LOCUS       RR205331                8178 bp    DNA     circular  SYN 
DEFINITION  Itga5 (Myc-DDK-tagged ORF) - Rat integrin alpha 5 (fibronectin receptor alpha) (Itga5), (10 ug)
ACCESSION   NM_001108118
VERSION     NM_001108118.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4335..4426 
                     /label="Myc-DDK"  
     misc_feature    4456..5041 
                     /label="PolyA signal"  
     misc_feature    4513..4532 
                     /label="Restriction Enzyme"  
     misc_feature    5317..5989 
                     /label="ColE1"  
     misc_feature    6419..7213 
                     /label="Kan/Neo"  
     misc_feature    7248..7550 
                     /label="SV40 ori"  
     misc_feature    7636..8113 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4317..4323 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4317 
                     /label="Itga5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCCCCCGC CAGCCGACCC GGCGGCCGGG GGGAGGGCCC AGCCGGGAGT 
     1081 TGGGCAAACT CCTCCCCGCG TTGAGTCATT CGCCTCTGGG AGGTTTAGGA AGCGGCTCCG 
     1141 GGTCGGTGGC CCCAGGACAG GGAAGAACGG GCGCTATGGG GAGCTGGACG CCACGGTCGC 
     1201 CACGATCTCC TCTCCATGCG GTGCTGCTGC GCTGGGGGCC CCGACGCCTA CCGCCGCTGC 
     1261 TGCCGCTGCT GCTGCTACTG TGGCCGCCAC CACTCCAGGT TGGGGGCTTC AACCTAGACG 
     1321 CGGAGGCCCC GGCGGTGCTC TCCGGGCCCC CCGGCTCCCT CTTTGGCTTC TCCGTGGAGT 
     1381 TTTACCGGCC GGGAAGGGAC GGAGTCAGTG TGCTGGTTGG GGCACCCAAG GCTAACACTA 
     1441 GCCAACCAGG AGTACTACAA GGTGGCGCTG TCTATGTGTG TCCCTGGGGC ACCAGTCCTA 
     1501 TCCAGTGCAG CACCATTCAA TTTGACAGCA AAGGCTCCCG CATCCTGGAG TCTTCATTGT 
     1561 ATAGTGAGGA ACCTGTGGAG TACAAGTCCT TGCAGTGGTT CGGGGCAACG GTTCGGGCCC 
     1621 ACGGCTCCTC CATCTTGGCA TGCGCTCCAC TGTATAGCTG GCGCACAGAA AAGGACCCAC 
     1681 AGAATGACCC AGTGGGCACC TGCTACCTCT CCACAGAAAA CTTCACCCGG ATTCTGGAGT 
     1741 ATGCACCTTG CCGCTCAGAT TTTGGCAGTG CAGCAGGGCA GGGTTACTGC CAAGGGGGCT 
     1801 TCAGTGCCGA GTTCACCAAG ACTGGCCGCG TGGTCCTAGG AGGACCTGGG AGCTACTTCT 
     1861 GGCAAGGCCA GATCCTGTCG GCCACTCAAG AGCAGATCTC GGAGTCCTAT TACCCACAGT 
     1921 ACCTCATCAA CCCTGTTCAG GGGCAGCTGC AGACCCGCCA GGCCAGCTCC GTCTATGATG 
     1981 ACAGCTACTT GGGATACTCT GTGGCTGTAG GTGAATTCAG TGGTGATGAC ACAGAAGACT 
     2041 TTGTTGCTGG CGTGCCCAAA GGAAACCTCA CCTATGGCTA TGTCACTGTC CTTAATGGCT 
     2101 CAGACATCCA CTCCCTCTAC AACGTCTCAG GAGAACAGAT GGCCTCCTAT TTTGGCTATG 
     2161 CCGTAGCTGC CACTGATACC AATGGAGATG GGCTAGATGA CCTGCTGGTA GGGGCACCCC 
     2221 TACTCATGGA GCGGACAGCT GACGGGAGAC CTCAGGAGGT GGGCAGGGTC TACATCTATC 
     2281 TGCAGCACCC AGAGGGTATA GAGCCTACGC CCTCCCTCAC CCTCACTGGC CAAGATGAGT 
     2341 TCGGCCGATT CGGCAGCTCC TTGACACCCC TGGGGGACCT GGACCAAGAC GGCTACAATG 
     2401 ATGTCGCCAT TGGGGCTCCG TTTGGTGGGG AGGCCCAGCA AGGAGTTGTA TTTATATTCC 
     2461 CGGGAGGCCC AGGAGGACTG AACACTAAGC CTTCCCAGGT TTTGCAGCCT CTGTGGGCAG 
     2521 CTGGCCATAC CCCAGACTTC TTTGGCTCTG CCCTCCGAGG AGGACGAGAT CTGGACGGCA 
     2581 ATGGATACCC TGATCTAATC GTTGGATCCT TTGGTGTGGA CAAGGCTCTG GTATACAGGG 
     2641 GTCGGCCCAT CATATCTGCC AGTGCGTCTC TCACCATCTT CCCCTCCATG TTCAACCCAG 
     2701 AGGAGCGCAG CTGCAGCCTG GAAGGAAACC CTGTATCCTG CATCAACCTT AGCTTCTGCC 
     2761 TCAACGCCTC TGGAAAACAT GTACCCAACT CTATAGGCTT CGAGGTGGAA CTCCAACTGG 
     2821 ACTGGCAGAA GCAGAAGGGA GGGGTCCGGA GGGCACTGTT CCTGGCTTCC AAGCAGGCCA 
     2881 CCCTTACCCA GACCCTGCTT ATCCAGAATG GGGCTCGGGA GGACTGCAGG GAGATGAAGA 
     2941 TCTACCTCAG GAATGAATCA GAATTCAGAG ACAAACTCTC CCCAATTCAC ATTGCCCTCA 
     3001 ACTTCTCCTT GGACCCCAAA GCCCCCATGG ACAGCCATGG CCTCCGGCCA GTCCTACACT 
     3061 ACCAGAGCAA GAGCCGGATA GAGGACAAGG CCCAGATCTT GCTAGACTGT GGTGAAGACA 
     3121 ACATCTGTGT GCCTGACCTG CAGCTGGCTG TGTATGGGGA GAAGAAACAT GTGTACCTGG 
     3181 GTGACAAGAA CGCCCTGAAC CTGACATTCC TCGCCCAGAA TCTGGGCGAG GGTGGGGCCT 
     3241 ATGAAGCCGA GCTGCGGGTC ACAGCCCCCC TGGAGGCTGA GTACTCGGGG CTTGTCAGAC 
     3301 ACCCAGGGAA CTTCTCCAGC CTGAGCTGTG ACTACTTCGC GGTGAACCAG AGCCGCCAGC 
     3361 TGGTGTGTGA CCTGGGCAAC CCCATGAAGG CAGGCACCAG TATCTGGGGT GGCCTTCGGT 
     3421 TCACTGTTCC TCATCTTCAA GACACAAAGA AAACCATCCA ATTTGACTTC CAGATCCTCA 
     3481 GCAAGAACCT GAACAACTCA CAAAGCAACA TGGTCTCCTT CCCACTCTCG GTGGAGGCTC 
     3541 AAGCCCAAGT CTCCCTTAAC GGTGTCTCCA AGCCTGAAGC CGTGATTTTC CCAGTCAGCG 
     3601 ACTGGAATCC TCAAGACCAG CCTCAGAAGG AGGGAGATTT GGGCCCTGCT GTCCACCATG 
     3661 TCTATGAGCT CATTAACCAG GGGCCCAGCT CCATCAGCCA GGGTGTGCTG GAGATCAGCT 
     3721 GTCCCCAGGC TCTGGAAGGC CAACAGCTCC TCTATGTGAC CAAGGTGACG GGACTCAACA 
