCTGC TCTGCAGGTA GAAGAGGAAT CTTGGAATGC TTACCCGTAC ACCCGGACAC 
     1321 GGTACACCTG CCCCTTTGTG GAGAAATTCT CCATTGAGAT AGAGACCTAT TACTTGCCTG 
     1381 ATGGGGGGCA GCAGCCTAAC GTCTTCAACT TGAGCGGGGC GGAGAGAAGA CAGAGAATCC 
     1441 TGGATACCAT CGACATCGTG CGGGATGCAG TGGCCCCAGG AGAGTACAAA GCAGAAGAGG 
     1501 ACCCTCGGCT GTACCGCTCA GTCAAGACAG GCCGAGGGCC ACTGGCTGAT GACTGGGCAC 
     1561 GGACAGCAGC CCAGACAGGA CCTCTCATGT GTGCCTATAA GCTGTGCAAG GTTGAATTCC 
     1621 GCTACTGGGG CATGCAGGCC AAGATTGAGC AGTTCATCCA TGATGTAGGT CTGCGCAGGG 
     1681 TAATGCTTCG GGCCCATCGC CAGGCCTGGT GTTGGCAGGA TGAGTGGATT GAACTGAGCA 
     1741 TGGCTGACAT CCGGGCACTG GAAGAGGAGA CAGCACGTAT GCTGGCCCAG CGTATGGCTA 
     1801 AGTGCAACAC TGGCAGTGAG GGACCGGAGG CCCAGACCCC TGGGAAACCC AGCACTGAGA 
     1861 CCCAGCCTGG GACCAGAACG GCTGGCACCC CTGATGGGCC TGAGGCCCCT CCTGGCCCCG 
     1921 ACGCCTCACC AGATGCCAGC TTTGGGAAGC AGTGGTCCTC ATCTTCCCGT TCCTCCTACT 
     1981 CATCCCAACA TGGAGGTGGT GTGTCTCCAC AGAGCTTGTC TGAGTGGCGC ATGCAGAACA 
     2041 TTGCCCGGGA CTCTGAGAAC AGCTCCGAGG AAGAATTCTT TGATGCCCAT GAAGGTTTCT 
     2101 CAGACAGTGA TGAGGTCTTC CCCAAGGAGA TGACCAAGTG GAACTCCAAT GATTTTATTG 
     2161 ATGCCTTTGC CTCCCCGACT GAGGTAGAGG GGGTGCCAGA TCCTGCAATC ATGGCTACCA 
     2221 AAGGCATTGA AGATGAGGCC CGAGCTCCCA GGGACTCAGA GGGCCTGGAT GGAACCGGGG 
     2281 ATCTAGGGGT TGAGGCATGC TCTGTGCATG CCCTCTTCCT CATCCTACAC AGCGGCAGCA 
     2341 TCCTGGACTC TGGCCCTGGA GACACCAACT CCAAGCAGGC CGATGTTCAA ACACTGAGCA 
     2401 CAGCCTTTGA GGCCGTCACC CGAGTCCATT TCCCCGAGGC CCTGGGTCAC GTGGCACTGC 
     2461 GGCTGGTGCC CTGTCCACCC ATCTGCGCGG CTGCCTATGC TCTTGTCTCC AACCTGAGCC 
     2521 CCTACAGCCA TGATGGGGAC AGCCTGTCCC GCTCCCAGGA CCACATTCCA CTGGCTGCTC 
     2581 TGCCACTGCT GGCCACCTCA TCCTCCCGCT ACCAGGGTGC CGTGGCCACT GTCATCGCTC 
     2641 GCACCAACCA GGCCTATGCA GCCTTCCTGC GCTCATCGGA GGGCACAGGT TTCTGCGGGC 
     2701 AGGTGGTGCT GATCGGAGAT GGTGTTGGTG GCATCCTGGG CTTTGATGCG CTCTGCCACA 
     2761 GTGCCAGTGC AGGCACAGGG AGTCGAGGCA GCAGCCGCCG TGGGAGCATG AACAATGAGA 
     2821 TGCTCTCCCC AGAAGTTGGT CCAGTACGGG ACCCGCTGGC AGATGGGGTG GAGGTACTGG 
     2881 GTCGTGCTAG CCCAGAACCC TCAGCCCTAC CTGCTCAGCG CACCTCCAGC GACATGGCTA 
     2941 ACCCGGATCC TGATGGCTCT CAGAACAGCC TACAGGTAGC CCCCACAGTT ACCTCTGGGG 
     3001 AGCCCCGTCG TGCAAGCACA GCCTCCTGTC CACCTGCCAG TTCTGAGGCT CCTGACGGCC 
     3061 CCACTAATGC TGCTCGACTG GACTTCAAGG TCTCAGGCTT CTTCCTCTTT GGCTCCCCAC 
     3121 TGGGCTTGGT GCTGGCTCTA CGCAAAACCG TCATGCCTGC CTTGGAGGTG GCTCAGATGC 
     3181 GTCCAGCCTG CGAACAGATC TACAATCTCT TCCATGCCGC TGACCCCTGT GCCTCCCGCC 
     3241 TGGAACCCCT GCTGGCCCCC AAGTTCCAGG CCATCGCCCC CCTGGCTGTA CCTCGTTACC 
     3301 AGAAGTTTCC CCTAGGAGAT GGCTCATCCC TGCTGCTGGC TGACACTTTG CAGACCCATT 
     3361 CGAGCCTCTT CCTGGAAGAG CTGGAGATGA TGGTGCCCTC CACGCCCACC TCGGCCAGTG 
     3421 GTGCCTTCTG GAAGGGCAAT GAATTAGGCA ATGAGCCGGC AGCCCAGCCA GCAGCCCCCA 
     3481 GCACCACCAG TGAGGTGGTT AAGATCCTGG ATCGCTGGTG GGGAAACAAG CGCATCGACT 
     3541 ATTCACTGTA CTGCCCTGAG GCACTCACCG CCTTTCCCAC CGTCACGCTG CCCCACCTCT 
     3601 TCCACGCCAG CTACTGGGAG TCGGCAGACG TAGTGGCCTT CATTCTGCGC CAGGTCATTG 
     3661 AGAAGGAGCG GCCACAGTTG ACAGAATGTG AGGAGCCATC CATCTACAGC CCTGCCTTCC 
     3721 CCAGGGAGAA GTGGCAGCGC AAACGCACAC AGGTCAAGAT CCGGAACGTC ACTTCCAACC 
     3781 ATCGGGCAAG TGACACCGTG GTATGCGAGG GCCGTCCCCA GGTGCTGAAT GGGCGCTTCA 
     3841 TGTACGGACC ACTGGACGTG GTCACACTCA CTGGCGAGAA GGTGGATGTC TATGTCATGA 
     3901 CACAGCCCCT GTCGGGCAAG TGGATCCACT TTGGCACAGA GGTCACCAAC AGCTCAGGCC 
     3961 GTCTCACCTT CCCAGTGCCC TCAGAACGTG CACTGGGCAT TGGTGTCTAC CCTGTGCGCA 
     4021 TGGTGGTCAG GGGAGACCAT ACCTACGCCG AGTGCTGTCT GACTGTGGTG TCCCGAGGCA 
     4081 CAGAAGCTGT GGTCTTCAGC ATCGACGGCT CATTCACTGC CAGTGTCTCT ATCATGGGCA 
     4141 GTGACCCCAA GGTGCGCGCT GGCGCAGTGG ACGTGGTCAG GCACTGGCAG GACTCAGGCT 
     4201 ACTTGATCGT GTATGTAACT GGCCGGCCTG ACATGCAGAA GCACCGTGTA GTGGCCTGGC 
     4261 TGTCGCAACA CAACTTCCCC CATGGTGTTG TCTCCTTCTG TGATGGCCTC ACCCATGACC 
     4321 CACTGCGACA GAAAGCTATG TTTCTGCAGA GCCTGGTGCA AGAGGTAGAA CTGAACATCG 
     4381 TGGCTGGGTA TGGGTCACCG AAAGACGTTG CAGTATATGC GGCCCTGGGA CTCTCTCCAA 
     4441 GCCAGACCTA CATTGTTGGT CGTGCAGTGC GTAAACTGCA GGCACAGTGT CAGTTCCTGT 
