LOCUS       RR205298                8712 bp    DNA     circular  SYN 
DEFINITION  Magi2 (Myc-DDK-tagged ORF) - Rat membrane associated guanylate kinase, WW and PDZ domain containing 2 (Magi2), (10 ug)
ACCESSION   NM_053621
VERSION     NM_053621.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4869..4960 
                     /label="Myc-DDK"  
     misc_feature    4990..5575 
                     /label="PolyA signal"  
     misc_feature    5047..5066 
                     /label="Restriction Enzyme"  
     misc_feature    5851..6523 
                     /label="ColE1"  
     misc_feature    6953..7747 
                     /label="Kan/Neo"  
     misc_feature    7782..8084 
                     /label="SV40 ori"  
     misc_feature    8170..8647 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4851..4857 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4851 
                     /label="Magi2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCAAAAGC TTGAAAAAGA AAAGCCACTG GACTAGCAAA GTCCACGAGA 
     1081 GTGTCATTGG CAGAAACCCG GAGGGCCAGC TGGGCTTTGA ACTGAAGGGG GGCGCCGAAA 
     1141 ACGGACAGTT CCCCTACCTG GGGGAGGTGA AGCCGGGCAA GGTGGCCTAT GAAAGCGGCA 
     1201 GCAAGTTGGT GTCCGAGGAG CTGCTGCTGG AGGTGAACGA GACCCCCGTG GCGGGGCTCA 
     1261 CCATCAGGGA TGTTCTGGCC GTGATCAAAC ACTGCAAGGA CCCCCTCCGG CTCAAGTGTG 
     1321 TCAAGCAAGG AGGAATTGTT GATAAGGATC TTCGTCACTA CCTCAACTTA AGATTTCAGA 
     1381 AGGGTTCTGT TGACCACGAA CTACAGCAAA TCATCCGTGA CAACCTCTAC CTCCGCACAG 
     1441 TGCCATGCAC CACAAGGCCA CATAAGGAGG GTGAGGTCCC TGGAGTGGAC TACATTTTCA 
     1501 TAACCGTTGA GGACTTTATG GAATTGGAGA AAAGCGGTGC TCTCCTAGAA AGTGGGACCT 
     1561 ATGAAGACAA CTATTACGGC ACTCCAAAGC CTCCAGCTGA ACCAGCACCA TTATTATTAA 
     1621 ATGTAACAGA TCAGATACTC CCGGGAGCTA CTCCAAGTGC TGAGGGGAAG CGGAAAAGAA 
     1681 ATAAGTCTGT GACCAACATG GAGAAAGCAA GTATAGAGCC ACCGGAGGAA GAGGAGGAAG 
     1741 AAAGGCCTGT GGTCAACGGA AATGGCGTCG TCATAACACC AGAATCCAGT GAACATGAAG 
     1801 ACAAAAGTGC AGGTGCCTCA GGGGAGACCC CCTCCCAGCC TTACCCTGCA CCAGTGTACA 
     1861 GTCAGCCTGA AGAGCTCAAG GACCAGATGG ATGATACAAA GTCAACAAAG CCCGAGGAGA 
     1921 ATGAGGACTC CGACCCATTG CCTGATAACT GGGAAATGGC CTACACAGAG AAGGGTGAAG 
     1981 TCTACTTCAT TGACCATAAC ACAAAGACAA CATCATGGCT GGATCCACGA CTTGCCAAAA 
     2041 AGGCTAAGCC TGCAGAGGAG TGCAAGGAAA ATGAGCTTCC ATATGGCTGG GAAAAAATCG 
     2101 ATGATCCTAT ATATGGCACT TACTATGTCG ACCACATAAA TAGAAGAACA CAGTTTGAAA 
     2161 ACCCTGTCCT GGAAGCAAAA AGGAAGCTAC AGCAACATAA CATGCCCCAC ACAGAACTTG 
     2221 GAACAAAGCC CCTGCAGGCC CCAGGTTTCC GAGAAAAGCC ACTCTTCACC CGGGATGCAT 
     2281 CCCAGTTGAA GGGAACATTC CTCAGCACCA CCCTCAAAAA GAGCAACATG GGCTTTGGGT 
     2341 TTACGATCAT CGGTGGAGAT GAGCCGGACG AGTTTCTACA GGTGAAGAGT GTGATCCCGG 
     2401 ATGGGCCCGC CGCACAGGAT GGGAAAATGG AGACAGGTGA TGTCATTGTC TATATTAATG 
     2461 AAGTTTGTGT CCTTGGACAC ACTCATGCAG ATGTTGTCAA ACTTTTCCAG TCTGTTCCTA 
     2521 TTGGTCAGAG CGTCAACCTG GTGTTGTGTC GTGGCTACCC TTTGCCCTTT GATCCTGAAG 
     2581 ACCCTGCCAA CAGCATGGTG CCACCCCTTG CAATAATGGA GAGGCCACCT CCGGTGATGG 
     2641 TCAATGGAAG ACATAACTAT GAAACATATT TGGAATACAT TTCTCGGACC TCACAGTCGG 
     2701 TCCCAGATAT TACAGACCGG CCACCTCATT CTTTGCACTC CATGCCAGCT GATGGCCAAC 
     2761 TAGATGGCAC ATATCCACCA CCCGTCCACG ATGACAATGT GTCTGTGGCT TCATCTGGAG 
     2821 CCACTCAAGC TGAACTTATG ACCTTAACCA TTGTGAAAGG TGCCAAGGGA TTTGGCTTTA 
     2881 CTATTGCCGA CAGTCCCACG GGACAGCGGG TGAAACAAAT ACTTGACATT CAGGGATGCC 
     2941 CCGGGCTGTG TGAAGGAGAC CTCATTGTTG AGATCAATCA ACAGAATGTA CAGAACCTGA 
     3001 GCCATACAGA AGTGGTTGAT ATACTTAAGG ACTGCCCCGT CGGAAGCGAG ACTTCTCTAA 
     3061 TCATCCATCG AGGAGGTTTC TTTTCTCCAT GGAAAACTCC AAAGCCTATG GTGGACCGGT 
     3121 GGGAGAACCA AGGCAGCCCT CAAACAAGTT TATCTGCTCC GGCCGTCCCA CAGAGCCTGC 
     3181 CCTTCCCACC TGCCCTTCAT AGGAGCTCCT TTCCTGACTC AACAGAGGCC TTTGACCCAC 
     3241 GGAAGCCTGA CCCGTATGAG CTCTACGAGA AATCGAGAGC CATTTATGAA AGTAGGCAAC 
     3301 AAGTGCCACC CAGGACCAGT TTTCGAATGG ATTCCTCTGG TCCAGATTAT AAGGAATTGG 
     3361 ATGTTCACCT TCGGAGGATG GAGTCTGGAT TTGGCTTCAG AATCCTTGGG GGAGACGAGC 
     3421 CCGGACAGCC TATTTTGATC GGAGCTGTCA TTGCGATGGG CTCAGCTGAC AGAGACGGCC 
     3481 GTCTGCACCC AGGAGACGAG CTTGTCTACG TGGATGGGAT CCCGGTGGCT GGCAAGACCC 
     3541 ACCGGTATGT CATCGACCTC ATGCACCATG CGGCCCGCAA CGGGCAGGTT AACCTCACTG 
     3601 TGAGAAGAAA GGTGCTATGT GGAGGGGAGC CCTGCCCAGA GAATGGGAGG AGTCCAGGCT 
     3661 CTGTATCAAC ACACCACAGC TCTCCCCGCA GTGACTATGC CACCTACGCC AACAGCAACC 
     3721 ACGCGGCCCC CAGTAACAAT GCCTCACCCC CTGAAGGCTT TGCCTCGCAC AGCCTGCAGA 
     3781 CCAGTGACGT CATCATTCAC CGAAAGGAAA ACGAAGGCTT CGGCTTCGTC ATCATCAGCT 
     3841 CCCTGAACAG GCCTGAGTCT GGAGCCACCA TAACCGTGCC CCATAAAATC GGACGAATCA 
     3901 TCGATGGGAG CCCTGCAGAT CGCTGCGCAA AACTCAAAGT GGGCGACCGG ATCTTAGCAG 
     3961 TCAACGGCCA GTCTATCATC AACATGCCTC ACGCTGACAT CGTGAAGCTC ATCAAGGATG 
