LOCUS       RR205165                8361 bp    DNA     circular  SYN 
DEFINITION  Itgal (Myc-DDK-tagged ORF) - Rat integrin alpha L (Itgal), (10 ug)
ACCESSION   NM_001033998
VERSION     NM_001033998.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4518..4609 
                     /label="Myc-DDK"  
     misc_feature    4639..5224 
                     /label="PolyA signal"  
     misc_feature    4696..4715 
                     /label="Restriction Enzyme"  
     misc_feature    5500..6172 
                     /label="ColE1"  
     misc_feature    6602..7396 
                     /label="Kan/Neo"  
     misc_feature    7431..7733 
                     /label="SV40 ori"  
     misc_feature    7819..8296 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4500..4506 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4500 
                     /label="Itgal"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTTTCCGG ATCCCTGGCC CCAGACTTCT GCTATTGGGG CTCCAGCTGT 
     1081 TTGTGAATGC CTGGAGCTAC AACCTGGACA CGCGGCATGC GCAGAGCTTC TTGACACAAG 
     1141 CTGGAAGACA TTTTGGGTAC CAGGTCTTGC AGTTTGGAGA TGGGGTTGTC GTGGGAGCTC 
     1201 CAGGAGAGGG GAACAGCACA GGAAACCTCT ATCACTGTCA CCCAAGCAGC GGCTCCTGCC 
     1261 AGCCAGTCCA CCTAGGTTCT AACCATACCT CCAAATACTT GGGAATGACC ATGGTGACAG 
     1321 ATGCTGCCAA GGGAAGCCTT TTGGCCTGTG ATCCTGGACT GTCTCGGACC TGTGATCAGA 
     1381 ACCCCTACCT CAGTGGGCTG TGCTACTTAT TTTCCCAGAG CCTGGGGAAA CCTATATTAC 
     1441 AAAATCGTCC TGCTTATCAG GAATGTATGA AGGGCAATGT CGACCTAGTG TTTCTGTTCG 
     1501 ATGGCTCACA GAGCTTGGAT AAAAAGGACT TTGAAAAAAT CGTGGACTTC ATGAAGGATG 
     1561 TGATGAGGAA ACTCAGCAAC ACTTCCTACC AGTTTGCTGC TGTTCAGTTT TCCACAGAGT 
     1621 GCAAAACAGA ATTTACTTTC TTGGACTACA TCAAGCTAAA CAAGAATCCT GATGTTCTGC 
     1681 TAGGCAACGT GACGCCCATG TTCTTGCTGA CCAATACCTT CCGTGCCATC AACTACGTGG 
     1741 TGACACACGT GTTCAAAGAA GAGTCTGGTG CCAGGCCAGA TGCTACCAAG GTGCTTGTCA 
     1801 TCATTACAGA CGGGGAAGCA AGTGACAATG GCAACATCGA TGCAGCCCAA GACATAACCC 
     1861 GCTATATCAT CGGGATTGGC AAGCATTTTT CAACCACACA AAAGCAGGAG AAACTCCACA 
     1921 TATTTGCCTC AAAACCTGTA GAGGAATTTG TCAAGATTCT GGACACATTT GAGAAGCTGA 
     1981 AGGATCTGTT TACTGATCTG CAGAGGAAGA TTTATGCCAT TGAGGGCACA AGCAAACAGG 
     2041 ACCTGGCATC CTTTAATATG GAACTCTCCT CCAGCGGGAT CAGCGCAGAC CTCAGCAAGG 
     2101 GCCATGCGGT TGTTGGAGCC GTTGGGGCTA AGGATTGGGC TGGGGGCTTT CTGGACCTGC 
     2161 ATGAAGACCT GGAGGGTGCC ACATTTGTTG GGCAGGAACC GCTGACCTCA GATGAGAGAG 
     2221 GAGGTTATCT GGGTTACACT GTGGCCTGGC TGACCTCCCG GAGCTCTAGA CCACTGCTGG 
     2281 CAGCAGGAGC TCCGCGGTAC CAGCATGTGG GACAAGTGCT GCTTTTCCAA GCCCCAGAAG 
     2341 CTGGAGGACA TTGGAACCAG ACTCAGAAGA TAGAAGGGAC TCAGATCGGA TCTTACTTTG 
     2401 GTGGGGAGCT ATGTAGCGTT GACCTGCACC AAGATGGCGA GACAGATCTG CTGCTGATTG 
     2461 GAGCGCCCCT CTTCTACGGG GAGCAGAGAG GAGGCCGAGT GTCCGTTTAC CAGAGAAGAC 
     2521 GGTCACTGTT CGAAATGGTC TCAGAGCTGC AGGGTGACCC AGGCTACCCG CTTGGTCGGT 
     2581 TTGGAGCTGC CATAAGTGCC CTGACAGACA TCAATGGGGA TGGGCTGACT GATGTGGCCG 
     2641 TGGGAGCCCC TCTGGAGGAG CAGGGGGCTG TGTATATCTT CAATGGGAAG CCTGGTGGGT 
     2701 TCAGTTCCCA GTCAAGTCAG CGTATACCAG GAACCCAGGT GTCCCCGGGA GTCCGGTGGT 
     2761 TTGGACGCTC CATCCATGGG GTGAAGGACC TTGGAGGGGA CAGGCTGGCA GATGTGGTTG 
     2821 TAGGAGCTGA GGGCCAGGTG ATTGTGCTGA GCTCGAGGCC GGTGGTGGAT ATTGTCACTG 
     2881 AGCTGTCCTT CTCCCCAGAC GAAATCCCAG TGCATGAGGT GGAGTGCTCC TACTCAGCCA 
     2941 GTCAGGAGCA GAAGGAAGGC GTCAAGCTCA AGGTGTGCTT CCAGATCCGC CCCCTTACGT 
     3001 CTCAGTTTCA AGGTCGCCTG CTTGCCAACC TCAGCTACAC CCTGCAGCTG GACGGCCATC 
     3061 GGACGAGGAG CCGAGGTCTG TTCCCAGGAG GAAGCCGTGA GCTCGGCGGA AACACATTTG 
     3121 TCACCCCAGA TAGGTCCTGC ATGGACTTCC ACTTCCACTT CCCAATCTGC ATTCAAGACC 
     3181 TCATCTCCCC TATCAATGTC TCTCTGAATT TCTCTCTTTT GGAGGAAGAA GGATCACCAA 
     3241 GGGACCAAAA GGGGGGCAGG GACATGCAGC CCATCCTGAG ACCTTCGATC CATGCAGTCA 
     3301 CTAAGGAGAT CCCCTTTGAG AAGAACTGTG GTGAGGATAA GAAGTGTGAG GCAGACCTGG 
     3361 CCCTGTCACC TCCTGCCAGA TCCGGAGTCC TGCGTCTGAT GTCCTCTGCC AGCCTTGCTG 
     3421 TGGAGTGGAC ACTGAGGAAC TTGGGAGAAG ACGCTTACTG GGTGCGACTG GACCTGGACT 
     3481 TCCCCCGGGG CCTCTCCTTC CGAAAAGTGG AGATGCTTCA GCCACACAGC CAAATACCTG 
     3541 TGAGCTGCGA GGAGCTCACC GAGGAGTCGA GTCTCCTGAC TAAGACCCTG AAATGCAATG 
     3601 TGAGCTCTCC CATCTTCAAA GCAGGCAAAC AGATGACCCT CCAGGTGATG TTTAACACGC 
     3661 TACTCAACAG TTCCTGGGGA GATTTTGTCG AGCTGAACGG CACTGTGCAC TGTGAGAACG 
     3721 AGAACTCAAG CCTCGGGAAG GACAACTCAG CCACCACCCG CATTCCTGTC CTGTACCCTG 
     3781 TCAACATCCT TACTGAGGAC CAGGAGAACT CCACACTCTA TATCAGTTTC ACCCCTAAAG 
     3841 GTCCCAAGAC TCAACAAGTC CAGCATATCT ACAAGGTGAG GATTCAGCCT TCTGCCTATG 
