LOCUS       RR205013                8421 bp    DNA     circular  SYN 
DEFINITION  Itga1 (Myc-DDK-tagged ORF) - Rat integrin alpha 1 (Itga1), (10 ug)
ACCESSION   NM_030994
VERSION     NM_030994.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4578..4669 
                     /label="Myc-DDK"  
     misc_feature    4699..5284 
                     /label="PolyA signal"  
     misc_feature    4756..4775 
                     /label="Restriction Enzyme"  
     misc_feature    5560..6232 
                     /label="ColE1"  
     misc_feature    6662..7456 
                     /label="Kan/Neo"  
     misc_feature    7491..7793 
                     /label="SV40 ori"  
     misc_feature    7879..8356 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4560..4566 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4560 
                     /label="Itga1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCCCCAGG CGTCCTGCCA GCCTAGAGGT CACTGTAGCC TGCATATGGC 
     1081 TTCTCACGGT CATCCTAGGC TTCTGCGTCT CCTTCAATGT TGATGTGAAA AACTCAATGA 
     1141 GTTTCAGTGG CCCAGTAGAG GACATGTTTG GATACACTGT TCAACAATAT GAAAACGAAG 
     1201 AAGGCAAATG GGTTCTTATT GGTTCTCCTT TAGTTGGCCA ACCCAAAGCA AGAACTGGAG 
     1261 ATGTCTATAA GTGTCCGGTT GGGAGAGAGA GAGCAATGCC TTGCGTGAAG TTGGACTTGC 
     1321 CAGTTAACAC ATCGATCCCC AATGTCACAG AAATAAAGGA AAACATGACA TTTGGATCAA 
     1381 CTTTAGTCAC CAACCCGAAT GGAGGATTTC TGGCATGTGG GCCCTTGTAT GCCTATAGAT 
     1441 GTGGACATTT GCATTATACA ACTGGAATAT GTTCTGATGT CAGTCCTACA TTTCAAGTTG 
     1501 TGAACTCCTT TGCCCCTGTA CAAGAATGCA GCACCCAGCT GGACATAGTC ATCGTCCTGG 
     1561 ATGGCTCCAA CAGCATCTAC CCCTGGGAAA GTGTCATCGC CTTTTTAAAC GACCTTCTTA 
     1621 AGAGGATGGA TATTGGCCCT AAGCAGACAC AGGTCGGGAT TGTACAGTAT GGAGAGAATG 
     1681 TAACCCATGA GTTCAACCTC AATAAGTATT CATCCACAGA AGAGGTCCTT GTCGCAGCAA 
     1741 ACAAAATAGG CCGACAGGGA GGCCTCCAAA CGATGACAGC CCTTGGAATA GACACAGCCA 
     1801 GGAAAGAGGC ATTCACTGAA GCTCGGGGTG CCAGGAGGGG AGTTAAAAAA GTCATGGTTA 
     1861 TTGTGACCGA CGGAGAATCG CATGACAACT ATCGCCTGAA ACAGGTCATC CAAGACTGCG 
     1921 AGGACGAAAA CATTCAGCGA TTTTCCATAG CTATCCTTGG CCACTATAAC AGGGGGAACT 
     1981 TAAGCACTGA AAAATTTGTG GAGGAAATAA AATCGATCGC AAGCGAGCCC ACGGAAAAGC 
     2041 ACTTCTTCAA TGTCTCGGAT GAGTTGGCCC TGGTCACTAT TGTTAAAGCT CTGGGAGAAA 
     2101 GGATATTCGC TTTGGAAGCG ACAGCTGACC AGTCAGCAGC TTCATTTGAG ATGGAAATGT 
     2161 CTCAGACTGG CTTCAGTGCT CACTACTCCC AGGACTGGGT CATGCTTGGA GCGGTGGGAG 
     2221 CCTATGACTG GAACGGAACT GTGGTCATGC AGAAGGCTAA CCAGATGGTC ATCCCTCATA 
     2281 ACACCACCTT TCAAACTGAG CCCGCCAAGA TGAACGAGCC TCTGGCTTCT TATTTAGGTT 
     2341 ACACAGTGAA CTCGGCCACC ATCCCTGGAG ATGTGCTCTA CATCGCTGGG CAGCCTCGGT 
     2401 ACAATCATAC GGGCCAGGTC GTCATCTACA AGATGGAGGA TGGGAACATC AACATTCTGC 
     2461 AGACACTCGG CGGAGAGCAG ATTGGTTCCT ACTTTGGTAG TGTCTTAACA ACAATTGACA 
     2521 TCGACAAAGA TTCTTATACT GATCTGCTTC TCGTCGGGGC CCCCATGTAC ATGGGGACAG 
     2581 AGAAAGAGGA ACAGGGCAAG GTGTACGTGT ACGCTGTGAA TCAGACAAGG TTTGAATATC 
     2641 AAATGAGCCT GGAACCAATT AGGCAGACCT GCTGCTCATC CCTGAAGGAT AATTCATGCA 
     2701 CGAAAGAAAA CAAGAATGAG CCCTGCGGGG CCCGCTTCGG AACAGCAATT GCTGCTGTAA 
     2761 AAGACCTCAA CGTGGATGGA TTTAATGACG TCGTGATTGG AGCTCCGCTG GAAGATGACC 
     2821 ACGCAGGAGC TGTGTACATT TATCATGGCA GTGGCAAGAC CATAAGGGAG GCGTATGCAC 
     2881 AACGCATTCC ATCAGGTGGG GATGGCAAGA CCCTGAAATT TTTCGGCCAG TCTATCCACG 
     2941 GAGAGATGGA TTTAAATGGT GACGGTCTGA CTGACGTGAC CATTGGAGGC CTTGGTGGAG 
     3001 CAGCCCTCTT CTGGGCCAGA GATGTGGCTG TAGTTAAAGT GACCATGAAT TTTGAACCCA 
     3061 ATAAAGTGAA TATTCAAAAG AAAAACTGCC GTGTGGAGGG CAAAGAAACA GTGTGCATAA 
     3121 ATGCTACAAT GTGTTTTCAT GTGAAATTAA AGTCTAAAGA GGACTCAATT TACGAGGCTG 
     3181 ATCTGCAGTA CCGTGTCACC CTTGATTCAC TGAGGCAGAT ATCACGGAGC TTTTTTTCTG 
     3241 GAACTCAGGA AAGGAAGATT CAAAGAAATA TCACCGTTCG AGAATCAGAA TGCATCAGGC 
     3301 ACTCCTTCTA CATGTTGGAC AAACATGACT TTCAGGACTC TGTGAGAGTG ACTCTGGATT 
     3361 TTAATCTCAC TGATCCAGAA AATGGTCCTG TACTTGATGA CGCTCTGCCA AACTCAGTCC 
     3421 ACGAACACAT TCCCTTTGCC AAAGACTGTG GAAACAAGGA AAGATGCATT TCAGACCTCA 
     3481 CTCTGAATGT GTCCACCACA GAAAAGAGCC TGCTGATCGT CAAGTCCCAG CATGACAAGT 
     3541 TCAACGTTAG CCTCACCGTC AAAAACAAAG GAGACAGTGC GTACAACACC AGGACAGTGG 
     3601 TGCAGCATTC ACCAAATCTG ATTTTTTCGG GAATTGAGGA GATCCAAAAA GATAGCTGTG 
     3661 AATCTAATCA AAATATCACT TGCAGAGTTG GATATCCTTT CCTAAGAGCA GGAGAAACGG 
     3721 TTACCTTCAA AATAATATTC CAGTTTAACA CATCCCATCT CTCGGAAAAT GCAATCATTC 
     3781 ACTTAAGTGC AACAAGTGAC AGTGAGGAGC CCCTGGAATC TCTTAATGAT AATGAAGTAA 
     3841 ATATTTCCAT CCCAGTAAAA TATGAAGTTG GACTGCAGTT TTACAGTTCT GCGAGTGAAC 
