LOCUS       RR205358                8715 bp    DNA     circular  SYN 
DEFINITION  Abcb4 (Myc-DDK-tagged ORF) - Rat ATP-binding cassette, sub-family B (MDR/TAP), member 4 (Abcb4), (10 ug)
ACCESSION   NM_012690
VERSION     NM_012690.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4872..4963 
                     /label="Myc-DDK"  
     misc_feature    4993..5578 
                     /label="PolyA signal"  
     misc_feature    5050..5069 
                     /label="Restriction Enzyme"  
     misc_feature    5854..6526 
                     /label="ColE1"  
     misc_feature    6956..7750 
                     /label="Kan/Neo"  
     misc_feature    7785..8087 
                     /label="SV40 ori"  
     misc_feature    8173..8650 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4854..4860 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4854 
                     /label="Abcb4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATCTTGAG GCAGCAAGAA ACGGAACAGC GCGGCGCCTG GACGGCGACT 
     1081 TTGAACTAGG CAGCATCAGC AACCAGAGCA GAGAAAAAAA GAAGAAAGTG AATTTAATTG 
     1141 GCCCGTTGAC ACTGTTCCGA TACTCTGATT GGCAGGATAA ATTGTTTATG CTCCTGGGCA 
     1201 CCGCCATGGC CATAGCTCAC GGATCAGGTC TTCCCCTTAT GATGATAGTC TTTGGAGAAA 
     1261 TGACAGATAA GTTTGTAGAT AATGCTGGGA ACTTTTCCTT GCCAGTGAAT TTTTCATTGT 
     1321 CAATGCTAAA TCCAGGAAGA ATTCTGGAAG AAGAAATGAC TAGATATGCA TACTACTATT 
     1381 CGGGACTAGG TGGTGGAGTT CTTTTGGCTG CCTATATCCA AGTCTCCTTC TGGACTTTGG 
     1441 CAGCTGGCCG ACAAATAAGG AAAATCAGGC AAAAATTTTT TCACGCCATC CTTCGACAAG 
     1501 AAATGGGCTG GTTTGATATC AAGGGCACCA CCGAACTCAA CACGCGGCTG ACAGATGACA 
     1561 TCTCCAAAAT CAGTGAAGGA ATTGGTGACA AGGTTGGAAT GTTCTTTCAA GCAATAGCCA 
     1621 CGTTTTTTGC AGGATTCATA GTGGGGTTCA TCAGAGGCTG GAAACTCACC CTCGTGATCA 
     1681 TGGCCATCAC CGCCATCTTG GGGCTCTCTA CAGCCGTTTG GGCAAAGATA CTCTCAACAT 
     1741 TCAGTGACAA AGAACTAGCT GCCTATGCAA AAGCAGGTGC CGTGGCGGAA GAGGCTCTGG 
     1801 GAGCCATCAG GACCGTGATA GCTTTCGGGG GCCAGAACAA AGAGCTAGAA AGGTATCAGA 
     1861 AGCATTTAGA AAATGCCAAA AAGATTGGAA TTAAAAAGGC TATCTCGGCC AACATCTCCA 
     1921 TGGGCATTGC CTTTTTGTTA ATATATGCAT CCTATGCACT GGCCTTCTGG TATGGATCCA 
     1981 CTCTGGTTAT ATCAAAAGAA TATACAATTG GAAATGCCAT GACAGTGTTC TTCTCAATCC 
     2041 TCATTGGGGC CTTCAGTGTG GGGCAGGCTG CCCCCTGTAT TGATGCTTTC CCCAATGCTA 
     2101 GAGGAGCAGC CTATGTGATC TTTGACATTA TTGATAATAA TCCTAAAATT GACAGTTTTT 
     2161 CAGAGAGAGG ACACAAGCCA GACAGCATCA AAGGAAATTT GGAGTTCAGT GACGTTCACT 
     2221 TTTCCTACCC ATCTCGGGCT AATATCAAGA TCTTGAAGGG CCTCAACCTG AAGGTGAAGA 
     2281 GCGGGCAGAC GGTAGCCCTG GTTGGCAACA GTGGCTGTGG GAAAAGCACA ACTGTCCAGC 
     2341 TGCTGCAGAG GCTCTACGAC CCCACAGAGG GTACGATTAG CATCGATGGG CAGGACATCC 
     2401 GGAACTTTAA CGTCAGGTGT CTAAGGGAAT TCATCGGCGT GGTGAGTCAA GAGCCGGTAC 
     2461 TGTTCTCTAC CACGATTGCT GAAAATATCC GCTATGGCCG TGGGAATGTA ACAATGGATG 
     2521 AGATTAAAAA AGCTGTCAAA GAGGCTAATG CCTATGACTT CATCATGAAA CTGCCACAGA 
     2581 AATTTGACAC CCTGGTTGGT GACAGAGGGG CGCAGCTGAG CGGGGGACAG AAACAGAGGA 
     2641 TCGCCATTGC TCGTGCCTTG GTCCGCAACC CCAAGATCCT CCTGCTGGAC GAGGCCACGT 
     2701 CAGCCTTGGA CACAGAAAGC GAAGCTGAGG TGCAGGCCGC TCTGGATAAG GCCAGAGAAG 
     2761 GCCGGACCAC CATCGTGATA GCTCACCGAC TGTCAACTGT CCGGAATGCA GATGTCATCG 
     2821 CTGGGTTTGA GGATGGCGTC ATCGTGGAGC AAGGAAGCCA CAGTGAGCTG ATAAAGAAGG 
     2881 AAGGGATCTA CTTCAGACTT GTTAACATGC AGACATCAGG AAGCCAGATC CTGTCAGAAG 
     2941 AATTTGAAGT TGAGCTAAGT GATGAAAAGG CTGCTGGAGG TGTGGCCCCA AATGGCTGGA 
     3001 AAGCACGCAT ATTTAGGAAT TCTACGAAGA AAAGTCTGAA AAGTTCACGG GCGCATCAAA 
     3061 ATAGGCTGGA TGTGGAAACC AATGAACTTG ATGCAAACGT GCCACCAGTG TCTTTTCTGA 
     3121 AGGTCTTAAG ACTGAATAAA ACAGAGTGGC CCTACTTTGT GGTGGGGACA CTCTGTGCCA 
     3181 TTGCCAACGG GGCCCTCCAG CCGGCATTCT CCATCATCCT GTCAGAGATG ATAGCTATCT 
     3241 TTGGCCCTGG GGATGACACA GTAAAGCAAC AGAAGTGTAA CATGTTCTCG CTGGTCTTCT 
     3301 TGGGCCTAGG AGTCCACTCC TTCTTTACTT TCTTCCTTCA GGGTTTCACA TTCGGGAAAG 
     3361 CTGGCGAGAT CCTCACCACA AGGCTCCGGT CCATGGCCTT CAAAGCAATG CTAAGACAGG 
     3421 ACATGAGCTG GTTTGACGAT CATAAAAACA GTACTGGTGC CCTCTCTACA AGACTCGCCA 
     3481 CAGACGCTGC GCAGGTCCAA GGAGCCACAG GAACCAGGTT GGCTTTAATT GCACAGAACA 
     3541 CAGCCAACCT TGGAACGGGT ATTATTATAT CATTTATTTA CGGTTGGCAA CTGACACTTC 
     3601 TGCTCTTATC AGTTGTTCCA TTCATTGCTG TAGCGGGAAT TGTTGAAATG AAAATGTTGG 
     3661 CTGGCAACGC CAAGAGAGAT AAAAAGGAGA TGGAAGCTGC TGGAAAGATT GCAACAGAGG 
     3721 CAATAGAAAA TATTCGGACT GTTGTATCCT TGACCCAAGA GAGAAAATTT GAGTCAATGT 
     3781 ATGTTGAAAA ATTACACGGA CCTTACAGGA ATTCAGTGCG GAAGGCTCAC ATCTACGGCA 
     3841 TCACTTTTAG CATCTCACAA GCATTCATGT ACTTTTCTTA TGCTGGCTGC TTTCGATTTG 
     3901 GTTCTTACCT CATTGTGAAT GGACACATGC GCTTCAAGGA TGTCATCCTG GTGTTCTCAG 
     3961 CAATCGTGCT TGGTGCAGTG GCTCTAGGAC ATGCCAGCTC ATTTGCTCCA GACTATGCAA 
