LOCUS       RR205005                6431 bp    DNA     circular  SYN 
DEFINITION  Gba (Myc-DDK-tagged ORF) - Rat glucosidase, beta, acid (Gba), (10 ug)
ACCESSION   NM_001127639
VERSION     NM_001127639.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2588..2679 
                     /label="Myc-DDK"  
     misc_feature    2709..3294 
                     /label="PolyA signal"  
     misc_feature    2766..2785 
                     /label="Restriction Enzyme"  
     misc_feature    3570..4242 
                     /label="ColE1"  
     misc_feature    4672..5466 
                     /label="Kan/Neo"  
     misc_feature    5501..5803 
                     /label="SV40 ori"  
     misc_feature    5889..6366 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2570..2576 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2570 
                     /label="Gba"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGCCAGG TTCATAGGAC TCTTCCTATT TCAGGCCATA TCTTGGGCAT 
     1081 ATGGTGCCCA ACCCTGCATC CCCAAAAGTT TTGGCTACAG CTCAGTGGTC TGTGTCTGCA 
     1141 ATGCAACGTA CTGTGACTCT CTTGACCCCC TGACCTTACC GGCTCTGGGT ACCTTCAGCC 
     1201 GTTATGAGAG TACTCGAAGC GGACGTCGGA TGGAGCTGAG TACCGGGTCC ATCCAGGCCA 
     1261 ACCGCACTGG CACAGGGCTC CTACTAACCT TGCAGCCAGA AGAGAAGTTC CAGAAAGTGA 
     1321 AAGGATTTGG AGGTGCCATG ACAGATGCCA CTGCGCTCAA CATCCTTGCC TTGTCCCCCC 
     1381 CGGCTCAGAA ACTGCTACTC AAGTCCTACT TCTCTAGCGA AGGCATTGAG TATAACATCA 
     1441 TCCGGGTACC CATGGCCAGT TGTGACTTCT CCATCCGCAT CTACACCTAT GCTGACACCC 
     1501 CTAATGACTT CCAGTTATCC AACTTCAGCC TCCCGGAGGA AGACACCAAG CTCAAGATAC 
     1561 CCCTGATTCA CCGAGCCCTG AAGATGTCCC CACGCCCCAT TTCACTCTTT GCCAGTCCCT 
     1621 GGACATCACC CACTTGGCTC AAGACCAATG GAGCAGTGAA TGGGAAAGGG TCACTCAAGG 
     1681 GTCACCCAGG GGATATCTAT CACGAGGCCT GGGCCAATTA CTTTGTCAAG TTTCTGGATG 
     1741 CTTATGCTAC ACACAACATC AAATTCTGGG CAGTGACAGC GGAGAATGAA CCCTCTGCAG 
     1801 GGCTCTTCAC TGGGTACCCC TTCCAATGCC TGGGCTTCAC TGCTGAACAT CAGAGAGACT 
     1861 TTATTTCCCA TGATCTAGGG CCAGCCCTTG CCAACAGTTC TCATGATGTG AAGCTACTCA 
     1921 TACTGGATGA TCAGCGGCTG CTGCTACCCC GCTGGGCACA GGTGGTGCTC TCTGACCCAG 
     1981 AGGCAGCCAA GTACGTTCAT GGCATCGCTG TCCACTGGTA TATGGATTTC CTGGCTCCAG 
     2041 CCAAAGCTAC TTTAGGAGAG ACACACCGCT TGTTCCCCAA CATGATGCTC TTTGCTTCAG 
     2101 AGGCCTGCGT GGGCTCCAAG TTCTGGGAGC AGAGTGTTCG GTTAGGCTCC TGGGATCGAG 
     2161 GGATGCAGTA CAGTCACAGC ATCATTACGA ACCTCCTTTA CCACGTAACT GGATGGACTG 
     2221 ACTGGAACCT TGCCCTGAAT CCTGAAGGAG GGCCCAACTG GGTCCGCAAC TTCGTTGATA 
     2281 GCCCCATCAT TGTCGACATC CCCAAAGACA CATTTTACAA GCAGCCCATG TTCTACCATC 
     2341 TTGGCCACTT CAGCAAGTTC ATCCCTGAGG GATCTCAGCG AGTGGGCTTG GTGGCCAGTG 
     2401 AGAAGACCGA CTTGGAAACA GTGGCACTGA TACGCCCTGA TGGCTCTGCA GTTGTGGTCG 
     2461 TGTTAAACCG ATCGTCTAAG GATGTCCCTC TTACCATCAG TGATCCTGCC CTGGGCTTCA 
     2521 TGGAGACCAT CTCACCTGGC TACTCCATTC ACACTTACCT ATGGCGTCGC CAGGCCAGAC 
     2581 GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT 
     2641 CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT 
     2701 CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG 
     2761 AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT 
     2821 CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC 
     2881 AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG 
     2941 TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC 
     3001 AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT 
     3061 TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG 
     3121 TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT 
     3181 TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA 
     3241 GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT 
     3301 CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG 
     3361 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA 
     3421 AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG 
     3481 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG 
     3541 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC 
     3601 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG 
     3661 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT 
     3721 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC 
     3781 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC 
     3841 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG 
     3901 TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA 
     3961 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC 
     4021 GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT 
     4081 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT 
     4141 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT 
     4201 TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG 
     4261 ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA 
     4321 GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC 
     4381 TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT 
     4441 TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC 
     4501 CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG 
     4561 CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG 
     4621 AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG 
     4681 CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA 
     4741 TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC 
     4801 GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA 
     4861 ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA 
     4921 CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG 
     4981 CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG 
     5041 TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA 
     5101 GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT 
     5161 GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT 
     5221 CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC 
     5281 GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA 
     5341 CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT 
     5401 GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC 
     5461 CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG 
     5521 CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC 
     5581 TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG 
     5641 GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG 
     5701 AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT 
     5761 GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT 
     5821 CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT 
     5881 TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT 
     5941 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG 
     6001 ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG 
     6061 CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA 
     6121 CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA 
     6181 GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC 
     6241 CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG 
     6301 GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT 
     6361 AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA 
     6421 ACGCGAATTT T
//