LOCUS       RR205074                8346 bp    DNA     circular  SYN 
DEFINITION  Cacna2d2 (Myc-DDK-tagged ORF) - Rat calcium channel, voltage-dependent, alpha 2/delta subunit 2 (Cacna2d2), (10 ug)
ACCESSION   NM_175592
VERSION     NM_175592.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4501..4592 
                     /label="Myc-DDK"  
     misc_feature    4622..5207 
                     /label="PolyA signal"  
     misc_feature    4679..4698 
                     /label="Restriction Enzyme"  
     misc_feature    5483..6155 
                     /label="ColE1"  
     misc_feature    6585..7379 
                     /label="Kan/Neo"  
     misc_feature    7414..7716 
                     /label="SV40 ori"  
     misc_feature    7802..8279 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4491..4499 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4491 
                     /label="Cacna2d2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGTGCCG GCTCGGACCT GCGGCGCTTC TTGGCCCGGC CCGGTGCGGA 
     1081 CCGCTCGCCC CTGGCCCGGT CGCGGTCCCC GGCCCTGCCC TGACCCCCGG GGCCCAGCGT 
     1141 CCGGGCCCGC ACGCCCGCTC TTGCTACTGC TGCCGCCTCT GCTGCTTTTA CCGCTGCTCA 
     1201 CCGCCCCCGG CGCCTCTGCC TACAGCTTCC CCCAGCAGCA CACGATGCAG CACTGGGCCC 
     1261 GGCGCCTGGA GCAGGAGATT GACGGTGTGA TGCGGATTTT TGGAGGCGTG CAGCAGCTCC 
     1321 GAGAGATCTA CAAGGACAAT CGGAACCTGT TTGATGTGCA GGAGAATGAA CCACAGAAAC 
     1381 TAGTGGAGAA GGTGGCAGGG GACATTGAGA GCCTGCTGGA CAGAAAGGTC CAGGCCTTGA 
     1441 AGAGACTGGC TGACGCTGCA GAGAATTTCC AGAAAGCCCA CCGCTGGCAA GACAACATCA 
     1501 AGGAGGAAGA CATCATGTAC TATGACGCCA AGGCTGACGC CGAGCTGGAT GATCCTGAGA 
     1561 GTGAGGATAT GGAGAGGGGA TCCAAGACCA GCGCCTTAAG GCTGGACTTC ATCGAGGAGC 
     1621 CAAACTTCAA GAACAAAGTC AACTATTCAT ACACGGCGGT GCAGATCCCC ACAGATATCT 
     1681 ACAAAGGCTC TACCGTCATC CTCAATGAGC TTAACTGGAC GGAGGCCCTG GAGAATGTCT 
     1741 TCATTGAGAA CCGTAGGCAA GACCCTACAC TGTTGTGGCA AGTCTTCGGC AGTGCCACGG 
     1801 GAGTCACCCG CTATTACCCA GCCACACCGT GGCGAGCCCC CAAGAAGATT GACCTGTACG 
     1861 ATGTCAGAAG ACGACCCTGG TATATACAGG GGGCCTCATC ACCCAAGGAC ATGGTCATCA 
     1921 TTGTGGATGT GAGTGGCAGT GTGAGCGGCC TGACGCTGAA GCTGATGAAG ACGTCTGTCT 
     1981 GTGAGATGCT AGACACACTC TCCGATGATG ACTATGTGAA TGTGGCCTCA TTCAACGAGA 
     2041 AGGCGCAGCC TGTGTCTTGC TTCACACACC TGGTGCAGGC CAATGTGCGG AACAAGAAGG 
     2101 TGTTCAAGGA AGCTGTGCAG GGCATGGTGG CCAAGGGCAC CACAGGCTAC AAGGCTGGCT 
     2161 TTGAGTATGC CTTTGACCAG CTACAGAATT CCAACATCAC CCGTGCTAAC TGCAATAAGA 
     2221 TGATCATGAT GTTCACGGAC GGGGGAGAGG ATCGCGTGCA GGACGTCTTT GAAAAGTATA 
     2281 ATTGGCCCAA TCGGACGGTA CGCGTCTTCA CGTTCTCCGT AGGACAGCAT AACTATGATG 
     2341 TCACACCCCT GCAGTGGATG GCTTGTACTA ACAAAGGTTA CTATTTTGAG ATCCCTTCCA 
     2401 TCGGAGCCAT CCGCATCAAC ACACAGGAAT ACCTGGATGT GCTGGGTAGG CCCATGGTCC 
     2461 TGGCAGGCAA GGACGCCAAG CAAGTGCAAT GGACAAACGT GTATGAAGAT GCGCTGGGGC 
     2521 TGGGGTTGGT GGTAACAGGG ACTCTCCCTG TTTTCAACCT GACACAGGAT GGCCCTGGGG 
     2581 ACAAGAAGAA CCAGCTAATC CTGGGTGTCA TGGGCATAGA TGTGGCCCTG AATGACATCA 
     2641 AAAGGCTGAC TCCCAACTAC ACACTTGGCG CCAATGGCTA CGTGTTCGCC ATCGACCTGA 
     2701 ATGGCTATGT GTTGCTACAT CCCAATCTCA AGCCCCAGAT TACCAACTTC CGGGAGCCTG 
     2761 TGACCTTGGA CTTCCTGGAT GCAGAGCTGG AAGATGAGAA CAAGGAGGAG ATCCGTCGTA 
     2821 GTATGATTGA CGGAGACAAA GGCCACAAGC AGATCAGAAC CTTGGTCAAA TCCCTGGATG 
     2881 AGAGGTACAT AGATGAAGTG ATTCGGAACT ACACCTGGGT GCCTATAAGG AGTACCAACT 
     2941 ACAGCCTGGG GCTGGTGCTC CCACCCTACA GCACCTACTA CCTCCAAGCC AACCTCAGCG 
     3001 ACCAGATCCT GCAGGTCAAG TTGCCAATCA GCAAACTGAA GGATTTTGAG TTCCTGCTCC 
     3061 CCAGCAGCTT TGAGTCTGAA GGACATGTTT TCATTGCTCC CAGAGAGTAT TGCAAGGATT 
     3121 TGAATGCCTC AGACAACAAC ACCGAGTTCC TGAAAAACTT CATTGAGCTC ATGGAGAAAG 
     3181 TGACTCCGGA CTCCAAGCAG TGCAATAACT TCCTTCTGCA TAACTTGATT TTGGACACGG 
     3241 GCATTACGCA GCAGTTAGTG GAACGCGTCT GGCGGGACCA AGATCTCAAC ACGTACAGCC 
     3301 TGCTAGCTGT ATTTGCTGCC ACTGACGGTG GCATCACACG TGTCTTCCCG AACAAGGCAG 
     3361 CTGAAGACTG GACAGAAAAC CCTGAACCCT TCAATGCCAG CTTCTACCGC CGCAGCCTGG 
     3421 ATAACCGTGG TTATATCTTC AAGCCCCCGC ACCAGGACTC CCTGTTAAGG CCACTGGAGC 
     3481 TGGAGAATGA CACAGTAGGC GTCCTCGTCA GCACAGCTGT AGAGCTCAGT CTGGGTCGCC 
     3541 GCACACTGAG GCCAGCAGTG GTGGGTGTCA AACTGGACCT AGAGGCTTGG GCTGAAAAGT 
     3601 TCAAGGTGCT TGCCAGCAAC CGTACCCATC AGGACCAACC TCAGAAGCAG TGCGGCCCCA 
     3661 GCAGCCACTG TGAGATGGAC TGCGAGGTAA ACAACGAGGA CCTACTCTGT GTCCTCATTG 
     3721 ATGACGGGGG ATTCCTGGTG CTGTCAAACC AAAACCACCA GTGGGACCAG GTTGGCAGGT 
     3781 TCTTCAGTGA GGTGGATGCC AACCTGATGC TGGCACTGTA CAATAACTCC TTCTACACCA 