     3901 ATCACATTTC AGTCGCTGCC AATGAGACGA TCCCTGAGTT TATTAACTCC ACTGAGGACA 
     3961 TTGGGAATGA AATTAATGTC TTCTATACGA TTAGAAAGAG GGGGCATTTC CCAATGCCAG 
     4021 AACTTCAGCT GTCAATTTCA TTCCCCAATT TGACGGCAGA TGGTTATCCT GTACTGTACC 
     4081 CAATTGGATG GTCATCTTCA GATAATGTGA ACTGTAGACC CCGGAGCCTT GAGGACCCCT 
     4141 TTGGCATCAA CTCTGGGAAG AAAATGACAA TATCGAAGTC TGAGGTTCTC AAAAGAGGCA 
     4201 CAATCCAGGA CTGCAGTAGT ACGTGTGGAG TTGCCACCAT CACGTGTAGC CTCCTTCCTT 
     4261 CCGACCTGAG TCAAGTGAAT GTCTCGCTCC TCCTGTGGAA ACCGACTTTC ATAAGAGCAC 
     4321 ATTTTTCCAG CTTAAACCTT ACTCTAAGAG GAGAACTTAA GAGTGAAAAT TCATCGCTGA 
     4381 CTTTAAGTAG CAGCAACCGG AAGCGAGAGC TGGCTATTCA GATATCCAAA GACGGGCTCC 
     4441 CAGGCAGAGT GCCGCTGTGG GTTATCCTCC TGAGCGCCTT CGCGGGGCTA CTGCTGCTAA 
     4501 TGCTCCTTAT ATTGGCTCTG TGGAAGATTG GATTCTTCAA AAGGCCACTG AAGAAGAAAA 
     4561 TGGAGAAAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4621 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4681 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4741 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4801 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4861 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4921 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4981 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5041 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5101 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5161 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5221 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5281 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5341 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5401 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5461 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5521 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5581 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5641 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5701 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5761 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5821 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5881 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5941 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6001 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6061 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6121 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6181 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6241 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6301 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6361 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6421 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6481 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6541 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6601 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6661 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6721 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6781 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6841 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6901 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6961 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7021 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7081 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7141 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7201 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7261 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7321 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7381 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7441 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7501 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7561 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7621 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7681 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7741 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7801 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7861 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7921 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7981 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8041 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8101 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8161 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8221 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8281 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8341 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8401 CAAAAATTTA ACGCGAATTT T
//