     3781 ACTGCACCTC CAACTACACC CCCAACTCAC AGGGCCTGGA GTTGGATCCA GAGGTTTCTC 
     3841 CACATCACCT GCAGAGGCGA GAGGCTCCAG GGAGGAGTTC TACCACCTCA GGAACACAAG 
     3901 TCCTGAAATG CCCTGAAGCC AAGTGTTTCA GGCTGCGCTG TGAGTTTGGG CCACTGCATC 
     3961 GCCAAGAGAG CCGGAGTCTG CAGCTGCATT TCCGAGTCTG GGCCAAGACC TTCTTGCAGC 
     4021 AGGAATACCA GCCGTTCAGC CTTCAGTGTG AGGCTCTATA TGAAGCCCTG AAGATGCCCT 
     4081 ACCAAATCCT GCCTCGGCAG CTGCCCCAAA AGAAACTCCA GGTGGCCACA GCCGTGCAGT 
     4141 GGACCAAGGC AGAAGGCAGC AATGGCGTCC CACTGTGGAT CATCATCCTC GCCATCCTTT 
     4201 TTGGCCTCCT GCTCCTAGGT CTGCTCATCT ATGTCCTCTA CAAGCTCGGC TTCTTCAAAC 
     4261 GCTCCCTCCC CTACGGCACA GCCATGGAAA AAGCTCAGCT CAAGCCTCCA GCCACCTCAG 
     4321 ATGCCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4381 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4441 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4501 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4561 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4621 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4681 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4741 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4801 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4861 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4921 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4981 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5041 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5101 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5161 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5221 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5281 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5341 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5401 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5461 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5521 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5581 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5641 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5701 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5761 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5821 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5881 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5941 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6001 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6061 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6121 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6181 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6241 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6301 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6361 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6421 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6481 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6541 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6601 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6661 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6721 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6781 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6841 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6901 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6961 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7021 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7081 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7141 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7201 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7261 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7321 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7381 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7441 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7501 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7561 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7621 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7681 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7741 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7801 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7861 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7921 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7981 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8041 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8101 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8161 AAATTTAACG CGAATTTT
//