     3841 GAAAGGAGTC CTATGACTAT CAGGCAGCTT GTGCCCCTCA GCCTCCTGGC AACCTGGGTG 
     3901 CTGCACCCAG GGGTGTCTTT GTGCCCACCA TTGCAGATTT CCTTAACTTG GCCTGGTGGA 
     3961 CCTCTGCTGC CGCCTGGTCC TTATTCCAGC AACTACTTTA TGGTCTCATC TATCACAGCT 
     4021 GGTTCCAGGC AGACCCGGCA GAAGCCGAGG GCAGCCCCGA GACGCGCGAG AGCAGCTGCG 
     4081 TCATGAAACA AACCCAGTAC TACTTCGGCT CGGTGAACGC GTCCTATAAC GCCATCATCG 
     4141 ACTGCGGAAA CTGCAGCAGG CTGTTCCACG CGCAGAGACT GACCAACACC AACCTTCTGT 
     4201 TCGTGGTGGC GGAGAAGCCG CTGTGCAGCC AGTGCGAGGT CGGCCGGCTG CTACAAAAGG 
     4261 AGACACACTG CCCAGCGGAC GGCCCGGAGC AGTGTGAGCT GGTGCAGAGA CCGCGATACC 
     4321 GAACAGGCCC GCACATCTGT TTTGACTACA ATGCGACGGA AGATACCTCA GACTGTGGCC 
     4381 GCGGAGCCTC CTTCCCTCCG TCGCTGGGCG TCTTGGTTTC CCTGCAGCTG TTGCTCCTCC 
     4441 TAGGCCTGCC ACCTCGGCCG CAGCCTCAAA TCCATTCCTT CACGCCCTCT CGCCGCCTCA 
     4501 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4561 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4621 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4681 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4741 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4801 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4861 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4921 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4981 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5041 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5101 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5161 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5221 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5281 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5341 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5401 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5461 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5521 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5581 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5641 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5701 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5761 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5821 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5881 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5941 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6001 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6061 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6121 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6181 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6241 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6301 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6361 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6421 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6481 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6541 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6601 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6661 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6721 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6781 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6841 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6901 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6961 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7021 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7081 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7141 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7201 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7261 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7321 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7381 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7441 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7501 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7561 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7621 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7681 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7741 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7801 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7861 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7921 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7981 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8041 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8101 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8161 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8221 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8281 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8341 AATTTT
//