LOCUS       RR205226                8529 bp    DNA     circular  SYN 
DEFINITION  Prex2 (Myc-DDK-tagged ORF) - Rat DEP domain containing 2 (Depdc2), (10 ug)
ACCESSION   NM_001107899
VERSION     NM_001107899.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4686..4777 
                     /label="Myc-DDK"  
     misc_feature    4807..5392 
                     /label="PolyA signal"  
     misc_feature    4864..4883 
                     /label="Restriction Enzyme"  
     misc_feature    5668..6340 
                     /label="ColE1"  
     misc_feature    6770..7564 
                     /label="Kan/Neo"  
     misc_feature    7599..7901 
                     /label="SV40 ori"  
     misc_feature    7987..8464 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4668..4674 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4668 
                     /label="Prex2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGTGGAGTC CTAATGAAAA TTTCTTCCGG AAATATTCAA GAAAGGGTGT 
     1081 TTTTTCTTTT TGATAATCTT TTGGTATACT GCAAAAGAAA ACACAGACGG CTGAAGAATA 
     1141 GCAAGGCATC CACGGATGGG CATCGGTATG TCTTCCGAGG GCGGATCAAT ACCGAGGTGA 
     1201 TGGAAGTGGA GAATGTGGAT GACGGTACAG CTGATTTTCA CAGCAGTGGG CACATAGTTG 
     1261 TGAATGGGTG GAAGATACAC AACACAGCGA AAAATAAATG GTTCGTATGC ATGGCTAAAA 
     1321 GCCCTGAAGA AAAGCATGAA TGGTTTGAAG CTATCTTGAA AGAAAGAGAA CGTCGGAAAG 
     1381 GTTTAAAATT AGGAATGGAG CAAGATACCT GGGTAATGAT CTCCGAGCAG GGTGAGAAAT 
     1441 TGTATAAAAT GATGTGCAAG CAGGGAAATC TGATCAAAGA CAGAAAAAGA AAACTGACCA 
     1501 CATTCCCCAA GTGCTTCCTG GGAAGTGAAT TTGTCTCATG GCTGCTGGAA ATTGGAGAAA 
     1561 TCCACAGGCC TGAAGAGGGT GTTCACTTGG GACAAGCATT GCTAGAGAAC GGGATCATCC 
     1621 ACCATGTTAC CGATAAGCAC CAGTTCAAGC CAGAGCAAAT GCTATACCGA TTCCGATATG 
     1681 ATGATGGTAC CTTTTACCCC AGGAGTGAGA TGCAGGATGT GATTTCTAAG GGTGTAAGAC 
     1741 TCTACTGTCG TCTCCATAGT CTGTTTACTC CGGTGGTCAG AGATAAAGAT TATCATTTAA 
     1801 GAACTTACAA ATCTGTCGTC ATGGCCAACA AATTGATAGA CTGGTTAATT GCACAGGGTG 
     1861 ATTGCCGTAC CAGGGAAGAG GCAATGATAT TTGCTGTTGG GCTTTGTGAC AATGGATTCA 
     1921 TGCATCACGT CCTAGAAAAA AGTGAATTCA AAGACGAGCC TCTACTTTTC CGCTTTTTTG 
     1981 CCGATGAGGA AATGGAAGGA TCAAATATGA AACACAGGCT GATGAAACAT GACTTGAAGG 
     2041 TGGTGGAGAA TGTCATAGCT AAGTCACTGT TGATTAAATC CAATGAAGGC AGCTATGGAT 
     2101 TTGGGCTGGA AGACAAAAAT AAAGTTCCAA TAATAAAGTT AGTGGAAAAA GGGTCGAATG 
     2161 CTGAGATGGC TGGCATGGAA GTTGGGAAGA AGATATTTGC TATTAATGGT GACCTGGTTT 
     2221 TTCTGAGACC TTTTCCTGAA GTTGATTGCT TCCTGAAATC GTGCTTGAAC AGCAGAAAGC 
     2281 CACTGAGAGT CCTTGTGAGC ACAAAGCCAA GGGAGACCGT TAAGATCCCA GATTCTGCAG 
     2341 ATGGGCTTGG TTTCCAGATC AGAGGCTTTG GTCCTTCAGT TGTACATGCT GTGGGAAGAG 
     2401 GTACTGTGGC TGCAGCAGCT GGCCTTCATC CAGGACAGTG TATTATCAAA GTAAATGGAA 
     2461 TCAACGTCAG CAAGGAGACA CATGCCAGTG TCATTGCACA TGTCACCGCC TGCCGCAAAT 
     2521 ACAAGCGGCC CATGAAGCAA GATTCCATAC AGTGGGTCTA TGATAGTCTT GAAAGTGCTC 
     2581 AGGAAGACAT TCAGAAGTCC CACTCAAAGC CCCCTGGAGA CGAGGCTGGG GATGTTTTTG 
     2641 ACTGCAAAGT AGAAGATGTC ATGGACAAGT TCAACACCTT GGCCATTATC GACGGCAAGA 
     2701 AGGAGCATGT GAGTCTGACG GTGGACAATG TCCACCTCGA GTACGGGGTT GTGTACGAGT 
     2761 ATGACAGCAC GGCTGGCACA AAGTGCAATG TGGTGGAGAA GATGGTGGAG CCCAAAGGGT 
     2821 TCTTCAGCCT AACTGCCAAG ATTCTGGAAG CCCTAGCAAA AAGTGATGAA CATTTTGTCC 
     2881 AAAACTGCAC CAGCCTCAAT TCTTTGAATG AAGTGATCGC CACTGACCTT CAGAGTAAAT 
     2941 TCACCAGCAT GTGCAGTGAG AGAGTCGAGC ATGTGTGCCA CAGAATATCC AGCTATGGGA 
     3001 GGTTCTCTCG GGTGCTGAAG AACAGAGCCT GGCCCACCTT TAAGCAGGCC AAAGCTAAGA 
     3061 TGTCCCCGCT GCACAGCAGT GACTTCTGCC CTACCAACTG CCATGTGAAT GTGATGGAAG 
     3121 TTTCTTATCC GAAAGCATCG GCTTCCCTGG GCAGCGCCTT CGGTGTGCAG CTCGATAGTA 
     3181 GGAAACACAA TTCCCATGAT AAGGAAAATA GGTCTGCAGA CCCAGGGAAA CTGAGCCCCA 
     3241 TGGTATACAT TCAACACACA ATCACAACCA TGGCAGCCCC TTCAGGCCTG TCGCTGGGAC 
     3301 ACAAGGATGG CCATGGCCTC CGGTATTTGC TGAAAGAAGA AGACTTAGAA ACTCAAGACA 
     3361 TCTATCACAA ACTCCTGGGC AAGCTGCAGA CTGCACTGAA GGAGGTGGAG ATGTGCGTTT 
     3421 GTCAAATCGA TGACCTTCTG TCTTCAATAA CATATTCTCC TAAATTGGAG CGTAAGACAA 
     3481 CAGAGTATGT GATACCAACG GACAGCGACA ATGAAAAGGG AGAGAGAAAT AGCAAACGTG 
     3541 TCTGTTTTAA TGTAGCAGGA GATGAACAAG AGGATTCAGG TCATGATACA GTCAGCAACA 
     3601 GAGACTCTTA CAGTGACTGC AACAGCAACA GGAATTCCAT TGCCTCCTTC ACGAGTATCT 
     3661 GCAGCAGCCA GTGCAGTTCC TACTTTCACA GTGATGAGAT GGACTCAGGT GATGAGCTTC 
     3721 CAACCAGCAT CCGGATATCT CATGATAAAC AGGACAAGAT CCACACTTGC CTGGAGCAGC 
     3781 TCTTCATCCA GTTGGATTCA ATAACCAACC TCTTGAAAGG ACGAGCTGTT GTAAGGGCCT 
     3841 TTGAGCAAAC CAAGTATCTC ACACCAGGTC GAGGATTACA AGATAGTGAA ACCCAGCTCC 
     3901 GGAGAGACAT GGTTTTCTGC CAAAGTCTTG TGGCCACTGT CTGTGCCTTC TCTGAGCAGC 
