LOCUS       RR204893                6951 bp    DNA     circular  SYN 
DEFINITION  Thop1 (Myc-DDK-tagged ORF) - Rat thimet oligopeptidase 1 (Thop1), (10 ug)
ACCESSION   NM_172075
VERSION     NM_172075.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3106..3197 
                     /label="Myc-DDK"  
     misc_feature    3227..3812 
                     /label="PolyA signal"  
     misc_feature    3284..3303 
                     /label="Restriction Enzyme"  
     misc_feature    4088..4760 
                     /label="ColE1"  
     misc_feature    5190..5984 
                     /label="Kan/Neo"  
     misc_feature    6019..6321 
                     /label="SV40 ori"  
     misc_feature    6407..6884 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3081..3088 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3081 
                     /label="Thop1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGCCCCCC GCAGCCTGTG CTGGGGACGT GGTGGATACA GTGTCACCAT 
     1081 GCTCCACCGT GAATCATCTG CGCTGGGACC TGAGCGCACA GCAGATCAGA GCTCTAACCA 
     1141 CACAGCTCAT CGAGCAGACC AAGTGTGTGT ACGACCGTGT GGGCGCCCAG GACTTCGAGG 
     1201 ACGTGTCCTA TGAGAGCACA CTGAAGGCAC TGGCTGACGT GGAGGTCACC TACACAGTGC 
     1261 AGAGGAACAT TCTCGACTTC CCCCAGCACG TGTCTCCGAA CAAGGACATC CGCGCAGCCA 
     1321 GCACAGAGGC TGACAAGAAG CTCTCAGAGT TTGATGTGGA GATGAGCATG AGGCAGGACG 
     1381 TGTACCAGAG GGTCGTGTGG CTGCAGGAGA AAATCCCGAA AGACTCACTG AAGCCTGAGG 
     1441 CAGCTCGCTA CCTGGAGCGG CTCATCAAGC TGGGCCGGAG AAACGGGCTC CACTTACCTC 
     1501 AGGACACACA GGAGAAGATC AAGAACATCA AGAAGAGGCT GAGCCTGCTG TGCATCGACT 
     1561 TCAACAAGAA CCTGAATGAG GACACCACCT TCCTGCCCTT CACGAGAGAG GAGCTGGGCG 
     1621 GGCTCCCCGA GGACTTCCTG AACTCCCTGG AGAAGACAGA GGACGGCAAA CTGAAGGTCA 
     1681 CCCTCAAGTA TCCACACTAT TTCCCACTGC TGAAGAAGTG CCACGTGCCC GAGACACGAC 
     1741 GCCTGTTGGA GGAGGCCTTC AACTGTCGCT GCAAGGAGGA GAACTGTGCC ATCCTGAAGG 
     1801 AGCTAGTGTC CCTGCGGGCG CAGAAGTCCA ACCTGCTGGG ATTCCGCACA CACGCAGACT 
     1861 ACGTCCTGGA GATGAACATG GCCAAGACCA GTCAGACAGT AGCCACCTTC CTAGATGAAC 
     1921 TGGCCCGGAA GCTGAAGCCG CTGGGTGAGC AGGAGCGTGC GGTGATCCTG GAGCTGAAGG 
     1981 AGGCAGAGTG CGCCAAGCGC GGGCTCCCAT TCGATGGGCG CATCCACGCA TGGGACATGC 
     2041 GGTACTACAT GAACCAGGTG GAGGAGACAC GCTACCGCGT AGACCAGAAC CTGCTGAAGG 
     2101 AGTACTTCCC CATGCAGGTG GTCACGCGTG GGCTGCTGGC CATCTACCAA GAGCTGCTGG 
     2161 GGCTGACCTT CACGCTGGAG GAAGGTGCCG CCGCCTGGCA CGAAGACGTG CGGCTGTACT 
     2221 CTGTGCGTGA CGCCGCCTCT GGAGAGGAGA TTGGCAAGTT CTACCTCGAC CTGTACCCCA 
     2281 GGGAAGGGAA GTATGGCCAC GCGGCCTGCT TCGGCCTGCA GCCTGGCTGC CTACGGCAGG 
     2341 ATGGCAGCCG ACAGCTGGCC ATCGCAGCCA TGGTGGCCAA TTTCACCAAG CCCACACCTG 
     2401 ACGTGCCTTC CCTGCTGCAG CACGATGAGG TGGAGACCTA CTTCCACGAG TTCGGGCACG 
     2461 TCATGCACCA GCTCTGCTCA CAGGCAGAGT TTGCTATGTT CAGTGGGACC CACGTGGAGC 
     2521 GGGACTTTGT GGAGGCACCG TCACAGATGC TGGAGAACTG GGTGTGGGAA AAGGAGCCAC 
     2581 TGATGCGCAT GTCCCAGCAT TACCGCACAG GAGGCGAGGC CCCTGAGGAC CTCCTGGAGA 
     2641 AGCTCATCAA GTCTCGCCAG GCCAATGCAG GTCTCTTCAA CCTTCGGCAG ATTGTCCTTG 
     2701 CCAAGGTAGA TCAGGTCCTG CACACACAGA CAGATGTAGA CCCAGCTGAG GAATATGCCC 
     2761 GGCTCTGCCA GGAGATCCTT GGGGTGCCAG CCACACCAGG TACCAACATG CCAGCCACTT 
     2821 TTGGCCACCT CGCTGGTGGC TACGACGCTC AGTACTATGG CTACTTGTGG AGTGAGGTGT 
     2881 ACTCCATGGA CATGTTCCAC ACACGCTTCA AGCAGGAGGG TGTGCTTAGC CCCAAGGTTG 
     2941 GCATGGATTA CCGGACCAGC ATCCTGAGGC CGGGGGGCTC TGAGGATGCC AGCACCATGC 
     3001 TGAAGCAGTT CCTGGGCCGT GACCCCAAGC AAGACGCCTT CCTCCTGAGC AAGGGTCTGC 
     3061 AGGTAGAGGG CTGCGAGCCA CCTGCGTGCA GCGGACCGAC GCGTACGCGG CCGCTCGAGC 
     3121 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     3181 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     3241 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     3301 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     3361 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     3421 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     3481 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     3541 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     3601 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     3661 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     3721 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     3781 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     3841 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     3901 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     3961 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     4021 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     4081 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     4141 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     4201 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     4261 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     4321 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     4381 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     4441 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     4501 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     4561 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     4621 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     4681 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     4741 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     4801 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     4861 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     4921 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     4981 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     5041 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     5101 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     5161 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     5221 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     5281 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     5341 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     5401 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     5461 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     5521 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     5581 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     5641 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     5701 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     5761 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     5821 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     5881 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     5941 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     6001 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     6061 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     6121 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     6181 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6241 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     6301 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     6361 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     6421 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     6481 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     6541 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     6601 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     6661 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     6721 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     6781 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     6841 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     6901 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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