     3901 ACCACAACAT GCCCGCACTG GAGGCCTTGG TTGAGGTGCC GCAGCCTCAC AGCGAGGGCC 
     3961 CCATCGCACA CACATGGACT GTGCATACGG ATCCCCTTGT CACTTGCCAC TGTGAGGACC 
     4021 TGAAGAAGCC AACCAGGGAA GCTGAGCCCT GTCTGCCTGG AGTCCAGTTC CGCTGCCCAA 
     4081 TTGTCTTCAG GCAAGAGACC TTCATCCGAG TGGCCGGGAC TGTGGAACTC TCAGAGAAAA 
     4141 TCAAGGCCTC CTCCACACTC AGCCTCTGCA GCTCGCTCTC AGTCTCCTTC AACAGCAGCA 
     4201 AGCATTTCCA TTTGTATGGT AGCAAGGCCT CCAAGGCCCA GGTCCTCATG AAGGTTGACA 
     4261 TGATCTATGA GAAGGAGATG CTTCACCTGT ACGTGCTCAG CGGCATTGGG GGGCTATTGC 
     4321 TACTGTCCCT GATTTTCCTA GCGCTCTACA AGGTTGGTTT CTTCAAACGG AAGCTGAAGG 
     4381 AGAAGATGGA GGCGGATGGA GGTGTTCCAA ATGGAAGCCC TGGCGAAGAC GCTGACCCTC 
     4441 TGGCAGTACC TGGGGAAGAG ACCAAAGATA TGGGCTGTCT AGAGCCCCTC CGTGAGAGTG 
     4501 ACAAGGACAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4561 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4621 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4681 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4741 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4801 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4861 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4921 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4981 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5041 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5101 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5161 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5221 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5281 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5341 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5401 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5461 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5521 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5581 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5641 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5701 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5761 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5821 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5881 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5941 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6001 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6061 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6121 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6181 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6241 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6301 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6361 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6421 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6481 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6541 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6601 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6661 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6721 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6781 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6841 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6901 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6961 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7021 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7081 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7141 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7201 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7261 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7321 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7381 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7441 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7501 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7561 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7621 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7681 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7741 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7801 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7861 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7921 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7981 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8041 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8101 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8161 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8221 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8281 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8341 CAAAAATTTA ACGCGAATTT T
//