     3961 TCATGGCTGC CTTAAACCAG ATGTTTGATA ATAGCAAGGA AAATGAAATG GAGACTTGGG 
     4021 AAGCCAGCAG GAGGTGGCTG GACCAGATTG CAAATGCGGG TGTGCTTTTT CACTTCCAGT 
     4081 CACTTCTGTC ACCAAATTTG AAAGATGAGC AAGCCATGCT TGAGGATACA CTGGTTGCCC 
     4141 TGTTTGATTT GGAAAAAGTT TCCTTTTTCT TCAAACCATC AGAAGAGGAA CCACTTGTTG 
     4201 CAAATGTGCC TCTTACATAC CAAGTGGAAG GAAGCCGGCA AGCTCTGAAA GTTGTCTTCT 
     4261 ACATGGATAG TTACCATTTT GAGCAGCTGC CCCAACGGCT GAAGAATGGA GGCGGGTTTA 
     4321 AAATTCACCC TGTTCTCTTT TCACAAGCAC TGGAGAGCAT GGAAGGATAT TGCTATAGAG 
     4381 ACAACATCTC CGTGGAAGAG TTTCAAGCGC AGATAAACAC AGCCTCCCTG GAAAAGGTCA 
     4441 AACAGTACAA TCAGAAGCTC AGGTGTACCC TGAGTGTGAC ACTGGAGCAA GCCATCACTC 
     4501 TAGCAAGAAG CCACGGGCTC CCTCCTAGGT ACATTATGCA GGCCACAGAT GTGATGAGAA 
     4561 AGCAGGGTGC AAGAGTTCAG AACACAGCTA AGAACTTGGG GGTTAGAGAC CGGACTCCTC 
     4621 AGTCAGCTCC GAGGTTGTAT AAGCTGTGTG AGCCACCACC TCCAGTGGGA GACGAAACGC 
     4681 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4741 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4801 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4861 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4921 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4981 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5041 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5101 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5161 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5221 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5281 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5341 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5401 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5461 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5521 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5581 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5641 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5701 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5761 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5821 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5881 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5941 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6001 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6061 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6121 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6181 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6241 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6301 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6361 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6421 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6481 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6541 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6601 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6661 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6721 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6781 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6841 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6901 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6961 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7021 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7081 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7141 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7201 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7261 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7321 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7381 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7441 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7501 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7561 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7621 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7681 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7741 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7801 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7861 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7921 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7981 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8041 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8101 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8161 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8221 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8281 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8341 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8401 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8461 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8521 GCGAATTTT
//