LOCUS       RR205061                7905 bp    DNA     circular  SYN 
DEFINITION  Trim37 (Myc-DDK-tagged ORF) - Rat tripartite motif-containing 37 (Trim37), (10 ug)
ACCESSION   NM_001108288
VERSION     NM_001108288.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4062..4153 
                     /label="Myc-DDK"  
     misc_feature    4183..4768 
                     /label="PolyA signal"  
     misc_feature    4240..4259 
                     /label="Restriction Enzyme"  
     misc_feature    5044..5716 
                     /label="ColE1"  
     misc_feature    6146..6940 
                     /label="Kan/Neo"  
     misc_feature    6975..7277 
                     /label="SV40 ori"  
     misc_feature    7363..7840 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4044..4050 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4044 
                     /label="Trim37"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGAGCAG AGTGTGGAGA GCATTGCTGA GGTTTTCCGA TGCTTCATTT 
     1081 GTATGGAGAA ATTGCGGGAT GCCCGACTGT GTCCTCACTG CTCCAAGCTC TGTTGTTTCA 
     1141 GCTGTATTAG GCGCTGGCTG ACAGAGCAGA GAGCTCAGTG TCCTCATTGT CGTGCTCCAC 
     1201 TCCAGCTACG GGAACTTGTA AATTGTCGCT GGGCGGAAGA GGTGACACAG CAGCTGGATA 
     1261 CTCTCCAGCT GTGTAGTCTC ACCAAACATG AAGAAAATGA GAAAGACAAA TGTGAAAATC 
     1321 ACCATGAAAA ACTTAGTGTG TTTTGCTGGA CTTGTAAGAA GTGTATCTGC CATCAGTGTG 
     1381 CACTTTGGGG AGGAATGCAT GGTGGACACA CGTTTAAACC TTTGGCAGAA ATCTATGAAC 
     1441 AGCATGTCAC TAAAGTGAAC GAAGAGGTAG CCAAACTTCG TCGACGTCTC ATGGAACTGA 
     1501 TCAGCTTAGT TCAAGAAGTG GAAAGAAATG TAGAAGCGGT GAGAAATGCG AAGGACGAAC 
     1561 GTGTTCGGGA AATCAGGAAC GCGGTGGAGA TGATGATTGC ACGGCTGGAC ACACAGCTGA 
     1621 AGAACAAGCT CATAACCCTC ATGGGTCAGA AGACGTCTCT TACTCAAGAA ACAGAGCTGT 
     1681 TGGAATCTTT ACTTCAGGAG GTAGAACATC AGTTGCGGTC TTGCAGTAAG AGTGAGCTGA 
     1741 TCTCCAAGAG CGCAGAGATC CTAATGATGT TCCAGCAAGT TCACCGAAAG CCCATGGCTT 
     1801 CCTTTGTTAC CACACCTGTC CCACCAGACT TTACCAGTGA ATTGGTACCA TCTTATGATT 
     1861 CAGCTACTTT TGTTTTAGAG AACTTCAGCA CTTTGCGGCA GAGAGCAGAT CCGGTTTACA 
     1921 GCCCGCCTCT TCAAGTTTCA GGACTTTGCT GGAGGTTAAA AGTTTACCCA GATGGAAATG 
     1981 GAGTTGTACG TGGCTATTAT TTATCTGTAT TTTTGGAACT ATCGGCTGGC TTACCTGAAA 
     2041 CTTCCAAGTA TGAATATCGT GTAGAAATGG TTCATCAGTC CTGCAACGAT CCTACTAAAA 
     2101 ATATCATTCG AGAATTTGCA TCTGACTTTG AAGTTGGAGA ATGCTGGGGC TACAATAGAT 
     2161 TTTTCCGTTT GGACTTACTT GCAAATGAAG GATACTTGAA CCGACAAAAT GATACAGTGA 
     2221 TTTTAAGGTT TCAGGTGCGC TCACCAACAT TTTTTCAAAA ATGCCGGGAT CAGCACTGGT 
     2281 ACATTACTCA ATTGGAAGCT GCACAGACAG GTTATATTCA ACAAATAAAC AATCTTAAAG 
     2341 AGAGATTGAC TATTGAACTG TCCCGAACTC AGAAATCCAG AGATCTGTCA CCACCAGATA 
     2401 ATCATCTTAG CCCCCAAAAT GATGATACTC CCGAGACACG AGCTAAGAAA GCTGGGTCAT 
     2461 GCTCTGACAT GCTTCTGGAA GGTGGTCCTA CTTCAGCTTC TGTAAGGGAG ACCAAGGAAG 
     2521 ATGAAGATGA GGAGGAGAAG ATTCAGAATG AAGACTATCA TCATGAGCTC TCAGATGGAG 
     2581 ATCTGGATCT GGATCTTGTC GGTGAAGATG AAGTGAATCA CCTGGACGGC AGCAGCTCTT 
     2641 CTGCTAGTTC TACAGCAACA AGCAACACGG AAGAAAACGA TATCGATGAG GAGACCATGT 
     2701 CTGGAGAGAA TGATGTAGAA TATAACAGCA TGGAGCTGGA GGAGGGAGAG CTCATGGAAG 
     2761 ACGCAGCCGC GGCGGGGCCT CCAGGCAGTA GCCACAGCTA TGTGGGTGCC AGTAGCAGAA 
     2821 TGTCAAGAAG AGCACATTTA TGCTCTGCAG CTACCAGTAG CTTATTAGAC ATTGATCCTT 
     2881 TAATCCTAAT ACATTTACTG GATCTTAAGG ACCGGAGCAG TATGGAAAAT CTGTGGGGTT 
     2941 TACAGCCTCG TCCATCTGCT TCACTTTTGC AACCCACAGC GTCATATTCT CGAAAAGATA 
     3001 AAGATCAAAG GAAGCAGCAG GCGATGTGGC GAGTGCCCTC TGACCTAAAG ATGCTGAAAA 
     3061 GGCTCAAAAC ACAAATGGCT GAAGTTCGGT GTATGAAGAC TGATGTGAAG ACTACACTGG 
     3121 CAGATATAAA GGGCAGCAGT GTTGCTTCTG CAGACTTGCA GACAAACCTT TTCTGTGCTG 
     3181 ACCAGGCAGC TCTGGCTACC TGTGGACCTG AAAACTCTGG TAGATTGCAG GATTTGGGCA 
     3241 TGGAGCTTCT GGCAAAGTCG TCAGTTGCTG GCTGTTACAT AAGAAACCCC ACAAATAAGA 
     3301 AGAATTCACC CAAGTCAGCT CGGGCCATAG CAGGCAGTCT GTCACTCCGA AGAGCTGTAG 
     3361 ACTCTGGAGA AAACAGCCGT TCAAAGGGAG ACTGTCAGGC TTTGTCTGAA GGCTCCCCAG 
     3421 GAAGCTCTCA GTCTGGGAGC CGACACAGCT CCCCCCGGGC TCTGACACAT GGCATCATTG 
     3481 GGGACCTTCT GCCCAAAAGT GAAGACCGAC AGTGCAAAGC TTTGGATTCT GATGCTGTGG 
     3541 TGGTTGCAGT TTTCAATGGT TTACCTACTG TTGAGAAAAG AAGGAAAATG GTCACTCTGG 
     3601 GGACTAATGT AAAAGGAGGT CGTCTGGAAG GAATGCAATT GGCAGATTTG GAAAGTCCTT 
