LOCUS       RR205234                7029 bp    DNA     circular  SYN 
DEFINITION  Dnm1l (Myc-DDK-tagged ORF) - Rat dynamin 1-like (Dnm1l), (10 ug)
ACCESSION   NM_053655
VERSION     NM_053655.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3186..3277 
                     /label="Myc-DDK"  
     misc_feature    3307..3892 
                     /label="PolyA signal"  
     misc_feature    3364..3383 
                     /label="Restriction Enzyme"  
     misc_feature    4168..4840 
                     /label="ColE1"  
     misc_feature    5270..6064 
                     /label="Kan/Neo"  
     misc_feature    6099..6401 
                     /label="SV40 ori"  
     misc_feature    6487..6964 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3168..3174 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3168 
                     /label="Dnm1l"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCGCTG ATCCCGGTCA TCAATAAGCT GCAAGACGTC TTCAACACCG 
     1081 TGGGCGCCGA CATCATCCAG CTGCCTCAGA TTGTCGTAGT GGGAACTCAG AGCAGTGGAA 
     1141 AGAGCTCAGT GCTGGAAAGC CTAGTGGGGA GGGACCTTCT TCCCAGAGGG ACTGGTGTGG 
     1201 TCACCCGGAG ACCTCTTATT CTGCAGCTGG TCCACGTTTC ACCAGAAGAT AAAAGAAAAA 
     1261 CAACAGGAGA AGAAAATGGA GTTGAAGCAG AAGAATGGGG TAAATTTCTT CACACCAAAA 
     1321 ACAAGCTTTA CACAGATTTT GATGAAATTC GACAAGAAAT TGAAAATGAA ACTGAAAGAA 
     1381 TTTCAGGAAA TAATAAGGGA GTAAGCCCTG AGCCAATCCA TCTCAAGGTT TTCTCGCCCA 
     1441 ACGTTGTCAA CCTGACGCTT GTGGATTTAC CAGGAATGAC CAAAGTACCT GTAGGTGATC 
     1501 AGCCCAAGGA TATTGAGCTT CAAATCAGAG AACTTATTCT TCGGTTCATC AGTAATCCCA 
     1561 ATTCCATTAT CCTTGCTGTC ACTGCTGCAA ATACAGATAT GGCAACGTCA GAGGCACTTA 
     1621 AGATTTCCAG AGAGGTAGAT CCAGATGGGC GCAGAACTCT AGCTGTAATC ACCAAGCTTG 
     1681 ATCTCATGGA TGCGGGTACT GATGCCATGG ATGTATTGAT GGGAAGGGTT ATTCCAGTCA 
     1741 AGCTTGGAAT AATTGGAGTA GTTAACAGGA GCCAACTGGA CATTAACAAT AAGAAGAGTG 
     1801 TAACTGATTC AATCCGTGAT GAGTATGCTT TTCTTCAAAA GAAGTACCCA TCTCTGGCCA 
     1861 ACAGAAATGG AACAAAGTAT CTTGCTAGGA CTCTGAATAG GTTACTTATG CATCATATCA 
     1921 GAGACTGTTT ACCAGAGCTG AAAACAAGAA TAAATGTCTT AGCTGCTCAG TATCAGTCTC 
     1981 TTCTAAATAG CTATGGTGAG CCCGTGGATG ATAAAAGTGC TACTTTACTC CAGCTCATTA 
     2041 CCAAGTTTGC CACAGAGTAT TGTAACACTA TTGAAGGAAC GGCAAAGTAC ATTGAAACTT 
     2101 CTGAGCTATG TGGTGGTGCT AGGATTTGTT ATATTTTCCA TGAGACTTTT GGGCGAACCT 
     2161 TAGAATCTGT TGATCCACTA GGTGGCCTTA ACACTATTGA CATCTTGACC GCCATTAGAA 
     2221 ATGCTACTGG CCCCCGTCCA GCTTTATTTG TGCCTGAAGT TTCATTTGAG TTACTGGTCA 
     2281 AACGTCAGAT TAAGCGTCTA GAGGAGCCCA GCCTCCGCTG TGTGGAGCTG GTCCACGAGG 
     2341 AAATGCAGAG GATCATTCAG CACTGTAGCA ACTACAGCAC ACAGGAACTG TTACGGTTCC 
     2401 CTAAACTCCA TGATGCCATA GTTGAAGTAG TGACTTGTCT TCTTCGTAAA AGGTTGCCCG 
     2461 TGACAAATGA AATGGTACAT AACTTAGTGG CAATTGAGCT AGCATATATC AACACAAAAC 
     2521 ACCCCGACTT TGCTGATGCC TGTGGGCTAA TGAACAATAA CATAGAGGAA CAAAGAAGAA 
     2581 ACAGACTAGC AAGAGAACTA CCTTCCGCTG TATCGCGAGA CAAGTTAATT CAGGACAACA 
     2641 GAAGAGAAAC TAAAAATGTT GCATCTGCAG GTGGTGGAAT TGGAGATGGT GGTCGAATTG 
     2701 GAGATGGTGG TCAGGAACCG ACAACAGGCA ACTGGAGAGG AATGCTGAAA ACTTCAAAAG 
     2761 CTGAAGAATT ACTTGCTGAA GAAAAATCAA AACCAATTCC AATTATGCCA GCAAGTCCAC 
     2821 AAAAAGGCCA TGCTGTCAAT TTGCTAGATG TGCCAGTTCC AGTTGCACGA AAACTGTCTG 
     2881 CCCGAGAACA GCGAGATTGT GAGGTTATTG AAAGACTCAT CAAATCATAT TTTCTAATTG 
     2941 TCAGAAAGAA TATTCAAGAC AGCGTCCCAA AGGCAGTAAT GCATTTTTTG GTTAATCATG 
     3001 TGAAAGATAC TCTTCAGAGT GAATTAGTAG GGCAGCTGTA TAAGTCATCC TTATTAGATG 
     3061 ACCTTCTGAC TGAATCCGAG GACATGGCAC AGCGAAGAAA AGAAGCGGCT GACATGCTGA 
     3121 AGGCATTACA AGGAGCCAGT CAAATTATTG CTGAAATCCG AGAGACTCAT CTTTGGACGC 
     3181 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     3241 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3301 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3361 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     3421 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     3481 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     3541 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     3601 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     3661 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     3721 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     3781 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     3841 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     3901 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     3961 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4021 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4081 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4141 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4201 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     4261 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     4321 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     4381 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     4441 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     4501 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     4561 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     4621 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     4681 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     4741 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     4801 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     4861 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     4921 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     4981 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5041 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5101 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5161 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     5221 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     5281 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     5341 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     5401 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     5461 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     5521 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     5581 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     5641 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     5701 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     5761 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     5821 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     5881 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     5941 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6001 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6061 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6121 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6181 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     6241 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     6301 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     6361 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6421 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     6481 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     6541 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     6601 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     6661 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     6721 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     6781 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     6841 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     6901 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     6961 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7021 GCGAATTTT
//