     4501 CAGATGGCTA TGTGGCCCAT TTGGGCCAGC TGGAGGCAGG CTCCCACTCC CATGCCCCCT 
     4561 CAGGACCTCC AAGAGCTGCT CTGGCCAAGA GCAGCTATGC TGTGGCTGCC CCTGTGGACT 
     4621 TCCTCCGTAA GCAGAGCCAG CTGCTTCGAT CCAGAGGCCC CAGCCAGGTG GACCTCGAGG 
     4681 GCCCAGGAAC ACCTCCTACC ACCCTGGCCA GGGGCAAGAC TCGAAGCATC AGCCTCAAGC 
     4741 TAGACAGTGA AGAGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4801 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4861 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4921 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4981 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5041 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5101 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5161 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5221 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5281 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5341 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5401 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5461 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5521 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5581 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5641 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5701 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5761 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5821 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5881 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5941 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6001 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6061 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6121 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6181 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6241 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6301 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6361 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6421 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6481 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6541 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6601 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6661 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6721 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6781 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6841 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6901 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6961 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7021 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7081 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7141 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7201 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7261 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7321 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7381 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7441 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7501 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7561 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7621 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7681 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7741 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7801 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7861 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7921 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7981 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8041 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8101 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8161 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8221 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8281 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8341 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8401 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8461 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8521 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8581 ATTTAACAAA AATTTAACGC GAATTTT
//