     3661 CTGATGCTGG GGAGGTACAG CCCACACTAC CTGAAGGAGC CTCAGCTGCC CCTGAGGAAG 
     3721 GAATGAGTAG TGACAGCGAC ATTGAATGTG ACACTGAGAA CGAGGAGCAG GAAGAGCACA 
     3781 CCAGCATGGG TGCCTTCAAC GATCCGTTCC TGGCCCAGCC TCCGGATGAA GATTCGCATT 
     3841 CCAGTTTTCC TGATGGTGAA CAAATAGACC CTGAAAATCT CCACTTCAAC ACCGATGAAG 
     3901 GCAGTGGAAG ACTGCTTGTT CTTGGAACCC AGCAGCCATA CTGTGAGGAA GTCCAAACCA 
     3961 GCCAGCCTGG GGAGACTGCA TGTACAGGGT CACACGGGTA TGAGCTGAGA CCTCTGGCCC 
     4021 CAGCTAACAG CCACCATCAG CTGCCTGACG TGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4081 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4141 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4201 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4261 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4321 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4381 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4441 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4501 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4561 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4621 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4681 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4741 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4801 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4861 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4921 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4981 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5041 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5101 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5161 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5221 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5281 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5341 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5401 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5461 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5521 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5581 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5641 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5701 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5761 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5821 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5881 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5941 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6001 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6061 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6121 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6181 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6241 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6301 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6361 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6421 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6481 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6541 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6601 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6661 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6721 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6781 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6841 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6901 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6961 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7021 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7081 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7141 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7201 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7261 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7321 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7381 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7441 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7501 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7561 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7621 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7681 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7741 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7801 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7861 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//