     4021 CGGGTCTCAG CGTCACCCTC CGCATCATTC CTCAGGAGGA GCTCAACAAC CCAACATCAG 
     4081 CACCCAGTTC AGAGAAGCAG AGCCCCATGG CCCAGCAGCA CAGCCCTCTG GCCCAGCAGC 
     4141 ACAGCCCTCT GGCCCAGCCG AGCCCAGCCA CCCCCAACAG CCCAGTTGCA CAGCCAGCTC 
     4201 CTCCTCAACC TCTCCAGCTG CAAGGACATG AAAATAGTTA CAGGTCAGAA GTTAAAGCAA 
     4261 GGCAAGATGT GAAGCCAGAC ATCCGGCAGC CGCCCTTCAC AGACTACAGG CAGCCGCCAC 
     4321 TGGACTACAG GCAACCCCCG GGAGGGGACT ACTCGCAGCC CTCACCCTTG GACTACAGGC 
     4381 AGCACTCCCC AGACACCAGG CAGTACCCTC TGTCAGACTA CAGGCAGCCA CAGGATTTCG 
     4441 ATTATTTCAC TGTGGACATG GAGAAAGGAG CCAAAGGATT TGGATTCAGC ATCCGTGGAG 
     4501 GAAGGGAATA CAAAATGGAT CTGTACGTGT TGAGATTGGC GGAGGATGGG CCAGCCATAA 
     4561 GGAACGGCAG GATGAGGGTG GGAGATCAGA TCATCGAAAT AAATGGGGAA AGCACACGAG 
     4621 ACATGACCCA CGCCAGAGCA ATAGAACTCA TCAAGTCGGG AGGAAGAAGA GTGCGGTTGC 
     4681 TGCTGAAACG AGGCACGGGG CAGGTCCCAG AGTATGGAAT GGTGCCTTCC AGCCTCTCCA 
     4741 TGTGCATGAA AAGTGACAAG CATGGGTCCC CATATTTCTA CTTACTGGGC CACCCTAAAG 
     4801 ACACGACGAA CCCTACGCCT GGAGCGCTGC CGCTGCCGCC GCCCCAGGCC TGCCGGAAGA 
     4861 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4921 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4981 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     5041 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5101 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5161 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5221 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5281 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5341 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5401 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5461 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5521 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5581 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5641 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5701 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5761 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5821 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5881 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5941 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     6001 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6061 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6121 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6181 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6241 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6301 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6361 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6421 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6481 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6541 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6601 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6661 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6721 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6781 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6841 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6901 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6961 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     7021 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7081 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7141 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7201 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7261 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7321 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7381 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7441 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7501 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7561 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7621 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7681 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7741 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7801 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7861 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7921 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7981 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     8041 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8101 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8161 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8221 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8281 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8341 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8401 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8461 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8521 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8581 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8641 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8701 AACGCGAATT TT
//