     4021 AAGCCAAGCT GTCTGCAGCA TACTTATTCA GTCTGTTTGA AAGACAACCT CTGATTGACA 
     4081 GCTACAGCAG AGAAGGAATG TGGCCGGATA AGTTTGAAGG AAGCGTGACA TTCAATGAAG 
     4141 TTGTGTTCAA TTATCCCACC CGGGCCAATG TGCCAGTGCT TCAGGGGCTG AGCCTCGAGG 
     4201 TGAAGAAGGG GCAGACCCTG GCCCTGGTGG GCAGTAGTGG CTGCGGGAAG AGCACCGTGG 
     4261 TCCAGCTGCT CGAGCGCTTC TACGACCCCA TGGCCGGAAC AGTGCTCCTC GATGGTCAGG 
     4321 AAGCAAAGAA ACTCAATGTC CAGTGGCTCC GAGCTCAACT TGGCATTGTG TCCCAGGAGC 
     4381 CCATCCTGTT TGACTGCAGC ATCGCCAAGA ACATCGCCTA CGGAGACAAC AGCCGTGTCG 
     4441 TGTCTCAGGA TGAGATTGTG AGGGCGGCCA AGGAGGCCAA CATCCACCCC TTCATTGAGA 
     4501 CACTGCCCCA AAAGTATGAA ACAAGAGTAG GAGACAAGGG GACACAGCTC TCTGGAGGCC 
     4561 AGAAACAGAG GATTGCTATC GCCCGAGCCC TCATCAGACA GCCTCGGGTC CTACTGCTGG 
     4621 ATGAAGCCAC GTCGGCTTTG GACACTGAGA GTGAAAAGGT CGTCCAGGAA GCGCTGGACA 
     4681 AAGCCAGGGA AGGCCGCACC TGCATTGTGA TCGCGCACCG CCTGTCCACC ATCCAGAACG 
     4741 CAGACTTGAT CGTGGTGATC GACAACGGCA AGGTCAAGGA GCACGGCACC CACCAGCAGC 
     4801 TGCTGGCCCA GAAAGGCATC TATTTCTCCA TGGTCAACAT TCAAGCTGGC ACACAGAACT 
     4861 TAACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4921 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4981 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5041 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5101 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5161 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5221 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5281 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5341 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5401 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5461 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5521 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5581 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5641 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5701 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5761 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5821 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5881 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5941 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6001 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6061 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6121 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6181 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6241 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6301 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6361 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6421 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6481 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6541 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6601 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6661 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6721 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6781 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6841 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6901 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6961 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7021 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7081 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7141 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7201 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7261 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7321 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7381 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7441 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7501 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7561 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7621 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7681 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7741 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7801 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7861 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7921 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7981 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8041 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8101 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8161 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8221 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8281 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8341 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8401 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8461 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8521 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8581 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8641 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8701 TTTAACGCGA ATTTT
//